Restriction map for
pFN21AE2053
(Linear) (Six-base) MAPSORT of: KIEE2053.seq Check: 7175 from: 1 to: 2064
KIEE2053.orf
With 182 enzymes: SgfI *
July 15, 2010 18:53 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 155 251 2064
Size: 155 96 1813
Fragments arranged by size:
1813 155 96
AfeI AGC'GCT
Cuts at: 0 1388 2064
Size: 1388 676
AflIII A'CryG_T
Cuts at: 0 990 2064
Size: 990 1074
AhdI GACnn_n'nnGTC
Cuts at: 0 1630 2064
Size: 1630 434
AleI CACnn'nnGTG
Cuts at: 0 1471 2064
Size: 1471 593
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 616 648 2064
Size: 616 32 1416
Fragments arranged by size:
1416 616 32
ApaI G_GGCC'C
Cuts at: 0 1246 1970 2064
Size: 1246 724 94
Fragments arranged by size:
1246 724 94
ApoI r'AATT_y
Cuts at: 0 1936 2064
Size: 1936 128
AseI AT'TA_AT
Cuts at: 0 1394 2064
Size: 1394 670
BamHI G'GATC_C
Cuts at: 0 1321 1917 2064
Size: 1321 596 147
Fragments arranged by size:
1321 596 147
BanI G'GyrC_C
Cuts at: 0 1654 2064
Size: 1654 410
BanII G_rGCy'C
Cuts at: 0 1246 1970 1991 2064
Size: 1246 724 21 73
Fragments arranged by size:
1246 724 73 21
BbsI GAAGACnn'nnnn_
Cuts at: 0 896 1118 1350 2035 2064
Size: 896 222 232 685 29
Fragments arranged by size:
896 685 232 222 29
BglI GCCn_nnn'nGGC
Cuts at: 0 1652 2064
Size: 1652 412
BlpI GC'TnA_GC
Cuts at: 0 44 2064
Size: 44 2020
Bme1580I G_kGCm'C
Cuts at: 0 1246 1970 2064
Size: 1246 724 94
Fragments arranged by size:
1246 724 94
BmgBI CAC'GTC
Cuts at: 0 991 2064
Size: 991 1073
BmrI ACTGGGnnnn_n'
Cuts at: 0 92 1413 1606 1965 2064
Size: 92 1321 193 359 99
Fragments arranged by size:
1321 359 193 99 92
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 815 2064
Size: 815 1249
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 301 1877 2064
Size: 301 1576 187
Fragments arranged by size:
1576 301 187
Bpu10I CC'TnA_GC
Cuts at: 0 1514 2064
Size: 1514 550
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 582 1729 2064
Size: 582 1147 335
Fragments arranged by size:
1147 582 335
BsaWI w'CCGG_w
Cuts at: 0 617 2064
Size: 617 1447
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 55 85 231 261 451 481 616 646
Size: 55 30 146 30 190 30 135 30
Cuts at: 646 1620 1650 2064
Size: 974 30 414
Fragments arranged by size:
974 414 190 146 135 55 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 270 1174 1202 1874 2064
Size: 270 904 28 672 190
Fragments arranged by size:
904 672 270 190 28
BseYI C'CCAG_C
Cuts at: 0 1223 1239 1706 2064
Size: 1223 16 467 358
Fragments arranged by size:
1223 467 358 16
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 229 527 970 1495 2064
Size: 229 298 443 525 569
Fragments arranged by size:
569 525 443 298 229
BsiHKAI G_wGCw'C
Cuts at: 0 1126 2064
Size: 1126 938
BsmI GAATG_Cn'
Cuts at: 0 406 1079 2064
Size: 406 673 985
Fragments arranged by size:
985 673 406
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1638 2064
Size: 1638 426
Bsp1286I G_dGCh'C
Cuts at: 0 1126 1246 1970 1991 2064
Size: 1126 120 724 21 73
Fragments arranged by size:
1126 724 120 73 21
BsrDI GCAATG_nn'
Cuts at: 0 1035 1586 2064
Size: 1035 551 478
Fragments arranged by size:
1035 551 478
BsrGI T'GTAC_A
Cuts at: 0 1911 2064
Size: 1911 153
BstEII G'GTnAC_C
Cuts at: 0 645 1757 2064
Size: 645 1112 307
Fragments arranged by size:
1112 645 307
BstYI r'GATC_y
Cuts at: 0 65 614 867 1321 1917 2064
Size: 65 549 253 454 596 147
Fragments arranged by size:
596 549 454 253 147 65
Bsu36I CC'TnA_GG
Cuts at: 0 86 884 2064
Size: 86 798 1180
Fragments arranged by size:
1180 798 86
BtgI C'CryG_G
Cuts at: 0 1372 2064
Size: 1372 692
BtsI GCAGTG_nn'
Cuts at: 0 531 539 2064
Size: 531 8 1525
Fragments arranged by size:
1525 531 8
EarI CTCTTCn'nnn_
Cuts at: 0 284 344 1665 1717 2064
Size: 284 60 1321 52 347
Fragments arranged by size:
1321 347 284 60 52
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 155 251 301 1877 2064
Size: 155 96 50 1576 187
Fragments arranged by size:
1576 187 155 96 50
EcoO109I rG'GnC_Cy
Cuts at: 0 1624 1686 1901 1966 1967 1979 2064
Size: 1624 62 215 65 1 12 85
Fragments arranged by size:
1624 215 85 65 62 12 1
EcoRV GAT'ATC
Cuts at: 0 807 2064
Size: 807 1257
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 354 2064
Size: 354 1710
HaeII r_GCGC'y
Cuts at: 0 1390 2064
Size: 1390 674
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 55 87 549 581 945 977 1404 1436
Size: 55 32 462 32 364 32 427 32
Cuts at: 1436 1494 1526 1616 1648 2064
Size: 58 32 90 32 416
Fragments arranged by size:
462 427 416 364 90 58 55 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 1063 2016 2064
Size: 1063 953 48
Fragments arranged by size:
1063 953 48
MfeI C'AATT_G
Cuts at: 0 281 2064
Size: 281 1783
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 602 1688 2064
Size: 602 1086 376
Fragments arranged by size:
1086 602 376
MslI CAynn'nnrTG
Cuts at: 0 1471 2064
Size: 1471 593
MspA1I CmG'CkG
Cuts at: 0 682 691 1948 2064
Size: 682 9 1257 116
Fragments arranged by size:
1257 682 116 9
NcoI C'CATG_G
Cuts at: 0 1372 2064
Size: 1372 692
NspI r_CATG'y
Cuts at: 0 1522 2064
Size: 1522 542
PasI CC'CwG_GG
Cuts at: 0 766 2064
Size: 766 1298
PflMI CCAn_nnn'nTGG
Cuts at: 0 642 1273 1378 2064
Size: 642 631 105 686
Fragments arranged by size:
686 642 631 105
PfoI T'CCnGG_A
Cuts at: 0 1533 2064
Size: 1533 531
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 616 648 2064
Size: 616 32 1416
Fragments arranged by size:
1416 616 32
PpuMI rG'GwC_Cy
Cuts at: 0 1624 1686 1979 2064
Size: 1624 62 293 85
Fragments arranged by size:
1624 293 85 62
PspOMI G'GGCC_C
Cuts at: 0 1242 1966 2064
Size: 1242 724 98
Fragments arranged by size:
1242 724 98
PvuII CAG'CTG
Cuts at: 0 682 1948 2064
Size: 682 1266 116
Fragments arranged by size:
1266 682 116
SanDI GG'GwC_CC
Cuts at: 0 1624 2064
Size: 1624 440
SapI GCTCTTCn'nnn_
Cuts at: 0 344 2064
Size: 344 1720
ScaI AGT'ACT
Cuts at: 0 1402 2064
Size: 1402 662
SfcI C'TryA_G
Cuts at: 0 1763 2064
Size: 1763 301
SmlI C'TyrA_G
Cuts at: 0 597 1744 2064
Size: 597 1147 320
Fragments arranged by size:
1147 597 320
SspI AAT'ATT
Cuts at: 0 484 780 2064
Size: 484 296 1284
Fragments arranged by size:
1284 484 296
StuI AGG'CCT
Cuts at: 0 1825 2064
Size: 1825 239
StyI C'CwwG_G
Cuts at: 0 1372 2064
Size: 1372 692
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 178 1215 1395 2064
Size: 178 1037 180 669
Fragments arranged by size:
1037 669 180 178
TatI w'GTAC_w
Cuts at: 0 1400 1911 2064
Size: 1400 511 153
Fragments arranged by size:
1400 511 153
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 231 263 1620 1652 2064
Size: 231 32 1357 32 412
Fragments arranged by size:
1357 412 231 32 32
XmnI GAAnn'nnTTC
Cuts at: 0 631 2064
Size: 631 1433
Enzymes that do cut:
AcuI AfeI AflIII AhdI AleI AloI ApaI ApoI
AseI BamHI BanI BanII BbsI BglI BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaWI BsaXI
BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BsrDI
BsrGI BstEII BstYI Bsu36I BtgI BtsI EarI Eco57MI
EcoO109I EcoRV FalI HaeII Hin4I HincII MfeI MmeI
MslI MspA1I NcoI NspI PasI PflMI PfoI PpiI
PpuMI PspOMI PvuII SanDI SapI ScaI SfcI SmlI
SspI StuI StyI TaqII TatI TstI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AgeI AlfI
AlwNI ApaLI AscI AsiSI AvaI AvrII BaeI BbeI
BbvCI BcgI BciVI BclI BfrBI BglII BmtI BsaI
BsaAI BsaBI BsaHI BsiEI BsiWI BspEI BspHI BspMI
BsrBI BsrFI BssHII BssSI BstAPI BstBI BstXI BstZ17I
BtgZI ClaI CspCI DraI DraIII DrdI EaeI EagI
EciI EcoICRI EcoNI EcoRI FseI FspI FspAI HindIII
HpaI KasI KpnI MluI MscI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI PacI PciI PmeI
PmlI PshAI PsiI PspXI PsrI PstI PvuI RsrII
SacI SacII SalI SbfI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SwaI Tth111I
XbaI XcmI XhoI XmaI ZraI