Restriction map for
pFN21AE2064
(Linear) (Six-base) MAPSORT of: KIEE2064.seq Check: 2494 from: 1 to: 2136
KIEE2064.orf
With 182 enzymes: SgfI *
July 15, 2010 18:54 ..
Acc65I G'GTAC_C
Cuts at: 0 1898 2136
Size: 1898 238
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1099 2108 2136
Size: 1099 1009 28
Fragments arranged by size:
1099 1009 28
AfeI AGC'GCT
Cuts at: 0 133 2136
Size: 133 2003
AflIII A'CryG_T
Cuts at: 0 1499 2136
Size: 1499 637
AhdI GACnn_n'nnGTC
Cuts at: 0 291 2136
Size: 291 1845
AleI CACnn'nnGTG
Cuts at: 0 1201 2136
Size: 1201 935
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 51 1434 2136
Size: 51 1383 702
Fragments arranged by size:
1383 702 51
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 757 789 2136
Size: 757 32 1347
Fragments arranged by size:
1347 757 32
AlwNI CAG_nnn'CTG
Cuts at: 0 474 612 916 1317 1455 1620 1866 2104
Size: 474 138 304 401 138 165 246 238
Cuts at: 2104 2136
Size: 32
Fragments arranged by size:
474 401 304 246 238 165 138 138
32
ApaI G_GGCC'C
Cuts at: 0 282 2136
Size: 282 1854
ApaLI G'TGCA_C
Cuts at: 0 1624 2136
Size: 1624 512
AvaI C'yCGr_G
Cuts at: 0 1225 1942 2136
Size: 1225 717 194
Fragments arranged by size:
1225 717 194
AvrII C'CTAG_G
Cuts at: 0 531 2136
Size: 531 1605
BamHI G'GATC_C
Cuts at: 0 144 716 2136
Size: 144 572 1420
Fragments arranged by size:
1420 572 144
BanI G'GyrC_C
Cuts at: 0 110 644 1898 2136
Size: 110 534 1254 238
Fragments arranged by size:
1254 534 238 110
BanII G_rGCy'C
Cuts at: 0 84 187 200 263 282 887 2136
Size: 84 103 13 63 19 605 1249
Fragments arranged by size:
1249 605 103 84 63 19 13
BbeI G_GCGC'C
Cuts at: 0 114 2136
Size: 114 2022
BbsI GAAGACnn'nnnn_
Cuts at: 0 980 1087 2136
Size: 980 107 1049
Fragments arranged by size:
1049 980 107
BbvCI CC'TCA_GC
Cuts at: 0 839 909 1438 2136
Size: 839 70 529 698
Fragments arranged by size:
839 698 529 70
BglI GCCn_nnn'nGGC
Cuts at: 0 1153 1186 1228 2050 2136
Size: 1153 33 42 822 86
Fragments arranged by size:
1153 822 86 42 33
Bme1580I G_kGCm'C
Cuts at: 0 282 1628 2136
Size: 282 1346 508
Fragments arranged by size:
1346 508 282
BmgBI CAC'GTC
Cuts at: 0 1185 1287 2136
Size: 1185 102 849
Fragments arranged by size:
1185 849 102
BmrI ACTGGGnnnn_n'
Cuts at: 0 1185 2136
Size: 1185 951
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 71 714 801 2136
Size: 71 643 87 1335
Fragments arranged by size:
1335 643 87 71
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 311 1390 1900 2005 2136
Size: 311 1079 510 105 131
Fragments arranged by size:
1079 510 311 131 105
Bpu10I CC'TnA_GC
Cuts at: 0 181 199 839 909 1438 2136
Size: 181 18 640 70 529 698
Fragments arranged by size:
698 640 529 181 70 18
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1516 2136
Size: 1516 620
BsaI GGTCTCn'nnnn_
Cuts at: 0 1308 1540 1709 2136
Size: 1308 232 169 427
Fragments arranged by size:
1308 427 232 169
BsaHI Gr'CG_yC
Cuts at: 0 111 457 1358 2136
Size: 111 346 901 778
Fragments arranged by size:
901 778 346 111
BsaWI w'CCGG_w
Cuts at: 0 147 2136
Size: 147 1989
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 288 405 856 897 964 1567 2002 2023
Size: 288 117 451 41 67 603 435 21
Cuts at: 2023 2035 2136
Size: 12 101
Fragments arranged by size:
603 451 435 288 117 101 67 41
21 12
BseYI C'CCAG_C
Cuts at: 0 1095 2136
Size: 1095 1041
BsiEI CG_ry'CG
Cuts at: 0 63 193 1599 2136
Size: 63 130 1406 537
Fragments arranged by size:
1406 537 130 63
BsiHKAI G_wGCw'C
Cuts at: 0 200 1628 2136
Size: 200 1428 508
Fragments arranged by size:
1428 508 200
BsmBI CGTCTCn'nnnn_
Cuts at: 0 616 655 2136
Size: 616 39 1481
Fragments arranged by size:
1481 616 39
Bsp1286I G_dGCh'C
Cuts at: 0 84 187 200 263 282 887 1628 2136
Size: 84 103 13 63 19 605 741 508
Fragments arranged by size:
741 605 508 103 84 63 19 13
BspEI T'CCGG_A
Cuts at: 0 147 2136
Size: 147 1989
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 32 1082 1127 1874 2136
Size: 32 1050 45 747 262
Fragments arranged by size:
1050 747 262 45 32
BsrBI CCG'CTC
Cuts at: 0 368 758 1068 1947 2136
Size: 368 390 310 879 189
Fragments arranged by size:
879 390 368 310 189
BsrDI GCAATG_nn'
Cuts at: 0 532 1808 2136
Size: 532 1276 328
Fragments arranged by size:
1276 532 328
BsrFI r'CCGG_y
Cuts at: 0 1028 2092 2136
Size: 1028 1064 44
Fragments arranged by size:
1064 1028 44
BsrGI T'GTAC_A
Cuts at: 0 1349 2136
Size: 1349 787
BssSI C'ACGA_G
Cuts at: 0 264 2136
Size: 264 1872
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1206 2136
Size: 1206 930
BstEII G'GTnAC_C
Cuts at: 0 831 2136
Size: 831 1305
BstYI r'GATC_y
Cuts at: 0 144 716 1091 1910 2136
Size: 144 572 375 819 226
Fragments arranged by size:
819 572 375 226 144
BtgI C'CryG_G
Cuts at: 0 158 187 1538 2136
Size: 158 29 1351 598
Fragments arranged by size:
1351 598 158 29
DraIII CAC_nnn'GTG
Cuts at: 0 684 1287 2136
Size: 684 603 849
Fragments arranged by size:
849 684 603
DrdI GACnn_nn'nnGTC
Cuts at: 0 741 2136
Size: 741 1395
EaeI y'GGCC_r
Cuts at: 0 60 190 422 476 959 1109 1490 1523
Size: 60 130 232 54 483 150 381 33
Cuts at: 1523 1694 2136
Size: 171 442
Fragments arranged by size:
483 442 381 232 171 150 130 60
54 33
EagI C'GGCC_G
Cuts at: 0 60 190 2136
Size: 60 130 1946
Fragments arranged by size:
1946 130 60
EarI CTCTTCn'nnn_
Cuts at: 0 1994 2012 2024 2136
Size: 1994 18 12 112
Fragments arranged by size:
1994 112 18 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1027 2136
Size: 1027 1109
EcoICRI GAG'CTC
Cuts at: 0 198 2136
Size: 198 1938
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 311 1099 1390 1900 2005 2108 2136
Size: 311 788 291 510 105 103 28
Fragments arranged by size:
788 510 311 291 105 103 28
EcoNI CCTnn'n_nnAGG
Cuts at: 0 234 2136
Size: 234 1902
EcoO109I rG'GnC_Cy
Cuts at: 0 177 278 279 471 550 846 1071 1863
Size: 177 101 1 192 79 296 225 792
Cuts at: 1863 2136
Size: 273
Fragments arranged by size:
792 296 273 225 192 177 101 79
1
EcoRV GAT'ATC
Cuts at: 0 1425 2136
Size: 1425 711
FspI TGC'GCA
Cuts at: 0 1465 2136
Size: 1465 671
HaeII r_GCGC'y
Cuts at: 0 114 135 243 1277 1345 1445 2136
Size: 114 21 108 1034 68 100 691
Fragments arranged by size:
1034 691 114 108 100 68 21
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 277 309 1714 1746 2136
Size: 277 32 1405 32 390
Fragments arranged by size:
1405 390 277 32 32
HincII GTy'rAC
Cuts at: 0 734 2136
Size: 734 1402
KasI G'GCGC_C
Cuts at: 0 110 2136
Size: 110 2026
KpnI G_GTAC'C
Cuts at: 0 1902 2136
Size: 1902 234
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 150 537 638 725 1331 1619 2136
Size: 150 387 101 87 606 288 517
Fragments arranged by size:
606 517 387 288 150 101 87
MscI TGG'CCA
Cuts at: 0 424 478 961 1111 1492 2136
Size: 424 54 483 150 381 644
Fragments arranged by size:
644 483 424 381 150 54
MslI CAynn'nnrTG
Cuts at: 0 451 1148 1201 1480 1498 1853 2136
Size: 451 697 53 279 18 355 283
Fragments arranged by size:
697 451 355 283 279 53 18
MspA1I CmG'CkG
Cuts at: 0 160 189 345 838 1659 2136
Size: 160 29 156 493 821 477
Fragments arranged by size:
821 493 477 160 156 29
NaeI GCC'GGC
Cuts at: 0 2094 2136
Size: 2094 42
NarI GG'CG_CC
Cuts at: 0 111 2136
Size: 111 2025
NcoI C'CATG_G
Cuts at: 0 1538 2136
Size: 1538 598
NgoMIV G'CCGG_C
Cuts at: 0 2092 2136
Size: 2092 44
NotI GC'GGCC_GC
Cuts at: 0 60 2136
Size: 60 2076
NspI r_CATG'y
Cuts at: 0 1177 1204 1503 2136
Size: 1177 27 299 633
Fragments arranged by size:
1177 633 299 27
PasI CC'CwG_GG
Cuts at: 0 2104 2136
Size: 2104 32
PciI A'CATG_T
Cuts at: 0 1499 2136
Size: 1499 637
PflMI CCAn_nnn'nTGG
Cuts at: 0 474 928 986 1287 1383 2104 2136
Size: 474 454 58 301 96 721 32
Fragments arranged by size:
721 474 454 301 96 58 32
PfoI T'CCnGG_A
Cuts at: 0 760 787 797 862 1580 2136
Size: 760 27 10 65 718 556
Fragments arranged by size:
760 718 556 65 27 10
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1219 1251 2136
Size: 1219 32 885
Fragments arranged by size:
1219 885 32
PpuMI rG'GwC_Cy
Cuts at: 0 177 471 550 1071 1863 2136
Size: 177 294 79 521 792 273
Fragments arranged by size:
792 521 294 273 177 79
PshAI GACnn'nnGTC
Cuts at: 0 1745 2136
Size: 1745 391
PspOMI G'GGCC_C
Cuts at: 0 278 2136
Size: 278 1858
PspXI vC'TCGA_Gb
Cuts at: 0 1225 2136
Size: 1225 911
PstI C_TGCA'G
Cuts at: 0 238 594 671 1409 2136
Size: 238 356 77 738 727
Fragments arranged by size:
738 727 356 238 77
RsrII CG'GwC_CG
Cuts at: 0 488 1683 2136
Size: 488 1195 453
Fragments arranged by size:
1195 488 453
SacI G_AGCT'C
Cuts at: 0 200 2136
Size: 200 1936
SacII CC_GC'GG
Cuts at: 0 161 190 2136
Size: 161 29 1946
Fragments arranged by size:
1946 161 29
SanDI GG'GwC_CC
Cuts at: 0 550 2136
Size: 550 1586
SbfI CC_TGCA'GG
Cuts at: 0 238 2136
Size: 238 1898
SexAI A'CCwGG_T
Cuts at: 0 767 1619 1841 2136
Size: 767 852 222 295
Fragments arranged by size:
852 767 295 222
SfcI C'TryA_G
Cuts at: 0 234 590 667 1405 2136
Size: 234 356 77 738 731
Fragments arranged by size:
738 731 356 234 77
SfoI GGC'GCC
Cuts at: 0 112 2136
Size: 112 2024
SmlI C'TyrA_G
Cuts at: 0 1225 1531 2136
Size: 1225 306 605
Fragments arranged by size:
1225 605 306
StuI AGG'CCT
Cuts at: 0 2053 2136
Size: 2053 83
StyI C'CwwG_G
Cuts at: 0 395 531 827 962 1538 2054 2136
Size: 395 136 296 135 576 516 82
Fragments arranged by size:
576 516 395 296 136 135 82
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 826 1613 1832 1865 2136
Size: 826 787 219 33 271
Fragments arranged by size:
826 787 271 219 33
TatI w'GTAC_w
Cuts at: 0 779 1349 2136
Size: 779 570 787
Fragments arranged by size:
787 779 570
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 983 1015 2136
Size: 983 32 1121
Fragments arranged by size:
1121 983 32
Tth111I GACn'n_nGTC
Cuts at: 0 1457 1843 2136
Size: 1457 386 293
Fragments arranged by size:
1457 386 293
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 303 1284 1335 2052 2136
Size: 303 981 51 717 84
Fragments arranged by size:
981 717 303 84 51
XhoI C'TCGA_G
Cuts at: 0 1225 2136
Size: 1225 911
XmnI GAAnn'nnTTC
Cuts at: 0 564 1084 2136
Size: 564 520 1052
Fragments arranged by size:
1052 564 520
Enzymes that do cut:
Acc65I AcuI AfeI AflIII AhdI AleI AlfI AloI
AlwNI ApaI ApaLI AvaI AvrII BamHI BanI BanII
BbeI BbsI BbvCI BglI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BseRI BseYI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstEII BstYI BtgI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRV FspI HaeII Hin4I HincII KasI KpnI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NotI NspI PasI PciI PflMI PfoI PpiI PpuMI
PshAI PspOMI PspXI PstI RsrII SacI SacII SanDI
SbfI SexAI SfcI SfoI SmlI StuI StyI TaqII
TatI TstI Tth111I XcmI XhoI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AflII AgeI ApoI AscI
AseI AsiSI BaeI BcgI BciVI BclI BfrBI BglII
BlpI BmtI BsaAI BsaBI BsaXI BsgI BsiWI BsmI
BspHI BssHII BstBI BstXI BstZ17I Bsu36I BtgZI BtsI
ClaI CspCI DraI EcoRI FalI FseI FspAI HindIII
HpaI MfeI MluI NdeI NheI NruI NsiI PacI
PmeI PmlI PsiI PsrI PvuI PvuII SalI SapI
ScaI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI SwaI XbaI XmaI ZraI