Restriction map for
pFN21AE2094
(Linear) (Six-base) MAPSORT of: KIEE2094.seq Check: 8129 from: 1 to: 2388
KIEE2094.orf
With 182 enzymes: SgfI *
March 19, 2012 13:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 191 2388
Size: 191 2197
AccI GT'mk_AC
Cuts at: 0 2267 2369 2388
Size: 2267 102 19
Fragments arranged by size:
2267 102 19
Acc65I G'GTAC_C
Cuts at: 0 2183 2388
Size: 2183 205
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 607 1473 1657 2388
Size: 607 866 184 731
Fragments arranged by size:
866 731 607 184
AfeI AGC'GCT
Cuts at: 0 481 2388
Size: 481 1907
AflIII A'CryG_T
Cuts at: 0 2126 2388
Size: 2126 262
AhdI GACnn_n'nnGTC
Cuts at: 0 1987 2388
Size: 1987 401
AleI CACnn'nnGTG
Cuts at: 0 292 877 2388
Size: 292 585 1511
Fragments arranged by size:
1511 585 292
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2080 2229 2388
Size: 2080 149 159
Fragments arranged by size:
2080 159 149
AlwNI CAG_nnn'CTG
Cuts at: 0 234 243 426 522 1881 1983 2388
Size: 234 9 183 96 1359 102 405
Fragments arranged by size:
1359 405 234 183 102 96 9
ApaI G_GGCC'C
Cuts at: 0 165 545 2388
Size: 165 380 1843
Fragments arranged by size:
1843 380 165
ApaLI G'TGCA_C
Cuts at: 0 1939 2329 2388
Size: 1939 390 59
Fragments arranged by size:
1939 390 59
AvaI C'yCGr_G
Cuts at: 0 135 152 1159 2086 2388
Size: 135 17 1007 927 302
Fragments arranged by size:
1007 927 302 135 17
BamHI G'GATC_C
Cuts at: 0 680 2388
Size: 680 1708
BanI G'GyrC_C
Cuts at: 0 94 318 597 641 927 1555 2183 2358
Size: 94 224 279 44 286 628 628 175
Cuts at: 2358 2388
Size: 30
Fragments arranged by size:
628 628 286 279 224 175 94 44
30
BanII G_rGCy'C
Cuts at: 0 165 212 251 545 1457 2303 2388
Size: 165 47 39 294 912 846 85
Fragments arranged by size:
912 846 294 165 85 47 39
BbeI G_GCGC'C
Cuts at: 0 98 322 1559 2388
Size: 98 224 1237 829
Fragments arranged by size:
1237 829 224 98
BbsI GAAGACnn'nnnn_
Cuts at: 0 1563 2388
Size: 1563 825
BglI GCCn_nnn'nGGC
Cuts at: 0 128 2388
Size: 128 2260
BglII A'GATC_T
Cuts at: 0 2156 2388
Size: 2156 232
BlpI GC'TnA_GC
Cuts at: 0 1682 2388
Size: 1682 706
Bme1580I G_kGCm'C
Cuts at: 0 165 465 545 581 602 1943 2333 2388
Size: 165 300 80 36 21 1341 390 55
Fragments arranged by size:
1341 390 300 165 80 55 36 21
BmgBI CAC'GTC
Cuts at: 0 630 791 1059 2127 2230 2388
Size: 630 161 268 1068 103 158
Fragments arranged by size:
1068 630 268 161 158 103
BmrI ACTGGGnnnn_n'
Cuts at: 0 508 2388
Size: 508 1880
BmtI G_CTAG'C
Cuts at: 0 1895 2388
Size: 1895 493
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 405 2388
Size: 405 1983
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 265 400 685 715 1018 1232 1355 1838
Size: 265 135 285 30 303 214 123 483
Cuts at: 1838 2388
Size: 550
Fragments arranged by size:
550 483 303 285 265 214 135 123
30
Bpu10I CC'TnA_GC
Cuts at: 0 1601 2388
Size: 1601 787
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1519 2388
Size: 1519 869
BsaI GGTCTCn'nnnn_
Cuts at: 0 310 831 964 1009 1208 1257 2388
Size: 310 521 133 45 199 49 1131
Fragments arranged by size:
1131 521 310 199 133 49 45
BsaAI yAC'GTr
Cuts at: 0 2248 2388
Size: 2248 140
BsaHI Gr'CG_yC
Cuts at: 0 95 319 1556 1645 1730 2388
Size: 95 224 1237 89 85 658
Fragments arranged by size:
1237 658 224 95 89 85
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1205 1235 2176 2206 2388
Size: 1205 30 941 30 182
Fragments arranged by size:
1205 941 182 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 123 332 502 505 2388
Size: 123 209 170 3 1883
Fragments arranged by size:
1883 209 170 123 3
BseYI C'CCAG_C
Cuts at: 0 41 482 794 1545 2303 2388
Size: 41 441 312 751 758 85
Fragments arranged by size:
758 751 441 312 85 41
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 424 433 853 883 1719 2388
Size: 424 9 420 30 836 669
Fragments arranged by size:
836 669 424 420 30 9
BsiHKAI G_wGCw'C
Cuts at: 0 212 251 965 1316 1535 1659 1943 2150
Size: 212 39 714 351 219 124 284 207
Cuts at: 2150 2333 2388
Size: 183 55
Fragments arranged by size:
714 351 284 219 212 207 183 124
55 39
BsmBI CGTCTCn'nnnn_
Cuts at: 0 849 938 2388
Size: 849 89 1450
Fragments arranged by size:
1450 849 89
Bsp1286I G_dGCh'C
Cuts at: 0 165 212 251 465 545 581 602 965
Size: 165 47 39 214 80 36 21 363
Cuts at: 965 1316 1457 1535 1659 1943 2150 2303 2333
Size: 351 141 78 124 284 207 153 30
Cuts at: 2333 2388
Size: 55
Fragments arranged by size:
363 351 284 214 207 165 153 141
124 80 78 55 47 39 36 30
21
BspHI T'CATG_A
Cuts at: 0 1403 2388
Size: 1403 985
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 191 2388
Size: 191 2197
BsrBI CCG'CTC
Cuts at: 0 2241 2388
Size: 2241 147
BsrFI r'CCGG_y
Cuts at: 0 673 924 1002 1188 2388
Size: 673 251 78 186 1200
Fragments arranged by size:
1200 673 251 186 78
BstAPI GCAn_nnn'nTGC
Cuts at: 0 234 1716 1938 2388
Size: 234 1482 222 450
Fragments arranged by size:
1482 450 234 222
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1830 2388
Size: 1830 558
BstYI r'GATC_y
Cuts at: 0 680 2156 2388
Size: 680 1476 232
Fragments arranged by size:
1476 680 232
BtgI C'CryG_G
Cuts at: 0 126 707 1809 2388
Size: 126 581 1102 579
Fragments arranged by size:
1102 581 579 126
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 159 2388
Size: 159 2229
BtsI GCAGTG_nn'
Cuts at: 0 532 2388
Size: 532 1856
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1305 1340 2388
Size: 1305 35 1048
Fragments arranged by size:
1305 1048 35
DraIII CAC_nnn'GTG
Cuts at: 0 1716 2388
Size: 1716 672
EaeI y'GGCC_r
Cuts at: 0 752 854 1101 1271 1385 1952 2242 2388
Size: 752 102 247 170 114 567 290 146
Fragments arranged by size:
752 567 290 247 170 146 114 102
EarI CTCTTCn'nnn_
Cuts at: 0 292 952 1294 2388
Size: 292 660 342 1094
Fragments arranged by size:
1094 660 342 292
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 26 518 1101 2388
Size: 26 492 583 1287
Fragments arranged by size:
1287 583 492 26
EcoICRI GAG'CTC
Cuts at: 0 210 249 2388
Size: 210 39 2139
Fragments arranged by size:
2139 210 39
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 265 400 607 685 715 1018 1232 1355
Size: 265 135 207 78 30 303 214 123
Cuts at: 1355 1473 1657 1838 2388
Size: 118 184 181 550
Fragments arranged by size:
550 303 265 214 207 184 181 135
123 118 78 30
EcoNI CCTnn'n_nnAGG
Cuts at: 0 667 2353 2388
Size: 667 1686 35
Fragments arranged by size:
1686 667 35
EcoO109I rG'GnC_Cy
Cuts at: 0 161 162 264 408 541 542 660 1164
Size: 161 1 102 144 133 1 118 504
Cuts at: 1164 1785 1858 1947 2388
Size: 621 73 89 441
Fragments arranged by size:
621 504 441 161 144 133 118 102
89 73 1 1
EcoRV GAT'ATC
Cuts at: 0 2022 2388
Size: 2022 366
HaeII r_GCGC'y
Cuts at: 0 98 322 483 1521 1559 2388
Size: 98 224 161 1038 38 829
Fragments arranged by size:
1038 829 224 161 98 38
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1973 2005 2388
Size: 1973 32 383
Fragments arranged by size:
1973 383 32
HincII GTy'rAC
Cuts at: 0 840 1062 2388
Size: 840 222 1326
Fragments arranged by size:
1326 840 222
KasI G'GCGC_C
Cuts at: 0 94 318 1555 2388
Size: 94 224 1237 833
Fragments arranged by size:
1237 833 224 94
KpnI G_GTAC'C
Cuts at: 0 2187 2388
Size: 2187 201
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2216 2219 2388
Size: 2216 3 169
Fragments arranged by size:
2216 169 3
MscI TGG'CCA
Cuts at: 0 754 1387 1954 2388
Size: 754 633 567 434
Fragments arranged by size:
754 633 567 434
MslI CAynn'nnrTG
Cuts at: 0 292 712 877 2388
Size: 292 420 165 1511
Fragments arranged by size:
1511 420 292 165
MspA1I CmG'CkG
Cuts at: 0 41 569 867 1878 2295 2388
Size: 41 528 298 1011 417 93
Fragments arranged by size:
1011 528 417 298 93 41
NaeI GCC'GGC
Cuts at: 0 675 926 1004 2388
Size: 675 251 78 1384
Fragments arranged by size:
1384 675 251 78
NarI GG'CG_CC
Cuts at: 0 95 319 1556 2388
Size: 95 224 1237 832
Fragments arranged by size:
1237 832 224 95
NcoI C'CATG_G
Cuts at: 0 1809 2388
Size: 1809 579
NgoMIV G'CCGG_C
Cuts at: 0 673 924 1002 2388
Size: 673 251 78 1386
Fragments arranged by size:
1386 673 251 78
NheI G'CTAG_C
Cuts at: 0 1891 2388
Size: 1891 497
NspI r_CATG'y
Cuts at: 0 558 2388
Size: 558 1830
PasI CC'CwG_GG
Cuts at: 0 738 1863 2388
Size: 738 1125 525
Fragments arranged by size:
1125 738 525
PflMI CCAn_nnn'nTGG
Cuts at: 0 1378 1983 2075 2388
Size: 1378 605 92 313
Fragments arranged by size:
1378 605 313 92
PfoI T'CCnGG_A
Cuts at: 0 391 995 2388
Size: 391 604 1393
Fragments arranged by size:
1393 604 391
PmlI CAC'GTG
Cuts at: 0 2248 2388
Size: 2248 140
PpuMI rG'GwC_Cy
Cuts at: 0 1164 1785 1947 2388
Size: 1164 621 162 441
Fragments arranged by size:
1164 621 441 162
PspOMI G'GGCC_C
Cuts at: 0 161 541 2388
Size: 161 380 1847
Fragments arranged by size:
1847 380 161
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1468 1500 2092 2124 2388
Size: 1468 32 592 32 264
Fragments arranged by size:
1468 592 264 32 32
PstI C_TGCA'G
Cuts at: 0 239 527 1703 2388
Size: 239 288 1176 685
Fragments arranged by size:
1176 685 288 239
PvuII CAG'CTG
Cuts at: 0 41 569 867 1878 2388
Size: 41 528 298 1011 510
Fragments arranged by size:
1011 528 510 298 41
SacI G_AGCT'C
Cuts at: 0 212 251 2388
Size: 212 39 2137
Fragments arranged by size:
2137 212 39
SapI GCTCTTCn'nnn_
Cuts at: 0 952 2388
Size: 952 1436
SfcI C'TryA_G
Cuts at: 0 235 475 523 1699 2025 2388
Size: 235 240 48 1176 326 363
Fragments arranged by size:
1176 363 326 240 235 48
SfoI GGC'GCC
Cuts at: 0 96 320 1557 2388
Size: 96 224 1237 831
Fragments arranged by size:
1237 831 224 96
SgrAI Cr'CCGG_yG
Cuts at: 0 1188 2388
Size: 1188 1200
SmaI CCC'GGG
Cuts at: 0 154 2388
Size: 154 2234
SmlI C'TyrA_G
Cuts at: 0 1534 2388
Size: 1534 854
StuI AGG'CCT
Cuts at: 0 1108 2388
Size: 1108 1280
StyI C'CwwG_G
Cuts at: 0 1458 1809 2388
Size: 1458 351 579
Fragments arranged by size:
1458 579 351
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1069 1402 2388
Size: 1069 333 986
Fragments arranged by size:
1069 986 333
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1205 1237 2388
Size: 1205 32 1151
Fragments arranged by size:
1205 1151 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 609 2388
Size: 609 1779
XmaI C'CCGG_G
Cuts at: 0 152 2388
Size: 152 2236
XmnI GAAnn'nnTTC
Cuts at: 0 1570 2388
Size: 1570 818
Enzymes that do cut:
AarI AccI Acc65I AcuI AfeI AflIII AhdI AleI
AlfI AlwNI ApaI ApaLI AvaI BamHI BanI BanII
BbeI BbsI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI
BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspHI
BspMI BsrBI BsrFI BstAPI BstXI BstYI BtgI BtgZI
BtsI CspCI DraIII EaeI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRV HaeII Hin4I HincII KasI KpnI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NheI NspI PasI PflMI PfoI PmlI PpuMI PspOMI
PsrI PstI PvuII SacI SapI SfcI SfoI SgrAI
SmaI SmlI StuI StyI TaqII TstI XcmI XmaI
XmnI
Enzymes that do not cut:
AatII AclI AflII AgeI AloI ApoI AscI AseI
AsiSI AvrII BaeI BbvCI BcgI BciVI BclI BfrBI
BsaBI BsaWI BsiEI BsiWI BsmI BspEI BsrDI BsrGI
BssHII BssSI BstBI BstEII BstZ17I Bsu36I ClaI DraI
DrdI EagI EcoRI FalI FseI FspI FspAI HindIII
HpaI MfeI MluI NdeI NotI NruI NsiI PacI
PciI PmeI PpiI PshAI PsiI PspXI PvuI RsrII
SacII SalI SanDI SbfI ScaI SexAI SfiI SgfI
SnaBI SpeI SphI SrfI SspI SwaI TatI Tth111I
XbaI XhoI ZraI