Restriction map for
pFN21AE2040
(Linear) (Six-base) MAPSORT of: KIEE2040.seq Check: 5044 from: 1 to: 1953
KIEE2040.orf
With 182 enzymes: SgfI *
July 15, 2010 18:53 ..
AccI GT'mk_AC
Cuts at: 0 1541 1747 1953
Size: 1541 206 206
Fragments arranged by size:
1541 206 206
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 865 1953
Size: 865 1088
AfeI AGC'GCT
Cuts at: 0 487 1953
Size: 487 1466
AflIII A'CryG_T
Cuts at: 0 1048 1953
Size: 1048 905
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 347 379 1953
Size: 347 32 1574
Fragments arranged by size:
1574 347 32
ApaI G_GGCC'C
Cuts at: 0 1044 1953
Size: 1044 909
AseI AT'TA_AT
Cuts at: 0 749 1556 1953
Size: 749 807 397
Fragments arranged by size:
807 749 397
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 767 800 1953
Size: 767 33 1153
Fragments arranged by size:
1153 767 33
BamHI G'GATC_C
Cuts at: 0 954 1953
Size: 954 999
BanI G'GyrC_C
Cuts at: 0 1239 1953
Size: 1239 714
BanII G_rGCy'C
Cuts at: 0 1044 1312 1953
Size: 1044 268 641
Fragments arranged by size:
1044 641 268
BbsI GAAGACnn'nnnn_
Cuts at: 0 1483 1953
Size: 1483 470
BciVI GTATCCnnnnn_n'
Cuts at: 0 1524 1953
Size: 1524 429
BclI T'GATC_A
Cuts at: 0 1710 1770 1953
Size: 1710 60 183
Fragments arranged by size:
1710 183 60
Bme1580I G_kGCm'C
Cuts at: 0 1044 1242 1953
Size: 1044 198 711
Fragments arranged by size:
1044 711 198
BmrI ACTGGGnnnn_n'
Cuts at: 0 385 1953
Size: 385 1568
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1493 1953
Size: 1493 460
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1326 1953
Size: 1326 627
BsaHI Gr'CG_yC
Cuts at: 0 211 1953
Size: 211 1742
BsaWI w'CCGG_w
Cuts at: 0 399 912 1953
Size: 399 513 1041
Fragments arranged by size:
1041 513 399
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 252 282 347 377 1657 1687 1953
Size: 252 30 65 30 1280 30 266
Fragments arranged by size:
1280 266 252 65 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1743 1953
Size: 1743 210
BseYI C'CCAG_C
Cuts at: 0 736 1953
Size: 736 1217
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 553 1802 1953
Size: 553 1249 151
Fragments arranged by size:
1249 553 151
BsiHKAI G_wGCw'C
Cuts at: 0 1312 1953
Size: 1312 641
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1340 1736 1953
Size: 1340 396 217
Fragments arranged by size:
1340 396 217
Bsp1286I G_dGCh'C
Cuts at: 0 1044 1242 1312 1953
Size: 1044 198 70 641
Fragments arranged by size:
1044 641 198 70
BspEI T'CCGG_A
Cuts at: 0 399 912 1953
Size: 399 513 1041
Fragments arranged by size:
1041 513 399
BspHI T'CATG_A
Cuts at: 0 206 1953
Size: 206 1747
BsrGI T'GTAC_A
Cuts at: 0 1195 1953
Size: 1195 758
BstEII G'GTnAC_C
Cuts at: 0 301 694 1953
Size: 301 393 1259
Fragments arranged by size:
1259 393 301
BstYI r'GATC_y
Cuts at: 0 954 1953
Size: 954 999
BstZ17I GTA'TAC
Cuts at: 0 1748 1953
Size: 1748 205
Bsu36I CC'TnA_GG
Cuts at: 0 985 1861 1953
Size: 985 876 92
Fragments arranged by size:
985 876 92
BtgI C'CryG_G
Cuts at: 0 551 836 1953
Size: 551 285 1117
Fragments arranged by size:
1117 551 285
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 299 1953
Size: 299 1654
EarI CTCTTCn'nnn_
Cuts at: 0 341 389 1403 1412 1953
Size: 341 48 1014 9 541
Fragments arranged by size:
1014 541 341 48 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 19 1953
Size: 19 1934
EcoICRI GAG'CTC
Cuts at: 0 1310 1953
Size: 1310 643
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 865 1493 1953
Size: 865 628 460
Fragments arranged by size:
865 628 460
EcoO109I rG'GnC_Cy
Cuts at: 0 343 1041 1436 1953
Size: 343 698 395 517
Fragments arranged by size:
698 517 395 343
HaeII r_GCGC'y
Cuts at: 0 489 1953
Size: 489 1464
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 939 971 1384 1416 1425 1457 1897 1929
Size: 939 32 413 32 9 32 440 32
Cuts at: 1929 1953
Size: 24
Fragments arranged by size:
939 440 413 32 32 32 32 24
9
HincII GTy'rAC
Cuts at: 0 947 1953
Size: 947 1006
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 792 1877 1953
Size: 792 1085 76
Fragments arranged by size:
1085 792 76
MslI CAynn'nnrTG
Cuts at: 0 1090 1953
Size: 1090 863
MspA1I CmG'CkG
Cuts at: 0 1687 1953
Size: 1687 266
NcoI C'CATG_G
Cuts at: 0 551 836 1953
Size: 551 285 1117
Fragments arranged by size:
1117 551 285
NdeI CA'TA_TG
Cuts at: 0 1146 1953
Size: 1146 807
NspI r_CATG'y
Cuts at: 0 1052 1953
Size: 1052 901
PacI TTA_AT'TAA
Cuts at: 0 935 1953
Size: 935 1018
PciI A'CATG_T
Cuts at: 0 1048 1953
Size: 1048 905
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 347 379 1953
Size: 347 32 1574
Fragments arranged by size:
1574 347 32
PpuMI rG'GwC_Cy
Cuts at: 0 343 1436 1953
Size: 343 1093 517
Fragments arranged by size:
1093 517 343
PshAI GACnn'nnGTC
Cuts at: 0 479 1953
Size: 479 1474
PspOMI G'GGCC_C
Cuts at: 0 1040 1953
Size: 1040 913
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 44 76 1953
Size: 44 32 1877
Fragments arranged by size:
1877 44 32
PstI C_TGCA'G
Cuts at: 0 219 816 1692 1953
Size: 219 597 876 261
Fragments arranged by size:
876 597 261 219
SacI G_AGCT'C
Cuts at: 0 1312 1953
Size: 1312 641
SapI GCTCTTCn'nnn_
Cuts at: 0 389 1412 1953
Size: 389 1023 541
Fragments arranged by size:
1023 541 389
ScaI AGT'ACT
Cuts at: 0 62 1192 1794 1953
Size: 62 1130 602 159
Fragments arranged by size:
1130 602 159 62
SfcI C'TryA_G
Cuts at: 0 144 180 215 812 1538 1578 1688 1953
Size: 144 36 35 597 726 40 110 265
Fragments arranged by size:
726 597 265 144 110 40 36 35
SmlI C'TyrA_G
Cuts at: 0 1305 1953
Size: 1305 648
StyI C'CwwG_G
Cuts at: 0 551 836 1953
Size: 551 285 1117
Fragments arranged by size:
1117 551 285
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 404 663 1617 1953
Size: 404 259 954 336
Fragments arranged by size:
954 404 336 259
TatI w'GTAC_w
Cuts at: 0 60 585 1190 1195 1263 1792 1953
Size: 60 525 605 5 68 529 161
Fragments arranged by size:
605 529 525 161 68 60 5
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 558 590 1330 1362 1953
Size: 558 32 740 32 591
Fragments arranged by size:
740 591 558 32 32
XbaI T'CTAG_A
Cuts at: 0 922 1953
Size: 922 1031
XmnI GAAnn'nnTTC
Cuts at: 0 831 1953
Size: 831 1122
Enzymes that do cut:
AccI AcuI AfeI AflIII AloI ApaI AseI BaeI
BamHI BanI BanII BbsI BciVI BclI Bme1580I BmrI
BpmI BpuEI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BsrGI BstEII BstYI
BstZ17I Bsu36I BtgI BtgZI EarI EciI EcoICRI Eco57MI
EcoO109I HaeII Hin4I HincII MmeI MslI MspA1I NcoI
NdeI NspI PacI PciI PpiI PpuMI PshAI PspOMI
PsrI PstI SacI SapI ScaI SfcI SmlI StyI
TaqII TatI TstI XbaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AgeI AhdI AleI
AlfI AlwNI ApaLI ApoI AscI AsiSI AvaI AvrII
BbeI BbvCI BcgI BfrBI BglI BglII BlpI BmgBI
BmtI BplI Bpu10I BsaI BsaAI BsaBI BsiEI BsiWI
BsmI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI
BstBI BstXI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EcoNI EcoRI EcoRV FalI FseI FspI
FspAI HindIII HpaI KasI KpnI MfeI MluI MscI
NaeI NarI NgoMIV NheI NotI NruI NsiI PasI
PflMI PfoI PmeI PmlI PsiI PspXI PvuI PvuII
RsrII SacII SalI SanDI SbfI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
StuI SwaI Tth111I XcmI XhoI XmaI ZraI