Restriction map for
pFN21AE2057
(Linear) (Six-base) MAPSORT of: KIEE2057.seq Check: 1105 from: 1 to: 2079
KIEE2057.orf
With 182 enzymes: SgfI *
July 15, 2010 18:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1041 2079
Size: 1041 1038
AatII G_ACGT'C
Cuts at: 0 1307 1408 2079
Size: 1307 101 671
Fragments arranged by size:
1307 671 101
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 247 777 1480 1711 1945 2079
Size: 247 530 703 231 234 134
Fragments arranged by size:
703 530 247 234 231 134
AfeI AGC'GCT
Cuts at: 0 848 2079
Size: 848 1231
AgeI A'CCGG_T
Cuts at: 0 724 2079
Size: 724 1355
AleI CACnn'nnGTG
Cuts at: 0 1720 2079
Size: 1720 359
AlwNI CAG_nnn'CTG
Cuts at: 0 563 2079
Size: 563 1516
ApaI G_GGCC'C
Cuts at: 0 1033 1952 2079
Size: 1033 919 127
Fragments arranged by size:
1033 919 127
ApoI r'AATT_y
Cuts at: 0 591 984 1233 2079
Size: 591 393 249 846
Fragments arranged by size:
846 591 393 249
AvaI C'yCGr_G
Cuts at: 0 872 1964 2079
Size: 872 1092 115
Fragments arranged by size:
1092 872 115
AvrII C'CTAG_G
Cuts at: 0 1944 2079
Size: 1944 135
BanI G'GyrC_C
Cuts at: 0 1999 2079
Size: 1999 80
BanII G_rGCy'C
Cuts at: 0 89 1033 1442 1952 2079
Size: 89 944 409 510 127
Fragments arranged by size:
944 510 409 127 89
BbsI GAAGACnn'nnnn_
Cuts at: 0 554 1400 1543 2079
Size: 554 846 143 536
Fragments arranged by size:
846 554 536 143
BciVI GTATCCnnnnn_n'
Cuts at: 0 1524 2079
Size: 1524 555
BclI T'GATC_A
Cuts at: 0 125 668 878 2079
Size: 125 543 210 1201
Fragments arranged by size:
1201 543 210 125
BglII A'GATC_T
Cuts at: 0 347 1661 2079
Size: 347 1314 418
Fragments arranged by size:
1314 418 347
Bme1580I G_kGCm'C
Cuts at: 0 1033 1952 2079
Size: 1033 919 127
Fragments arranged by size:
1033 919 127
BmgBI CAC'GTC
Cuts at: 0 1935 2079
Size: 1935 144
BmrI ACTGGGnnnn_n'
Cuts at: 0 793 2079
Size: 793 1286
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 60 181 270 285 328 1816 2079
Size: 60 121 89 15 43 1488 263
Fragments arranged by size:
1488 263 121 89 60 43 15
Bpu10I CC'TnA_GC
Cuts at: 0 1522 2079
Size: 1522 557
BsaBI GATnn'nnATC
Cuts at: 0 1711 2079
Size: 1711 368
BsaHI Gr'CG_yC
Cuts at: 0 1304 1405 2079
Size: 1304 101 674
Fragments arranged by size:
1304 674 101
BsaWI w'CCGG_w
Cuts at: 0 724 1709 2079
Size: 724 985 370
Fragments arranged by size:
985 724 370
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 58 88 2079
Size: 58 30 1991
Fragments arranged by size:
1991 58 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 360 2079
Size: 360 1719
BseYI C'CCAG_C
Cuts at: 0 700 818 829 1441 2079
Size: 700 118 11 612 638
Fragments arranged by size:
700 638 612 118 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1159 1858 2079
Size: 1159 699 221
Fragments arranged by size:
1159 699 221
BsiEI CG_ry'CG
Cuts at: 0 1188 2079
Size: 1188 891
BsiHKAI G_wGCw'C
Cuts at: 0 89 2079
Size: 89 1990
BsiWI C'GTAC_G
Cuts at: 0 1037 1667 2079
Size: 1037 630 412
Fragments arranged by size:
1037 630 412
Bsp1286I G_dGCh'C
Cuts at: 0 89 1033 1442 1952 2079
Size: 89 944 409 510 127
Fragments arranged by size:
944 510 409 127 89
BspEI T'CCGG_A
Cuts at: 0 1709 2079
Size: 1709 370
BspHI T'CATG_A
Cuts at: 0 128 900 2079
Size: 128 772 1179
Fragments arranged by size:
1179 772 128
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1041 1250 2079
Size: 1041 209 829
Fragments arranged by size:
1041 829 209
BsrBI CCG'CTC
Cuts at: 0 713 1761 2079
Size: 713 1048 318
Fragments arranged by size:
1048 713 318
BsrDI GCAATG_nn'
Cuts at: 0 279 684 1296 2079
Size: 279 405 612 783
Fragments arranged by size:
783 612 405 279
BsrFI r'CCGG_y
Cuts at: 0 478 724 1869 2079
Size: 478 246 1145 210
Fragments arranged by size:
1145 478 246 210
BsrGI T'GTAC_A
Cuts at: 0 1590 2079
Size: 1590 489
BssSI C'ACGA_G
Cuts at: 0 1814 2079
Size: 1814 265
BstEII G'GTnAC_C
Cuts at: 0 439 1918 2079
Size: 439 1479 161
Fragments arranged by size:
1479 439 161
BstXI CCAn_nnnn'nTGG
Cuts at: 0 746 1281 1860 2079
Size: 746 535 579 219
Fragments arranged by size:
746 579 535 219
BstYI r'GATC_y
Cuts at: 0 347 764 1661 2079
Size: 347 417 897 418
Fragments arranged by size:
897 418 417 347
Bsu36I CC'TnA_GG
Cuts at: 0 1634 2079
Size: 1634 445
BtgI C'CryG_G
Cuts at: 0 944 2079
Size: 944 1135
ClaI AT'CG_AT
Cuts at: 0 2066 2079
Size: 2066 13
EaeI y'GGCC_r
Cuts at: 0 200 620 648 729 810 2079
Size: 200 420 28 81 81 1269
Fragments arranged by size:
1269 420 200 81 81 28
EarI CTCTTCn'nnn_
Cuts at: 0 452 1634 1852 2079
Size: 452 1182 218 227
Fragments arranged by size:
1182 452 227 218
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 586 2079
Size: 586 1493
EcoICRI GAG'CTC
Cuts at: 0 87 2079
Size: 87 1992
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 60 181 247 270 285 328 777 1480
Size: 60 121 66 23 15 43 449 703
Cuts at: 1480 1711 1816 1945 2079
Size: 231 105 129 134
Fragments arranged by size:
703 449 231 134 129 121 105 66
60 43 23 15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 961 2079
Size: 961 1118
EcoO109I rG'GnC_Cy
Cuts at: 0 181 735 833 1030 1731 1948 1961 2079
Size: 181 554 98 197 701 217 13 118
Fragments arranged by size:
701 554 217 197 181 118 98 13
EcoRI G'AATT_C
Cuts at: 0 1233 2079
Size: 1233 846
EcoRV GAT'ATC
Cuts at: 0 1980 2079
Size: 1980 99
HaeII r_GCGC'y
Cuts at: 0 850 2079
Size: 850 1229
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 58 90 2079
Size: 58 32 1989
Fragments arranged by size:
1989 58 32
HincII GTy'rAC
Cuts at: 0 1497 2079
Size: 1497 582
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1037 2079
Size: 1037 1042
MscI TGG'CCA
Cuts at: 0 622 812 2079
Size: 622 190 1267
Fragments arranged by size:
1267 622 190
MslI CAynn'nnrTG
Cuts at: 0 490 1720 2079
Size: 490 1230 359
Fragments arranged by size:
1230 490 359
MspA1I CmG'CkG
Cuts at: 0 1143 1720 2079
Size: 1143 577 359
Fragments arranged by size:
1143 577 359
NaeI GCC'GGC
Cuts at: 0 480 2079
Size: 480 1599
NcoI C'CATG_G
Cuts at: 0 944 2079
Size: 944 1135
NgoMIV G'CCGG_C
Cuts at: 0 478 2079
Size: 478 1601
NspI r_CATG'y
Cuts at: 0 1158 1476 2079
Size: 1158 318 603
Fragments arranged by size:
1158 603 318
PasI CC'CwG_GG
Cuts at: 0 357 2079
Size: 357 1722
PfoI T'CCnGG_A
Cuts at: 0 1772 1990 2079
Size: 1772 218 89
Fragments arranged by size:
1772 218 89
PpuMI rG'GwC_Cy
Cuts at: 0 735 833 1731 1961 2079
Size: 735 98 898 230 118
Fragments arranged by size:
898 735 230 118 98
PspOMI G'GGCC_C
Cuts at: 0 1029 1948 2079
Size: 1029 919 131
Fragments arranged by size:
1029 919 131
PvuII CAG'CTG
Cuts at: 0 1143 2079
Size: 1143 936
SacI G_AGCT'C
Cuts at: 0 89 2079
Size: 89 1990
SanDI GG'GwC_CC
Cuts at: 0 735 833 1961 2079
Size: 735 98 1128 118
Fragments arranged by size:
1128 735 118 98
SapI GCTCTTCn'nnn_
Cuts at: 0 1852 2079
Size: 1852 227
SexAI A'CCwGG_T
Cuts at: 0 1499 2079
Size: 1499 580
SgrAI Cr'CCGG_yG
Cuts at: 0 724 2079
Size: 724 1355
SmaI CCC'GGG
Cuts at: 0 874 1966 2079
Size: 874 1092 113
Fragments arranged by size:
1092 874 113
SphI G_CATG'C
Cuts at: 0 1476 2079
Size: 1476 603
StuI AGG'CCT
Cuts at: 0 40 140 2079
Size: 40 100 1939
Fragments arranged by size:
1939 100 40
StyI C'CwwG_G
Cuts at: 0 141 739 944 1083 1274 1944 1952 2079
Size: 141 598 205 139 191 670 8 127
Fragments arranged by size:
670 598 205 191 141 139 127 8
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 333 926 1101 2079
Size: 333 593 175 978
Fragments arranged by size:
978 593 333 175
TatI w'GTAC_w
Cuts at: 0 932 1316 1590 2079
Size: 932 384 274 489
Fragments arranged by size:
932 489 384 274
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1037 1069 2079
Size: 1037 32 1010
Fragments arranged by size:
1037 1010 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1623 1791 2079
Size: 1623 168 288
Fragments arranged by size:
1623 288 168
XmaI C'CCGG_G
Cuts at: 0 872 1964 2079
Size: 872 1092 115
Fragments arranged by size:
1092 872 115
XmnI GAAnn'nnTTC
Cuts at: 0 40 551 2079
Size: 40 511 1528
Fragments arranged by size:
1528 511 40
ZraI GAC'GTC
Cuts at: 0 1305 1406 2079
Size: 1305 101 673
Fragments arranged by size:
1305 673 101
Enzymes that do cut:
AarI AatII AcuI AfeI AgeI AleI AlwNI ApaI
ApoI AvaI AvrII BanI BanII BbsI BciVI BclI
BglII Bme1580I BmgBI BmrI BpmI Bpu10I BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstEII BstXI BstYI Bsu36I BtgI ClaI EaeI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
HaeII Hin4I HincII MmeI MscI MslI MspA1I NaeI
NcoI NgoMIV NspI PasI PfoI PpuMI PspOMI PvuII
SacI SanDI SapI SexAI SgrAI SmaI SphI StuI
StyI TaqII TatI TstI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AflII AflIII AhdI AlfI AloI
ApaLI AscI AseI AsiSI BaeI BamHI BbeI BbvCI
BcgI BfrBI BglI BlpI BmtI BplI BpuEI BsaI
BsaAI BsmI BsmBI BssHII BstAPI BstBI BstZ17I BtgZI
BtsI CspCI DraI DraIII DrdI EagI FalI FseI
FspI FspAI HindIII HpaI KasI KpnI MfeI MluI
NarI NdeI NheI NotI NruI NsiI PacI PciI
PflMI PmeI PmlI PpiI PshAI PsiI PspXI PsrI
PstI PvuI RsrII SacII SalI SbfI ScaI SfcI
SfiI SfoI SgfI SmlI SnaBI SpeI SrfI SspI
SwaI Tth111I XbaI XhoI