Restriction map for
pFN21AE2092
(Linear) (Six-base) MAPSORT of: KIEE2092.seq Check: 2238 from: 1 to: 2346
KIEE2092.orf
With 182 enzymes: SgfI *
March 22, 2012 15:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1625 2346
Size: 1625 721
AatII G_ACGT'C
Cuts at: 0 1657 2346
Size: 1657 689
AccI GT'mk_AC
Cuts at: 0 1316 2346
Size: 1316 1030
Acc65I G'GTAC_C
Cuts at: 0 1766 2346
Size: 1766 580
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 34 1387 1753 1971 2290 2316 2346
Size: 34 1353 366 218 319 26 30
Fragments arranged by size:
1353 366 319 218 34 30 26
AfeI AGC'GCT
Cuts at: 0 2020 2100 2346
Size: 2020 80 246
Fragments arranged by size:
2020 246 80
AflIII A'CryG_T
Cuts at: 0 1820 2346
Size: 1820 526
AhdI GACnn_n'nnGTC
Cuts at: 0 786 1824 2346
Size: 786 1038 522
Fragments arranged by size:
1038 786 522
AleI CACnn'nnGTG
Cuts at: 0 1813 2346
Size: 1813 533
AlwNI CAG_nnn'CTG
Cuts at: 0 689 868 1224 1281 1599 2346
Size: 689 179 356 57 318 747
Fragments arranged by size:
747 689 356 318 179 57
ApaI G_GGCC'C
Cuts at: 0 2326 2346
Size: 2326 20
ApoI r'AATT_y
Cuts at: 0 44 108 2346
Size: 44 64 2238
Fragments arranged by size:
2238 64 44
AvaI C'yCGr_G
Cuts at: 0 1876 2083 2217 2346
Size: 1876 207 134 129
Fragments arranged by size:
1876 207 134 129
BamHI G'GATC_C
Cuts at: 0 1631 1723 2346
Size: 1631 92 623
Fragments arranged by size:
1631 623 92
BanI G'GyrC_C
Cuts at: 0 1300 1766 1792 2209 2346
Size: 1300 466 26 417 137
Fragments arranged by size:
1300 466 417 137 26
BanII G_rGCy'C
Cuts at: 0 1214 1964 2031 2326 2346
Size: 1214 750 67 295 20
Fragments arranged by size:
1214 750 295 67 20
BbsI GAAGACnn'nnnn_
Cuts at: 0 215 227 829 1585 2346
Size: 215 12 602 756 761
Fragments arranged by size:
761 756 602 215 12
BbvCI CC'TCA_GC
Cuts at: 0 1408 2102 2346
Size: 1408 694 244
Fragments arranged by size:
1408 694 244
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 256 290 379 413 1174 1208 2004 2038
Size: 256 34 89 34 761 34 796 34
Cuts at: 2038 2346
Size: 308
Fragments arranged by size:
796 761 308 256 89 34 34 34
34
BglI GCCn_nnn'nGGC
Cuts at: 0 1679 2346
Size: 1679 667
BglII A'GATC_T
Cuts at: 0 1493 2346
Size: 1493 853
BlpI GC'TnA_GC
Cuts at: 0 1669 2346
Size: 1669 677
Bme1580I G_kGCm'C
Cuts at: 0 1184 1303 2212 2326 2346
Size: 1184 119 909 114 20
Fragments arranged by size:
1184 909 119 114 20
BmgBI CAC'GTC
Cuts at: 0 1403 2346
Size: 1403 943
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 711 2346
Size: 711 1635
Bpu10I CC'TnA_GC
Cuts at: 0 861 1408 2102 2346
Size: 861 547 694 244
Fragments arranged by size:
861 694 547 244
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 163 1198 1414 1513 1633 1672 2098 2323
Size: 163 1035 216 99 120 39 426 225
Cuts at: 2323 2346
Size: 23
Fragments arranged by size:
1035 426 225 216 163 120 99 39
23
BsaI GGTCTCn'nnnn_
Cuts at: 0 961 1537 2128 2346
Size: 961 576 591 218
Fragments arranged by size:
961 591 576 218
BsaAI yAC'GTr
Cuts at: 0 2171 2346
Size: 2171 175
BsaBI GATnn'nnATC
Cuts at: 0 1498 2346
Size: 1498 848
BsaHI Gr'CG_yC
Cuts at: 0 1654 2346
Size: 1654 692
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 903 933 1261 1291 2346
Size: 903 30 328 30 1055
Fragments arranged by size:
1055 903 328 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 94 160 242 610 895 898 901 910
Size: 94 66 82 368 285 3 3 9
Cuts at: 910 913 916 946 949 952 955 970 973
Size: 3 3 30 3 3 3 15 3
Cuts at: 973 976 979 1000 1282 1309 2103 2346
Size: 3 3 21 282 27 794 243
Fragments arranged by size:
794 368 285 282 243 94 82 66
30 27 21 15 9 3 3 3
3 3 3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 0 263 1436 1965 2253 2346
Size: 263 1173 529 288 93
Fragments arranged by size:
1173 529 288 263 93
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 620 1603 1621 2346
Size: 620 983 18 725
Fragments arranged by size:
983 725 620 18
BsiEI CG_ry'CG
Cuts at: 0 712 744 836 1095 1751 1814 2346
Size: 712 32 92 259 656 63 532
Fragments arranged by size:
712 656 532 259 92 63 32
BsiHKAI G_wGCw'C
Cuts at: 0 392 916 1214 1523 1964 1997 2346
Size: 392 524 298 309 441 33 349
Fragments arranged by size:
524 441 392 349 309 298 33
BsmI GAATG_Cn'
Cuts at: 0 477 2346
Size: 477 1869
BsmBI CGTCTCn'nnnn_
Cuts at: 0 731 2269 2346
Size: 731 1538 77
Fragments arranged by size:
1538 731 77
Bsp1286I G_dGCh'C
Cuts at: 0 392 916 1184 1214 1303 1523 1964 1997
Size: 392 524 268 30 89 220 441 33
Cuts at: 1997 2031 2212 2326 2346
Size: 34 181 114 20
Fragments arranged by size:
524 441 392 268 220 181 114 89
34 33 30 20
BspHI T'CATG_A
Cuts at: 0 2057 2346
Size: 2057 289
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 791 1244 1625 1670 2346
Size: 791 453 381 45 676
Fragments arranged by size:
791 676 453 381 45
BsrBI CCG'CTC
Cuts at: 0 528 585 600 612 723 2346
Size: 528 57 15 12 111 1623
Fragments arranged by size:
1623 528 111 57 15 12
BsrFI r'CCGG_y
Cuts at: 0 970 1239 1678 2346
Size: 970 269 439 668
Fragments arranged by size:
970 668 439 269
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1224 2346
Size: 1224 1122
BstEII G'GTnAC_C
Cuts at: 0 1755 2346
Size: 1755 591
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1815 2346
Size: 1815 531
BstYI r'GATC_y
Cuts at: 0 1493 1631 1723 1917 2346
Size: 1493 138 92 194 429
Fragments arranged by size:
1493 429 194 138 92
Bsu36I CC'TnA_GG
Cuts at: 0 2228 2346
Size: 2228 118
BtgI C'CryG_G
Cuts at: 0 715 1808 1814 2318 2346
Size: 715 1093 6 504 28
Fragments arranged by size:
1093 715 504 28 6
ClaI AT'CG_AT
Cuts at: 0 1892 2346
Size: 1892 454
EaeI y'GGCC_r
Cuts at: 0 248 709 741 833 1811 2346
Size: 248 461 32 92 978 535
Fragments arranged by size:
978 535 461 248 92 32
EagI C'GGCC_G
Cuts at: 0 709 741 833 1811 2346
Size: 709 32 92 978 535
Fragments arranged by size:
978 709 535 92 32
EarI CTCTTCn'nnn_
Cuts at: 0 198 448 755 872 887 941 947 2346
Size: 198 250 307 117 15 54 6 1399
Fragments arranged by size:
1399 307 250 198 117 54 15 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 607 2346
Size: 607 1739
EcoICRI GAG'CTC
Cuts at: 0 1212 1962 2346
Size: 1212 750 384
Fragments arranged by size:
1212 750 384
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 34 711 1387 1753 1971 2290 2316 2346
Size: 34 677 676 366 218 319 26 30
Fragments arranged by size:
677 676 366 319 218 34 30 26
EcoNI CCTnn'n_nnAGG
Cuts at: 0 145 1165 2231 2346
Size: 145 1020 1066 115
Fragments arranged by size:
1066 1020 145 115
EcoO109I rG'GnC_Cy
Cuts at: 0 780 1925 2222 2309 2323 2346
Size: 780 1145 297 87 14 23
Fragments arranged by size:
1145 780 297 87 23 14
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1407 2001 2346
Size: 1407 594 345
Fragments arranged by size:
1407 594 345
HaeII r_GCGC'y
Cuts at: 0 1160 2022 2102 2346
Size: 1160 862 80 244
Fragments arranged by size:
1160 862 244 80
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 772 804 829 861 976 1008 1708 1740
Size: 772 32 25 32 115 32 700 32
Cuts at: 1740 1810 1842 2346
Size: 70 32 504
Fragments arranged by size:
772 700 504 115 70 32 32 32
32 32 25
KpnI G_GTAC'C
Cuts at: 0 1770 2346
Size: 1770 576
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 379 815 1682 2346
Size: 379 436 867 664
Fragments arranged by size:
867 664 436 379
MscI TGG'CCA
Cuts at: 0 250 2346
Size: 250 2096
MslI CAynn'nnrTG
Cuts at: 0 1504 1813 2346
Size: 1504 309 533
Fragments arranged by size:
1504 533 309
MspA1I CmG'CkG
Cuts at: 0 564 717 850 1596 2253 2320 2346
Size: 564 153 133 746 657 67 26
Fragments arranged by size:
746 657 564 153 133 67 26
NaeI GCC'GGC
Cuts at: 0 1241 1680 2346
Size: 1241 439 666
Fragments arranged by size:
1241 666 439
NgoMIV G'CCGG_C
Cuts at: 0 1239 1678 2346
Size: 1239 439 668
Fragments arranged by size:
1239 668 439
NspI r_CATG'y
Cuts at: 0 364 1824 2346
Size: 364 1460 522
Fragments arranged by size:
1460 522 364
PciI A'CATG_T
Cuts at: 0 1820 2346
Size: 1820 526
PflMI CCAn_nnn'nTGG
Cuts at: 0 689 1068 2346
Size: 689 379 1278
Fragments arranged by size:
1278 689 379
PfoI T'CCnGG_A
Cuts at: 0 481 2346
Size: 481 1865
PmlI CAC'GTG
Cuts at: 0 2171 2346
Size: 2171 175
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1072 1104 2346
Size: 1072 32 1242
Fragments arranged by size:
1242 1072 32
PpuMI rG'GwC_Cy
Cuts at: 0 780 1925 2346
Size: 780 1145 421
Fragments arranged by size:
1145 780 421
PspOMI G'GGCC_C
Cuts at: 0 2322 2346
Size: 2322 24
PstI C_TGCA'G
Cuts at: 0 1250 2346
Size: 1250 1096
PvuII CAG'CTG
Cuts at: 0 1596 2253 2346
Size: 1596 657 93
Fragments arranged by size:
1596 657 93
SacI G_AGCT'C
Cuts at: 0 1214 1964 2346
Size: 1214 750 382
Fragments arranged by size:
1214 750 382
SacII CC_GC'GG
Cuts at: 0 718 2321 2346
Size: 718 1603 25
Fragments arranged by size:
1603 718 25
SanDI GG'GwC_CC
Cuts at: 0 1925 2346
Size: 1925 421
SbfI CC_TGCA'GG
Cuts at: 0 1250 2346
Size: 1250 1096
SfcI C'TryA_G
Cuts at: 0 663 1246 1317 1953 2244 2346
Size: 663 583 71 636 291 102
Fragments arranged by size:
663 636 583 291 102 71
SmaI CCC'GGG
Cuts at: 0 1878 2085 2346
Size: 1878 207 261
Fragments arranged by size:
1878 261 207
SmlI C'TyrA_G
Cuts at: 0 142 1213 1429 1528 1648 1687 2113 2338
Size: 142 1071 216 99 120 39 426 225
Cuts at: 2338 2346
Size: 8
Fragments arranged by size:
1071 426 225 216 142 120 99 39
8
SphI G_CATG'C
Cuts at: 0 364 2346
Size: 364 1982
StuI AGG'CCT
Cuts at: 0 1738 2237 2346
Size: 1738 499 109
Fragments arranged by size:
1738 499 109
StyI C'CwwG_G
Cuts at: 0 1796 2346
Size: 1796 550
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1109 1123 1684 2245 2346
Size: 1109 14 561 561 101
Fragments arranged by size:
1109 561 561 101 14
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 903 935 1261 1293 1917 1949 2346
Size: 903 32 326 32 624 32 397
Fragments arranged by size:
903 624 397 326 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1600 2346
Size: 1600 746
XmaI C'CCGG_G
Cuts at: 0 1876 2083 2346
Size: 1876 207 263
Fragments arranged by size:
1876 263 207
XmnI GAAnn'nnTTC
Cuts at: 0 47 2065 2346
Size: 47 2018 281
Fragments arranged by size:
2018 281 47
ZraI GAC'GTC
Cuts at: 0 1655 2346
Size: 1655 691
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AfeI AflIII AhdI
AleI AlwNI ApaI ApoI AvaI BamHI BanI BanII
BbsI BbvCI BcgI BglI BglII BlpI Bme1580I BmgBI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspHI BspMI BsrBI BsrFI BstAPI BstEII BstXI BstYI
Bsu36I BtgI ClaI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I FalI HaeII Hin4I KpnI MmeI
MscI MslI MspA1I NaeI NgoMIV NspI PciI PflMI
PfoI PmlI PpiI PpuMI PspOMI PstI PvuII SacI
SacII SanDI SbfI SfcI SmaI SmlI SphI StuI
StyI TaqII TstI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AclI AflII AgeI AlfI AloI ApaLI AscI AseI
AsiSI AvrII BaeI BbeI BciVI BclI BfrBI BmrI
BmtI BplI BsaWI BsiWI BspEI BsrDI BsrGI BssHII
BssSI BstBI BstZ17I BtgZI BtsI CspCI DraI DraIII
DrdI EcoRI EcoRV FseI FspI FspAI HincII HindIII
HpaI KasI MfeI MluI NarI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PmeI PshAI PsiI
PspXI PsrI PvuI RsrII SalI SapI ScaI SexAI
SfiI SfoI SgfI SgrAI SnaBI SpeI SrfI SspI
SwaI TatI Tth111I XbaI XhoI