Restriction map for
pFN21AE2075
(Linear) (Six-base) MAPSORT of: KIEE2075.seq Check: 4833 from: 1 to: 2217
KIEE2075.orf
With 182 enzymes: SgfI *
July 15, 2010 18:54 ..
AccI GT'mk_AC
Cuts at: 0 425 656 750 2217
Size: 425 231 94 1467
Fragments arranged by size:
1467 425 231 94
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1924 2217
Size: 1924 293
AflII C'TTAA_G
Cuts at: 0 1321 2217
Size: 1321 896
AflIII A'CryG_T
Cuts at: 0 1000 1870 2217
Size: 1000 870 347
Fragments arranged by size:
1000 870 347
AhdI GACnn_n'nnGTC
Cuts at: 0 510 2217
Size: 510 1707
AlwNI CAG_nnn'CTG
Cuts at: 0 418 2217
Size: 418 1799
ApaLI G'TGCA_C
Cuts at: 0 282 1146 2217
Size: 282 864 1071
Fragments arranged by size:
1071 864 282
ApoI r'AATT_y
Cuts at: 0 487 860 1477 2217
Size: 487 373 617 740
Fragments arranged by size:
740 617 487 373
AseI AT'TA_AT
Cuts at: 0 916 1780 2217
Size: 916 864 437
Fragments arranged by size:
916 864 437
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1529 1562 2217
Size: 1529 33 655
Fragments arranged by size:
1529 655 33
BamHI G'GATC_C
Cuts at: 0 351 2217
Size: 351 1866
BanI G'GyrC_C
Cuts at: 0 1951 2217
Size: 1951 266
BanII G_rGCy'C
Cuts at: 0 1114 1199 1258 2217
Size: 1114 85 59 959
Fragments arranged by size:
1114 959 85 59
BbeI G_GCGC'C
Cuts at: 0 1955 2217
Size: 1955 262
BciVI GTATCCnnnnn_n'
Cuts at: 0 1356 2217
Size: 1356 861
BglII A'GATC_T
Cuts at: 0 332 947 2217
Size: 332 615 1270
Fragments arranged by size:
1270 615 332
Bme1580I G_kGCm'C
Cuts at: 0 286 623 1150 2217
Size: 286 337 527 1067
Fragments arranged by size:
1067 527 337 286
BmrI ACTGGGnnnn_n'
Cuts at: 0 1968 2217
Size: 1968 249
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 354 392 487 1218 2217
Size: 354 38 95 731 999
Fragments arranged by size:
999 731 354 95 38
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 400 463 514 743 792 1059 1327 1360
Size: 400 63 51 229 49 267 268 33
Cuts at: 1360 1591 2217
Size: 231 626
Fragments arranged by size:
626 400 268 267 231 229 63 51
49 33
Bpu10I CC'TnA_GC
Cuts at: 0 1108 2090 2217
Size: 1108 982 127
Fragments arranged by size:
1108 982 127
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 50 1623 2217
Size: 50 1573 594
Fragments arranged by size:
1573 594 50
BsaI GGTCTCn'nnnn_
Cuts at: 0 76 2217
Size: 76 2141
BsaHI Gr'CG_yC
Cuts at: 0 1952 2217
Size: 1952 265
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 531 561 974 1004 1838 1868 1916 1946
Size: 531 30 413 30 834 30 48 30
Cuts at: 1946 2217
Size: 271
Fragments arranged by size:
834 531 413 271 48 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1092 2217
Size: 1092 1125
BseYI C'CCAG_C
Cuts at: 0 385 2217
Size: 385 1832
BsiHKAI G_wGCw'C
Cuts at: 0 286 1150 1199 1258 2105 2217
Size: 286 864 49 59 847 112
Fragments arranged by size:
864 847 286 112 59 49
BsmI GAATG_Cn'
Cuts at: 0 1686 2217
Size: 1686 531
BsmBI CGTCTCn'nnnn_
Cuts at: 0 132 2217
Size: 132 2085
Bsp1286I G_dGCh'C
Cuts at: 0 286 623 1114 1150 1199 1258 2105 2217
Size: 286 337 491 36 49 59 847 112
Fragments arranged by size:
847 491 337 286 112 59 49 36
BspHI T'CATG_A
Cuts at: 0 473 2217
Size: 473 1744
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2139 2217
Size: 2139 78
BsrBI CCG'CTC
Cuts at: 0 1067 2217
Size: 1067 1150
BsrFI r'CCGG_y
Cuts at: 0 1301 2217
Size: 1301 916
BsrGI T'GTAC_A
Cuts at: 0 1867 2217
Size: 1867 350
BssSI C'ACGA_G
Cuts at: 0 1243 2098 2217
Size: 1243 855 119
Fragments arranged by size:
1243 855 119
BstBI TT'CG_AA
Cuts at: 0 1481 2217
Size: 1481 736
BstXI CCAn_nnnn'nTGG
Cuts at: 0 531 1892 2217
Size: 531 1361 325
Fragments arranged by size:
1361 531 325
BstYI r'GATC_y
Cuts at: 0 332 351 947 1215 2217
Size: 332 19 596 268 1002
Fragments arranged by size:
1002 596 332 268 19
BstZ17I GTA'TAC
Cuts at: 0 426 2217
Size: 426 1791
Bsu36I CC'TnA_GG
Cuts at: 0 347 2217
Size: 347 1870
DraI TTT'AAA
Cuts at: 0 75 2217
Size: 75 2142
EarI CTCTTCn'nnn_
Cuts at: 0 472 2055 2217
Size: 472 1583 162
Fragments arranged by size:
1583 472 162
EcoICRI GAG'CTC
Cuts at: 0 1197 1256 2217
Size: 1197 59 961
Fragments arranged by size:
1197 961 59
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 400 463 514 743 792 1059 1327 1360
Size: 400 63 51 229 49 267 268 33
Cuts at: 1360 1591 1924 2217
Size: 231 333 293
Fragments arranged by size:
400 333 293 268 267 231 229 63
51 49 33
EcoRI G'AATT_C
Cuts at: 0 1477 2217
Size: 1477 740
EcoRV GAT'ATC
Cuts at: 0 103 2217
Size: 103 2114
HaeII r_GCGC'y
Cuts at: 0 1955 2217
Size: 1955 262
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 112 144 177 209 231 263 496 528
Size: 112 32 33 32 22 32 233 32
Cuts at: 528 957 974 989 1006 1360 1392 1838 1870
Size: 429 17 15 17 354 32 446 32
Cuts at: 1870 2217
Size: 347
Fragments arranged by size:
446 429 354 347 233 112 33 32
32 32 32 32 32 22 17 17
15
HincII GTy'rAC
Cuts at: 0 998 2217
Size: 998 1219
HindIII A'AGCT_T
Cuts at: 0 68 2217
Size: 68 2149
KasI G'GCGC_C
Cuts at: 0 1951 2217
Size: 1951 266
MslI CAynn'nnrTG
Cuts at: 0 368 602 1466 2140 2217
Size: 368 234 864 674 77
Fragments arranged by size:
864 674 368 234 77
NarI GG'CG_CC
Cuts at: 0 1952 2217
Size: 1952 265
NspI r_CATG'y
Cuts at: 0 1004 1601 1874 2217
Size: 1004 597 273 343
Fragments arranged by size:
1004 597 343 273
PciI A'CATG_T
Cuts at: 0 1000 1870 2217
Size: 1000 870 347
Fragments arranged by size:
1000 870 347
PflMI CCAn_nnn'nTGG
Cuts at: 0 202 2217
Size: 202 2015
PfoI T'CCnGG_A
Cuts at: 0 1036 1568 2217
Size: 1036 532 649
Fragments arranged by size:
1036 649 532
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 256 288 1120 1152 2217
Size: 256 32 832 32 1065
Fragments arranged by size:
1065 832 256 32 32
SacI G_AGCT'C
Cuts at: 0 1199 1258 2217
Size: 1199 59 959
Fragments arranged by size:
1199 959 59
ScaI AGT'ACT
Cuts at: 0 1934 2217
Size: 1934 283
SfcI C'TryA_G
Cuts at: 0 799 1663 1943 2217
Size: 799 864 280 274
Fragments arranged by size:
864 799 280 274
SfoI GGC'GCC
Cuts at: 0 1953 2217
Size: 1953 264
SmlI C'TyrA_G
Cuts at: 0 65 1321 1602 2217
Size: 65 1256 281 615
Fragments arranged by size:
1256 615 281 65
StyI C'CwwG_G
Cuts at: 0 24 2217
Size: 24 2193
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 681 2217
Size: 681 1536
TatI w'GTAC_w
Cuts at: 0 1867 1932 2217
Size: 1867 65 285
Fragments arranged by size:
1867 285 65
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 311 343 851 883 1175 1207 2217
Size: 311 32 508 32 292 32 1010
Fragments arranged by size:
1010 508 311 292 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 124 988 1852 2217
Size: 124 864 864 365
Fragments arranged by size:
864 864 365 124
XbaI T'CTAG_A
Cuts at: 0 97 1380 2217
Size: 97 1283 837
Fragments arranged by size:
1283 837 97
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 170 767 1034 2217
Size: 170 597 267 1183
Fragments arranged by size:
1183 597 267 170
Enzymes that do cut:
AccI AcuI AflII AflIII AhdI AlwNI ApaLI ApoI
AseI BaeI BamHI BanI BanII BbeI BciVI BglII
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaHI
BsaXI BseRI BseYI BsiHKAI BsmI BsmBI Bsp1286I BspHI
BspMI BsrBI BsrFI BsrGI BssSI BstBI BstXI BstYI
BstZ17I Bsu36I DraI EarI EcoICRI Eco57MI EcoRI EcoRV
HaeII Hin4I HincII HindIII KasI MslI NarI NspI
PciI PflMI PfoI PpiI SacI ScaI SfcI SfoI
SmlI StyI TaqII TatI TstI Tth111I XbaI XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AgeI AleI AlfI
AloI ApaI AscI AsiSI AvaI AvrII BbsI BbvCI
BcgI BclI BfrBI BglI BlpI BmgBI BmtI BsaAI
BsaBI BsaWI BsgI BsiEI BsiWI BspEI BsrDI BssHII
BstAPI BstEII BtgI BtgZI BtsI ClaI CspCI DraIII
DrdI EaeI EagI EciI EcoNI EcoO109I FalI FseI
FspI FspAI HpaI KpnI MfeI MluI MmeI MscI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NruI
NsiI PacI PasI PmeI PmlI PpuMI PshAI PsiI
PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacII
SalI SanDI SapI SbfI SexAI SfiI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI
XhoI XmaI XmnI ZraI