Restriction map for
pFN21AE2090
(Linear) (Six-base) MAPSORT of: KIEE2090.seq Check: 2205 from: 1 to: 2307
KIEE2090.orf
With 182 enzymes: SgfI *
July 3, 2012 17:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2238 2307
Size: 2238 69
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 167 593 1711 2000 2307
Size: 167 426 1118 289 307
Fragments arranged by size:
1118 426 307 289 167
AflII C'TTAA_G
Cuts at: 0 2242 2307
Size: 2242 65
AflIII A'CryG_T
Cuts at: 0 1181 2307
Size: 1181 1126
AgeI A'CCGG_T
Cuts at: 0 1981 2307
Size: 1981 326
AleI CACnn'nnGTG
Cuts at: 0 1566 2307
Size: 1566 741
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 464 2307
Size: 464 1843
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 209 241 1580 1612 2307
Size: 209 32 1339 32 695
Fragments arranged by size:
1339 695 209 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 517 2253 2307
Size: 517 1736 54
Fragments arranged by size:
1736 517 54
ApoI r'AATT_y
Cuts at: 0 45 378 800 975 1146 1705 1757 1872
Size: 45 333 422 175 171 559 52 115
Cuts at: 1872 2124 2307
Size: 252 183
Fragments arranged by size:
559 422 333 252 183 175 171 115
52 45
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1154 1187 2307
Size: 1154 33 1120
Fragments arranged by size:
1154 1120 33
BanI G'GyrC_C
Cuts at: 0 1630 2307
Size: 1630 677
BbsI GAAGACnn'nnnn_
Cuts at: 0 15 143 499 917 2244 2307
Size: 15 128 356 418 1327 63
Fragments arranged by size:
1327 418 356 128 63 15
BglI GCCn_nnn'nGGC
Cuts at: 0 543 2307
Size: 543 1764
Bme1580I G_kGCm'C
Cuts at: 0 1635 2307
Size: 1635 672
BmrI ACTGGGnnnn_n'
Cuts at: 0 2182 2307
Size: 2182 125
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 512 845 1151 1495 1799 2307
Size: 512 333 306 344 304 508
Fragments arranged by size:
512 508 344 333 306 304
Bpu10I CC'TnA_GC
Cuts at: 0 1996 2307
Size: 1996 311
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 836 1646 2307
Size: 836 810 661
Fragments arranged by size:
836 810 661
BsaI GGTCTCn'nnnn_
Cuts at: 0 1574 2307
Size: 1574 733
BsaWI w'CCGG_w
Cuts at: 0 1981 2199 2307
Size: 1981 218 108
Fragments arranged by size:
1981 218 108
BseYI C'CCAG_C
Cuts at: 0 1295 2307
Size: 1295 1012
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 634 2307
Size: 634 1673
BsmI GAATG_Cn'
Cuts at: 0 846 1947 2307
Size: 846 1101 360
Fragments arranged by size:
1101 846 360
Bsp1286I G_dGCh'C
Cuts at: 0 1635 2307
Size: 1635 672
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2238 2307
Size: 2238 69
BsrDI GCAATG_nn'
Cuts at: 0 598 2307
Size: 598 1709
BsrFI r'CCGG_y
Cuts at: 0 1981 2307
Size: 1981 326
BssSI C'ACGA_G
Cuts at: 0 2043 2307
Size: 2043 264
BstXI CCAn_nnnn'nTGG
Cuts at: 0 869 2307
Size: 869 1438
BstYI r'GATC_y
Cuts at: 0 1595 2307
Size: 1595 712
Bsu36I CC'TnA_GG
Cuts at: 0 651 2307
Size: 651 1656
BtgI C'CryG_G
Cuts at: 0 1307 2307
Size: 1307 1000
ClaI AT'CG_AT
Cuts at: 0 1019 2307
Size: 1019 1288
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 917 952 2307
Size: 917 35 1355
Fragments arranged by size:
1355 917 35
DraI TTT'AAA
Cuts at: 0 953 1710 2307
Size: 953 757 597
Fragments arranged by size:
953 757 597
DrdI GACnn_nn'nnGTC
Cuts at: 0 522 2307
Size: 522 1785
EaeI y'GGCC_r
Cuts at: 0 1310 2307
Size: 1310 997
EarI CTCTTCn'nnn_
Cuts at: 0 264 1687 1921 2307
Size: 264 1423 234 386
Fragments arranged by size:
1423 386 264 234
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 167 512 593 845 1151 1495 1711 1799
Size: 167 345 81 252 306 344 216 88
Cuts at: 1799 2000 2307
Size: 201 307
Fragments arranged by size:
345 344 307 306 252 216 201 167
88 81
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1729 2307
Size: 1729 578
EcoRI G'AATT_C
Cuts at: 0 1146 1757 2124 2307
Size: 1146 611 367 183
Fragments arranged by size:
1146 611 367 183
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1277 2290 2307
Size: 1277 1013 17
Fragments arranged by size:
1277 1013 17
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1188 1220 1264 1296 1333 1365 1597 1629
Size: 1188 32 44 32 37 32 232 32
Cuts at: 1629 2307
Size: 678
Fragments arranged by size:
1188 678 232 44 37 32 32 32
32
MfeI C'AATT_G
Cuts at: 0 280 829 2307
Size: 280 549 1478
Fragments arranged by size:
1478 549 280
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1419 2307
Size: 1419 888
MscI TGG'CCA
Cuts at: 0 1312 2307
Size: 1312 995
MslI CAynn'nnrTG
Cuts at: 0 1566 2307
Size: 1566 741
NcoI C'CATG_G
Cuts at: 0 1307 2307
Size: 1307 1000
NdeI CA'TA_TG
Cuts at: 0 1373 2307
Size: 1373 934
NspI r_CATG'y
Cuts at: 0 84 2307
Size: 84 2223
PsiI TTA'TAA
Cuts at: 0 1949 2307
Size: 1949 358
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 321 353 2307
Size: 321 32 1954
Fragments arranged by size:
1954 321 32
SapI GCTCTTCn'nnn_
Cuts at: 0 264 2307
Size: 264 2043
SfcI C'TryA_G
Cuts at: 0 818 889 2307
Size: 818 71 1418
Fragments arranged by size:
1418 818 71
SmlI C'TyrA_G
Cuts at: 0 851 1661 2242 2307
Size: 851 810 581 65
Fragments arranged by size:
851 810 581 65
SpeI A'CTAG_T
Cuts at: 0 171 2307
Size: 171 2136
SspI AAT'ATT
Cuts at: 0 1239 2307
Size: 1239 1068
StuI AGG'CCT
Cuts at: 0 667 2307
Size: 667 1640
StyI C'CwwG_G
Cuts at: 0 1307 1635 2307
Size: 1307 328 672
Fragments arranged by size:
1307 672 328
TatI w'GTAC_w
Cuts at: 0 1447 2307
Size: 1447 860
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 786 818 2307
Size: 786 32 1489
Fragments arranged by size:
1489 786 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1774 2307
Size: 1774 533
XmnI GAAnn'nnTTC
Cuts at: 0 381 2216 2307
Size: 381 1835 91
Fragments arranged by size:
1835 381 91
Enzymes that do cut:
AarI AcuI AflII AflIII AgeI AleI AlfI AloI
AlwNI ApoI BaeI BanI BbsI BglI Bme1580I BmrI
BpmI Bpu10I BpuEI BsaI BsaWI BseYI BsgI BsmI
Bsp1286I BspMI BsrDI BsrFI BssSI BstXI BstYI Bsu36I
BtgI ClaI CspCI DraI DrdI EaeI EarI Eco57MI
EcoNI EcoRI FalI Hin4I MfeI MmeI MscI MslI
NcoI NdeI NspI PsiI PsrI SapI SfcI SmlI
SpeI SspI StuI StyI TatI TstI XcmI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AhdI ApaI ApaLI
AscI AseI AsiSI AvaI AvrII BamHI BanII BbeI
BbvCI BcgI BciVI BclI BfrBI BglII BlpI BmgBI
BmtI BplI BsaAI BsaBI BsaHI BsaXI BseRI BsiEI
BsiHKAI BsiWI BsmBI BspEI BspHI BsrBI BsrGI BssHII
BstAPI BstBI BstEII BstZ17I BtgZI BtsI DraIII EagI
EciI EcoICRI EcoO109I EcoRV FseI FspI FspAI HaeII
HincII HindIII HpaI KasI KpnI MluI MspA1I NaeI
NarI NgoMIV NheI NotI NruI NsiI PacI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PspOMI PspXI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SphI SrfI SwaI TaqII Tth111I
XbaI XhoI XmaI ZraI