Restriction map for
pFN21AE2089
(Linear) (Six-base) MAPSORT of: KIEE2089.seq Check: 8716 from: 1 to: 2292
KIEE2089.orf
With 182 enzymes: SgfI *
July 15, 2010 18:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 28 2292
Size: 28 2264
AccI GT'mk_AC
Cuts at: 0 416 2006 2292
Size: 416 1590 286
Fragments arranged by size:
1590 416 286
Acc65I G'GTAC_C
Cuts at: 0 1395 2292
Size: 1395 897
AfeI AGC'GCT
Cuts at: 0 1916 2292
Size: 1916 376
AleI CACnn'nnGTG
Cuts at: 0 277 1072 1414 1930 2292
Size: 277 795 342 516 362
Fragments arranged by size:
795 516 362 342 277
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 907 2292
Size: 907 1385
AlwNI CAG_nnn'CTG
Cuts at: 0 603 1515 1738 2292
Size: 603 912 223 554
Fragments arranged by size:
912 603 554 223
ApaI G_GGCC'C
Cuts at: 0 781 870 1170 2292
Size: 781 89 300 1122
Fragments arranged by size:
1122 781 300 89
ApoI r'AATT_y
Cuts at: 0 802 1459 2071 2292
Size: 802 657 612 221
Fragments arranged by size:
802 657 612 221
AvaI C'yCGr_G
Cuts at: 0 73 718 724 2292
Size: 73 645 6 1568
Fragments arranged by size:
1568 645 73 6
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1614 1647 2292
Size: 1614 33 645
Fragments arranged by size:
1614 645 33
BamHI G'GATC_C
Cuts at: 0 887 1800 2292
Size: 887 913 492
Fragments arranged by size:
913 887 492
BanI G'GyrC_C
Cuts at: 0 164 1264 1395 1932 2292
Size: 164 1100 131 537 360
Fragments arranged by size:
1100 537 360 164 131
BanII G_rGCy'C
Cuts at: 0 398 725 754 781 870 981 1170 1989
Size: 398 327 29 27 89 111 189 819
Cuts at: 1989 2292
Size: 303
Fragments arranged by size:
819 398 327 303 189 111 89 29
27
BbsI GAAGACnn'nnnn_
Cuts at: 0 27 746 2292
Size: 27 719 1546
Fragments arranged by size:
1546 719 27
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1530 1564 2292
Size: 1530 34 728
Fragments arranged by size:
1530 728 34
BclI T'GATC_A
Cuts at: 0 932 2292
Size: 932 1360
BglI GCCn_nnn'nGGC
Cuts at: 0 2221 2266 2292
Size: 2221 45 26
Fragments arranged by size:
2221 45 26
BlpI GC'TnA_GC
Cuts at: 0 298 634 2292
Size: 298 336 1658
Fragments arranged by size:
1658 336 298
Bme1580I G_kGCm'C
Cuts at: 0 774 781 870 1170 1937 2292
Size: 774 7 89 300 767 355
Fragments arranged by size:
774 767 355 300 89 7
BmgBI CAC'GTC
Cuts at: 0 840 2292
Size: 840 1452
BmrI ACTGGGnnnn_n'
Cuts at: 0 1215 2292
Size: 1215 1077
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1447 2292
Size: 1447 845
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 207 394 519 1267 1365 1893 2049 2227
Size: 207 187 125 748 98 528 156 178
Cuts at: 2227 2292
Size: 65
Fragments arranged by size:
748 528 207 187 178 156 125 98
65
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1375 1417 2292
Size: 1375 42 875
Fragments arranged by size:
1375 875 42
BsaI GGTCTCn'nnnn_
Cuts at: 0 1926 2164 2292
Size: 1926 238 128
Fragments arranged by size:
1926 238 128
BsaAI yAC'GTr
Cuts at: 0 116 2292
Size: 116 2176
BsaHI Gr'CG_yC
Cuts at: 0 1304 2292
Size: 1304 988
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1082 1112 2292
Size: 1082 30 1180
Fragments arranged by size:
1180 1082 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 91 195 1057 1264 1455 2292
Size: 91 104 862 207 191 837
Fragments arranged by size:
862 837 207 191 104 91
BseYI C'CCAG_C
Cuts at: 0 39 293 643 664 870 1419 1519 1537
Size: 39 254 350 21 206 549 100 18
Cuts at: 1537 2292
Size: 755
Fragments arranged by size:
755 549 350 254 206 100 39 21
18
BsiEI CG_ry'CG
Cuts at: 0 2010 2292
Size: 2010 282
BsiHKAI G_wGCw'C
Cuts at: 0 17 1109 2164 2292
Size: 17 1092 1055 128
Fragments arranged by size:
1092 1055 128 17
BsiWI C'GTAC_G
Cuts at: 0 1469 2292
Size: 1469 823
BsmI GAATG_Cn'
Cuts at: 0 477 699 2292
Size: 477 222 1593
Fragments arranged by size:
1593 477 222
BsmBI CGTCTCn'nnnn_
Cuts at: 0 225 2292
Size: 225 2067
Bsp1286I G_dGCh'C
Cuts at: 0 17 398 725 754 774 781 870 981
Size: 17 381 327 29 20 7 89 111
Cuts at: 981 1109 1170 1937 1989 2164 2292
Size: 128 61 767 52 175 128
Fragments arranged by size:
767 381 327 175 128 128 111 89
61 52 29 20 17 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 28 2292
Size: 28 2264
BsrDI GCAATG_nn'
Cuts at: 0 287 2292
Size: 287 2005
BsrFI r'CCGG_y
Cuts at: 0 143 167 1306 1571 2292
Size: 143 24 1139 265 721
Fragments arranged by size:
1139 721 265 143 24
BsrGI T'GTAC_A
Cuts at: 0 494 2292
Size: 494 1798
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1738 2292
Size: 1738 554
BstEII G'GTnAC_C
Cuts at: 0 441 1599 2292
Size: 441 1158 693
Fragments arranged by size:
1158 693 441
BstXI CCAn_nnnn'nTGG
Cuts at: 0 391 988 1654 1917 2256 2292
Size: 391 597 666 263 339 36
Fragments arranged by size:
666 597 391 339 263 36
BstYI r'GATC_y
Cuts at: 0 887 1800 2292
Size: 887 913 492
Fragments arranged by size:
913 887 492
Bsu36I CC'TnA_GG
Cuts at: 0 745 782 943 2292
Size: 745 37 161 1349
Fragments arranged by size:
1349 745 161 37
BtgI C'CryG_G
Cuts at: 0 1355 1964 2255 2292
Size: 1355 609 291 37
Fragments arranged by size:
1355 609 291 37
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 653 1727 2292
Size: 653 1074 565
Fragments arranged by size:
1074 653 565
BtsI GCAGTG_nn'
Cuts at: 0 828 1961 2292
Size: 828 1133 331
Fragments arranged by size:
1133 828 331
DrdI GACnn_nn'nnGTC
Cuts at: 0 412 844 2292
Size: 412 432 1448
Fragments arranged by size:
1448 432 412
EaeI y'GGCC_r
Cuts at: 0 594 797 1100 1176 1979 2258 2292
Size: 594 203 303 76 803 279 34
Fragments arranged by size:
803 594 303 279 203 76 34
EarI CTCTTCn'nnn_
Cuts at: 0 13 49 856 1036 1682 2106 2132 2292
Size: 13 36 807 180 646 424 26 160
Fragments arranged by size:
807 646 424 180 160 36 26 13
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 207 394 519 1267 1365 1893 2049 2227
Size: 207 187 125 748 98 528 156 178
Cuts at: 2227 2292
Size: 65
Fragments arranged by size:
748 528 207 187 178 156 125 98
65
EcoNI CCTnn'n_nnAGG
Cuts at: 0 946 1246 1726 2292
Size: 946 300 480 566
Fragments arranged by size:
946 566 480 300
EcoO109I rG'GnC_Cy
Cuts at: 0 388 778 866 867 1166 1437 1512 1534
Size: 388 390 88 1 299 271 75 22
Cuts at: 1534 1706 2292
Size: 172 586
Fragments arranged by size:
586 390 388 299 271 172 88 75
22 1
HaeII r_GCGC'y
Cuts at: 0 321 966 1918 2292
Size: 321 645 952 374
Fragments arranged by size:
952 645 374 321
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1904 1936 2003 2035 2292
Size: 1904 32 67 32 257
Fragments arranged by size:
1904 257 67 32 32
HincII GTy'rAC
Cuts at: 0 141 849 2007 2292
Size: 141 708 1158 285
Fragments arranged by size:
1158 708 285 141
KpnI G_GTAC'C
Cuts at: 0 1399 2292
Size: 1399 893
MscI TGG'CCA
Cuts at: 0 596 799 1981 2292
Size: 596 203 1182 311
Fragments arranged by size:
1182 596 311 203
MslI CAynn'nnrTG
Cuts at: 0 277 986 1072 1414 1652 1930 2044 2292
Size: 277 709 86 342 238 278 114 248
Fragments arranged by size:
709 342 278 277 248 238 114 86
MspA1I CmG'CkG
Cuts at: 0 297 996 1181 1702 2292
Size: 297 699 185 521 590
Fragments arranged by size:
699 590 521 297 185
NaeI GCC'GGC
Cuts at: 0 169 1308 1573 2292
Size: 169 1139 265 719
Fragments arranged by size:
1139 719 265 169
NgoMIV G'CCGG_C
Cuts at: 0 167 1306 1571 2292
Size: 167 1139 265 721
Fragments arranged by size:
1139 721 265 167
NspI r_CATG'y
Cuts at: 0 2049 2292
Size: 2049 243
PasI CC'CwG_GG
Cuts at: 0 774 855 1938 2292
Size: 774 81 1083 354
Fragments arranged by size:
1083 774 354 81
PfoI T'CCnGG_A
Cuts at: 0 260 2292
Size: 260 2032
PmlI CAC'GTG
Cuts at: 0 116 2292
Size: 116 2176
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1373 1405 2292
Size: 1373 32 887
Fragments arranged by size:
1373 887 32
PpuMI rG'GwC_Cy
Cuts at: 0 388 1437 1706 2292
Size: 388 1049 269 586
Fragments arranged by size:
1049 586 388 269
PspOMI G'GGCC_C
Cuts at: 0 777 866 1166 2292
Size: 777 89 300 1126
Fragments arranged by size:
1126 777 300 89
PstI C_TGCA'G
Cuts at: 0 1001 1668 2292
Size: 1001 667 624
Fragments arranged by size:
1001 667 624
PvuII CAG'CTG
Cuts at: 0 297 996 2292
Size: 297 699 1296
Fragments arranged by size:
1296 699 297
SalI G'TCGA_C
Cuts at: 0 2005 2292
Size: 2005 287
SanDI GG'GwC_CC
Cuts at: 0 1706 2292
Size: 1706 586
SapI GCTCTTCn'nnn_
Cuts at: 0 13 2292
Size: 13 2279
ScaI AGT'ACT
Cuts at: 0 160 1147 2292
Size: 160 987 1145
Fragments arranged by size:
1145 987 160
SexAI A'CCwGG_T
Cuts at: 0 1595 2292
Size: 1595 697
SfcI C'TryA_G
Cuts at: 0 997 1664 2028 2292
Size: 997 667 364 264
Fragments arranged by size:
997 667 364 264
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 2266 2292
Size: 2266 26
SmlI C'TyrA_G
Cuts at: 0 1390 1432 2292
Size: 1390 42 860
Fragments arranged by size:
1390 860 42
StuI AGG'CCT
Cuts at: 0 353 509 941 1021 1546 2292
Size: 353 156 432 80 525 746
Fragments arranged by size:
746 525 432 353 156 80
StyI C'CwwG_G
Cuts at: 0 2204 2292
Size: 2204 88
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 144 2024 2292
Size: 144 1880 268
Fragments arranged by size:
1880 268 144
TatI w'GTAC_w
Cuts at: 0 158 494 1145 2292
Size: 158 336 651 1147
Fragments arranged by size:
1147 651 336 158
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 42 74 1082 1114 2292
Size: 42 32 1008 32 1178
Fragments arranged by size:
1178 1008 42 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 841 2292
Size: 841 1451
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 910 2088 2292
Size: 910 1178 204
Fragments arranged by size:
1178 910 204
Enzymes that do cut:
AarI AccI Acc65I AfeI AleI AlfI AlwNI ApaI
ApoI AvaI BaeI BamHI BanI BanII BbsI BcgI
BclI BglI BlpI Bme1580I BmgBI BmrI BplI BpmI
BpuEI BsaI BsaAI BsaHI BsaXI BseRI BseYI BsiEI
BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspMI BsrDI BsrFI
BsrGI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI DrdI EaeI EarI Eco57MI EcoNI EcoO109I HaeII
Hin4I HincII KpnI MscI MslI MspA1I NaeI NgoMIV
NspI PasI PfoI PmlI PpiI PpuMI PspOMI PstI
PvuII SalI SanDI SapI ScaI SexAI SfcI SfiI
SmlI StuI StyI TaqII TatI TstI Tth111I XcmI
Enzymes that do not cut:
AatII AclI AcuI AflII AflIII AgeI AhdI AloI
ApaLI AscI AseI AsiSI AvrII BbeI BbvCI BciVI
BfrBI BglII BmtI Bpu10I BsaBI BsaWI BsgI BspEI
BspHI BsrBI BssHII BssSI BstBI BstZ17I ClaI CspCI
DraI DraIII EagI EciI EcoICRI EcoRI EcoRV FalI
FseI FspI FspAI HindIII HpaI KasI MfeI MluI
MmeI NarI NcoI NdeI NheI NotI NruI NsiI
PacI PciI PflMI PmeI PshAI PsiI PspXI PsrI
PvuI RsrII SacI SacII SbfI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI SwaI XbaI
XhoI XmaI XmnI ZraI