Restriction map for
pFN21AE2056
(Linear) (Six-base) MAPSORT of: KIEE2056.seq Check: 7994 from: 1 to: 2076
KIEE2056.orf
With 182 enzymes: SgfI *
July 3, 2012 17:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1502 1872 2076
Size: 1502 370 204
Fragments arranged by size:
1502 370 204
AatII G_ACGT'C
Cuts at: 0 1730 2076
Size: 1730 346
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2048 2076
Size: 2048 28
AhdI GACnn_n'nnGTC
Cuts at: 0 306 474 2076
Size: 306 168 1602
Fragments arranged by size:
1602 306 168
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 628 1288 2076
Size: 628 660 788
Fragments arranged by size:
788 660 628
AlwNI CAG_nnn'CTG
Cuts at: 0 413 1872 1945 2076
Size: 413 1459 73 131
Fragments arranged by size:
1459 413 131 73
ApaI G_GGCC'C
Cuts at: 0 216 713 2076
Size: 216 497 1363
Fragments arranged by size:
1363 497 216
ApoI r'AATT_y
Cuts at: 0 1027 1160 1234 2076
Size: 1027 133 74 842
Fragments arranged by size:
1027 842 133 74
AvaI C'yCGr_G
Cuts at: 0 530 956 2076
Size: 530 426 1120
Fragments arranged by size:
1120 530 426
BanI G'GyrC_C
Cuts at: 0 115 604 1490 1884 1954 2076
Size: 115 489 886 394 70 122
Fragments arranged by size:
886 489 394 122 115 70
BanII G_rGCy'C
Cuts at: 0 216 373 544 656 713 2076
Size: 216 157 171 112 57 1363
Fragments arranged by size:
1363 216 171 157 112 57
BbeI G_GCGC'C
Cuts at: 0 119 1958 2076
Size: 119 1839 118
Fragments arranged by size:
1839 119 118
BbvCI CC'TCA_GC
Cuts at: 0 1811 2076
Size: 1811 265
BglI GCCn_nnn'nGGC
Cuts at: 0 184 2076
Size: 184 1892
Bme1580I G_kGCm'C
Cuts at: 0 216 635 713 1889 1989 2076
Size: 216 419 78 1176 100 87
Fragments arranged by size:
1176 419 216 100 87 78
BmrI ACTGGGnnnn_n'
Cuts at: 0 741 2076
Size: 741 1335
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 558 2076
Size: 558 1518
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 390 1282 1468 1772 2076
Size: 390 892 186 304 304
Fragments arranged by size:
892 390 304 304 186
Bpu10I CC'TnA_GC
Cuts at: 0 1014 1811 2076
Size: 1014 797 265
Fragments arranged by size:
1014 797 265
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1603 1841 2076
Size: 1603 238 235
Fragments arranged by size:
1603 238 235
BsaI GGTCTCn'nnnn_
Cuts at: 0 515 1240 2076
Size: 515 725 836
Fragments arranged by size:
836 725 515
BsaAI yAC'GTr
Cuts at: 0 687 2076
Size: 687 1389
BsaBI GATnn'nnATC
Cuts at: 0 854 2076
Size: 854 1222
BsaHI Gr'CG_yC
Cuts at: 0 116 1341 1727 1913 1955 2076
Size: 116 1225 386 186 42 121
Fragments arranged by size:
1225 386 186 121 116 42
BsaWI w'CCGG_w
Cuts at: 0 590 2076
Size: 590 1486
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1483 1513 2076
Size: 1483 30 563
Fragments arranged by size:
1483 563 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 882 949 1085 1471 1726 2076
Size: 882 67 136 386 255 350
Fragments arranged by size:
882 386 350 255 136 67
BseYI C'CCAG_C
Cuts at: 0 162 442 525 718 2010 2076
Size: 162 280 83 193 1292 66
Fragments arranged by size:
1292 280 193 162 83 66
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1169 1451 1480 2076
Size: 1169 282 29 596
Fragments arranged by size:
1169 596 282 29
BsiEI CG_ry'CG
Cuts at: 0 209 483 2076
Size: 209 274 1593
Fragments arranged by size:
1593 274 209
BsiHKAI G_wGCw'C
Cuts at: 0 373 428 1412 2076
Size: 373 55 984 664
Fragments arranged by size:
984 664 373 55
BsmI GAATG_Cn'
Cuts at: 0 520 2076
Size: 520 1556
Bsp1286I G_dGCh'C
Cuts at: 0 216 373 428 544 635 656 713 1412
Size: 216 157 55 116 91 21 57 699
Cuts at: 1412 1889 1989 2076
Size: 477 100 87
Fragments arranged by size:
699 477 216 157 116 100 91 87
57 55 21
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 51 1502 1514 1872 2076
Size: 51 1451 12 358 204
Fragments arranged by size:
1451 358 204 51 12
BsrBI CCG'CTC
Cuts at: 0 335 537 830 1013 1786 2076
Size: 335 202 293 183 773 290
Fragments arranged by size:
773 335 293 290 202 183
BsrDI GCAATG_nn'
Cuts at: 0 275 492 2076
Size: 275 217 1584
Fragments arranged by size:
1584 275 217
BsrFI r'CCGG_y
Cuts at: 0 722 1806 1843 2076
Size: 722 1084 37 233
Fragments arranged by size:
1084 722 233 37
BssSI C'ACGA_G
Cuts at: 0 168 2076
Size: 168 1908
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1984 2076
Size: 1984 92
BstXI CCAn_nnnn'nTGG
Cuts at: 0 170 228 2076
Size: 170 58 1848
Fragments arranged by size:
1848 170 58
BstYI r'GATC_y
Cuts at: 0 1211 1383 2076
Size: 1211 172 693
Fragments arranged by size:
1211 693 172
Bsu36I CC'TnA_GG
Cuts at: 0 1709 2076
Size: 1709 367
BtgI C'CryG_G
Cuts at: 0 752 1286 1502 1721 1826 2018 2076
Size: 752 534 216 219 105 192 58
Fragments arranged by size:
752 534 219 216 192 105 58
BtsI GCAGTG_nn'
Cuts at: 0 1437 1760 2076
Size: 1437 323 316
Fragments arranged by size:
1437 323 316
DraIII CAC_nnn'GTG
Cuts at: 0 1503 2076
Size: 1503 573
EaeI y'GGCC_r
Cuts at: 0 77 206 724 825 1401 1718 2076
Size: 77 129 518 101 576 317 358
Fragments arranged by size:
576 518 358 317 129 101 77
EagI C'GGCC_G
Cuts at: 0 206 2076
Size: 206 1870
EarI CTCTTCn'nnn_
Cuts at: 0 745 1099 1122 1202 1871 2076
Size: 745 354 23 80 669 205
Fragments arranged by size:
745 669 354 205 80 23
EcoICRI GAG'CTC
Cuts at: 0 371 2076
Size: 371 1705
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 390 1282 1468 1772 2048 2076
Size: 390 892 186 304 276 28
Fragments arranged by size:
892 390 304 276 186 28
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1391 2076
Size: 1391 685
EcoO109I rG'GnC_Cy
Cuts at: 0 212 213 410 699 709 710 1629 1822
Size: 212 1 197 289 10 1 919 193
Cuts at: 1822 2076
Size: 254
Fragments arranged by size:
919 289 254 212 197 193 10 1
1
EcoRI G'AATT_C
Cuts at: 0 1027 1160 2076
Size: 1027 133 916
Fragments arranged by size:
1027 916 133
FspI TGC'GCA
Cuts at: 0 616 2076
Size: 616 1460
HaeII r_GCGC'y
Cuts at: 0 119 1783 1958 2076
Size: 119 1664 175 118
Fragments arranged by size:
1664 175 119 118
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 292 324 460 492 643 675 834 866
Size: 292 32 136 32 151 32 159 32
Cuts at: 866 997 1029 2035 2067 2076
Size: 131 32 1006 32 9
Fragments arranged by size:
1006 292 159 151 136 131 32 32
32 32 32 32 9
HincII GTy'rAC
Cuts at: 0 963 2076
Size: 963 1113
KasI G'GCGC_C
Cuts at: 0 115 1954 2076
Size: 115 1839 122
Fragments arranged by size:
1839 122 115
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 290 503 948 2076
Size: 290 213 445 1128
Fragments arranged by size:
1128 445 290 213
MscI TGG'CCA
Cuts at: 0 79 1403 2076
Size: 79 1324 673
Fragments arranged by size:
1324 673 79
MslI CAynn'nnrTG
Cuts at: 0 226 610 2076
Size: 226 384 1466
Fragments arranged by size:
1466 384 226
MspA1I CmG'CkG
Cuts at: 0 83 640 1000 1488 1723 1799 1837 1869
Size: 83 557 360 488 235 76 38 32
Cuts at: 1869 2014 2020 2076
Size: 145 6 56
Fragments arranged by size:
557 488 360 235 145 83 76 56
38 32 6
NaeI GCC'GGC
Cuts at: 0 724 1808 2076
Size: 724 1084 268
Fragments arranged by size:
1084 724 268
NarI GG'CG_CC
Cuts at: 0 116 1955 2076
Size: 116 1839 121
Fragments arranged by size:
1839 121 116
NcoI C'CATG_G
Cuts at: 0 752 2076
Size: 752 1324
NdeI CA'TA_TG
Cuts at: 0 63 2076
Size: 63 2013
NgoMIV G'CCGG_C
Cuts at: 0 722 1806 2076
Size: 722 1084 270
Fragments arranged by size:
1084 722 270
NspI r_CATG'y
Cuts at: 0 783 1458 2076
Size: 783 675 618
Fragments arranged by size:
783 675 618
PasI CC'CwG_GG
Cuts at: 0 54 2076
Size: 54 2022
PflMI CCAn_nnn'nTGG
Cuts at: 0 297 668 753 2076
Size: 297 371 85 1323
Fragments arranged by size:
1323 371 297 85
PfoI T'CCnGG_A
Cuts at: 0 584 1298 2076
Size: 584 714 778
Fragments arranged by size:
778 714 584
PpuMI rG'GwC_Cy
Cuts at: 0 1629 2076
Size: 1629 447
PspOMI G'GGCC_C
Cuts at: 0 212 709 2076
Size: 212 497 1367
Fragments arranged by size:
1367 497 212
PstI C_TGCA'G
Cuts at: 0 1941 2076
Size: 1941 135
PvuII CAG'CTG
Cuts at: 0 83 1000 1869 2014 2076
Size: 83 917 869 145 62
Fragments arranged by size:
917 869 145 83 62
SacI G_AGCT'C
Cuts at: 0 373 2076
Size: 373 1703
SacII CC_GC'GG
Cuts at: 0 1724 2021 2076
Size: 1724 297 55
Fragments arranged by size:
1724 297 55
SapI GCTCTTCn'nnn_
Cuts at: 0 1871 2076
Size: 1871 205
SfcI C'TryA_G
Cuts at: 0 150 447 1937 2076
Size: 150 297 1490 139
Fragments arranged by size:
1490 297 150 139
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 184 2076
Size: 184 1892
SfoI GGC'GCC
Cuts at: 0 117 1956 2076
Size: 117 1839 120
Fragments arranged by size:
1839 120 117
SmaI CCC'GGG
Cuts at: 0 532 958 2076
Size: 532 426 1118
Fragments arranged by size:
1118 532 426
SmlI C'TyrA_G
Cuts at: 0 1618 1856 2076
Size: 1618 238 220
Fragments arranged by size:
1618 238 220
SphI G_CATG'C
Cuts at: 0 783 2076
Size: 783 1293
StyI C'CwwG_G
Cuts at: 0 182 311 752 1919 2076
Size: 182 129 441 1167 157
Fragments arranged by size:
1167 441 182 157 129
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 67 679 2076
Size: 67 612 1397
Fragments arranged by size:
1397 612 67
TatI w'GTAC_w
Cuts at: 0 506 2076
Size: 506 1570
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 59 91 1483 1515 2076
Size: 59 32 1392 32 561
Fragments arranged by size:
1392 561 59 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 61 1560 2076
Size: 61 1499 516
Fragments arranged by size:
1499 516 61
XmaI C'CCGG_G
Cuts at: 0 530 956 2076
Size: 530 426 1120
Fragments arranged by size:
1120 530 426
XmnI GAAnn'nnTTC
Cuts at: 0 919 2076
Size: 919 1157
ZraI GAC'GTC
Cuts at: 0 1728 2076
Size: 1728 348
Enzymes that do cut:
AarI AatII AcuI AhdI AlfI AlwNI ApaI ApoI
AvaI BanI BanII BbeI BbvCI BglI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstXI
BstYI Bsu36I BtgI BtsI DraIII EaeI EagI EarI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII KasI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NspI PasI PflMI PfoI PpuMI
PspOMI PstI PvuII SacI SacII SapI SfcI SfiI
SfoI SmaI SmlI SphI StyI TaqII TatI TstI
XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AfeI AflII AflIII AgeI AleI
AloI ApaLI AscI AseI AsiSI AvrII BaeI BamHI
BbsI BcgI BciVI BclI BfrBI BglII BlpI BmgBI
BmtI BsiWI BsmBI BspEI BspHI BsrGI BssHII BstBI
BstEII BstZ17I BtgZI ClaI CspCI DraI DrdI EciI
EcoRV FalI FseI FspAI HindIII HpaI KpnI MfeI
MluI NheI NotI NruI NsiI PacI PciI PmeI
PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII
SalI SanDI SbfI ScaI SexAI SgfI SgrAI SnaBI
SpeI SrfI SspI StuI SwaI Tth111I XbaI XhoI