Restriction map for
pFN21AE2060
(Linear) (Six-base) MAPSORT of: KIEE2060.seq Check: 274 from: 1 to: 2115
KIEE2060.orf
With 182 enzymes: SgfI *
July 15, 2010 18:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1291 1422 2115
Size: 1291 131 693
Fragments arranged by size:
1291 693 131
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 224 529 2115
Size: 224 305 1586
Fragments arranged by size:
1586 305 224
AflIII A'CryG_T
Cuts at: 0 1907 2115
Size: 1907 208
AleI CACnn'nnGTG
Cuts at: 0 1573 2065 2115
Size: 1573 492 50
Fragments arranged by size:
1573 492 50
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 841 873 2115
Size: 841 32 1242
Fragments arranged by size:
1242 841 32
AlwNI CAG_nnn'CTG
Cuts at: 0 489 969 1050 2115
Size: 489 480 81 1065
Fragments arranged by size:
1065 489 480 81
ApaI G_GGCC'C
Cuts at: 0 1470 2115
Size: 1470 645
ApoI r'AATT_y
Cuts at: 0 310 2115
Size: 310 1805
AvaI C'yCGr_G
Cuts at: 0 297 2115
Size: 297 1818
BamHI G'GATC_C
Cuts at: 0 465 1487 2115
Size: 465 1022 628
Fragments arranged by size:
1022 628 465
BanI G'GyrC_C
Cuts at: 0 21 2115
Size: 21 2094
BanII G_rGCy'C
Cuts at: 0 1470 1586 2115
Size: 1470 116 529
Fragments arranged by size:
1470 529 116
BbsI GAAGACnn'nnnn_
Cuts at: 0 168 557 2115
Size: 168 389 1558
Fragments arranged by size:
1558 389 168
BciVI GTATCCnnnnn_n'
Cuts at: 0 151 1213 1523 2115
Size: 151 1062 310 592
Fragments arranged by size:
1062 592 310 151
BclI T'GATC_A
Cuts at: 0 140 2115
Size: 140 1975
BglI GCCn_nnn'nGGC
Cuts at: 0 533 2115
Size: 533 1582
BglII A'GATC_T
Cuts at: 0 230 767 2115
Size: 230 537 1348
Fragments arranged by size:
1348 537 230
Bme1580I G_kGCm'C
Cuts at: 0 928 1470 2115
Size: 928 542 645
Fragments arranged by size:
928 645 542
BmrI ACTGGGnnnn_n'
Cuts at: 0 283 295 471 2115
Size: 283 12 176 1644
Fragments arranged by size:
1644 283 176 12
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 214 600 643 730 1702 2115
Size: 214 386 43 87 972 413
Fragments arranged by size:
972 413 386 214 87 43
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 685 2115
Size: 685 1430
BsaAI yAC'GTr
Cuts at: 0 1088 2079 2115
Size: 1088 991 36
Fragments arranged by size:
1088 991 36
BsaHI Gr'CG_yC
Cuts at: 0 1943 2115
Size: 1943 172
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 249 279 2115
Size: 249 30 1836
Fragments arranged by size:
1836 249 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 382 475 1708 1813 2115
Size: 382 93 1233 105 302
Fragments arranged by size:
1233 382 302 105 93
BseYI C'CCAG_C
Cuts at: 0 470 929 1473 2000 2115
Size: 470 459 544 527 115
Fragments arranged by size:
544 527 470 459 115
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 597 679 1269 2058 2115
Size: 597 82 590 789 57
Fragments arranged by size:
789 597 590 82 57
BsiEI CG_ry'CG
Cuts at: 0 1482 2115
Size: 1482 633
BsiHKAI G_wGCw'C
Cuts at: 0 1586 2072 2115
Size: 1586 486 43
Fragments arranged by size:
1586 486 43
BsmI GAATG_Cn'
Cuts at: 0 558 1053 2115
Size: 558 495 1062
Fragments arranged by size:
1062 558 495
Bsp1286I G_dGCh'C
Cuts at: 0 928 1470 1586 2072 2115
Size: 928 542 116 486 43
Fragments arranged by size:
928 542 486 116 43
BspHI T'CATG_A
Cuts at: 0 1586 1812 2115
Size: 1586 226 303
Fragments arranged by size:
1586 303 226
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 659 977 1018 1151 1291 1422 2115
Size: 659 318 41 133 140 131 693
Fragments arranged by size:
693 659 318 140 133 131 41
BsrBI CCG'CTC
Cuts at: 0 681 2115
Size: 681 1434
BsrDI GCAATG_nn'
Cuts at: 0 846 1001 2115
Size: 846 155 1114
Fragments arranged by size:
1114 846 155
BsrFI r'CCGG_y
Cuts at: 0 24 2115
Size: 24 2091
BsrGI T'GTAC_A
Cuts at: 0 590 1277 2115
Size: 590 687 838
Fragments arranged by size:
838 687 590
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2036 2115
Size: 2036 79
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1081 1176 2115
Size: 1081 95 939
Fragments arranged by size:
1081 939 95
BstYI r'GATC_y
Cuts at: 0 230 465 767 849 1487 2115
Size: 230 235 302 82 638 628
Fragments arranged by size:
638 628 302 235 230 82
Bsu36I CC'TnA_GG
Cuts at: 0 1819 2115
Size: 1819 296
BtgI C'CryG_G
Cuts at: 0 152 1370 1449 2009 2115
Size: 152 1218 79 560 106
Fragments arranged by size:
1218 560 152 106 79
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1656 2115
Size: 1656 459
BtsI GCAGTG_nn'
Cuts at: 0 744 2115
Size: 744 1371
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1107 1142 2115
Size: 1107 35 973
Fragments arranged by size:
1107 973 35
EaeI y'GGCC_r
Cuts at: 0 2006 2115
Size: 2006 109
EarI CTCTTCn'nnn_
Cuts at: 0 1574 2115
Size: 1574 541
EcoICRI GAG'CTC
Cuts at: 0 1584 2115
Size: 1584 531
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 214 224 529 600 643 730 1702 2115
Size: 214 10 305 71 43 87 972 413
Fragments arranged by size:
972 413 305 214 87 71 43 10
EcoNI CCTnn'n_nnAGG
Cuts at: 0 203 2115
Size: 203 1912
EcoO109I rG'GnC_Cy
Cuts at: 0 684 815 1466 1467 1708 1983 2115
Size: 684 131 651 1 241 275 132
Fragments arranged by size:
684 651 275 241 132 131 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 192 2115
Size: 192 1923
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 78 110 1100 1132 1882 1914 2115
Size: 78 32 990 32 750 32 201
Fragments arranged by size:
990 750 201 78 32 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 386 486 2115
Size: 386 100 1629
Fragments arranged by size:
1629 386 100
MscI TGG'CCA
Cuts at: 0 2008 2115
Size: 2008 107
MslI CAynn'nnrTG
Cuts at: 0 673 1174 1571 1573 2065 2115
Size: 673 501 397 2 492 50
Fragments arranged by size:
673 501 492 397 50 2
MspA1I CmG'CkG
Cuts at: 0 1066 1484 1498 2115
Size: 1066 418 14 617
Fragments arranged by size:
1066 617 418 14
NaeI GCC'GGC
Cuts at: 0 26 2115
Size: 26 2089
NgoMIV G'CCGG_C
Cuts at: 0 24 2115
Size: 24 2091
NspI r_CATG'y
Cuts at: 0 1911 2115
Size: 1911 204
PasI CC'CwG_GG
Cuts at: 0 1704 2115
Size: 1704 411
PciI A'CATG_T
Cuts at: 0 1907 2115
Size: 1907 208
PflMI CCAn_nnn'nTGG
Cuts at: 0 420 1351 2115
Size: 420 931 764
Fragments arranged by size:
931 764 420
PfoI T'CCnGG_A
Cuts at: 0 442 707 2115
Size: 442 265 1408
Fragments arranged by size:
1408 442 265
PmlI CAC'GTG
Cuts at: 0 1088 2115
Size: 1088 1027
PpuMI rG'GwC_Cy
Cuts at: 0 1708 1983 2115
Size: 1708 275 132
Fragments arranged by size:
1708 275 132
PshAI GACnn'nnGTC
Cuts at: 0 1637 2115
Size: 1637 478
PspOMI G'GGCC_C
Cuts at: 0 1466 2115
Size: 1466 649
PstI C_TGCA'G
Cuts at: 0 40 974 2115
Size: 40 934 1141
Fragments arranged by size:
1141 934 40
PvuII CAG'CTG
Cuts at: 0 1066 1498 2115
Size: 1066 432 617
Fragments arranged by size:
1066 617 432
SacI G_AGCT'C
Cuts at: 0 1586 2115
Size: 1586 529
SanDI GG'GwC_CC
Cuts at: 0 1708 1983 2115
Size: 1708 275 132
Fragments arranged by size:
1708 275 132
SapI GCTCTTCn'nnn_
Cuts at: 0 1574 2115
Size: 1574 541
ScaI AGT'ACT
Cuts at: 0 1210 2115
Size: 1210 905
SexAI A'CCwGG_T
Cuts at: 0 1043 2115
Size: 1043 1072
SfcI C'TryA_G
Cuts at: 0 36 663 763 970 2115
Size: 36 627 100 207 1145
Fragments arranged by size:
1145 627 207 100 36
SmaI CCC'GGG
Cuts at: 0 299 2115
Size: 299 1816
SmlI C'TyrA_G
Cuts at: 0 700 2115
Size: 700 1415
StuI AGG'CCT
Cuts at: 0 1855 2115
Size: 1855 260
StyI C'CwwG_G
Cuts at: 0 321 1517 2115
Size: 321 1196 598
Fragments arranged by size:
1196 598 321
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 623 1940 1992 2115
Size: 623 1317 52 123
Fragments arranged by size:
1317 623 123 52
TatI w'GTAC_w
Cuts at: 0 590 741 1208 1277 2115
Size: 590 151 467 69 838
Fragments arranged by size:
838 590 467 151 69
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 149 181 249 281 1497 1529 1692 1724
Size: 149 32 68 32 1216 32 163 32
Cuts at: 1724 2115
Size: 391
Fragments arranged by size:
1216 391 163 149 68 32 32 32
32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1957 2115
Size: 1957 158
XmaI C'CCGG_G
Cuts at: 0 297 2115
Size: 297 1818
Enzymes that do cut:
AarI AcuI AflIII AleI AloI AlwNI ApaI ApoI
AvaI BamHI BanI BanII BbsI BciVI BclI BglI
BglII Bme1580I BmrI BpmI BpuEI BsaAI BsaHI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BstAPI BstXI BstYI
Bsu36I BtgI BtgZI BtsI CspCI EaeI EarI EcoICRI
Eco57MI EcoNI EcoO109I FalI Hin4I MmeI MscI MslI
MspA1I NaeI NgoMIV NspI PasI PciI PflMI PfoI
PmlI PpuMI PshAI PspOMI PstI PvuII SacI SanDI
SapI ScaI SexAI SfcI SmaI SmlI StuI StyI
TaqII TatI TstI XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AlfI ApaLI AscI AseI AsiSI AvrII BaeI BbeI
BbvCI BcgI BfrBI BlpI BmgBI BmtI BplI Bpu10I
BsaI BsaBI BsaWI BsiWI BsmBI BspEI BssHII BssSI
BstBI BstEII BstZ17I ClaI DraI DraIII DrdI EagI
EciI EcoRI EcoRV FseI FspI FspAI HaeII HincII
HindIII HpaI KasI KpnI MfeI MluI NarI NcoI
NdeI NheI NotI NruI NsiI PacI PmeI PpiI
PsiI PspXI PsrI PvuI RsrII SacII SalI SbfI
SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI
SspI SwaI Tth111I XbaI XhoI XmnI ZraI