Restriction map for
pFN21AE2045
(Linear) (Six-base) MAPSORT of: KIEE2045.seq Check: 4600 from: 1 to: 1989
KIEE2045.orf
With 182 enzymes: SgfI *
July 15, 2010 18:53 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 553 763 787 928 1975 1989
Size: 553 210 24 141 1047 14
Fragments arranged by size:
1047 553 210 141 24 14
AflIII A'CryG_T
Cuts at: 0 221 365 564 1989
Size: 221 144 199 1425
Fragments arranged by size:
1425 221 199 144
AleI CACnn'nnGTG
Cuts at: 0 1510 1989
Size: 1510 479
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 262 1989
Size: 262 1727
ApaI G_GGCC'C
Cuts at: 0 256 1209 1989
Size: 256 953 780
Fragments arranged by size:
953 780 256
ApaLI G'TGCA_C
Cuts at: 0 1605 1989
Size: 1605 384
ApoI r'AATT_y
Cuts at: 0 848 1044 1235 1989
Size: 848 196 191 754
Fragments arranged by size:
848 754 196 191
AvaI C'yCGr_G
Cuts at: 0 325 762 1202 1989
Size: 325 437 440 787
Fragments arranged by size:
787 440 437 325
BanI G'GyrC_C
Cuts at: 0 331 703 1130 1812 1823 1989
Size: 331 372 427 682 11 166
Fragments arranged by size:
682 427 372 331 166 11
BanII G_rGCy'C
Cuts at: 0 176 256 546 586 920 1209 1989
Size: 176 80 290 40 334 289 780
Fragments arranged by size:
780 334 290 289 176 80 40
BbsI GAAGACnn'nnnn_
Cuts at: 0 608 1590 1756 1989
Size: 608 982 166 233
Fragments arranged by size:
982 608 233 166
BbvCI CC'TCA_GC
Cuts at: 0 1103 1989
Size: 1103 886
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1774 1808 1989
Size: 1774 34 181
Fragments arranged by size:
1774 181 34
BclI T'GATC_A
Cuts at: 0 147 609 1989
Size: 147 462 1380
Fragments arranged by size:
1380 462 147
BglI GCCn_nnn'nGGC
Cuts at: 0 31 1821 1989
Size: 31 1790 168
Fragments arranged by size:
1790 168 31
BglII A'GATC_T
Cuts at: 0 410 614 1989
Size: 410 204 1375
Fragments arranged by size:
1375 410 204
BlpI GC'TnA_GC
Cuts at: 0 1301 1989
Size: 1301 688
Bme1580I G_kGCm'C
Cuts at: 0 256 334 639 1209 1609 1826 1989
Size: 256 78 305 570 400 217 163
Fragments arranged by size:
570 400 305 256 217 163 78
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 124 278 568 729 811 1989
Size: 124 154 290 161 82 1178
Fragments arranged by size:
1178 290 161 154 124 82
Bpu10I CC'TnA_GC
Cuts at: 0 316 580 1103 1478 1989
Size: 316 264 523 375 511
Fragments arranged by size:
523 511 375 316 264
BsaAI yAC'GTr
Cuts at: 0 1604 1989
Size: 1604 385
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 127 157 1268 1298 1989
Size: 127 30 1111 30 691
Fragments arranged by size:
1111 691 127 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 148 151 154 517 884 1989
Size: 148 3 3 363 367 1105
Fragments arranged by size:
1105 367 363 148 3 3
BseYI C'CCAG_C
Cuts at: 0 293 419 967 1356 1613 1960 1989
Size: 293 126 548 389 257 347 29
Fragments arranged by size:
548 389 347 293 257 126 29
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 85 268 319 1045 1129 1989
Size: 85 183 51 726 84 860
Fragments arranged by size:
860 726 183 85 84 51
BsiEI CG_ry'CG
Cuts at: 0 121 237 1989
Size: 121 116 1752
Fragments arranged by size:
1752 121 116
BsiHKAI G_wGCw'C
Cuts at: 0 586 1258 1374 1609 1989
Size: 586 672 116 235 380
Fragments arranged by size:
672 586 380 235 116
BsmBI CGTCTCn'nnnn_
Cuts at: 0 717 1989
Size: 717 1272
Bsp1286I G_dGCh'C
Cuts at: 0 176 256 334 546 586 639 920 1209
Size: 176 80 78 212 40 53 281 289
Cuts at: 1209 1258 1374 1609 1826 1989
Size: 49 116 235 217 163
Fragments arranged by size:
289 281 235 217 212 176 163 116
80 78 53 49 40
BspHI T'CATG_A
Cuts at: 0 150 1989
Size: 150 1839
BsrDI GCAATG_nn'
Cuts at: 0 1409 1989
Size: 1409 580
BsrFI r'CCGG_y
Cuts at: 0 120 1989
Size: 120 1869
BsrGI T'GTAC_A
Cuts at: 0 1275 1989
Size: 1275 714
BssSI C'ACGA_G
Cuts at: 0 1063 1989
Size: 1063 926
BstEII G'GTnAC_C
Cuts at: 0 440 1989
Size: 440 1549
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1016 1422 1731 1989
Size: 1016 406 309 258
Fragments arranged by size:
1016 406 309 258
BstYI r'GATC_y
Cuts at: 0 410 614 1067 1989
Size: 410 204 453 922
Fragments arranged by size:
922 453 410 204
BtgI C'CryG_G
Cuts at: 0 938 1187 1989
Size: 938 249 802
Fragments arranged by size:
938 802 249
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 438 1095 1989
Size: 438 657 894
Fragments arranged by size:
894 657 438
BtsI GCAGTG_nn'
Cuts at: 0 1256 1607 1989
Size: 1256 351 382
Fragments arranged by size:
1256 382 351
DraIII CAC_nnn'GTG
Cuts at: 0 1098 1592 1989
Size: 1098 494 397
Fragments arranged by size:
1098 494 397
EaeI y'GGCC_r
Cuts at: 0 23 118 1721 1772 1989
Size: 23 95 1603 51 217
Fragments arranged by size:
1603 217 95 51 23
EagI C'GGCC_G
Cuts at: 0 118 1989
Size: 118 1871
EarI CTCTTCn'nnn_
Cuts at: 0 46 502 625 767 1447 1989
Size: 46 456 123 142 680 542
Fragments arranged by size:
680 542 456 142 123 46
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 112 1989
Size: 112 1877
EcoICRI GAG'CTC
Cuts at: 0 584 1989
Size: 584 1405
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 124 278 553 568 729 763 787 811
Size: 124 154 275 15 161 34 24 24
Cuts at: 811 928 1975 1989
Size: 117 1047 14
Fragments arranged by size:
1047 275 161 154 124 117 34 24
24 15 14
EcoNI CCTnn'n_nnAGG
Cuts at: 0 325 1989
Size: 325 1664
EcoO109I rG'GnC_Cy
Cuts at: 0 32 252 253 1350 1473 1659 1849 1989
Size: 32 220 1 1097 123 186 190 140
Fragments arranged by size:
1097 220 190 186 140 123 32 1
EcoRI G'AATT_C
Cuts at: 0 848 1989
Size: 848 1141
FspI TGC'GCA
Cuts at: 0 196 1989
Size: 196 1793
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 395 427 942 974 1059 1091 1989
Size: 395 32 515 32 85 32 898
Fragments arranged by size:
898 515 395 85 32 32 32
HincII GTy'rAC
Cuts at: 0 1570 1989
Size: 1570 419
MluI A'CGCG_T
Cuts at: 0 221 1989
Size: 221 1768
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1000 1080 1989
Size: 1000 80 909
Fragments arranged by size:
1000 909 80
MscI TGG'CCA
Cuts at: 0 25 1723 1774 1989
Size: 25 1698 51 215
Fragments arranged by size:
1698 215 51 25
MslI CAynn'nnrTG
Cuts at: 0 19 244 1510 1729 1989
Size: 19 225 1266 219 260
Fragments arranged by size:
1266 260 225 219 19
MspA1I CmG'CkG
Cuts at: 0 114 293 1305 1641 1964 1989
Size: 114 179 1012 336 323 25
Fragments arranged by size:
1012 336 323 179 114 25
NaeI GCC'GGC
Cuts at: 0 122 1989
Size: 122 1867
NcoI C'CATG_G
Cuts at: 0 938 1187 1989
Size: 938 249 802
Fragments arranged by size:
938 802 249
NgoMIV G'CCGG_C
Cuts at: 0 120 1989
Size: 120 1869
NspI r_CATG'y
Cuts at: 0 369 1621 1989
Size: 369 1252 368
Fragments arranged by size:
1252 369 368
PciI A'CATG_T
Cuts at: 0 365 1989
Size: 365 1624
PflMI CCAn_nnn'nTGG
Cuts at: 0 210 1781 1989
Size: 210 1571 208
Fragments arranged by size:
1571 210 208
PfoI T'CCnGG_A
Cuts at: 0 586 1989
Size: 586 1403
PmlI CAC'GTG
Cuts at: 0 1604 1989
Size: 1604 385
PpuMI rG'GwC_Cy
Cuts at: 0 1473 1659 1989
Size: 1473 186 330
Fragments arranged by size:
1473 330 186
PspOMI G'GGCC_C
Cuts at: 0 252 1205 1989
Size: 252 953 784
Fragments arranged by size:
953 784 252
PstI C_TGCA'G
Cuts at: 0 113 182 462 935 1487 1989
Size: 113 69 280 473 552 502
Fragments arranged by size:
552 502 473 280 113 69
PvuII CAG'CTG
Cuts at: 0 114 1305 1641 1964 1989
Size: 114 1191 336 323 25
Fragments arranged by size:
1191 336 323 114 25
SacI G_AGCT'C
Cuts at: 0 586 1989
Size: 586 1403
SanDI GG'GwC_CC
Cuts at: 0 1473 1659 1989
Size: 1473 186 330
Fragments arranged by size:
1473 330 186
SapI GCTCTTCn'nnn_
Cuts at: 0 46 767 1989
Size: 46 721 1222
Fragments arranged by size:
1222 721 46
SexAI A'CCwGG_T
Cuts at: 0 1094 1989
Size: 1094 895
SfcI C'TryA_G
Cuts at: 0 109 178 458 931 1483 1989
Size: 109 69 280 473 552 506
Fragments arranged by size:
552 506 473 280 109 69
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 31 1989
Size: 31 1958
SmaI CCC'GGG
Cuts at: 0 1204 1989
Size: 1204 785
SphI G_CATG'C
Cuts at: 0 1621 1989
Size: 1621 368
SspI AAT'ATT
Cuts at: 0 1840 1989
Size: 1840 149
StuI AGG'CCT
Cuts at: 0 1489 1759 1989
Size: 1489 270 230
Fragments arranged by size:
1489 270 230
StyI C'CwwG_G
Cuts at: 0 938 1187 1703 1989
Size: 938 249 516 286
Fragments arranged by size:
938 516 286 249
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 285 1550 1710 1989
Size: 285 1265 160 279
Fragments arranged by size:
1265 285 279 160
TatI w'GTAC_w
Cuts at: 0 1275 1989
Size: 1275 714
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 208 240 1989
Size: 208 32 1749
Fragments arranged by size:
1749 208 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 697 1989
Size: 697 1292
XmaI C'CCGG_G
Cuts at: 0 1202 1989
Size: 1202 787
XmnI GAAnn'nnTTC
Cuts at: 0 776 1989
Size: 776 1213
Enzymes that do cut:
AcuI AflIII AleI AlfI ApaI ApaLI ApoI AvaI
BanI BanII BbsI BbvCI BcgI BclI BglI BglII
BlpI Bme1580I BpmI Bpu10I BsaAI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspHI BsrDI BsrFI
BsrGI BssSI BstEII BstXI BstYI BtgI BtgZI BtsI
DraIII EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FspI Hin4I HincII MluI MmeI MscI
MslI MspA1I NaeI NcoI NgoMIV NspI PciI PflMI
PfoI PmlI PpuMI PspOMI PstI PvuII SacI SanDI
SapI SexAI SfcI SfiI SmaI SphI SspI StuI
StyI TaqII TatI TstI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AloI AlwNI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BciVI BfrBI BmgBI BmrI BmtI BplI
BpuEI BsaI BsaBI BsaHI BsaWI BsiWI BsmI BspEI
BspMI BsrBI BssHII BstAPI BstBI BstZ17I Bsu36I ClaI
CspCI DraI DrdI EcoRV FalI FseI FspAI HaeII
HindIII HpaI KasI KpnI MfeI NarI NdeI NheI
NotI NruI NsiI PacI PasI PmeI PpiI PshAI
PsiI PspXI PsrI PvuI RsrII SacII SalI SbfI
ScaI SfoI SgfI SgrAI SmlI SnaBI SpeI SrfI
SwaI Tth111I XbaI XhoI ZraI