Restriction map for
pFN21AE2086
(Linear) (Six-base) MAPSORT of: KIEE2086.seq Check: 3359 from: 1 to: 2280
KIEE2086.orf
With 182 enzymes: SgfI *
July 15, 2010 18:54 ..
AatII G_ACGT'C
Cuts at: 0 389 2280
Size: 389 1891
AccI GT'mk_AC
Cuts at: 0 29 1877 2280
Size: 29 1848 403
Fragments arranged by size:
1848 403 29
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 559 1287 2280
Size: 559 728 993
Fragments arranged by size:
993 728 559
AgeI A'CCGG_T
Cuts at: 0 770 2280
Size: 770 1510
AhdI GACnn_n'nnGTC
Cuts at: 0 660 2280
Size: 660 1620
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1479 2280
Size: 1479 801
AlwNI CAG_nnn'CTG
Cuts at: 0 840 958 1121 2280
Size: 840 118 163 1159
Fragments arranged by size:
1159 840 163 118
ApaLI G'TGCA_C
Cuts at: 0 1831 2280
Size: 1831 449
ApoI r'AATT_y
Cuts at: 0 478 1519 2280
Size: 478 1041 761
Fragments arranged by size:
1041 761 478
AvaI C'yCGr_G
Cuts at: 0 55 893 1529 1799 2280
Size: 55 838 636 270 481
Fragments arranged by size:
838 636 481 270 55
BamHI G'GATC_C
Cuts at: 0 1667 2087 2280
Size: 1667 420 193
Fragments arranged by size:
1667 420 193
BanI G'GyrC_C
Cuts at: 0 133 501 554 566 773 1720 2280
Size: 133 368 53 12 207 947 560
Fragments arranged by size:
947 560 368 207 133 53 12
BanII G_rGCy'C
Cuts at: 0 23 83 331 1060 1259 2280
Size: 23 60 248 729 199 1021
Fragments arranged by size:
1021 729 248 199 60 23
BbeI G_GCGC'C
Cuts at: 0 558 2280
Size: 558 1722
BbsI GAAGACnn'nnnn_
Cuts at: 0 148 687 808 2280
Size: 148 539 121 1472
Fragments arranged by size:
1472 539 148 121
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 669 703 2280
Size: 669 34 1577
Fragments arranged by size:
1577 669 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 159 2280
Size: 159 2121
BclI T'GATC_A
Cuts at: 0 164 1478 2280
Size: 164 1314 802
Fragments arranged by size:
1314 802 164
BglI GCCn_nnn'nGGC
Cuts at: 0 682 913 1324 2280
Size: 682 231 411 956
Fragments arranged by size:
956 682 411 231
BlpI GC'TnA_GC
Cuts at: 0 2215 2270 2280
Size: 2215 55 10
Fragments arranged by size:
2215 55 10
Bme1580I G_kGCm'C
Cuts at: 0 506 571 1835 2280
Size: 506 65 1264 445
Fragments arranged by size:
1264 506 445 65
BmrI ACTGGGnnnn_n'
Cuts at: 0 1977 2280
Size: 1977 303
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 81 144 2280
Size: 81 63 2136
Fragments arranged by size:
2136 81 63
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 139 214 808 1030 1177 1594 1762 2242
Size: 139 75 594 222 147 417 168 480
Cuts at: 2242 2280
Size: 38
Fragments arranged by size:
594 480 417 222 168 147 139 75
38
Bpu10I CC'TnA_GC
Cuts at: 0 811 1072 2280
Size: 811 261 1208
Fragments arranged by size:
1208 811 261
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 204 1693 1882 2280
Size: 204 1489 189 398
Fragments arranged by size:
1489 398 204 189
BsaI GGTCTCn'nnnn_
Cuts at: 0 784 1519 1702 2280
Size: 784 735 183 578
Fragments arranged by size:
784 735 578 183
BsaAI yAC'GTr
Cuts at: 0 1017 2280
Size: 1017 1263
BsaHI Gr'CG_yC
Cuts at: 0 98 386 555 858 905 1316 1865 2280
Size: 98 288 169 303 47 411 549 415
Fragments arranged by size:
549 415 411 303 288 169 98 47
BsaWI w'CCGG_w
Cuts at: 0 770 1136 1550 2280
Size: 770 366 414 730
Fragments arranged by size:
770 730 414 366
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 2254 2280
Size: 2254 26
BseYI C'CCAG_C
Cuts at: 0 1085 2199 2280
Size: 1085 1114 81
Fragments arranged by size:
1114 1085 81
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1057 1069 1282 2280
Size: 1057 12 213 998
Fragments arranged by size:
1057 998 213 12
BsiEI CG_ry'CG
Cuts at: 0 977 1488 2280
Size: 977 511 792
Fragments arranged by size:
977 792 511
BsiHKAI G_wGCw'C
Cuts at: 0 83 331 812 1642 1835 2099 2280
Size: 83 248 481 830 193 264 181
Fragments arranged by size:
830 481 264 248 193 181 83
BsiWI C'GTAC_G
Cuts at: 0 38 2280
Size: 38 2242
BsmI GAATG_Cn'
Cuts at: 0 1892 2280
Size: 1892 388
BsmBI CGTCTCn'nnnn_
Cuts at: 0 892 2236 2280
Size: 892 1344 44
Fragments arranged by size:
1344 892 44
Bsp1286I G_dGCh'C
Cuts at: 0 23 83 331 506 571 812 1060 1259
Size: 23 60 248 175 65 241 248 199
Cuts at: 1259 1642 1835 2099 2280
Size: 383 193 264 181
Fragments arranged by size:
383 264 248 248 241 199 193 181
175 65 60 23
BspEI T'CCGG_A
Cuts at: 0 1550 2280
Size: 1550 730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1925 2280
Size: 1925 355
BsrBI CCG'CTC
Cuts at: 0 1076 1188 2280
Size: 1076 112 1092
Fragments arranged by size:
1092 1076 112
BsrDI GCAATG_nn'
Cuts at: 0 1280 1500 2280
Size: 1280 220 780
Fragments arranged by size:
1280 780 220
BsrFI r'CCGG_y
Cuts at: 0 770 854 2280
Size: 770 84 1426
Fragments arranged by size:
1426 770 84
BstAPI GCAn_nnn'nTGC
Cuts at: 0 840 1047 2280
Size: 840 207 1233
Fragments arranged by size:
1233 840 207
BstBI TT'CG_AA
Cuts at: 0 261 2280
Size: 261 2019
BstEII G'GTnAC_C
Cuts at: 0 198 2280
Size: 198 2082
BstXI CCAn_nnnn'nTGG
Cuts at: 0 612 672 1410 1452 2280
Size: 612 60 738 42 828
Fragments arranged by size:
828 738 612 60 42
BstYI r'GATC_y
Cuts at: 0 1406 1667 2087 2280
Size: 1406 261 420 193
Fragments arranged by size:
1406 420 261 193
Bsu36I CC'TnA_GG
Cuts at: 0 129 356 997 2280
Size: 129 227 641 1283
Fragments arranged by size:
1283 641 227 129
BtgI C'CryG_G
Cuts at: 0 489 1430 1488 1643 1773 2055 2126 2280
Size: 489 941 58 155 130 282 71 154
Fragments arranged by size:
941 489 282 155 154 130 71 58
BtsI GCAGTG_nn'
Cuts at: 0 459 959 2280
Size: 459 500 1321
Fragments arranged by size:
1321 500 459
EaeI y'GGCC_r
Cuts at: 0 914 974 1325 1485 1505 1770 2147 2280
Size: 914 60 351 160 20 265 377 133
Fragments arranged by size:
914 377 351 265 160 133 60 20
EagI C'GGCC_G
Cuts at: 0 974 1485 2280
Size: 974 511 795
Fragments arranged by size:
974 795 511
EarI CTCTTCn'nnn_
Cuts at: 0 248 2280
Size: 248 2032
EcoICRI GAG'CTC
Cuts at: 0 81 329 2280
Size: 81 248 1951
Fragments arranged by size:
1951 248 81
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 139 214 559 808 1030 1177 1287 1594
Size: 139 75 345 249 222 147 110 307
Cuts at: 1594 1762 2242 2280
Size: 168 480 38
Fragments arranged by size:
480 345 307 249 222 168 147 139
110 75 38
EcoO109I rG'GnC_Cy
Cuts at: 0 543 2280
Size: 543 1737
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1716 2280
Size: 1716 564
HaeII r_GCGC'y
Cuts at: 0 558 840 890 2280
Size: 558 282 50 1390
Fragments arranged by size:
1390 558 282 50
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 171 203 578 610 646 678 2280
Size: 171 32 375 32 36 32 1602
Fragments arranged by size:
1602 375 171 36 32 32 32
HincII GTy'rAC
Cuts at: 0 747 2280
Size: 747 1533
KasI G'GCGC_C
Cuts at: 0 554 2280
Size: 554 1726
MscI TGG'CCA
Cuts at: 0 916 1327 1507 2149 2280
Size: 916 411 180 642 131
Fragments arranged by size:
916 642 411 180 131
MslI CAynn'nnrTG
Cuts at: 0 494 605 2280
Size: 494 111 1675
Fragments arranged by size:
1675 494 111
MspA1I CmG'CkG
Cuts at: 0 192 514 1307 1389 1585 1775 2084 2199
Size: 192 322 793 82 196 190 309 115
Cuts at: 2199 2280
Size: 81
Fragments arranged by size:
793 322 309 196 192 190 115 82
81
NarI GG'CG_CC
Cuts at: 0 555 2280
Size: 555 1725
NcoI C'CATG_G
Cuts at: 0 489 1430 2280
Size: 489 941 850
Fragments arranged by size:
941 850 489
NspI r_CATG'y
Cuts at: 0 75 604 1045 2280
Size: 75 529 441 1235
Fragments arranged by size:
1235 529 441 75
PasI CC'CwG_GG
Cuts at: 0 546 2280
Size: 546 1734
PflMI CCAn_nnn'nTGG
Cuts at: 0 1632 1989 2280
Size: 1632 357 291
Fragments arranged by size:
1632 357 291
PfoI T'CCnGG_A
Cuts at: 0 116 635 1409 2090 2280
Size: 116 519 774 681 190
Fragments arranged by size:
774 681 519 190 116
PstI C_TGCA'G
Cuts at: 0 800 947 2252 2280
Size: 800 147 1305 28
Fragments arranged by size:
1305 800 147 28
PvuII CAG'CTG
Cuts at: 0 1307 2199 2280
Size: 1307 892 81
Fragments arranged by size:
1307 892 81
SacI G_AGCT'C
Cuts at: 0 83 331 2280
Size: 83 248 1949
Fragments arranged by size:
1949 248 83
SacII CC_GC'GG
Cuts at: 0 1776 2280
Size: 1776 504
SapI GCTCTTCn'nnn_
Cuts at: 0 248 2280
Size: 248 2032
ScaI AGT'ACT
Cuts at: 0 2155 2280
Size: 2155 125
SexAI A'CCwGG_T
Cuts at: 0 656 1499 2280
Size: 656 843 781
Fragments arranged by size:
843 781 656
SfcI C'TryA_G
Cuts at: 0 796 943 2248 2280
Size: 796 147 1305 32
Fragments arranged by size:
1305 796 147 32
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 682 2280
Size: 682 1598
SfoI GGC'GCC
Cuts at: 0 556 2280
Size: 556 1724
SmaI CCC'GGG
Cuts at: 0 895 1531 1801 2280
Size: 895 636 270 479
Fragments arranged by size:
895 636 479 270
SmlI C'TyrA_G
Cuts at: 0 219 1672 1897 2280
Size: 219 1453 225 383
Fragments arranged by size:
1453 383 225 219
SphI G_CATG'C
Cuts at: 0 604 2280
Size: 604 1676
StuI AGG'CCT
Cuts at: 0 1759 2280
Size: 1759 521
StyI C'CwwG_G
Cuts at: 0 101 489 665 1430 2280
Size: 101 388 176 765 850
Fragments arranged by size:
850 765 388 176 101
TatI w'GTAC_w
Cuts at: 0 1628 2153 2280
Size: 1628 525 127
Fragments arranged by size:
1628 525 127
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2165 2197 2280
Size: 2165 32 83
Fragments arranged by size:
2165 83 32
Tth111I GACn'n_nGTC
Cuts at: 0 721 1380 2280
Size: 721 659 900
Fragments arranged by size:
900 721 659
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1737 2280
Size: 1737 543
XmaI C'CCGG_G
Cuts at: 0 893 1529 1799 2280
Size: 893 636 270 481
Fragments arranged by size:
893 636 481 270
XmnI GAAnn'nnTTC
Cuts at: 0 257 2280
Size: 257 2023
ZraI GAC'GTC
Cuts at: 0 387 2280
Size: 387 1893
Enzymes that do cut:
AatII AccI AcuI AgeI AhdI AlfI AlwNI ApaLI
ApoI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI
BspMI BsrBI BsrDI BsrFI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtsI EaeI EagI EarI EcoICRI
Eco57MI EcoO109I FalI HaeII Hin4I HincII KasI MscI
MslI MspA1I NarI NcoI NspI PasI PflMI PfoI
PstI PvuII SacI SacII SapI ScaI SexAI SfcI
SfiI SfoI SmaI SmlI SphI StuI StyI TatI
TstI Tth111I XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AflIII AleI AloI
ApaI AscI AseI AsiSI AvrII BaeI BbvCI BfrBI
BglII BmgBI BmtI BsaBI BsaXI BspHI BsrGI BssHII
BssSI BstZ17I BtgZI ClaI CspCI DraI DraIII DrdI
EciI EcoNI EcoRI EcoRV FseI FspI FspAI HindIII
HpaI KpnI MfeI MluI MmeI NaeI NdeI NgoMIV
NheI NotI NruI NsiI PacI PciI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI
RsrII SalI SanDI SbfI SgfI SgrAI SnaBI SpeI
SrfI SspI SwaI TaqII XbaI XhoI