Restriction map for
pFN21AE2035
(Linear) (Six-base) MAPSORT of: KIEE2035.seq Check: 8061 from: 1 to: 1887
KIEE2035.orf
With 182 enzymes: SgfI *
July 15, 2010 18:53 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 36 1887
Size: 36 1851
AflIII A'CryG_T
Cuts at: 0 480 1887
Size: 480 1407
AlwNI CAG_nnn'CTG
Cuts at: 0 1764 1887
Size: 1764 123
ApoI r'AATT_y
Cuts at: 0 632 805 841 996 1460 1467 1611 1777
Size: 632 173 36 155 464 7 144 166
Cuts at: 1777 1887
Size: 110
Fragments arranged by size:
632 464 173 166 155 144 110 36
7
AseI AT'TA_AT
Cuts at: 0 239 1823 1887
Size: 239 1584 64
Fragments arranged by size:
1584 239 64
BanI G'GyrC_C
Cuts at: 0 1031 1887
Size: 1031 856
Bme1580I G_kGCm'C
Cuts at: 0 707 1034 1887
Size: 707 327 853
Fragments arranged by size:
853 707 327
BmrI ACTGGGnnnn_n'
Cuts at: 0 1154 1733 1887
Size: 1154 579 154
Fragments arranged by size:
1154 579 154
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1393 1887
Size: 1393 494
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 510 1887
Size: 510 1377
BsaAI yAC'GTr
Cuts at: 0 474 870 1887
Size: 474 396 1017
Fragments arranged by size:
1017 474 396
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1067 1717 1822 1887
Size: 1067 650 105 65
Fragments arranged by size:
1067 650 105 65
BseYI C'CCAG_C
Cuts at: 0 442 1887
Size: 442 1445
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 285 1887
Size: 285 1602
BsmI GAATG_Cn'
Cuts at: 0 444 976 1675 1887
Size: 444 532 699 212
Fragments arranged by size:
699 532 444 212
Bsp1286I G_dGCh'C
Cuts at: 0 707 1034 1887
Size: 707 327 853
Fragments arranged by size:
853 707 327
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1266 1887
Size: 1266 621
BsrDI GCAATG_nn'
Cuts at: 0 1224 1887
Size: 1224 663
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1722 1887
Size: 1722 165
BstYI r'GATC_y
Cuts at: 0 1160 1887
Size: 1160 727
Bsu36I CC'TnA_GG
Cuts at: 0 1478 1887
Size: 1478 409
BtsI GCAGTG_nn'
Cuts at: 0 302 1887
Size: 302 1585
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 455 490 1887
Size: 455 35 1397
Fragments arranged by size:
1397 455 35
DraIII CAC_nnn'GTG
Cuts at: 0 1741 1887
Size: 1741 146
EaeI y'GGCC_r
Cuts at: 0 93 1887
Size: 93 1794
EarI CTCTTCn'nnn_
Cuts at: 0 55 481 500 608 1887
Size: 55 426 19 108 1279
Fragments arranged by size:
1279 426 108 55 19
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 359 1887
Size: 359 1528
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 36 1393 1887
Size: 36 1357 494
Fragments arranged by size:
1357 494 36
EcoO109I rG'GnC_Cy
Cuts at: 0 1278 1366 1887
Size: 1278 88 521
Fragments arranged by size:
1278 521 88
EcoRI G'AATT_C
Cuts at: 0 841 996 1611 1887
Size: 841 155 615 276
Fragments arranged by size:
841 615 276 155
EcoRV GAT'ATC
Cuts at: 0 367 1887
Size: 367 1520
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 591 1887
Size: 591 1296
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 212 244 1801 1833 1887
Size: 212 32 1557 32 54
Fragments arranged by size:
1557 212 54 32 32
MfeI C'AATT_G
Cuts at: 0 740 1887
Size: 740 1147
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1005 1887
Size: 1005 882
MscI TGG'CCA
Cuts at: 0 95 1887
Size: 95 1792
MslI CAynn'nnrTG
Cuts at: 0 339 641 1887
Size: 339 302 1246
Fragments arranged by size:
1246 339 302
MspA1I CmG'CkG
Cuts at: 0 56 1887
Size: 56 1831
NdeI CA'TA_TG
Cuts at: 0 825 1887
Size: 825 1062
NspI r_CATG'y
Cuts at: 0 289 484 1887
Size: 289 195 1403
Fragments arranged by size:
1403 289 195
PciI A'CATG_T
Cuts at: 0 480 1887
Size: 480 1407
PpuMI rG'GwC_Cy
Cuts at: 0 1278 1366 1887
Size: 1278 88 521
Fragments arranged by size:
1278 521 88
PsiI TTA'TAA
Cuts at: 0 200 1887
Size: 200 1687
PstI C_TGCA'G
Cuts at: 0 666 1887
Size: 666 1221
PvuII CAG'CTG
Cuts at: 0 56 1887
Size: 56 1831
SapI GCTCTTCn'nnn_
Cuts at: 0 500 1887
Size: 500 1387
ScaI AGT'ACT
Cuts at: 0 574 1887
Size: 574 1313
SfcI C'TryA_G
Cuts at: 0 662 698 1025 1234 1340 1415 1887
Size: 662 36 327 209 106 75 472
Fragments arranged by size:
662 472 327 209 106 75 36
SmlI C'TyrA_G
Cuts at: 0 525 1887
Size: 525 1362
SnaBI TAC'GTA
Cuts at: 0 870 1887
Size: 870 1017
SspI AAT'ATT
Cuts at: 0 412 1631 1845 1887
Size: 412 1219 214 42
Fragments arranged by size:
1219 412 214 42
StyI C'CwwG_G
Cuts at: 0 181 916 1887
Size: 181 735 971
Fragments arranged by size:
971 735 181
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1752 1887
Size: 1752 135
TatI w'GTAC_w
Cuts at: 0 296 572 1887
Size: 296 276 1315
Fragments arranged by size:
1315 296 276
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1719 1887
Size: 1719 168
XmnI GAAnn'nnTTC
Cuts at: 0 221 953 1196 1748 1887
Size: 221 732 243 552 139
Fragments arranged by size:
732 552 243 221 139
Enzymes that do cut:
AcuI AflIII AlwNI ApoI AseI BanI Bme1580I BmrI
BpmI BpuEI BsaAI BseRI BseYI BsgI BsmI Bsp1286I
BspMI BsrDI BstXI BstYI Bsu36I BtsI CspCI DraIII
EaeI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI
Hin4I MfeI MmeI MscI MslI MspA1I NdeI NspI
PciI PpuMI PsiI PstI PvuII SapI ScaI SfcI
SmlI SnaBI SspI StyI TaqII TatI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI AloI ApaI ApaLI AscI AsiSI
AvaI AvrII BaeI BamHI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI BmgBI
BmtI BplI Bpu10I BsaI BsaBI BsaHI BsaWI BsaXI
BsiEI BsiHKAI BsiWI BsmBI BspEI BspHI BsrBI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I BtgI
BtgZI ClaI DraI DrdI EagI EcoICRI EcoNI FseI
FspI FspAI HaeII HincII HindIII HpaI KasI KpnI
MluI NaeI NarI NcoI NgoMIV NheI NotI NruI
NsiI PacI PasI PflMI PfoI PmeI PmlI PpiI
PshAI PspOMI PspXI PsrI PvuI RsrII SacI SacII
SalI SanDI SbfI SexAI SfiI SfoI SgfI SgrAI
SmaI SpeI SphI SrfI StuI SwaI TstI Tth111I
XbaI XhoI XmaI ZraI