Restriction map for
pFN21AE2058
(Linear) (Six-base) MAPSORT of: KIEE2058.seq Check: 6416 from: 1 to: 2094
KIEE2058.orf
With 182 enzymes: SgfI *
July 15, 2010 18:54 ..
AccI GT'mk_AC
Cuts at: 0 1244 2088 2094
Size: 1244 844 6
Fragments arranged by size:
1244 844 6
Acc65I G'GTAC_C
Cuts at: 0 1753 2094
Size: 1753 341
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 307 661 2094
Size: 307 354 1433
Fragments arranged by size:
1433 354 307
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1930 1962 2094
Size: 1930 32 132
Fragments arranged by size:
1930 132 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1366 1538 2094
Size: 1366 172 556
Fragments arranged by size:
1366 556 172
ApoI r'AATT_y
Cuts at: 0 898 1489 2094
Size: 898 591 605
Fragments arranged by size:
898 605 591
AvaI C'yCGr_G
Cuts at: 0 519 1902 2094
Size: 519 1383 192
Fragments arranged by size:
1383 519 192
BamHI G'GATC_C
Cuts at: 0 324 2094
Size: 324 1770
BanI G'GyrC_C
Cuts at: 0 1269 1753 2094
Size: 1269 484 341
Fragments arranged by size:
1269 484 341
BanII G_rGCy'C
Cuts at: 0 21 1558 1803 2094
Size: 21 1537 245 291
Fragments arranged by size:
1537 291 245 21
BbsI GAAGACnn'nnnn_
Cuts at: 0 1187 2094
Size: 1187 907
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 211 245 2094
Size: 211 34 1849
Fragments arranged by size:
1849 211 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1308 1588 2094
Size: 1308 280 506
Fragments arranged by size:
1308 506 280
BglI GCCn_nnn'nGGC
Cuts at: 0 214 2094
Size: 214 1880
BlpI GC'TnA_GC
Cuts at: 0 1096 2094
Size: 1096 998
Bme1580I G_kGCm'C
Cuts at: 0 534 1512 2094
Size: 534 978 582
Fragments arranged by size:
978 582 534
BmrI ACTGGGnnnn_n'
Cuts at: 0 155 1691 2094
Size: 155 1536 403
Fragments arranged by size:
1536 403 155
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1515 2094
Size: 1515 579
Bpu10I CC'TnA_GC
Cuts at: 0 476 1557 2094
Size: 476 1081 537
Fragments arranged by size:
1081 537 476
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 514 1099 2094
Size: 514 585 995
Fragments arranged by size:
995 585 514
BsaI GGTCTCn'nnnn_
Cuts at: 0 1165 2094
Size: 1165 929
BsaAI yAC'GTr
Cuts at: 0 484 2094
Size: 484 1610
BsaWI w'CCGG_w
Cuts at: 0 1025 1922 2094
Size: 1025 897 172
Fragments arranged by size:
1025 897 172
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1226 1256 2094
Size: 1226 30 838
Fragments arranged by size:
1226 838 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 908 1786 2094
Size: 908 878 308
Fragments arranged by size:
908 878 308
BseYI C'CCAG_C
Cuts at: 0 433 560 652 2094
Size: 433 127 92 1442
Fragments arranged by size:
1442 433 127 92
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 104 2061 2094
Size: 104 1957 33
Fragments arranged by size:
1957 104 33
BsiEI CG_ry'CG
Cuts at: 0 1927 2094
Size: 1927 167
BsmI GAATG_Cn'
Cuts at: 0 1075 2094
Size: 1075 1019
Bsp1286I G_dGCh'C
Cuts at: 0 21 534 1512 1558 1803 2094
Size: 21 513 978 46 245 291
Fragments arranged by size:
978 513 291 245 46 21
BspEI T'CCGG_A
Cuts at: 0 1025 2094
Size: 1025 1069
BspHI T'CATG_A
Cuts at: 0 1199 1980 2094
Size: 1199 781 114
Fragments arranged by size:
1199 781 114
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 238 416 941 2094
Size: 238 178 525 1153
Fragments arranged by size:
1153 525 238 178
BsrDI GCAATG_nn'
Cuts at: 0 209 2094
Size: 209 1885
BsrFI r'CCGG_y
Cuts at: 0 756 2094
Size: 756 1338
BssSI C'ACGA_G
Cuts at: 0 1105 2094
Size: 1105 989
BstEII G'GTnAC_C
Cuts at: 0 2035 2094
Size: 2035 59
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1227 2094
Size: 1227 867
BstYI r'GATC_y
Cuts at: 0 324 2094
Size: 324 1770
BtgI C'CryG_G
Cuts at: 0 11 954 1445 1702 2094
Size: 11 943 491 257 392
Fragments arranged by size:
943 491 392 257 11
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1173 2094
Size: 1173 921
BtsI GCAGTG_nn'
Cuts at: 0 92 509 1016 1352 1473 2094
Size: 92 417 507 336 121 621
Fragments arranged by size:
621 507 417 336 121 92
DraI TTT'AAA
Cuts at: 0 965 2094
Size: 965 1129
DraIII CAC_nnn'GTG
Cuts at: 0 110 1815 2094
Size: 110 1705 279
Fragments arranged by size:
1705 279 110
EaeI y'GGCC_r
Cuts at: 0 506 1793 1970 2094
Size: 506 1287 177 124
Fragments arranged by size:
1287 506 177 124
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 307 661 1515 2094
Size: 307 354 854 579
Fragments arranged by size:
854 579 354 307
EcoO109I rG'GnC_Cy
Cuts at: 0 792 930 1938 2094
Size: 792 138 1008 156
Fragments arranged by size:
1008 792 156 138
EcoRI G'AATT_C
Cuts at: 0 898 2094
Size: 898 1196
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 855 2094
Size: 855 1239
HaeII r_GCGC'y
Cuts at: 0 1616 2094
Size: 1616 478
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1147 1179 1226 1258 1845 1877 2094
Size: 1147 32 47 32 587 32 217
Fragments arranged by size:
1147 587 217 47 32 32 32
HincII GTy'rAC
Cuts at: 0 571 1137 1864 2094
Size: 571 566 727 230
Fragments arranged by size:
727 571 566 230
HindIII A'AGCT_T
Cuts at: 0 1838 2094
Size: 1838 256
HpaI GTT'AAC
Cuts at: 0 1137 2094
Size: 1137 957
KpnI G_GTAC'C
Cuts at: 0 1757 2094
Size: 1757 337
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 71 927 1907 2094
Size: 71 856 980 187
Fragments arranged by size:
980 856 187 71
MscI TGG'CCA
Cuts at: 0 508 1795 1972 2094
Size: 508 1287 177 122
Fragments arranged by size:
1287 508 177 122
MslI CAynn'nnrTG
Cuts at: 0 1444 2094
Size: 1444 650
MspA1I CmG'CkG
Cuts at: 0 433 527 564 1429 2094
Size: 433 94 37 865 665
Fragments arranged by size:
865 665 433 94 37
NcoI C'CATG_G
Cuts at: 0 1445 1702 2094
Size: 1445 257 392
Fragments arranged by size:
1445 392 257
NdeI CA'TA_TG
Cuts at: 0 131 1995 2094
Size: 131 1864 99
Fragments arranged by size:
1864 131 99
NspI r_CATG'y
Cuts at: 0 1049 2094
Size: 1049 1045
PasI CC'CwG_GG
Cuts at: 0 1513 2094
Size: 1513 581
PflMI CCAn_nnn'nTGG
Cuts at: 0 154 586 2094
Size: 154 432 1508
Fragments arranged by size:
1508 432 154
PfoI T'CCnGG_A
Cuts at: 0 2066 2094
Size: 2066 28
PpuMI rG'GwC_Cy
Cuts at: 0 792 930 1938 2094
Size: 792 138 1008 156
Fragments arranged by size:
1008 792 156 138
PshAI GACnn'nnGTC
Cuts at: 0 1532 2094
Size: 1532 562
PstI C_TGCA'G
Cuts at: 0 1480 2094
Size: 1480 614
PvuII CAG'CTG
Cuts at: 0 527 564 2094
Size: 527 37 1530
Fragments arranged by size:
1530 527 37
SfcI C'TryA_G
Cuts at: 0 1340 1476 2094
Size: 1340 136 618
Fragments arranged by size:
1340 618 136
SmlI C'TyrA_G
Cuts at: 0 493 1114 2094
Size: 493 621 980
Fragments arranged by size:
980 621 493
StuI AGG'CCT
Cuts at: 0 1562 2094
Size: 1562 532
StyI C'CwwG_G
Cuts at: 0 1445 1702 1934 2094
Size: 1445 257 232 160
Fragments arranged by size:
1445 257 232 160
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 425 2094
Size: 425 1669
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 874 996 1414 1764 2094
Size: 874 122 418 350 330
Fragments arranged by size:
874 418 350 330 122
Enzymes that do cut:
AccI Acc65I AcuI AloI AlwNI ApoI AvaI BamHI
BanI BanII BbsI BcgI BciVI BglI BlpI Bme1580I
BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsmI Bsp1286I BspEI BspHI
BspMI BsrDI BsrFI BssSI BstEII BstXI BstYI BtgI
BtgZI BtsI DraI DraIII EaeI Eco57MI EcoO109I EcoRI
FalI HaeII Hin4I HincII HindIII HpaI KpnI MmeI
MscI MslI MspA1I NcoI NdeI NspI PasI PflMI
PfoI PpuMI PshAI PstI PvuII SfcI SmlI StuI
StyI TaqII XcmI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI ApaI ApaLI AscI AseI AsiSI AvrII
BaeI BbeI BbvCI BclI BfrBI BglII BmgBI BmtI
BplI BsaBI BsaHI BsiHKAI BsiWI BsmBI BsrBI BsrGI
BssHII BstAPI BstBI BstZ17I Bsu36I ClaI CspCI DrdI
EagI EarI EciI EcoICRI EcoNI EcoRV FseI FspI
FspAI KasI MfeI MluI NaeI NarI NgoMIV NheI
NotI NruI NsiI PacI PciI PmeI PmlI PpiI
PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
SwaI TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI