Restriction map for
pFN21AE2039
(Linear) (Six-base) MAPSORT of: KIEE2039.seq Check: 583 from: 1 to: 1944
KIEE2039.orf
With 182 enzymes: SgfI *
July 15, 2010 18:53 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 608 1586 1944
Size: 608 978 358
Fragments arranged by size:
978 608 358
AatII G_ACGT'C
Cuts at: 0 131 1944
Size: 131 1813
Acc65I G'GTAC_C
Cuts at: 0 974 1944
Size: 974 970
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 654 896 925 1944
Size: 654 242 29 1019
Fragments arranged by size:
1019 654 242 29
AfeI AGC'GCT
Cuts at: 0 60 1944
Size: 60 1884
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1069 1944
Size: 1069 875
ApaI G_GGCC'C
Cuts at: 0 835 874 1851 1944
Size: 835 39 977 93
Fragments arranged by size:
977 835 93 39
ApoI r'AATT_y
Cuts at: 0 1507 1944
Size: 1507 437
AvaI C'yCGr_G
Cuts at: 0 683 769 921 1174 1182 1387 1744 1844
Size: 683 86 152 253 8 205 357 100
Cuts at: 1844 1915 1944
Size: 71 29
Fragments arranged by size:
683 357 253 205 152 100 86 71
29 8
AvrII C'CTAG_G
Cuts at: 0 969 1944
Size: 969 975
BamHI G'GATC_C
Cuts at: 0 651 1944
Size: 651 1293
BanI G'GyrC_C
Cuts at: 0 478 706 974 1933 1944
Size: 478 228 268 959 11
Fragments arranged by size:
959 478 268 228 11
BanII G_rGCy'C
Cuts at: 0 147 311 329 346 770 835 874 937
Size: 147 164 18 17 424 65 39 63
Cuts at: 937 1075 1340 1559 1799 1851 1944
Size: 138 265 219 240 52 93
Fragments arranged by size:
424 265 240 219 164 147 138 93
65 63 52 39 18 17
BbeI G_GCGC'C
Cuts at: 0 1937 1944
Size: 1937 7
BbsI GAAGACnn'nnnn_
Cuts at: 0 549 1944
Size: 549 1395
BbvCI CC'TCA_GC
Cuts at: 0 406 502 979 998 1944
Size: 406 96 477 19 946
Fragments arranged by size:
946 477 406 96 19
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 556 590 1104 1138 1288 1322 1944
Size: 556 34 514 34 150 34 622
Fragments arranged by size:
622 556 514 150 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1102 1944
Size: 1102 842
BglI GCCn_nnn'nGGC
Cuts at: 0 341 869 1944
Size: 341 528 1075
Fragments arranged by size:
1075 528 341
BlpI GC'TnA_GC
Cuts at: 0 1684 1944
Size: 1684 260
Bme1580I G_kGCm'C
Cuts at: 0 481 711 835 861 874 1851 1944
Size: 481 230 124 26 13 977 93
Fragments arranged by size:
977 481 230 124 93 26 13
BmgBI CAC'GTC
Cuts at: 0 1698 1944
Size: 1698 246
BmrI ACTGGGnnnn_n'
Cuts at: 0 150 273 1944
Size: 150 123 1671
Fragments arranged by size:
1671 150 123
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 103 1944
Size: 103 1841
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 182 183 461 631 821 1924 1944
Size: 182 1 278 170 190 1103 20
Fragments arranged by size:
1103 278 190 182 170 20 1
Bpu10I CC'TnA_GC
Cuts at: 0 406 502 899 979 998 1076 1944
Size: 406 96 397 80 19 78 868
Fragments arranged by size:
868 406 397 96 80 78 19
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1639 1944
Size: 1639 305
BsaI GGTCTCn'nnnn_
Cuts at: 0 1363 1944
Size: 1363 581
BsaAI yAC'GTr
Cuts at: 0 867 1944
Size: 867 1077
BsaHI Gr'CG_yC
Cuts at: 0 128 1934 1944
Size: 128 1806 10
Fragments arranged by size:
1806 128 10
BsaWI w'CCGG_w
Cuts at: 0 911 1478 1944
Size: 911 567 466
Fragments arranged by size:
911 567 466
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 364 394 629 659 1531 1561 1944
Size: 364 30 235 30 872 30 383
Fragments arranged by size:
872 383 364 235 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 169 241 561 862 865 1015 1201 1933
Size: 169 72 320 301 3 150 186 732
Cuts at: 1933 1936 1944
Size: 3 8
Fragments arranged by size:
732 320 301 186 169 150 72 8
3 3
BseYI C'CCAG_C
Cuts at: 0 328 410 804 937 983 1188 1944
Size: 328 82 394 133 46 205 756
Fragments arranged by size:
756 394 328 205 133 82 46
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 34 121 341 445 1558 1705 1783 1944
Size: 34 87 220 104 1113 147 78 161
Fragments arranged by size:
1113 220 161 147 104 87 78 34
BsiHKAI G_wGCw'C
Cuts at: 0 1007 1340 1944
Size: 1007 333 604
Fragments arranged by size:
1007 604 333
Bsp1286I G_dGCh'C
Cuts at: 0 147 311 329 346 481 711 770 835
Size: 147 164 18 17 135 230 59 65
Cuts at: 835 861 874 937 1007 1075 1340 1559 1799
Size: 26 13 63 70 68 265 219 240
Cuts at: 1799 1851 1944
Size: 52 93
Fragments arranged by size:
265 240 230 219 164 147 135 93
70 68 65 63 59 52 26 18
17 13
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 56 521 608 882 1586 1944
Size: 56 465 87 274 704 358
Fragments arranged by size:
704 465 358 274 87 56
BsrBI CCG'CTC
Cuts at: 0 392 828 1086 1944
Size: 392 436 258 858
Fragments arranged by size:
858 436 392 258
BsrFI r'CCGG_y
Cuts at: 0 10 1944
Size: 10 1934
BssSI C'ACGA_G
Cuts at: 0 312 1339 1944
Size: 312 1027 605
Fragments arranged by size:
1027 605 312
BstEII G'GTnAC_C
Cuts at: 0 429 1944
Size: 429 1515
BstYI r'GATC_y
Cuts at: 0 651 914 1649 1769 1907 1944
Size: 651 263 735 120 138 37
Fragments arranged by size:
735 651 263 138 120 37
Bsu36I CC'TnA_GG
Cuts at: 0 725 1408 1944
Size: 725 683 536
Fragments arranged by size:
725 683 536
BtgI C'CryG_G
Cuts at: 0 471 821 1871 1944
Size: 471 350 1050 73
Fragments arranged by size:
1050 471 350 73
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 75 1944
Size: 75 1869
BtsI GCAGTG_nn'
Cuts at: 0 457 1063 1944
Size: 457 606 881
Fragments arranged by size:
881 606 457
DraIII CAC_nnn'GTG
Cuts at: 0 1761 1944
Size: 1761 183
DrdI GACnn_nn'nnGTC
Cuts at: 0 127 268 1944
Size: 127 141 1676
Fragments arranged by size:
1676 141 127
EaeI y'GGCC_r
Cuts at: 0 286 521 814 1944
Size: 286 235 293 1130
Fragments arranged by size:
1130 293 286 235
EarI CTCTTCn'nnn_
Cuts at: 0 187 1352 1944
Size: 187 1165 592
Fragments arranged by size:
1165 592 187
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 53 376 1944
Size: 53 323 1568
Fragments arranged by size:
1568 323 53
EcoICRI GAG'CTC
Cuts at: 0 1338 1944
Size: 1338 606
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 182 183 461 631 654 821 896 925
Size: 182 1 278 170 23 167 75 29
Cuts at: 925 1924 1944
Size: 999 20
Fragments arranged by size:
999 278 182 170 167 75 29 23
20 1
EcoNI CCTnn'n_nnAGG
Cuts at: 0 889 1915 1944
Size: 889 1026 29
Fragments arranged by size:
1026 889 29
EcoO109I rG'GnC_Cy
Cuts at: 0 808 831 832 871 894 1171 1716 1848
Size: 808 23 1 39 23 277 545 132
Cuts at: 1848 1944
Size: 96
Fragments arranged by size:
808 545 277 132 96 39 23 23
1
EcoRI G'AATT_C
Cuts at: 0 1507 1944
Size: 1507 437
EcoRV GAT'ATC
Cuts at: 0 1206 1944
Size: 1206 738
HaeII r_GCGC'y
Cuts at: 0 62 1937 1944
Size: 62 1875 7
Fragments arranged by size:
1875 62 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 67 99 364 396 643 675 770 802
Size: 67 32 265 32 247 32 95 32
Cuts at: 802 1255 1287 1444 1476 1944
Size: 453 32 157 32 468
Fragments arranged by size:
468 453 265 247 157 95 67 32
32 32 32 32 32
KasI G'GCGC_C
Cuts at: 0 1933 1944
Size: 1933 11
KpnI G_GTAC'C
Cuts at: 0 978 1944
Size: 978 966
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 608 764 1944
Size: 608 156 1180
Fragments arranged by size:
1180 608 156
MscI TGG'CCA
Cuts at: 0 523 1944
Size: 523 1421
MslI CAynn'nnrTG
Cuts at: 0 1621 1944
Size: 1621 323
MspA1I CmG'CkG
Cuts at: 0 332 410 473 983 997 1827 1944
Size: 332 78 63 510 14 830 117
Fragments arranged by size:
830 510 332 117 78 63 14
NaeI GCC'GGC
Cuts at: 0 12 1944
Size: 12 1932
NarI GG'CG_CC
Cuts at: 0 1934 1944
Size: 1934 10
NgoMIV G'CCGG_C
Cuts at: 0 10 1944
Size: 10 1934
NspI r_CATG'y
Cuts at: 0 1227 1944
Size: 1227 717
PasI CC'CwG_GG
Cuts at: 0 339 1944
Size: 339 1605
PfoI T'CCnGG_A
Cuts at: 0 647 1439 1517 1598 1944
Size: 647 792 78 81 346
Fragments arranged by size:
792 647 346 81 78
PmlI CAC'GTG
Cuts at: 0 867 1944
Size: 867 1077
PpuMI rG'GwC_Cy
Cuts at: 0 894 1171 1944
Size: 894 277 773
Fragments arranged by size:
894 773 277
PspOMI G'GGCC_C
Cuts at: 0 831 870 1847 1944
Size: 831 39 977 97
Fragments arranged by size:
977 831 97 39
PstI C_TGCA'G
Cuts at: 0 605 716 890 1058 1944
Size: 605 111 174 168 886
Fragments arranged by size:
886 605 174 168 111
PvuII CAG'CTG
Cuts at: 0 332 410 983 997 1944
Size: 332 78 573 14 947
Fragments arranged by size:
947 573 332 78 14
SacI G_AGCT'C
Cuts at: 0 1340 1944
Size: 1340 604
SacII CC_GC'GG
Cuts at: 0 474 1944
Size: 474 1470
SanDI GG'GwC_CC
Cuts at: 0 1171 1944
Size: 1171 773
SexAI A'CCwGG_T
Cuts at: 0 419 1944
Size: 419 1525
SfcI C'TryA_G
Cuts at: 0 15 149 601 712 886 1054 1944
Size: 15 134 452 111 174 168 890
Fragments arranged by size:
890 452 174 168 134 111 15
SfoI GGC'GCC
Cuts at: 0 1935 1944
Size: 1935 9
SmaI CCC'GGG
Cuts at: 0 1184 1846 1944
Size: 1184 662 98
Fragments arranged by size:
1184 662 98
SmlI C'TyrA_G
Cuts at: 0 1654 1944
Size: 1654 290
StuI AGG'CCT
Cuts at: 0 404 955 1944
Size: 404 551 989
Fragments arranged by size:
989 551 404
StyI C'CwwG_G
Cuts at: 0 969 1944
Size: 969 975
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 92 187 1944
Size: 92 95 1757
Fragments arranged by size:
1757 95 92
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 449 481 629 661 1531 1563 1944
Size: 449 32 148 32 870 32 381
Fragments arranged by size:
870 449 381 148 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 407 1602 1944
Size: 407 1195 342
Fragments arranged by size:
1195 407 342
XmaI C'CCGG_G
Cuts at: 0 1182 1844 1944
Size: 1182 662 100
Fragments arranged by size:
1182 662 100
ZraI GAC'GTC
Cuts at: 0 129 1944
Size: 129 1815
Enzymes that do cut:
AarI AatII Acc65I AcuI AfeI AlfI ApaI ApoI
AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BglI BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI BsrBI BsrFI
BssSI BstEII BstYI Bsu36I BtgI BtgZI BtsI DraIII
DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV HaeII Hin4I KasI KpnI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PasI PfoI
PmlI PpuMI PspOMI PstI PvuII SacI SacII SanDI
SexAI SfcI SfoI SmaI SmlI StuI StyI TaqII
TstI XcmI XmaI ZraI
Enzymes that do not cut:
AccI AclI AflII AflIII AgeI AhdI AleI AloI
AlwNI ApaLI AscI AseI AsiSI BaeI BclI BfrBI
BglII BmtI BsaBI BsiEI BsiWI BsmI BsmBI BspEI
BspHI BsrDI BsrGI BssHII BstAPI BstBI BstXI BstZ17I
ClaI CspCI DraI EagI FalI FseI FspI FspAI
HincII HindIII HpaI MfeI MluI NcoI NdeI NheI
NotI NruI NsiI PacI PciI PflMI PmeI PpiI
PshAI PsiI PspXI PsrI PvuI RsrII SalI SapI
SbfI ScaI SfiI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI SwaI TatI Tth111I XbaI XhoI XmnI