Restriction map for
pFN21AE2084
(Linear) (Six-base) MAPSORT of: KIEE2084.seq Check: 3579 from: 1 to: 2277
KIEE2084.orf
With 182 enzymes: SgfI *
December 5, 2014 12:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1675 2277
Size: 1675 602
AccI GT'mk_AC
Cuts at: 0 1118 1447 2277
Size: 1118 329 830
Fragments arranged by size:
1118 830 329
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 121 2277
Size: 121 2156
AlwNI CAG_nnn'CTG
Cuts at: 0 888 2277
Size: 888 1389
ApoI r'AATT_y
Cuts at: 0 341 416 655 729 813 1054 1284 1513
Size: 341 75 239 74 84 241 230 229
Cuts at: 1513 2061 2206 2277
Size: 548 145 71
Fragments arranged by size:
548 341 241 239 230 229 145 84
75 74 71
AvaI C'yCGr_G
Cuts at: 0 18 33 1886 2277
Size: 18 15 1853 391
Fragments arranged by size:
1853 391 18 15
BamHI G'GATC_C
Cuts at: 0 591 2277
Size: 591 1686
BanI G'GyrC_C
Cuts at: 0 37 1663 2277
Size: 37 1626 614
Fragments arranged by size:
1626 614 37
BbeI G_GCGC'C
Cuts at: 0 41 2277
Size: 41 2236
BbsI GAAGACnn'nnnn_
Cuts at: 0 1247 1871 2277
Size: 1247 624 406
Fragments arranged by size:
1247 624 406
BfrBI ATG'CAT
Cuts at: 0 1369 2277
Size: 1369 908
BglII A'GATC_T
Cuts at: 0 2196 2277
Size: 2196 81
BlpI GC'TnA_GC
Cuts at: 0 1903 2277
Size: 1903 374
BmrI ACTGGGnnnn_n'
Cuts at: 0 861 2277
Size: 861 1416
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 879 2277
Size: 879 1398
Bpu10I CC'TnA_GC
Cuts at: 0 704 1698 2277
Size: 704 994 579
Fragments arranged by size:
994 704 579
BsaBI GATnn'nnATC
Cuts at: 0 545 2277
Size: 545 1732
BsaHI Gr'CG_yC
Cuts at: 0 38 2277
Size: 38 2239
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1917 1947 2277
Size: 1917 30 330
Fragments arranged by size:
1917 330 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 33 36 39 139 601 2277
Size: 33 3 3 100 462 1676
Fragments arranged by size:
1676 462 100 33 3 3
BsiHKAI G_wGCw'C
Cuts at: 0 25 2277
Size: 25 2252
BsmI GAATG_Cn'
Cuts at: 0 484 522 1369 2277
Size: 484 38 847 908
Fragments arranged by size:
908 847 484 38
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1438 2277
Size: 1438 839
Bsp1286I G_dGCh'C
Cuts at: 0 25 2277
Size: 25 2252
BspHI T'CATG_A
Cuts at: 0 1280 1338 1860 2277
Size: 1280 58 522 417
Fragments arranged by size:
1280 522 417 58
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1675 2277
Size: 1675 602
BsrDI GCAATG_nn'
Cuts at: 0 694 1644 1832 2277
Size: 694 950 188 445
Fragments arranged by size:
950 694 445 188
BssSI C'ACGA_G
Cuts at: 0 734 2277
Size: 734 1543
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1306 2277
Size: 1306 971
BstEII G'GTnAC_C
Cuts at: 0 919 2277
Size: 919 1358
BstXI CCAn_nnnn'nTGG
Cuts at: 0 469 1818 2277
Size: 469 1349 459
Fragments arranged by size:
1349 469 459
BstYI r'GATC_y
Cuts at: 0 591 2196 2277
Size: 591 1605 81
Fragments arranged by size:
1605 591 81
BstZ17I GTA'TAC
Cuts at: 0 1448 2277
Size: 1448 829
ClaI AT'CG_AT
Cuts at: 0 549 2277
Size: 549 1728
DraI TTT'AAA
Cuts at: 0 629 2048 2277
Size: 629 1419 229
Fragments arranged by size:
1419 629 229
DraIII CAC_nnn'GTG
Cuts at: 0 488 2277
Size: 488 1789
EaeI y'GGCC_r
Cuts at: 0 557 979 2277
Size: 557 422 1298
Fragments arranged by size:
1298 557 422
EarI CTCTTCn'nnn_
Cuts at: 0 78 2068 2277
Size: 78 1990 209
Fragments arranged by size:
1990 209 78
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 121 2277
Size: 121 2156
EcoRI G'AATT_C
Cuts at: 0 341 655 729 1284 2277
Size: 341 314 74 555 993
Fragments arranged by size:
993 555 341 314 74
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 168 2277
Size: 168 2109
HaeII r_GCGC'y
Cuts at: 0 41 100 2277
Size: 41 59 2177
Fragments arranged by size:
2177 59 41
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1842 1874 1992 2024 2277
Size: 1842 32 118 32 253
Fragments arranged by size:
1842 253 118 32 32
HincII GTy'rAC
Cuts at: 0 581 2277
Size: 581 1696
HindIII A'AGCT_T
Cuts at: 0 145 1839 2277
Size: 145 1694 438
Fragments arranged by size:
1694 438 145
HpaI GTT'AAC
Cuts at: 0 581 2277
Size: 581 1696
KasI G'GCGC_C
Cuts at: 0 37 2277
Size: 37 2240
MfeI C'AATT_G
Cuts at: 0 1553 2277
Size: 1553 724
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1217 2277
Size: 1217 1060
MscI TGG'CCA
Cuts at: 0 559 981 2277
Size: 559 422 1296
Fragments arranged by size:
1296 559 422
NarI GG'CG_CC
Cuts at: 0 38 2277
Size: 38 2239
NsiI A_TGCA'T
Cuts at: 0 1371 2277
Size: 1371 906
NspI r_CATG'y
Cuts at: 0 1708 2277
Size: 1708 569
PshAI GACnn'nnGTC
Cuts at: 0 912 2277
Size: 912 1365
PspXI vC'TCGA_Gb
Cuts at: 0 1886 2277
Size: 1886 391
PstI C_TGCA'G
Cuts at: 0 302 1751 2277
Size: 302 1449 526
Fragments arranged by size:
1449 526 302
SfcI C'TryA_G
Cuts at: 0 298 1747 2277
Size: 298 1449 530
Fragments arranged by size:
1449 530 298
SfoI GGC'GCC
Cuts at: 0 39 2277
Size: 39 2238
SmlI C'TyrA_G
Cuts at: 0 1886 2277
Size: 1886 391
TatI w'GTAC_w
Cuts at: 0 2152 2277
Size: 2152 125
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 720 752 2277
Size: 720 32 1525
Fragments arranged by size:
1525 720 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1461 1657 1818 2277
Size: 1461 196 161 459
Fragments arranged by size:
1461 459 196 161
XhoI C'TCGA_G
Cuts at: 0 1886 2277
Size: 1886 391
XmnI GAAnn'nnTTC
Cuts at: 0 148 341 749 2277
Size: 148 193 408 1528
Fragments arranged by size:
1528 408 193 148
Enzymes that do cut:
AarI AccI AcuI AlwNI ApoI AvaI BamHI BanI
BbeI BbsI BfrBI BglII BlpI BmrI BplI Bpu10I
BsaBI BsaHI BsaXI BseRI BsiHKAI BsmI BsmBI Bsp1286I
BspHI BspMI BsrDI BssSI BstAPI BstEII BstXI BstYI
BstZ17I ClaI DraI DraIII EaeI EarI Eco57MI EcoRI
FalI HaeII Hin4I HincII HindIII HpaI KasI MfeI
MmeI MscI NarI NsiI NspI PshAI PspXI PstI
SfcI SfoI SmlI TatI TstI XcmI XhoI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI
AvrII BaeI BanII BbvCI BcgI BciVI BclI BglI
Bme1580I BmgBI BmtI BpmI BpuEI BsaI BsaAI BsaWI
BseYI BsgI BsiEI BsiWI BspEI BsrBI BsrFI BsrGI
BssHII BstBI Bsu36I BtgI BtgZI BtsI CspCI DrdI
EagI EciI EcoICRI EcoNI EcoO109I EcoRV FseI FspI
FspAI KpnI MluI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NruI PacI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PsiI PspOMI PsrI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI StuI StyI SwaI TaqII
Tth111I XbaI XmaI ZraI