Restriction map for
pFN21AE2073
(Linear) (Six-base) MAPSORT of: KIEE2073.seq Check: 6184 from: 1 to: 2208
KIEE2073.orf
With 182 enzymes: SgfI *
July 15, 2010 18:54 ..
AccI GT'mk_AC
Cuts at: 0 718 2208
Size: 718 1490
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 697 1846 2208
Size: 697 1149 362
Fragments arranged by size:
1149 697 362
AflII C'TTAA_G
Cuts at: 0 2041 2208
Size: 2041 167
AflIII A'CryG_T
Cuts at: 0 1446 2208
Size: 1446 762
AlwNI CAG_nnn'CTG
Cuts at: 0 1639 2208
Size: 1639 569
ApaLI G'TGCA_C
Cuts at: 0 632 2208
Size: 632 1576
ApoI r'AATT_y
Cuts at: 0 101 286 1892 2202 2208
Size: 101 185 1606 310 6
Fragments arranged by size:
1606 310 185 101 6
AvaI C'yCGr_G
Cuts at: 0 74 2208
Size: 74 2134
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 237 270 2208
Size: 237 33 1938
Fragments arranged by size:
1938 237 33
BamHI G'GATC_C
Cuts at: 0 1491 2208
Size: 1491 717
BanI G'GyrC_C
Cuts at: 0 966 1779 2208
Size: 966 813 429
Fragments arranged by size:
966 813 429
BanII G_rGCy'C
Cuts at: 0 771 2208
Size: 771 1437
BbsI GAAGACnn'nnnn_
Cuts at: 0 845 910 1574 2208
Size: 845 65 664 634
Fragments arranged by size:
845 664 634 65
BbvCI CC'TCA_GC
Cuts at: 0 1408 1511 2208
Size: 1408 103 697
Fragments arranged by size:
1408 697 103
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1432 1466 2208
Size: 1432 34 742
Fragments arranged by size:
1432 742 34
BfrBI ATG'CAT
Cuts at: 0 28 982 1854 2208
Size: 28 954 872 354
Fragments arranged by size:
954 872 354 28
BglII A'GATC_T
Cuts at: 0 708 1168 2208
Size: 708 460 1040
Fragments arranged by size:
1040 708 460
BlpI GC'TnA_GC
Cuts at: 0 1646 2208
Size: 1646 562
Bme1580I G_kGCm'C
Cuts at: 0 636 969 2208
Size: 636 333 1239
Fragments arranged by size:
1239 636 333
BmrI ACTGGGnnnn_n'
Cuts at: 0 972 1218 2208
Size: 972 246 990
Fragments arranged by size:
990 972 246
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 755 1109 2208
Size: 755 354 1099
Fragments arranged by size:
1099 755 354
Bpu10I CC'TnA_GC
Cuts at: 0 1408 1511 2208
Size: 1408 103 697
Fragments arranged by size:
1408 697 103
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 568 1346 2208
Size: 568 778 862
Fragments arranged by size:
862 778 568
BsaI GGTCTCn'nnnn_
Cuts at: 0 2152 2208
Size: 2152 56
BsaAI yAC'GTr
Cuts at: 0 213 2208
Size: 213 1995
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1103 1133 1988 2018 2208
Size: 1103 30 855 30 190
Fragments arranged by size:
1103 855 190 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 533 1061 1106 1996 2208
Size: 533 528 45 890 212
Fragments arranged by size:
890 533 528 212 45
BseYI C'CCAG_C
Cuts at: 0 1886 1948 2208
Size: 1886 62 260
Fragments arranged by size:
1886 260 62
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1351 2208
Size: 1351 857
BsiHKAI G_wGCw'C
Cuts at: 0 636 771 2208
Size: 636 135 1437
Fragments arranged by size:
1437 636 135
BsmI GAATG_Cn'
Cuts at: 0 1355 2208
Size: 1355 853
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1360 2208
Size: 1360 848
Bsp1286I G_dGCh'C
Cuts at: 0 636 771 969 2208
Size: 636 135 198 1239
Fragments arranged by size:
1239 636 198 135
BspHI T'CATG_A
Cuts at: 0 1806 2208
Size: 1806 402
BsrBI CCG'CTC
Cuts at: 0 552 2208
Size: 552 1656
BssSI C'ACGA_G
Cuts at: 0 2149 2208
Size: 2149 59
BstAPI GCAn_nnn'nTGC
Cuts at: 0 34 2208
Size: 34 2174
BstBI TT'CG_AA
Cuts at: 0 1266 2208
Size: 1266 942
BstYI r'GATC_y
Cuts at: 0 708 1168 1491 2093 2208
Size: 708 460 323 602 115
Fragments arranged by size:
708 602 460 323 115
BstZ17I GTA'TAC
Cuts at: 0 719 2208
Size: 719 1489
BtsI GCAGTG_nn'
Cuts at: 0 834 2208
Size: 834 1374
DraI TTT'AAA
Cuts at: 0 1052 2208
Size: 1052 1156
EaeI y'GGCC_r
Cuts at: 0 1459 2208
Size: 1459 749
EarI CTCTTCn'nnn_
Cuts at: 0 1079 1521 2208
Size: 1079 442 687
Fragments arranged by size:
1079 687 442
EcoICRI GAG'CTC
Cuts at: 0 769 2208
Size: 769 1439
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 697 755 1109 1846 2208
Size: 697 58 354 737 362
Fragments arranged by size:
737 697 362 354 58
HaeII r_GCGC'y
Cuts at: 0 887 2208
Size: 887 1321
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 923 955 1988 2020 2208
Size: 923 32 1033 32 188
Fragments arranged by size:
1033 923 188 32 32
HincII GTy'rAC
Cuts at: 0 942 2208
Size: 942 1266
MfeI C'AATT_G
Cuts at: 0 2065 2208
Size: 2065 143
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 558 2208
Size: 558 1650
MscI TGG'CCA
Cuts at: 0 1461 2208
Size: 1461 747
MslI CAynn'nnrTG
Cuts at: 0 1451 1969 2208
Size: 1451 518 239
Fragments arranged by size:
1451 518 239
NdeI CA'TA_TG
Cuts at: 0 30 96 582 620 1254 2208
Size: 30 66 486 38 634 954
Fragments arranged by size:
954 634 486 66 38 30
NsiI A_TGCA'T
Cuts at: 0 30 984 1856 2208
Size: 30 954 872 352
Fragments arranged by size:
954 872 352 30
NspI r_CATG'y
Cuts at: 0 986 1102 1450 1872 2208
Size: 986 116 348 422 336
Fragments arranged by size:
986 422 348 336 116
PciI A'CATG_T
Cuts at: 0 1446 2208
Size: 1446 762
PflMI CCAn_nnn'nTGG
Cuts at: 0 757 2103 2208
Size: 757 1346 105
Fragments arranged by size:
1346 757 105
SacI G_AGCT'C
Cuts at: 0 771 2208
Size: 771 1437
ScaI AGT'ACT
Cuts at: 0 438 2208
Size: 438 1770
SfcI C'TryA_G
Cuts at: 0 423 627 2208
Size: 423 204 1581
Fragments arranged by size:
1581 423 204
SmlI C'TyrA_G
Cuts at: 0 547 1361 2041 2208
Size: 547 814 680 167
Fragments arranged by size:
814 680 547 167
SnaBI TAC'GTA
Cuts at: 0 213 2208
Size: 213 1995
SphI G_CATG'C
Cuts at: 0 986 2208
Size: 986 1222
SspI AAT'ATT
Cuts at: 0 1182 1378 2208
Size: 1182 196 830
Fragments arranged by size:
1182 830 196
StyI C'CwwG_G
Cuts at: 0 476 1544 2208
Size: 476 1068 664
Fragments arranged by size:
1068 664 476
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 984 1145 2208
Size: 984 161 1063
Fragments arranged by size:
1063 984 161
TatI w'GTAC_w
Cuts at: 0 273 436 1110 1825 2127 2208
Size: 273 163 674 715 302 81
Fragments arranged by size:
715 674 302 273 163 81
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 838 870 2208
Size: 838 32 1338
Fragments arranged by size:
1338 838 32
XmnI GAAnn'nnTTC
Cuts at: 0 1262 2208
Size: 1262 946
Enzymes that do cut:
AccI AcuI AflII AflIII AlwNI ApaLI ApoI AvaI
BaeI BamHI BanI BanII BbsI BbvCI BcgI BfrBI
BglII BlpI Bme1580I BmrI BpmI Bpu10I BpuEI BsaI
BsaAI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI
Bsp1286I BspHI BsrBI BssSI BstAPI BstBI BstYI BstZ17I
BtsI DraI EaeI EarI EcoICRI Eco57MI HaeII Hin4I
HincII MfeI MmeI MscI MslI NdeI NsiI NspI
PciI PflMI SacI ScaI SfcI SmlI SnaBI SphI
SspI StyI TaqII TatI TstI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AgeI AhdI AleI
AlfI AloI ApaI AscI AseI AsiSI AvrII BbeI
BciVI BclI BglI BmgBI BmtI BplI BsaBI BsaHI
BsaWI BsiEI BsiWI BspEI BspMI BsrDI BsrFI BsrGI
BssHII BstEII BstXI Bsu36I BtgI BtgZI ClaI CspCI
DraIII DrdI EagI EciI EcoNI EcoO109I EcoRI EcoRV
FalI FseI FspI FspAI HindIII HpaI KasI KpnI
MluI MspA1I NaeI NarI NcoI NgoMIV NheI NotI
NruI PacI PasI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII
RsrII SacII SalI SanDI SapI SbfI SexAI SfiI
SfoI SgfI SgrAI SmaI SpeI SrfI StuI SwaI
Tth111I XbaI XcmI XhoI XmaI ZraI