Restriction map for
pFN21AE2072
(Linear) (Six-base) MAPSORT of: KIEE2072.seq Check: 8939 from: 1 to: 2208
KIEE2072.orf
With 182 enzymes: SgfI *
July 15, 2010 18:54 ..
AccI GT'mk_AC
Cuts at: 0 1249 2208
Size: 1249 959
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1606 2087 2208
Size: 1606 481 121
Fragments arranged by size:
1606 481 121
AgeI A'CCGG_T
Cuts at: 0 716 2208
Size: 716 1492
AlwNI CAG_nnn'CTG
Cuts at: 0 1828 1949 2009 2019 2208
Size: 1828 121 60 10 189
Fragments arranged by size:
1828 189 121 60 10
ApoI r'AATT_y
Cuts at: 0 1383 1753 2208
Size: 1383 370 455
Fragments arranged by size:
1383 455 370
AseI AT'TA_AT
Cuts at: 0 940 2208
Size: 940 1268
BamHI G'GATC_C
Cuts at: 0 324 2208
Size: 324 1884
BbsI GAAGACnn'nnnn_
Cuts at: 0 1034 1193 1281 1594 2192 2208
Size: 1034 159 88 313 598 16
Fragments arranged by size:
1034 598 313 159 88 16
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 210 244 2208
Size: 210 34 1964
Fragments arranged by size:
1964 210 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 2196 2208
Size: 2196 12
BclI T'GATC_A
Cuts at: 0 1344 2208
Size: 1344 864
BfrBI ATG'CAT
Cuts at: 0 945 1097 2208
Size: 945 152 1111
Fragments arranged by size:
1111 945 152
Bme1580I G_kGCm'C
Cuts at: 0 380 2208
Size: 380 1828
BmgBI CAC'GTC
Cuts at: 0 1206 2208
Size: 1206 1002
BmrI ACTGGGnnnn_n'
Cuts at: 0 1239 2208
Size: 1239 969
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 138 600 688 1373 2208
Size: 138 462 88 685 835
Fragments arranged by size:
835 685 462 138 88
Bpu10I CC'TnA_GC
Cuts at: 0 138 1693 1913 2208
Size: 138 1555 220 295
Fragments arranged by size:
1555 295 220 138
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 90 2208
Size: 90 2118
BsaI GGTCTCn'nnnn_
Cuts at: 0 694 1118 2208
Size: 694 424 1090
Fragments arranged by size:
1090 694 424
BsaWI w'CCGG_w
Cuts at: 0 716 2208
Size: 716 1492
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 117 147 2208
Size: 117 30 2061
Fragments arranged by size:
2061 117 30
BseYI C'CCAG_C
Cuts at: 0 346 591 2208
Size: 346 245 1617
Fragments arranged by size:
1617 346 245
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1210 1706 2208
Size: 1210 496 502
Fragments arranged by size:
1210 502 496
BsiEI CG_ry'CG
Cuts at: 0 1653 2208
Size: 1653 555
BsmI GAATG_Cn'
Cuts at: 0 761 1655 2208
Size: 761 894 553
Fragments arranged by size:
894 761 553
Bsp1286I G_dGCh'C
Cuts at: 0 380 2208
Size: 380 1828
BspHI T'CATG_A
Cuts at: 0 911 1484 2208
Size: 911 573 724
Fragments arranged by size:
911 724 573
BsrBI CCG'CTC
Cuts at: 0 1779 2208
Size: 1779 429
BsrDI GCAATG_nn'
Cuts at: 0 251 662 1735 2174 2208
Size: 251 411 1073 439 34
Fragments arranged by size:
1073 439 411 251 34
BsrFI r'CCGG_y
Cuts at: 0 716 2208
Size: 716 1492
BstXI CCAn_nnnn'nTGG
Cuts at: 0 925 1023 1713 2208
Size: 925 98 690 495
Fragments arranged by size:
925 690 495 98
BstYI r'GATC_y
Cuts at: 0 324 860 1304 2208
Size: 324 536 444 904
Fragments arranged by size:
904 536 444 324
Bsu36I CC'TnA_GG
Cuts at: 0 197 2208
Size: 197 2011
BtgI C'CryG_G
Cuts at: 0 2198 2208
Size: 2198 10
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 457 497 2208
Size: 457 40 1711
Fragments arranged by size:
1711 457 40
BtsI GCAGTG_nn'
Cuts at: 0 226 2208
Size: 226 1982
DrdI GACnn_nn'nnGTC
Cuts at: 0 717 1864 2208
Size: 717 1147 344
Fragments arranged by size:
1147 717 344
EaeI y'GGCC_r
Cuts at: 0 1013 2208
Size: 1013 1195
EarI CTCTTCn'nnn_
Cuts at: 0 345 462 467 2208
Size: 345 117 5 1741
Fragments arranged by size:
1741 345 117 5
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 138 600 688 1373 1606 2087 2208
Size: 138 462 88 685 233 481 121
Fragments arranged by size:
685 481 462 233 138 121 88
EcoNI CCTnn'n_nnAGG
Cuts at: 0 305 1015 2208
Size: 305 710 1193
Fragments arranged by size:
1193 710 305
EcoO109I rG'GnC_Cy
Cuts at: 0 513 587 1662 2071 2208
Size: 513 74 1075 409 137
Fragments arranged by size:
1075 513 409 137 74
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 483 515 850 852 882 884 2208
Size: 483 32 335 2 30 2 1324
Fragments arranged by size:
1324 483 335 32 30 2 2
HincII GTy'rAC
Cuts at: 0 624 990 1250 2208
Size: 624 366 260 958
Fragments arranged by size:
958 624 366 260
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 292 1403 2208
Size: 292 1111 805
Fragments arranged by size:
1111 805 292
MscI TGG'CCA
Cuts at: 0 1015 2208
Size: 1015 1193
MslI CAynn'nnrTG
Cuts at: 0 482 1483 2208
Size: 482 1001 725
Fragments arranged by size:
1001 725 482
MspA1I CmG'CkG
Cuts at: 0 346 595 1327 2208
Size: 346 249 732 881
Fragments arranged by size:
881 732 346 249
NdeI CA'TA_TG
Cuts at: 0 1565 2208
Size: 1565 643
NsiI A_TGCA'T
Cuts at: 0 947 1099 2208
Size: 947 152 1109
Fragments arranged by size:
1109 947 152
NspI r_CATG'y
Cuts at: 0 1097 2208
Size: 1097 1111
PasI CC'CwG_GG
Cuts at: 0 2154 2208
Size: 2154 54
PfoI T'CCnGG_A
Cuts at: 0 1115 1350 2208
Size: 1115 235 858
Fragments arranged by size:
1115 858 235
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2033 2065 2208
Size: 2033 32 143
Fragments arranged by size:
2033 143 32
PpuMI rG'GwC_Cy
Cuts at: 0 513 587 1662 2071 2208
Size: 513 74 1075 409 137
Fragments arranged by size:
1075 513 409 137 74
PsiI TTA'TAA
Cuts at: 0 1165 2208
Size: 1165 1043
PvuI CG_AT'CG
Cuts at: 0 1653 2208
Size: 1653 555
PvuII CAG'CTG
Cuts at: 0 595 1327 2208
Size: 595 732 881
Fragments arranged by size:
881 732 595
SalI G'TCGA_C
Cuts at: 0 1248 2208
Size: 1248 960
SapI GCTCTTCn'nnn_
Cuts at: 0 345 2208
Size: 345 1863
SexAI A'CCwGG_T
Cuts at: 0 806 2208
Size: 806 1402
SfcI C'TryA_G
Cuts at: 0 81 405 2208
Size: 81 324 1803
Fragments arranged by size:
1803 324 81
SmlI C'TyrA_G
Cuts at: 0 69 2208
Size: 69 2139
StyI C'CwwG_G
Cuts at: 0 1016 1712 2208
Size: 1016 696 496
Fragments arranged by size:
1016 696 496
Tth111I GACn'n_nGTC
Cuts at: 0 1285 2208
Size: 1285 923
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1710 2208
Size: 1710 498
XmnI GAAnn'nnTTC
Cuts at: 0 456 2208
Size: 456 1752
Enzymes that do cut:
AccI AcuI AgeI AlwNI ApoI AseI BamHI BbsI
BcgI BciVI BclI BfrBI Bme1580I BmgBI BmrI BpmI
Bpu10I BpuEI BsaI BsaWI BsaXI BseYI BsgI BsiEI
BsmI Bsp1286I BspHI BsrBI BsrDI BsrFI BstXI BstYI
Bsu36I BtgI BtgZI BtsI DrdI EaeI EarI Eco57MI
EcoNI EcoO109I Hin4I HincII MmeI MscI MslI MspA1I
NdeI NsiI NspI PasI PfoI PpiI PpuMI PsiI
PvuI PvuII SalI SapI SexAI SfcI SmlI StyI
Tth111I XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AhdI
AleI AlfI AloI ApaI ApaLI AscI AsiSI AvaI
AvrII BaeI BanI BanII BbeI BbvCI BglI BglII
BlpI BmtI BplI BsaAI BsaBI BsaHI BseRI BsiHKAI
BsiWI BsmBI BspEI BspMI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstZ17I ClaI CspCI DraI DraIII EagI
EciI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI
HaeII HindIII HpaI KasI KpnI MfeI MluI NaeI
NarI NcoI NgoMIV NheI NotI NruI PacI PciI
PflMI PmeI PmlI PshAI PspOMI PspXI PsrI PstI
RsrII SacI SacII SanDI SbfI ScaI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
StuI SwaI TaqII TatI TstI XbaI XhoI XmaI
ZraI