Restriction map for
pFN21AE2037
(Linear) (Six-base) MAPSORT of: KIEE2037.seq Check: 2081 from: 1 to: 1902
KIEE2037.orf
With 182 enzymes: SgfI *
July 15, 2010 18:53 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1024 1902
Size: 1024 878
AccI GT'mk_AC
Cuts at: 0 1700 1902
Size: 1700 202
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 168 1902
Size: 168 1734
AflII C'TTAA_G
Cuts at: 0 1773 1839 1902
Size: 1773 66 63
Fragments arranged by size:
1773 66 63
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 671 1902
Size: 671 1231
ApaI G_GGCC'C
Cuts at: 0 118 1902
Size: 118 1784
ApoI r'AATT_y
Cuts at: 0 390 1902
Size: 390 1512
AseI AT'TA_AT
Cuts at: 0 1061 1902
Size: 1061 841
AvaI C'yCGr_G
Cuts at: 0 106 1902
Size: 106 1796
BamHI G'GATC_C
Cuts at: 0 3 1902
Size: 3 1899
BanI G'GyrC_C
Cuts at: 0 69 263 295 342 919 1902
Size: 69 194 32 47 577 983
Fragments arranged by size:
983 577 194 69 47 32
BanII G_rGCy'C
Cuts at: 0 118 1902
Size: 118 1784
BbeI G_GCGC'C
Cuts at: 0 73 299 1902
Size: 73 226 1603
Fragments arranged by size:
1603 226 73
BbvCI CC'TCA_GC
Cuts at: 0 172 1902
Size: 172 1730
BfrBI ATG'CAT
Cuts at: 0 1763 1902
Size: 1763 139
BglI GCCn_nnn'nGGC
Cuts at: 0 122 1902
Size: 122 1780
Bme1580I G_kGCm'C
Cuts at: 0 118 224 268 546 650 1348 1902
Size: 118 106 44 278 104 698 554
Fragments arranged by size:
698 554 278 118 106 104 44
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1225 1902
Size: 1225 677
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 437 1220 1902
Size: 437 783 682
Fragments arranged by size:
783 682 437
Bpu10I CC'TnA_GC
Cuts at: 0 172 1188 1902
Size: 172 1016 714
Fragments arranged by size:
1016 714 172
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 344 911 1819 1902
Size: 344 567 908 83
Fragments arranged by size:
908 567 344 83
BsaI GGTCTCn'nnnn_
Cuts at: 0 1795 1902
Size: 1795 107
BsaAI yAC'GTr
Cuts at: 0 651 1902
Size: 651 1251
BsaHI Gr'CG_yC
Cuts at: 0 70 296 1902
Size: 70 226 1606
Fragments arranged by size:
1606 226 70
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 574 1534 1681 1902
Size: 574 960 147 221
Fragments arranged by size:
960 574 221 147
BseYI C'CCAG_C
Cuts at: 0 80 158 285 1902
Size: 80 78 127 1617
Fragments arranged by size:
1617 127 80 78
BsiEI CG_ry'CG
Cuts at: 0 152 1902
Size: 152 1750
BsiHKAI G_wGCw'C
Cuts at: 0 473 612 1610 1902
Size: 473 139 998 292
Fragments arranged by size:
998 473 292 139
Bsp1286I G_dGCh'C
Cuts at: 0 118 224 268 473 546 612 650 1348
Size: 118 106 44 205 73 66 38 698
Cuts at: 1348 1610 1902
Size: 262 292
Fragments arranged by size:
698 292 262 205 118 106 73 66
44 38
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1024 1902
Size: 1024 878
BsrDI GCAATG_nn'
Cuts at: 0 1022 1101 1763 1859 1902
Size: 1022 79 662 96 43
Fragments arranged by size:
1022 662 96 79 43
BsrFI r'CCGG_y
Cuts at: 0 188 316 1902
Size: 188 128 1586
Fragments arranged by size:
1586 188 128
BstYI r'GATC_y
Cuts at: 0 3 1375 1902
Size: 3 1372 527
Fragments arranged by size:
1372 527 3
BtgI C'CryG_G
Cuts at: 0 309 1902
Size: 309 1593
BtsI GCAGTG_nn'
Cuts at: 0 18 1902
Size: 18 1884
DraI TTT'AAA
Cuts at: 0 1809 1902
Size: 1809 93
DraIII CAC_nnn'GTG
Cuts at: 0 1425 1902
Size: 1425 477
DrdI GACnn_nn'nnGTC
Cuts at: 0 447 1902
Size: 447 1455
EaeI y'GGCC_r
Cuts at: 0 149 885 1902
Size: 149 736 1017
Fragments arranged by size:
1017 736 149
EagI C'GGCC_G
Cuts at: 0 149 1902
Size: 149 1753
EarI CTCTTCn'nnn_
Cuts at: 0 560 1511 1902
Size: 560 951 391
Fragments arranged by size:
951 560 391
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 106 1902
Size: 106 1796
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 168 437 1220 1902
Size: 168 269 783 682
Fragments arranged by size:
783 682 269 168
EcoO109I rG'GnC_Cy
Cuts at: 0 115 1902
Size: 115 1787
HaeII r_GCGC'y
Cuts at: 0 73 277 299 1902
Size: 73 204 22 1603
Fragments arranged by size:
1603 204 73 22
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 355 387 1059 1091 1597 1629 1693 1725
Size: 355 32 672 32 506 32 64 32
Cuts at: 1725 1902
Size: 177
Fragments arranged by size:
672 506 355 177 64 32 32 32
32
HincII GTy'rAC
Cuts at: 0 204 668 708 1902
Size: 204 464 40 1194
Fragments arranged by size:
1194 464 204 40
KasI G'GCGC_C
Cuts at: 0 69 295 1902
Size: 69 226 1607
Fragments arranged by size:
1607 226 69
MfeI C'AATT_G
Cuts at: 0 1319 1865 1902
Size: 1319 546 37
Fragments arranged by size:
1319 546 37
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1102 1202 1902
Size: 1102 100 700
Fragments arranged by size:
1102 700 100
MscI TGG'CCA
Cuts at: 0 887 1902
Size: 887 1015
MslI CAynn'nnrTG
Cuts at: 0 629 653 1902
Size: 629 24 1249
Fragments arranged by size:
1249 629 24
MspA1I CmG'CkG
Cuts at: 0 47 162 289 323 397 1902
Size: 47 115 127 34 74 1505
Fragments arranged by size:
1505 127 115 74 47 34
NaeI GCC'GGC
Cuts at: 0 190 318 1902
Size: 190 128 1584
Fragments arranged by size:
1584 190 128
NarI GG'CG_CC
Cuts at: 0 70 296 1902
Size: 70 226 1606
Fragments arranged by size:
1606 226 70
NgoMIV G'CCGG_C
Cuts at: 0 188 316 1902
Size: 188 128 1586
Fragments arranged by size:
1586 188 128
NsiI A_TGCA'T
Cuts at: 0 1765 1902
Size: 1765 137
NspI r_CATG'y
Cuts at: 0 628 1015 1902
Size: 628 387 887
Fragments arranged by size:
887 628 387
PflMI CCAn_nnn'nTGG
Cuts at: 0 505 1902
Size: 505 1397
PspOMI G'GGCC_C
Cuts at: 0 114 1902
Size: 114 1788
PvuII CAG'CTG
Cuts at: 0 289 397 1902
Size: 289 108 1505
Fragments arranged by size:
1505 289 108
SfoI GGC'GCC
Cuts at: 0 71 297 1902
Size: 71 226 1605
Fragments arranged by size:
1605 226 71
SmaI CCC'GGG
Cuts at: 0 108 1902
Size: 108 1794
SmlI C'TyrA_G
Cuts at: 0 359 926 1773 1798 1839 1902
Size: 359 567 847 25 41 63
Fragments arranged by size:
847 567 359 63 41 25
SpeI A'CTAG_T
Cuts at: 0 1125 1902
Size: 1125 777
StyI C'CwwG_G
Cuts at: 0 512 1234 1902
Size: 512 722 668
Fragments arranged by size:
722 668 512
TatI w'GTAC_w
Cuts at: 0 1331 1902
Size: 1331 571
XbaI T'CTAG_A
Cuts at: 0 873 1902
Size: 873 1029
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 461 1085 1902
Size: 461 624 817
Fragments arranged by size:
817 624 461
XmaI C'CCGG_G
Cuts at: 0 106 1902
Size: 106 1796
XmnI GAAnn'nnTTC
Cuts at: 0 862 1443 1902
Size: 862 581 459
Fragments arranged by size:
862 581 459
Enzymes that do cut:
AarI AccI AcuI AflII AlfI ApaI ApoI AseI
AvaI BamHI BanI BanII BbeI BbvCI BfrBI BglI
Bme1580I BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI
BseRI BseYI BsiEI BsiHKAI Bsp1286I BspMI BsrDI BsrFI
BstYI BtgI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I HaeII Hin4I HincII KasI
MfeI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NsiI NspI PflMI PspOMI PvuII SfoI SmaI SmlI
SpeI StyI TatI XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflIII AgeI AhdI AleI
AloI AlwNI ApaLI AscI AsiSI AvrII BaeI BbsI
BcgI BciVI BclI BglII BlpI BmgBI BmrI BmtI
BsaBI BsaWI BsaXI BsgI BsiWI BsmI BsmBI BspEI
BspHI BsrBI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstZ17I Bsu36I BtgZI ClaI CspCI EcoICRI EcoNI
EcoRI EcoRV FalI FseI FspI FspAI HindIII HpaI
KpnI MluI NcoI NdeI NheI NotI NruI PacI
PasI PciI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspXI PsrI PstI PvuI RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI
SgfI SgrAI SnaBI SphI SrfI SspI StuI SwaI
TaqII TstI Tth111I XhoI ZraI