Restriction map for
pFN21AE2041
(Linear) (Six-base) MAPSORT of: KIEE2041.seq Check: 4498 from: 1 to: 1959
KIEE2041.orf
With 182 enzymes: SgfI *
July 15, 2010 18:53 ..
AccI GT'mk_AC
Cuts at: 0 665 1959
Size: 665 1294
Acc65I G'GTAC_C
Cuts at: 0 77 1959
Size: 77 1882
AclI AA'CG_TT
Cuts at: 0 1610 1959
Size: 1610 349
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 121 469 1959
Size: 121 348 1490
Fragments arranged by size:
1490 348 121
AflIII A'CryG_T
Cuts at: 0 1619 1959
Size: 1619 340
ApoI r'AATT_y
Cuts at: 0 462 621 743 796 882 1768 1959
Size: 462 159 122 53 86 886 191
Fragments arranged by size:
886 462 191 159 122 86 53
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 69 102 812 845 1959
Size: 69 33 710 33 1114
Fragments arranged by size:
1114 710 69 33 33
BanI G'GyrC_C
Cuts at: 0 77 1959
Size: 77 1882
BbsI GAAGACnn'nnnn_
Cuts at: 0 1931 1959
Size: 1931 28
BciVI GTATCCnnnnn_n'
Cuts at: 0 1392 1959
Size: 1392 567
BclI T'GATC_A
Cuts at: 0 62 1740 1959
Size: 62 1678 219
Fragments arranged by size:
1678 219 62
BglII A'GATC_T
Cuts at: 0 34 1702 1959
Size: 34 1668 257
Fragments arranged by size:
1668 257 34
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 394 1959
Size: 394 1565
BsaI GGTCTCn'nnnn_
Cuts at: 0 568 862 944 1208 1959
Size: 568 294 82 264 751
Fragments arranged by size:
751 568 294 264 82
BsaAI yAC'GTr
Cuts at: 0 1121 1959
Size: 1121 838
BsaBI GATnn'nnATC
Cuts at: 0 1195 1701 1959
Size: 1195 506 258
Fragments arranged by size:
1195 506 258
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1142 1316 1325 1959
Size: 1142 174 9 634
Fragments arranged by size:
1142 634 174 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 664 1959
Size: 664 1295
BspHI T'CATG_A
Cuts at: 0 1206 1239 1959
Size: 1206 33 720
Fragments arranged by size:
1206 720 33
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 686 1959
Size: 686 1273
BsrGI T'GTAC_A
Cuts at: 0 1656 1691 1959
Size: 1656 35 268
Fragments arranged by size:
1656 268 35
BssSI C'ACGA_G
Cuts at: 0 1466 1959
Size: 1466 493
BstBI TT'CG_AA
Cuts at: 0 1579 1959
Size: 1579 380
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1851 1959
Size: 1851 108
BstYI r'GATC_y
Cuts at: 0 34 632 1024 1567 1702 1959
Size: 34 598 392 543 135 257
Fragments arranged by size:
598 543 392 257 135 34
BtgI C'CryG_G
Cuts at: 0 852 1561 1760 1959
Size: 852 709 199 199
Fragments arranged by size:
852 709 199 199
DraI TTT'AAA
Cuts at: 0 1895 1959
Size: 1895 64
EarI CTCTTCn'nnn_
Cuts at: 0 221 1388 1959
Size: 221 1167 571
Fragments arranged by size:
1167 571 221
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 121 469 1959
Size: 121 348 1490
Fragments arranged by size:
1490 348 121
EcoNI CCTnn'n_nnAGG
Cuts at: 0 573 1959
Size: 573 1386
EcoRI G'AATT_C
Cuts at: 0 621 796 1959
Size: 621 175 1163
Fragments arranged by size:
1163 621 175
EcoRV GAT'ATC
Cuts at: 0 863 1959
Size: 863 1096
HaeII r_GCGC'y
Cuts at: 0 1418 1959
Size: 1418 541
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 949 981 1295 1327 1959
Size: 949 32 314 32 632
Fragments arranged by size:
949 632 314 32 32
HincII GTy'rAC
Cuts at: 0 831 1959
Size: 831 1128
HindIII A'AGCT_T
Cuts at: 0 1837 1959
Size: 1837 122
KpnI G_GTAC'C
Cuts at: 0 81 1959
Size: 81 1878
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 179 1500 1959
Size: 179 1321 459
Fragments arranged by size:
1321 459 179
MslI CAynn'nnrTG
Cuts at: 0 1805 1817 1919 1959
Size: 1805 12 102 40
Fragments arranged by size:
1805 102 40 12
NcoI C'CATG_G
Cuts at: 0 852 1561 1959
Size: 852 709 398
Fragments arranged by size:
852 709 398
NspI r_CATG'y
Cuts at: 0 1623 1959
Size: 1623 336
PciI A'CATG_T
Cuts at: 0 1619 1959
Size: 1619 340
PflMI CCAn_nnn'nTGG
Cuts at: 0 1395 1959
Size: 1395 564
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 380 412 1959
Size: 380 32 1547
Fragments arranged by size:
1547 380 32
SapI GCTCTTCn'nnn_
Cuts at: 0 221 1959
Size: 221 1738
SmlI C'TyrA_G
Cuts at: 0 373 1959
Size: 373 1586
StuI AGG'CCT
Cuts at: 0 379 1751 1959
Size: 379 1372 208
Fragments arranged by size:
1372 379 208
StyI C'CwwG_G
Cuts at: 0 852 1561 1953 1959
Size: 852 709 392 6
Fragments arranged by size:
852 709 392 6
TatI w'GTAC_w
Cuts at: 0 1656 1691 1959
Size: 1656 35 268
Fragments arranged by size:
1656 268 35
XmnI GAAnn'nnTTC
Cuts at: 0 621 1506 1769 1959
Size: 621 885 263 190
Fragments arranged by size:
885 621 263 190
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII ApoI BaeI BanI
BbsI BciVI BclI BglII BpuEI BsaI BsaAI BsaBI
BseRI BsgI BspHI BspMI BsrGI BssSI BstBI BstXI
BstYI BtgI DraI EarI Eco57MI EcoNI EcoRI EcoRV
HaeII Hin4I HincII HindIII KpnI MmeI MslI NcoI
NspI PciI PflMI PsrI SapI SmlI StuI StyI
TatI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AlfI
AloI AlwNI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BamHI BanII BbeI BbvCI BcgI BfrBI BglI
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BsaHI BsaWI BsaXI BseYI BsiEI BsiHKAI BsiWI BsmI
BsmBI Bsp1286I BspEI BsrBI BsrDI BsrFI BssHII BstAPI
BstEII BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraIII
DrdI EaeI EagI EciI EcoICRI EcoO109I FalI FseI
FspI FspAI HpaI KasI MfeI MluI MscI MspA1I
NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI SwaI TaqII TstI Tth111I XbaI XcmI XhoI
XmaI ZraI