Restriction map for
pFN21AE2077
(Linear) (Six-base) MAPSORT of: KIEE2077.seq Check: 5569 from: 1 to: 2235
KIEE2077.orf
With 182 enzymes: SgfI *
July 15, 2010 18:54 ..
AatII G_ACGT'C
Cuts at: 0 1289 2235
Size: 1289 946
AccI GT'mk_AC
Cuts at: 0 1429 2235
Size: 1429 806
AclI AA'CG_TT
Cuts at: 0 1323 2235
Size: 1323 912
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 352 448 1216 1379 1780 2235
Size: 352 96 768 163 401 455
Fragments arranged by size:
768 455 401 352 163 96
AfeI AGC'GCT
Cuts at: 0 1449 2235
Size: 1449 786
AlwNI CAG_nnn'CTG
Cuts at: 0 942 2235
Size: 942 1293
ApoI r'AATT_y
Cuts at: 0 589 614 672 740 1045 1948 2235
Size: 589 25 58 68 305 903 287
Fragments arranged by size:
903 589 305 287 68 58 25
BbsI GAAGACnn'nnnn_
Cuts at: 0 527 1241 2235
Size: 527 714 994
Fragments arranged by size:
994 714 527
BclI T'GATC_A
Cuts at: 0 1080 1370 2235
Size: 1080 290 865
Fragments arranged by size:
1080 865 290
BmrI ACTGGGnnnn_n'
Cuts at: 0 279 324 2132 2235
Size: 279 45 1808 103
Fragments arranged by size:
1808 279 103 45
BmtI G_CTAG'C
Cuts at: 0 427 2235
Size: 427 1808
BsaHI Gr'CG_yC
Cuts at: 0 1286 2206 2235
Size: 1286 920 29
Fragments arranged by size:
1286 920 29
BsaWI w'CCGG_w
Cuts at: 0 2024 2235
Size: 2024 211
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1548 1578 2235
Size: 1548 30 657
Fragments arranged by size:
1548 657 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 311 2030 2235
Size: 311 1719 205
Fragments arranged by size:
1719 311 205
BsmI GAATG_Cn'
Cuts at: 0 488 1316 2235
Size: 488 828 919
Fragments arranged by size:
919 828 488
BspHI T'CATG_A
Cuts at: 0 789 2060 2235
Size: 789 1271 175
Fragments arranged by size:
1271 789 175
BsrGI T'GTAC_A
Cuts at: 0 1608 1678 2235
Size: 1608 70 557
Fragments arranged by size:
1608 557 70
BssSI C'ACGA_G
Cuts at: 0 1245 2235
Size: 1245 990
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1350 2235
Size: 1350 885
BstZ17I GTA'TAC
Cuts at: 0 1430 2235
Size: 1430 805
BtgI C'CryG_G
Cuts at: 0 68 543 1724 2235
Size: 68 475 1181 511
Fragments arranged by size:
1181 511 475 68
DraI TTT'AAA
Cuts at: 0 1953 2235
Size: 1953 282
DrdI GACnn_nn'nnGTC
Cuts at: 0 1285 2235
Size: 1285 950
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 352 448 1216 1379 1780 2235
Size: 352 96 768 163 401 455
Fragments arranged by size:
768 455 401 352 163 96
EcoNI CCTnn'n_nnAGG
Cuts at: 0 991 2235
Size: 991 1244
EcoO109I rG'GnC_Cy
Cuts at: 0 966 1110 2235
Size: 966 144 1125
Fragments arranged by size:
1125 966 144
EcoRV GAT'ATC
Cuts at: 0 123 2235
Size: 123 2112
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 382 1305 2235
Size: 382 923 930
Fragments arranged by size:
930 923 382
HaeII r_GCGC'y
Cuts at: 0 1451 2235
Size: 1451 784
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 100 132 1888 1920 2235
Size: 100 32 1756 32 315
Fragments arranged by size:
1756 315 100 32 32
HincII GTy'rAC
Cuts at: 0 799 2235
Size: 799 1436
HindIII A'AGCT_T
Cuts at: 0 665 2235
Size: 665 1570
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 59 1082 2235
Size: 59 1023 1153
Fragments arranged by size:
1153 1023 59
MslI CAynn'nnrTG
Cuts at: 0 956 1258 1348 1643 1810 2235
Size: 956 302 90 295 167 425
Fragments arranged by size:
956 425 302 295 167 90
NcoI C'CATG_G
Cuts at: 0 543 1724 2235
Size: 543 1181 511
Fragments arranged by size:
1181 543 511
NdeI CA'TA_TG
Cuts at: 0 929 1716 2235
Size: 929 787 519
Fragments arranged by size:
929 787 519
NheI G'CTAG_C
Cuts at: 0 423 2235
Size: 423 1812
NspI r_CATG'y
Cuts at: 0 838 1005 2235
Size: 838 167 1230
Fragments arranged by size:
1230 838 167
PsiI TTA'TAA
Cuts at: 0 1787 2235
Size: 1787 448
ScaI AGT'ACT
Cuts at: 0 248 1180 2235
Size: 248 932 1055
Fragments arranged by size:
1055 932 248
SfcI C'TryA_G
Cuts at: 0 1534 2048 2235
Size: 1534 514 187
Fragments arranged by size:
1534 514 187
StyI C'CwwG_G
Cuts at: 0 156 543 1724 2235
Size: 156 387 1181 511
Fragments arranged by size:
1181 511 387 156
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 343 2235
Size: 343 1892
TatI w'GTAC_w
Cuts at: 0 246 1178 1608 1678 2235
Size: 246 932 430 70 557
Fragments arranged by size:
932 557 430 246 70
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1642 2235
Size: 1642 593
XmnI GAAnn'nnTTC
Cuts at: 0 461 589 713 740 2235
Size: 461 128 124 27 1495
Fragments arranged by size:
1495 461 128 124 27
ZraI GAC'GTC
Cuts at: 0 1287 2235
Size: 1287 948
Enzymes that do cut:
AatII AccI AclI AcuI AfeI AlwNI ApoI BbsI
BclI BmrI BmtI BsaHI BsaWI BsaXI BsgI BsmI
BspHI BsrGI BssSI BstXI BstZ17I BtgI DraI DrdI
Eco57MI EcoNI EcoO109I EcoRV FalI HaeII Hin4I HincII
HindIII MmeI MslI NcoI NdeI NheI NspI PsiI
ScaI SfcI StyI TaqII TatI XcmI XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AflII AflIII AgeI AhdI AleI AlfI
AloI ApaI ApaLI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BanI BanII BbeI BbvCI BcgI BciVI
BfrBI BglI BglII BlpI Bme1580I BmgBI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BseRI BseYI BsiEI
BsiHKAI BsiWI BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI
BsrFI BssHII BstAPI BstBI BstEII BstYI Bsu36I BtgZI
BtsI ClaI CspCI DraIII EaeI EagI EarI EciI
EcoICRI EcoRI FseI FspI FspAI HpaI KasI KpnI
MfeI MluI MscI MspA1I NaeI NarI NgoMIV NotI
NruI NsiI PacI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PspOMI PspXI PsrI PstI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI SwaI TstI
Tth111I XbaI XhoI XmaI