Restriction map for
pFN21AE2074
(Linear) (Six-base) MAPSORT of: KIEE2074.seq Check: 8699 from: 1 to: 2211
KIEE2074.orf
With 182 enzymes: SgfI *
July 15, 2010 18:54 ..
AatII G_ACGT'C
Cuts at: 0 1022 2211
Size: 1022 1189
Acc65I G'GTAC_C
Cuts at: 0 1898 2211
Size: 1898 313
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 280 2211
Size: 280 1931
AfeI AGC'GCT
Cuts at: 0 382 2020 2211
Size: 382 1638 191
Fragments arranged by size:
1638 382 191
AflIII A'CryG_T
Cuts at: 0 519 2211
Size: 519 1692
AleI CACnn'nnGTG
Cuts at: 0 769 1967 2073 2211
Size: 769 1198 106 138
Fragments arranged by size:
1198 769 138 106
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 765 2211
Size: 765 1446
AlwNI CAG_nnn'CTG
Cuts at: 0 1721 2211
Size: 1721 490
ApaI G_GGCC'C
Cuts at: 0 375 2211
Size: 375 1836
ApaLI G'TGCA_C
Cuts at: 0 1329 1554 2066 2211
Size: 1329 225 512 145
Fragments arranged by size:
1329 512 225 145
ApoI r'AATT_y
Cuts at: 0 61 2211
Size: 61 2150
AvaI C'yCGr_G
Cuts at: 0 2163 2211
Size: 2163 48
AvrII C'CTAG_G
Cuts at: 0 1800 2211
Size: 1800 411
BamHI G'GATC_C
Cuts at: 0 1983 2211
Size: 1983 228
BanI G'GyrC_C
Cuts at: 0 358 646 1898 1923 2211
Size: 358 288 1252 25 288
Fragments arranged by size:
1252 358 288 288 25
BanII G_rGCy'C
Cuts at: 0 375 398 1874 2174 2211
Size: 375 23 1476 300 37
Fragments arranged by size:
1476 375 300 37 23
BbsI GAAGACnn'nnnn_
Cuts at: 0 753 1014 1274 1503 2015 2211
Size: 753 261 260 229 512 196
Fragments arranged by size:
753 512 261 260 229 196
BbvCI CC'TCA_GC
Cuts at: 0 363 1635 2176 2211
Size: 363 1272 541 35
Fragments arranged by size:
1272 541 363 35
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1867 1901 2075 2109 2211
Size: 1867 34 174 34 102
Fragments arranged by size:
1867 174 102 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 396 2211
Size: 396 1815
BfrBI ATG'CAT
Cuts at: 0 870 2211
Size: 870 1341
BglI GCCn_nnn'nGGC
Cuts at: 0 793 1388 2211
Size: 793 595 823
Fragments arranged by size:
823 793 595
BglII A'GATC_T
Cuts at: 0 710 2211
Size: 710 1501
Bme1580I G_kGCm'C
Cuts at: 0 375 579 1333 1558 2070 2211
Size: 375 204 754 225 512 141
Fragments arranged by size:
754 512 375 225 204 141
BmgBI CAC'GTC
Cuts at: 0 354 759 2211
Size: 354 405 1452
Fragments arranged by size:
1452 405 354
BmrI ACTGGGnnnn_n'
Cuts at: 0 60 1568 2211
Size: 60 1508 643
Fragments arranged by size:
1508 643 60
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 920 1461 1791 2211
Size: 920 541 330 420
Fragments arranged by size:
920 541 420 330
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 64 91 259 397 691 1048 1938 2023
Size: 64 27 168 138 294 357 890 85
Cuts at: 2023 2130 2211
Size: 107 81
Fragments arranged by size:
890 357 294 168 138 107 85 81
64 27
Bpu10I CC'TnA_GC
Cuts at: 0 363 1635 2176 2211
Size: 363 1272 541 35
Fragments arranged by size:
1272 541 363 35
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 793 2211
Size: 793 1418
BsaHI Gr'CG_yC
Cuts at: 0 1019 2211
Size: 1019 1192
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 523 1344 1437 1495 1993 2050 2091 2211
Size: 523 821 93 58 498 57 41 120
Fragments arranged by size:
821 523 498 120 93 58 57 41
BseYI C'CCAG_C
Cuts at: 0 624 2211
Size: 624 1587
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2079 2211
Size: 2079 132
BsiEI CG_ry'CG
Cuts at: 0 435 990 2211
Size: 435 555 1221
Fragments arranged by size:
1221 555 435
BsiHKAI G_wGCw'C
Cuts at: 0 86 347 398 1333 1558 1859 2070 2211
Size: 86 261 51 935 225 301 211 141
Fragments arranged by size:
935 301 261 225 211 141 86 51
Bsp1286I G_dGCh'C
Cuts at: 0 86 347 375 398 579 1333 1558 1859
Size: 86 261 28 23 181 754 225 301
Cuts at: 1859 1874 2070 2174 2211
Size: 15 196 104 37
Fragments arranged by size:
754 301 261 225 196 181 104 86
37 28 23 15
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 415 2211
Size: 415 1796
BsrFI r'CCGG_y
Cuts at: 0 643 985 1545 1661 1733 1805 2051 2211
Size: 643 342 560 116 72 72 246 160
Fragments arranged by size:
643 560 342 246 160 116 72 72
BssHII G'CGCG_C
Cuts at: 0 1822 2211
Size: 1822 389
BstAPI GCAn_nnn'nTGC
Cuts at: 0 293 2211
Size: 293 1918
BstBI TT'CG_AA
Cuts at: 0 89 2211
Size: 89 2122
BstXI CCAn_nnnn'nTGG
Cuts at: 0 624 937 2193 2211
Size: 624 313 1256 18
Fragments arranged by size:
1256 624 313 18
BstYI r'GATC_y
Cuts at: 0 710 1983 2211
Size: 710 1273 228
Fragments arranged by size:
1273 710 228
BtgI C'CryG_G
Cuts at: 0 424 650 1491 1909 2211
Size: 424 226 841 418 302
Fragments arranged by size:
841 424 418 302 226
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1608 2211
Size: 1608 603
BtsI GCAGTG_nn'
Cuts at: 0 31 86 747 1410 2211
Size: 31 55 661 663 801
Fragments arranged by size:
801 663 661 55 31
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 102 137 2211
Size: 102 35 2074
Fragments arranged by size:
2074 102 35
DraI TTT'AAA
Cuts at: 0 8 2211
Size: 8 2203
DraIII CAC_nnn'GTG
Cuts at: 0 505 2211
Size: 505 1706
EaeI y'GGCC_r
Cuts at: 0 987 1175 1912 2077 2138 2211
Size: 987 188 737 165 61 73
Fragments arranged by size:
987 737 188 165 73 61
EagI C'GGCC_G
Cuts at: 0 987 2211
Size: 987 1224
EarI CTCTTCn'nnn_
Cuts at: 0 91 256 1279 1399 2211
Size: 91 165 1023 120 812
Fragments arranged by size:
1023 812 165 120 91
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1369 1897 2211
Size: 1369 528 314
Fragments arranged by size:
1369 528 314
EcoICRI GAG'CTC
Cuts at: 0 396 2211
Size: 396 1815
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 64 91 259 280 397 691 1048 1938
Size: 64 27 168 21 117 294 357 890
Cuts at: 1938 2023 2130 2211
Size: 85 107 81
Fragments arranged by size:
890 357 294 168 117 107 85 81
64 27 21
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2197 2211
Size: 2197 14
EcoO109I rG'GnC_Cy
Cuts at: 0 221 243 331 372 2211
Size: 221 22 88 41 1839
Fragments arranged by size:
1839 221 88 41 22
EcoRI G'AATT_C
Cuts at: 0 61 2211
Size: 61 2150
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1287 2211
Size: 1287 924
FspI TGC'GCA
Cuts at: 0 1156 2211
Size: 1156 1055
HaeII r_GCGC'y
Cuts at: 0 315 384 633 1773 1837 2022 2211
Size: 315 69 249 1140 64 185 189
Fragments arranged by size:
1140 315 249 189 185 69 64
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1339 1371 1675 1707 2211
Size: 1339 32 304 32 504
Fragments arranged by size:
1339 504 304 32 32
HindIII A'AGCT_T
Cuts at: 0 1207 2211
Size: 1207 1004
KpnI G_GTAC'C
Cuts at: 0 1902 2211
Size: 1902 309
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 537 1028 1802 2211
Size: 537 491 774 409
Fragments arranged by size:
774 537 491 409
MslI CAynn'nnrTG
Cuts at: 0 418 536 769 935 1279 1561 1967 2073
Size: 418 118 233 166 344 282 406 106
Cuts at: 2073 2211
Size: 138
Fragments arranged by size:
418 406 344 282 233 166 138 118
106
MspA1I CmG'CkG
Cuts at: 0 1308 1755 2001 2111 2211
Size: 1308 447 246 110 100
Fragments arranged by size:
1308 447 246 110 100
NaeI GCC'GGC
Cuts at: 0 987 1735 2211
Size: 987 748 476
Fragments arranged by size:
987 748 476
NgoMIV G'CCGG_C
Cuts at: 0 985 1733 2211
Size: 985 748 478
Fragments arranged by size:
985 748 478
NsiI A_TGCA'T
Cuts at: 0 872 2211
Size: 872 1339
NspI r_CATG'y
Cuts at: 0 523 2211
Size: 523 1688
PasI CC'CwG_GG
Cuts at: 0 1893 2193 2211
Size: 1893 300 18
Fragments arranged by size:
1893 300 18
PciI A'CATG_T
Cuts at: 0 519 2211
Size: 519 1692
PfoI T'CCnGG_A
Cuts at: 0 347 911 1025 2211
Size: 347 564 114 1186
Fragments arranged by size:
1186 564 347 114
PpuMI rG'GwC_Cy
Cuts at: 0 221 2211
Size: 221 1990
PspOMI G'GGCC_C
Cuts at: 0 371 2211
Size: 371 1840
PstI C_TGCA'G
Cuts at: 0 143 2211
Size: 143 2068
SacI G_AGCT'C
Cuts at: 0 398 2211
Size: 398 1813
SapI GCTCTTCn'nnn_
Cuts at: 0 91 2211
Size: 91 2120
SexAI A'CCwGG_T
Cuts at: 0 475 2211
Size: 475 1736
SfcI C'TryA_G
Cuts at: 0 139 590 1713 2211
Size: 139 451 1123 498
Fragments arranged by size:
1123 498 451 139
SmlI C'TyrA_G
Cuts at: 0 808 2211
Size: 808 1403
StuI AGG'CCT
Cuts at: 0 1846 2211
Size: 1846 365
StyI C'CwwG_G
Cuts at: 0 1503 1531 1800 1841 2045 2211
Size: 1503 28 269 41 204 166
Fragments arranged by size:
1503 269 204 166 41 28
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 109 346 475 625 1348 2211
Size: 109 237 129 150 723 863
Fragments arranged by size:
863 723 237 150 129 109
Tth111I GACn'n_nGTC
Cuts at: 0 451 2211
Size: 451 1760
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 480 2190 2211
Size: 480 1710 21
Fragments arranged by size:
1710 480 21
ZraI GAC'GTC
Cuts at: 0 1020 2211
Size: 1020 1191
Enzymes that do cut:
AatII Acc65I AcuI AfeI AflIII AleI AlfI AlwNI
ApaI ApaLI ApoI AvaI AvrII BamHI BanI BanII
BbsI BbvCI BcgI BciVI BfrBI BglI BglII Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaHI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrFI BssHII
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI CspCI
DraI DraIII EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HindIII
KpnI MmeI MslI MspA1I NaeI NgoMIV NsiI NspI
PasI PciI PfoI PpuMI PspOMI PstI SacI SapI
SexAI SfcI SmlI StuI StyI TaqII Tth111I XcmI
ZraI
Enzymes that do not cut:
AarI AccI AclI AflII AgeI AhdI AloI AscI
AseI AsiSI BaeI BbeI BclI BlpI BmtI BsaI
BsaAI BsaBI BsaWI BsaXI BsiWI BsmI BsmBI BspEI
BspHI BsrBI BsrDI BsrGI BssSI BstEII BstZ17I Bsu36I
ClaI DrdI EcoRV FseI FspAI HincII HpaI KasI
MfeI MluI MscI NarI NcoI NdeI NheI NotI
NruI PacI PflMI PmeI PmlI PpiI PshAI PsiI
PspXI PsrI PvuI PvuII RsrII SacII SalI SanDI
SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI SwaI TatI TstI XbaI
XhoI XmaI XmnI