Restriction map for
pFN21AE2071
(Linear) (Six-base) MAPSORT of: KIEE2071.seq Check: 8122 from: 1 to: 2190
KIEE2071.orf
With 182 enzymes: SgfI *
July 15, 2010 18:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 340 1560 2190
Size: 340 1220 630
Fragments arranged by size:
1220 630 340
Acc65I G'GTAC_C
Cuts at: 0 1843 2190
Size: 1843 347
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1871 2190
Size: 1871 319
AflIII A'CryG_T
Cuts at: 0 192 887 2190
Size: 192 695 1303
Fragments arranged by size:
1303 695 192
AleI CACnn'nnGTG
Cuts at: 0 1093 2190
Size: 1093 1097
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1644 2190
Size: 1644 546
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 131 163 2190
Size: 131 32 2027
Fragments arranged by size:
2027 131 32
AlwNI CAG_nnn'CTG
Cuts at: 0 703 2190
Size: 703 1487
ApoI r'AATT_y
Cuts at: 0 367 612 2190
Size: 367 245 1578
Fragments arranged by size:
1578 367 245
BanI G'GyrC_C
Cuts at: 0 1843 2190
Size: 1843 347
BanII G_rGCy'C
Cuts at: 0 564 2190
Size: 564 1626
BbsI GAAGACnn'nnnn_
Cuts at: 0 50 916 1793 2190
Size: 50 866 877 397
Fragments arranged by size:
877 866 397 50
BciVI GTATCCnnnnn_n'
Cuts at: 0 498 2190
Size: 498 1692
BclI T'GATC_A
Cuts at: 0 354 2190
Size: 354 1836
Bme1580I G_kGCm'C
Cuts at: 0 1353 2190
Size: 1353 837
BmgBI CAC'GTC
Cuts at: 0 1091 2190
Size: 1091 1099
BmrI ACTGGGnnnn_n'
Cuts at: 0 139 911 1518 2190
Size: 139 772 607 672
Fragments arranged by size:
772 672 607 139
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1647 2190
Size: 1647 543
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 902 1385 1699 2190
Size: 902 483 314 491
Fragments arranged by size:
902 491 483 314
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 412 990 1064 1653 2190
Size: 412 578 74 589 537
Fragments arranged by size:
589 578 537 412 74
BsaI GGTCTCn'nnnn_
Cuts at: 0 1041 1361 1436 1555 1914 2190
Size: 1041 320 75 119 359 276
Fragments arranged by size:
1041 359 320 276 119 75
BsaAI yAC'GTr
Cuts at: 0 736 2190
Size: 736 1454
BsaHI Gr'CG_yC
Cuts at: 0 38 2190
Size: 38 2152
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 131 161 2190
Size: 131 30 2029
Fragments arranged by size:
2029 131 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1198 1449 1671 2190
Size: 1198 251 222 519
Fragments arranged by size:
1198 519 251 222
BseYI C'CCAG_C
Cuts at: 0 29 2107 2190
Size: 29 2078 83
Fragments arranged by size:
2078 83 29
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 120 2112 2190
Size: 120 1992 78
Fragments arranged by size:
1992 120 78
BsiHKAI G_wGCw'C
Cuts at: 0 20 564 1067 2190
Size: 20 544 503 1123
Fragments arranged by size:
1123 544 503 20
BsmI GAATG_Cn'
Cuts at: 0 300 2190
Size: 300 1890
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1470 1871 2190
Size: 1470 401 319
Fragments arranged by size:
1470 401 319
Bsp1286I G_dGCh'C
Cuts at: 0 20 564 1067 1353 2190
Size: 20 544 503 286 837
Fragments arranged by size:
837 544 503 286 20
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 340 1560 2190
Size: 340 1220 630
Fragments arranged by size:
1220 630 340
BsrBI CCG'CTC
Cuts at: 0 2072 2190
Size: 2072 118
BsrDI GCAATG_nn'
Cuts at: 0 2080 2190
Size: 2080 110
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1348 2190
Size: 1348 842
BstEII G'GTnAC_C
Cuts at: 0 1512 1777 2094 2190
Size: 1512 265 317 96
Fragments arranged by size:
1512 317 265 96
BstYI r'GATC_y
Cuts at: 0 1758 2190
Size: 1758 432
BtgI C'CryG_G
Cuts at: 0 1211 1994 2190
Size: 1211 783 196
Fragments arranged by size:
1211 783 196
BtsI GCAGTG_nn'
Cuts at: 0 108 2190
Size: 108 2082
EaeI y'GGCC_r
Cuts at: 0 1734 1991 2190
Size: 1734 257 199
Fragments arranged by size:
1734 257 199
EarI CTCTTCn'nnn_
Cuts at: 0 10 77 177 474 1382 1440 1699 1916
Size: 10 67 100 297 908 58 259 217
Cuts at: 1916 2190
Size: 274
Fragments arranged by size:
908 297 274 259 217 100 67 58
10
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 10 2190
Size: 10 2180
EcoICRI GAG'CTC
Cuts at: 0 562 2190
Size: 562 1628
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 902 1385 1699 1871 2190
Size: 902 483 314 172 319
Fragments arranged by size:
902 483 319 314 172
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1795 2190
Size: 1795 395
EcoO109I rG'GnC_Cy
Cuts at: 0 1810 2190
Size: 1810 380
EcoRI G'AATT_C
Cuts at: 0 612 2190
Size: 612 1578
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 285 2190
Size: 285 1905
HaeII r_GCGC'y
Cuts at: 0 1167 2190
Size: 1167 1023
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 78 110 1075 1107 1439 1471 1560 1592
Size: 78 32 965 32 332 32 89 32
Cuts at: 1592 1849 1881 2008 2040 2190
Size: 257 32 127 32 150
Fragments arranged by size:
965 332 257 150 127 89 78 32
32 32 32 32 32
KpnI G_GTAC'C
Cuts at: 0 1847 2190
Size: 1847 343
MscI TGG'CCA
Cuts at: 0 1736 1993 2190
Size: 1736 257 197
Fragments arranged by size:
1736 257 197
MslI CAynn'nnrTG
Cuts at: 0 293 886 1093 2190
Size: 293 593 207 1097
Fragments arranged by size:
1097 593 293 207
NcoI C'CATG_G
Cuts at: 0 1994 2190
Size: 1994 196
NdeI CA'TA_TG
Cuts at: 0 1027 2190
Size: 1027 1163
NspI r_CATG'y
Cuts at: 0 196 407 891 2190
Size: 196 211 484 1299
Fragments arranged by size:
1299 484 211 196
PasI CC'CwG_GG
Cuts at: 0 1301 2190
Size: 1301 889
PciI A'CATG_T
Cuts at: 0 192 887 2190
Size: 192 695 1303
Fragments arranged by size:
1303 695 192
PflMI CCAn_nnn'nTGG
Cuts at: 0 371 1046 2190
Size: 371 675 1144
Fragments arranged by size:
1144 675 371
PfoI T'CCnGG_A
Cuts at: 0 2059 2190
Size: 2059 131
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 131 163 1893 1925 2190
Size: 131 32 1730 32 265
Fragments arranged by size:
1730 265 131 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 1810 2190
Size: 1810 380
SacI G_AGCT'C
Cuts at: 0 564 2190
Size: 564 1626
SapI GCTCTTCn'nnn_
Cuts at: 0 10 2190
Size: 10 2180
ScaI AGT'ACT
Cuts at: 0 1284 2190
Size: 1284 906
SexAI A'CCwGG_T
Cuts at: 0 508 2190
Size: 508 1682
SmlI C'TyrA_G
Cuts at: 0 427 1005 1079 1668 2190
Size: 427 578 74 589 522
Fragments arranged by size:
589 578 522 427 74
SnaBI TAC'GTA
Cuts at: 0 736 2190
Size: 736 1454
StyI C'CwwG_G
Cuts at: 0 1421 1994 2190
Size: 1421 573 196
Fragments arranged by size:
1421 573 196
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1500 1554 2067 2190
Size: 1500 54 513 123
Fragments arranged by size:
1500 513 123 54
TatI w'GTAC_w
Cuts at: 0 195 1282 2190
Size: 195 1087 908
Fragments arranged by size:
1087 908 195
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 496 528 2190
Size: 496 32 1662
Fragments arranged by size:
1662 496 32
XbaI T'CTAG_A
Cuts at: 0 1639 2190
Size: 1639 551
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 31 2190
Size: 31 2159
XmnI GAAnn'nnTTC
Cuts at: 0 268 1524 1748 2190
Size: 268 1256 224 442
Fragments arranged by size:
1256 442 268 224
Enzymes that do cut:
AarI Acc65I AcuI AflIII AleI AlfI AloI AlwNI
ApoI BanI BanII BbsI BciVI BclI Bme1580I BmgBI
BmrI BplI BpmI BpuEI BsaI BsaAI BsaHI BsaXI
BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspMI
BsrBI BsrDI BstAPI BstEII BstYI BtgI BtsI EaeI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
HaeII Hin4I KpnI MscI MslI NcoI NdeI NspI
PasI PciI PflMI PfoI PpiI PpuMI SacI SapI
ScaI SexAI SmlI SnaBI StyI TaqII TatI TstI
XbaI XcmI XmnI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AgeI AhdI ApaI
ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI
BbeI BbvCI BcgI BfrBI BglI BglII BlpI BmtI
Bpu10I BsaBI BsaWI BsiEI BsiWI BspEI BspHI BsrFI
BsrGI BssHII BssSI BstBI BstXI BstZ17I Bsu36I BtgZI
ClaI CspCI DraI DraIII DrdI EagI EcoRV FseI
FspI FspAI HincII HindIII HpaI KasI MfeI MluI
MmeI MspA1I NaeI NarI NgoMIV NheI NotI NruI
NsiI PacI PmeI PmlI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI PvuII RsrII SacII SalI SanDI
SbfI SfcI SfiI SfoI SgfI SgrAI SmaI SpeI
SphI SrfI SspI StuI SwaI Tth111I XhoI XmaI
ZraI