Restriction map for
pFN21AE2079
(Linear) (Six-base) MAPSORT of: KIEE2079.seq Check: 6047 from: 1 to: 2256
KIEE2079.orf
With 182 enzymes: SgfI *
July 15, 2010 18:54 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 837 1714 2256
Size: 837 877 542
Fragments arranged by size:
877 837 542
AfeI AGC'GCT
Cuts at: 0 745 2256
Size: 745 1511
AflII C'TTAA_G
Cuts at: 0 1885 2256
Size: 1885 371
AflIII A'CryG_T
Cuts at: 0 1400 2256
Size: 1400 856
AleI CACnn'nnGTG
Cuts at: 0 186 2256
Size: 186 2070
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 274 1536 2256
Size: 274 1262 720
Fragments arranged by size:
1262 720 274
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 919 951 2256
Size: 919 32 1305
Fragments arranged by size:
1305 919 32
AlwNI CAG_nnn'CTG
Cuts at: 0 162 274 1796 2226 2256
Size: 162 112 1522 430 30
Fragments arranged by size:
1522 430 162 112 30
ApaI G_GGCC'C
Cuts at: 0 7 1485 2151 2256
Size: 7 1478 666 105
Fragments arranged by size:
1478 666 105 7
ApoI r'AATT_y
Cuts at: 0 1594 2241 2256
Size: 1594 647 15
Fragments arranged by size:
1594 647 15
AvaI C'yCGr_G
Cuts at: 0 90 938 2256
Size: 90 848 1318
Fragments arranged by size:
1318 848 90
BanI G'GyrC_C
Cuts at: 0 97 2256
Size: 97 2159
BanII G_rGCy'C
Cuts at: 0 7 126 376 605 905 1485 2151 2256
Size: 7 119 250 229 300 580 666 105
Fragments arranged by size:
666 580 300 250 229 119 105 7
BbsI GAAGACnn'nnnn_
Cuts at: 0 548 712 2256
Size: 548 164 1544
Fragments arranged by size:
1544 548 164
BbvCI CC'TCA_GC
Cuts at: 0 109 2215 2256
Size: 109 2106 41
Fragments arranged by size:
2106 109 41
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 525 559 2256
Size: 525 34 1697
Fragments arranged by size:
1697 525 34
BclI T'GATC_A
Cuts at: 0 956 2256
Size: 956 1300
BglI GCCn_nnn'nGGC
Cuts at: 0 158 401 1768 2256
Size: 158 243 1367 488
Fragments arranged by size:
1367 488 243 158
Bme1580I G_kGCm'C
Cuts at: 0 7 664 1485 2151 2256
Size: 7 657 821 666 105
Fragments arranged by size:
821 666 657 105 7
BmgBI CAC'GTC
Cuts at: 0 645 2256
Size: 645 1611
BmrI ACTGGGnnnn_n'
Cuts at: 0 481 1013 1575 2256
Size: 481 532 562 681
Fragments arranged by size:
681 562 532 481
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 846 1941 2256
Size: 846 1095 315
Fragments arranged by size:
1095 846 315
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 609 1278 1684 2256
Size: 609 669 406 572
Fragments arranged by size:
669 609 572 406
Bpu10I CC'TnA_GC
Cuts at: 0 109 2215 2256
Size: 109 2106 41
Fragments arranged by size:
2106 109 41
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1769 2074 2256
Size: 1769 305 182
Fragments arranged by size:
1769 305 182
BsaI GGTCTCn'nnnn_
Cuts at: 0 236 1462 1562 1773 2256
Size: 236 1226 100 211 483
Fragments arranged by size:
1226 483 236 211 100
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 553 583 919 949 1528 1558 2119 2149
Size: 553 30 336 30 579 30 561 30
Cuts at: 2149 2256
Size: 107
Fragments arranged by size:
579 561 553 336 107 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 522 1057 2256
Size: 522 535 1199
Fragments arranged by size:
1199 535 522
BseYI C'CCAG_C
Cuts at: 0 119 174 309 2093 2256
Size: 119 55 135 1784 163
Fragments arranged by size:
1784 163 135 119 55
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1318 1734 2256
Size: 1318 416 522
Fragments arranged by size:
1318 522 416
BsiHKAI G_wGCw'C
Cuts at: 0 548 2256
Size: 548 1708
Bsp1286I G_dGCh'C
Cuts at: 0 7 126 376 548 605 664 905 1485
Size: 7 119 250 172 57 59 241 580
Cuts at: 1485 2151 2256
Size: 666 105
Fragments arranged by size:
666 580 250 241 172 119 105 59
57 7
BspHI T'CATG_A
Cuts at: 0 990 1043 2256
Size: 990 53 1213
Fragments arranged by size:
1213 990 53
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 776 1480 2256
Size: 776 704 776
Fragments arranged by size:
776 776 704
BsrBI CCG'CTC
Cuts at: 0 564 2058 2256
Size: 564 1494 198
Fragments arranged by size:
1494 564 198
BsrDI GCAATG_nn'
Cuts at: 0 339 1518 2256
Size: 339 1179 738
Fragments arranged by size:
1179 738 339
BssSI C'ACGA_G
Cuts at: 0 2174 2256
Size: 2174 82
BstXI CCAn_nnnn'nTGG
Cuts at: 0 682 748 1236 2256
Size: 682 66 488 1020
Fragments arranged by size:
1020 682 488 66
BstYI r'GATC_y
Cuts at: 0 848 1181 2256
Size: 848 333 1075
Fragments arranged by size:
1075 848 333
Bsu36I CC'TnA_GG
Cuts at: 0 638 2256
Size: 638 1618
BtgI C'CryG_G
Cuts at: 0 114 304 323 2256
Size: 114 190 19 1933
Fragments arranged by size:
1933 190 114 19
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1544 2256
Size: 1544 712
BtsI GCAGTG_nn'
Cuts at: 0 447 485 2001 2256
Size: 447 38 1516 255
Fragments arranged by size:
1516 447 255 38
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1125 1160 2256
Size: 1125 35 1096
Fragments arranged by size:
1125 1096 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 1501 2256
Size: 1501 755
EaeI y'GGCC_r
Cuts at: 0 444 2256
Size: 444 1812
EarI CTCTTCn'nnn_
Cuts at: 0 1195 2200 2256
Size: 1195 1005 56
Fragments arranged by size:
1195 1005 56
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 609 837 1278 1684 1714 2256
Size: 609 228 441 406 30 542
Fragments arranged by size:
609 542 441 406 228 30
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2214 2256
Size: 2214 42
EcoO109I rG'GnC_Cy
Cuts at: 0 159 314 1481 1482 1503 2000 2147 2148
Size: 159 155 1167 1 21 497 147 1
Cuts at: 2148 2163 2256
Size: 15 93
Fragments arranged by size:
1167 497 159 155 147 93 21 15
1 1
EcoRI G'AATT_C
Cuts at: 0 1594 2256
Size: 1594 662
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1615 2256
Size: 1615 641
HaeII r_GCGC'y
Cuts at: 0 747 830 2256
Size: 747 83 1426
Fragments arranged by size:
1426 747 83
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1094 1117 1126 1149 1264 1296 1500 1532
Size: 1094 23 9 23 115 32 204 32
Cuts at: 1532 1561 1593 2082 2114 2119 2151 2256
Size: 29 32 489 32 5 32 105
Fragments arranged by size:
1094 489 204 115 105 32 32 32
32 32 29 23 23 9 5
HincII GTy'rAC
Cuts at: 0 1167 2256
Size: 1167 1089
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 776 1125 1517 2256
Size: 776 349 392 739
Fragments arranged by size:
776 739 392 349
MscI TGG'CCA
Cuts at: 0 446 2256
Size: 446 1810
MslI CAynn'nnrTG
Cuts at: 0 186 995 1234 1811 2256
Size: 186 809 239 577 445
Fragments arranged by size:
809 577 445 239 186
MspA1I CmG'CkG
Cuts at: 0 200 424 1162 2256
Size: 200 224 738 1094
Fragments arranged by size:
1094 738 224 200
NcoI C'CATG_G
Cuts at: 0 114 2256
Size: 114 2142
NdeI CA'TA_TG
Cuts at: 0 1352 2256
Size: 1352 904
NspI r_CATG'y
Cuts at: 0 408 681 1108 1306 1404 1444 2205 2256
Size: 408 273 427 198 98 40 761 51
Fragments arranged by size:
761 427 408 273 198 98 51 40
PasI CC'CwG_GG
Cuts at: 0 156 657 1624 2256
Size: 156 501 967 632
Fragments arranged by size:
967 632 501 156
PciI A'CATG_T
Cuts at: 0 1400 2256
Size: 1400 856
PfoI T'CCnGG_A
Cuts at: 0 773 1661 2256
Size: 773 888 595
Fragments arranged by size:
888 773 595
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 919 951 2256
Size: 919 32 1305
Fragments arranged by size:
1305 919 32
PpuMI rG'GwC_Cy
Cuts at: 0 314 1503 2000 2163 2256
Size: 314 1189 497 163 93
Fragments arranged by size:
1189 497 314 163 93
PshAI GACnn'nnGTC
Cuts at: 0 1960 2256
Size: 1960 296
PspOMI G'GGCC_C
Cuts at: 0 3 1481 2147 2256
Size: 3 1478 666 109
Fragments arranged by size:
1478 666 109 3
PstI C_TGCA'G
Cuts at: 0 1319 2256
Size: 1319 937
PvuII CAG'CTG
Cuts at: 0 424 1162 2256
Size: 424 738 1094
Fragments arranged by size:
1094 738 424
SanDI GG'GwC_CC
Cuts at: 0 2000 2256
Size: 2000 256
SexAI A'CCwGG_T
Cuts at: 0 1337 2256
Size: 1337 919
SfcI C'TryA_G
Cuts at: 0 1315 1797 2256
Size: 1315 482 459
Fragments arranged by size:
1315 482 459
SmaI CCC'GGG
Cuts at: 0 940 2256
Size: 940 1316
SmlI C'TyrA_G
Cuts at: 0 1784 1885 2053 2256
Size: 1784 101 168 203
Fragments arranged by size:
1784 203 168 101
SphI G_CATG'C
Cuts at: 0 1306 2205 2256
Size: 1306 899 51
Fragments arranged by size:
1306 899 51
StyI C'CwwG_G
Cuts at: 0 114 2115 2256
Size: 114 2001 141
Fragments arranged by size:
2001 141 114
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 316 715 1132 1738 2256
Size: 316 399 417 606 518
Fragments arranged by size:
606 518 417 399 316
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 553 585 1896 1928 2256
Size: 553 32 1311 32 328
Fragments arranged by size:
1311 553 328 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 514 2256
Size: 514 1742
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1389 2256
Size: 1389 867
XmaI C'CCGG_G
Cuts at: 0 938 2256
Size: 938 1318
Enzymes that do cut:
AcuI AfeI AflII AflIII AleI AlfI AloI AlwNI
ApaI ApoI AvaI BanI BanII BbsI BbvCI BcgI
BclI BglI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I
BspHI BspMI BsrBI BsrDI BssSI BstXI BstYI Bsu36I
BtgI BtgZI BtsI CspCI DrdI EaeI EarI Eco57MI
EcoNI EcoO109I EcoRI FalI HaeII Hin4I HincII MmeI
MscI MslI MspA1I NcoI NdeI NspI PasI PciI
PfoI PpiI PpuMI PshAI PspOMI PstI PvuII SanDI
SexAI SfcI SmaI SmlI SphI StyI TaqII TstI
Tth111I XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AgeI AhdI ApaLI
AscI AseI AsiSI AvrII BaeI BamHI BbeI BciVI
BfrBI BglII BlpI BmtI BsaAI BsaBI BsaHI BsaWI
BsiEI BsiWI BsmI BsmBI BspEI BsrFI BsrGI BssHII
BstAPI BstBI BstEII BstZ17I ClaI DraI DraIII EagI
EciI EcoICRI EcoRV FseI FspI FspAI HindIII HpaI
KasI KpnI MfeI MluI NaeI NarI NgoMIV NheI
NotI NruI NsiI PacI PflMI PmeI PmlI PsiI
PspXI PsrI PvuI RsrII SacI SacII SalI SapI
SbfI ScaI SfiI SfoI SgfI SgrAI SnaBI SpeI
SrfI SspI StuI SwaI TatI XbaI XhoI XmnI
ZraI