Restriction map for
pFN21AE2081
(Linear) (Six-base) MAPSORT of: KIEE2081.seq Check: 8616 from: 1 to: 2262
KIEE2081.orf
With 182 enzymes: SgfI *
July 15, 2010 18:54 ..
AccI GT'mk_AC
Cuts at: 0 808 2262
Size: 808 1454
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 219 689 2262
Size: 219 470 1573
Fragments arranged by size:
1573 470 219
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1738 2262
Size: 1738 524
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 28 2262
Size: 28 2234
ApoI r'AATT_y
Cuts at: 0 319 708 1320 1968 2215 2262
Size: 319 389 612 648 247 47
Fragments arranged by size:
648 612 389 319 247 47
BanII G_rGCy'C
Cuts at: 0 1107 1891 1954 2262
Size: 1107 784 63 308
Fragments arranged by size:
1107 784 308 63
BbsI GAAGACnn'nnnn_
Cuts at: 0 293 1340 2255 2262
Size: 293 1047 915 7
Fragments arranged by size:
1047 915 293 7
BciVI GTATCCnnnnn_n'
Cuts at: 0 345 503 2262
Size: 345 158 1759
Fragments arranged by size:
1759 345 158
BclI T'GATC_A
Cuts at: 0 1590 2007 2220 2262
Size: 1590 417 213 42
Fragments arranged by size:
1590 417 213 42
BglII A'GATC_T
Cuts at: 0 982 2262
Size: 982 1280
Bme1580I G_kGCm'C
Cuts at: 0 100 2262
Size: 100 2162
BmrI ACTGGGnnnn_n'
Cuts at: 0 798 2262
Size: 798 1464
BmtI G_CTAG'C
Cuts at: 0 488 2262
Size: 488 1774
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 603 2262
Size: 603 1659
BsaBI GATnn'nnATC
Cuts at: 0 407 1041 2051 2262
Size: 407 634 1010 211
Fragments arranged by size:
1010 634 407 211
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1530 1560 2262
Size: 1530 30 702
Fragments arranged by size:
1530 702 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 269 383 582 585 1064 1878 1887 2262
Size: 269 114 199 3 479 814 9 375
Fragments arranged by size:
814 479 375 269 199 114 9 3
BseYI C'CCAG_C
Cuts at: 0 944 2262
Size: 944 1318
BsiHKAI G_wGCw'C
Cuts at: 0 1107 1891 1954 2262
Size: 1107 784 63 308
Fragments arranged by size:
1107 784 308 63
BsmBI CGTCTCn'nnnn_
Cuts at: 0 957 2262
Size: 957 1305
Bsp1286I G_dGCh'C
Cuts at: 0 100 1107 1891 1954 2262
Size: 100 1007 784 63 308
Fragments arranged by size:
1007 784 308 100 63
BspHI T'CATG_A
Cuts at: 0 1479 2223 2262
Size: 1479 744 39
Fragments arranged by size:
1479 744 39
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 66 2262
Size: 66 2196
BsrDI GCAATG_nn'
Cuts at: 0 38 2262
Size: 38 2224
BsrGI T'GTAC_A
Cuts at: 0 130 2196 2262
Size: 130 2066 66
Fragments arranged by size:
2066 130 66
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1095 2262
Size: 1095 1167
BstYI r'GATC_y
Cuts at: 0 827 982 1036 2143 2262
Size: 827 155 54 1107 119
Fragments arranged by size:
1107 827 155 119 54
EaeI y'GGCC_r
Cuts at: 0 335 2262
Size: 335 1927
EarI CTCTTCn'nnn_
Cuts at: 0 60 642 665 751 818 999 1713 1794
Size: 60 582 23 86 67 181 714 81
Cuts at: 1794 2262
Size: 468
Fragments arranged by size:
714 582 468 181 86 81 67 60
23
EcoICRI GAG'CTC
Cuts at: 0 1105 1889 1952 2262
Size: 1105 784 63 310
Fragments arranged by size:
1105 784 310 63
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 219 689 2262
Size: 219 470 1573
Fragments arranged by size:
1573 470 219
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 416 448 616 648 852 884 1273 1305
Size: 416 32 168 32 204 32 389 32
Cuts at: 1305 2212 2244 2262
Size: 907 32 18
Fragments arranged by size:
907 416 389 204 168 32 32 32
32 32 18
MscI TGG'CCA
Cuts at: 0 337 2262
Size: 337 1925
MslI CAynn'nnrTG
Cuts at: 0 401 1229 1484 2198 2262
Size: 401 828 255 714 64
Fragments arranged by size:
828 714 401 255 64
NheI G'CTAG_C
Cuts at: 0 484 2262
Size: 484 1778
NspI r_CATG'y
Cuts at: 0 110 2262
Size: 110 2152
PfoI T'CCnGG_A
Cuts at: 0 1630 2262
Size: 1630 632
PsiI TTA'TAA
Cuts at: 0 179 1673 2262
Size: 179 1494 589
Fragments arranged by size:
1494 589 179
SacI G_AGCT'C
Cuts at: 0 1107 1891 1954 2262
Size: 1107 784 63 308
Fragments arranged by size:
1107 784 308 63
SmlI C'TyrA_G
Cuts at: 0 618 2262
Size: 618 1644
StyI C'CwwG_G
Cuts at: 0 1012 2262
Size: 1012 1250
TatI w'GTAC_w
Cuts at: 0 130 1991 2196 2262
Size: 130 1861 205 66
Fragments arranged by size:
1861 205 130 66
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 910 942 2262
Size: 910 32 1320
Fragments arranged by size:
1320 910 32
XmnI GAAnn'nnTTC
Cuts at: 0 1029 1320 2262
Size: 1029 291 942
Fragments arranged by size:
1029 942 291
Enzymes that do cut:
AccI AcuI AlfI AloI ApoI BanII BbsI BciVI
BclI BglII Bme1580I BmrI BmtI BpuEI BsaBI BsaXI
BseRI BseYI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrDI
BsrGI BstAPI BstYI EaeI EarI EcoICRI Eco57MI Hin4I
MscI MslI NheI NspI PfoI PsiI SacI SmlI
StyI TatI TstI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlwNI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BbeI BbvCI BcgI
BfrBI BglI BlpI BmgBI BplI BpmI Bpu10I BsaI
BsaAI BsaHI BsaWI BsgI BsiEI BsiWI BsmI BspEI
BsrBI BsrFI BssHII BssSI BstBI BstEII BstXI BstZ17I
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EagI EciI EcoNI EcoO109I EcoRI EcoRV FalI
FseI FspI FspAI HaeII HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MspA1I NaeI NarI NcoI
NdeI NgoMIV NotI NruI NsiI PacI PasI PciI
PflMI PmeI PmlI PpiI PpuMI PshAI PspOMI PspXI
PsrI PstI PvuI PvuII RsrII SacII SalI SanDI
SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI
SwaI TaqII Tth111I XbaI XcmI XhoI XmaI ZraI