Restriction map for
pFN21AE2034
(Linear) (Six-base) MAPSORT of: KIEE2034.seq Check: 5862 from: 1 to: 1881
KIEE2034.orf
With 182 enzymes: SgfI *
July 15, 2010 18:53 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1679 1881
Size: 1679 202
AatII G_ACGT'C
Cuts at: 0 494 1881
Size: 494 1387
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 142 1753 1881
Size: 142 1611 128
Fragments arranged by size:
1611 142 128
AflIII A'CryG_T
Cuts at: 0 1409 1881
Size: 1409 472
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 856 1881
Size: 856 1025
AlwNI CAG_nnn'CTG
Cuts at: 0 1200 1302 1881
Size: 1200 102 579
Fragments arranged by size:
1200 579 102
ApaI G_GGCC'C
Cuts at: 0 16 102 1881
Size: 16 86 1779
Fragments arranged by size:
1779 86 16
ApaLI G'TGCA_C
Cuts at: 0 988 1881
Size: 988 893
ApoI r'AATT_y
Cuts at: 0 532 1881
Size: 532 1349
AvaI C'yCGr_G
Cuts at: 0 95 1156 1481 1498 1623 1694 1881
Size: 95 1061 325 17 125 71 187
Fragments arranged by size:
1061 325 187 125 95 71 17
BanI G'GyrC_C
Cuts at: 0 646 1189 1360 1368 1448 1522 1881
Size: 646 543 171 8 80 74 359
Fragments arranged by size:
646 543 359 171 80 74 8
BanII G_rGCy'C
Cuts at: 0 16 102 350 478 1175 1559 1610 1881
Size: 16 86 248 128 697 384 51 271
Fragments arranged by size:
697 384 271 248 128 86 51 16
BbeI G_GCGC'C
Cuts at: 0 1526 1881
Size: 1526 355
BbsI GAAGACnn'nnnn_
Cuts at: 0 783 852 966 1032 1748 1881
Size: 783 69 114 66 716 133
Fragments arranged by size:
783 716 133 114 69 66
BbvCI CC'TCA_GC
Cuts at: 0 1292 1396 1881
Size: 1292 104 485
Fragments arranged by size:
1292 485 104
BfrBI ATG'CAT
Cuts at: 0 1125 1881
Size: 1125 756
BglI GCCn_nnn'nGGC
Cuts at: 0 1501 1863 1881
Size: 1501 362 18
Fragments arranged by size:
1501 362 18
BglII A'GATC_T
Cuts at: 0 683 1881
Size: 683 1198
BlpI GC'TnA_GC
Cuts at: 0 212 1247 1553 1881
Size: 212 1035 306 328
Fragments arranged by size:
1035 328 306 212
Bme1580I G_kGCm'C
Cuts at: 0 16 102 649 992 1143 1881
Size: 16 86 547 343 151 738
Fragments arranged by size:
738 547 343 151 86 16
BmgBI CAC'GTC
Cuts at: 0 180 225 1881
Size: 180 45 1656
Fragments arranged by size:
1656 180 45
Bpu10I CC'TnA_GC
Cuts at: 0 1292 1396 1881
Size: 1292 104 485
Fragments arranged by size:
1292 485 104
BsaI GGTCTCn'nnnn_
Cuts at: 0 85 1460 1881
Size: 85 1375 421
Fragments arranged by size:
1375 421 85
BsaAI yAC'GTr
Cuts at: 0 1779 1881
Size: 1779 102
BsaBI GATnn'nnATC
Cuts at: 0 1816 1881
Size: 1816 65
BsaHI Gr'CG_yC
Cuts at: 0 491 638 1442 1523 1709 1881
Size: 491 147 804 81 186 172
Fragments arranged by size:
804 491 186 172 147 81
BsaWI w'CCGG_w
Cuts at: 0 136 1445 1881
Size: 136 1309 436
Fragments arranged by size:
1309 436 136
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 460 490 787 817 1049 1079 1881
Size: 460 30 297 30 232 30 802
Fragments arranged by size:
802 460 297 232 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 124 1057 1280 1881
Size: 124 933 223 601
Fragments arranged by size:
933 601 223 124
BseYI C'CCAG_C
Cuts at: 0 296 303 1833 1881
Size: 296 7 1530 48
Fragments arranged by size:
1530 296 48 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 466 507 1186 1881
Size: 466 41 679 695
Fragments arranged by size:
695 679 466 41
BsiEI CG_ry'CG
Cuts at: 0 361 1081 1797 1881
Size: 361 720 716 84
Fragments arranged by size:
720 716 361 84
BsiHKAI G_wGCw'C
Cuts at: 0 350 478 983 992 1559 1881
Size: 350 128 505 9 567 322
Fragments arranged by size:
567 505 350 322 128 9
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1478 1881
Size: 1478 403
Bsp1286I G_dGCh'C
Cuts at: 0 16 102 350 478 649 983 992 1143
Size: 16 86 248 128 171 334 9 151
Cuts at: 1143 1175 1559 1610 1881
Size: 32 384 51 271
Fragments arranged by size:
384 334 271 248 171 151 128 86
51 32 16 9
BspHI T'CATG_A
Cuts at: 0 1070 1881
Size: 1070 811
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 806 935 1679 1881
Size: 806 129 744 202
Fragments arranged by size:
806 744 202 129
BsrBI CCG'CTC
Cuts at: 0 114 729 998 1187 1881
Size: 114 615 269 189 694
Fragments arranged by size:
694 615 269 189 114
BsrDI GCAATG_nn'
Cuts at: 0 1718 1881
Size: 1718 163
BsrFI r'CCGG_y
Cuts at: 0 61 1103 1242 1451 1519 1683 1881
Size: 61 1042 139 209 68 164 198
Fragments arranged by size:
1042 209 198 164 139 68 61
BssHII G'CGCG_C
Cuts at: 0 72 1881
Size: 72 1809
BssSI C'ACGA_G
Cuts at: 0 1182 1881
Size: 1182 699
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1017 1881
Size: 1017 864
BstEII G'GTnAC_C
Cuts at: 0 402 1881
Size: 402 1479
BstXI CCAn_nnnn'nTGG
Cuts at: 0 900 1881
Size: 900 981
BstYI r'GATC_y
Cuts at: 0 683 1881
Size: 683 1198
BtgI C'CryG_G
Cuts at: 0 25 161 641 1085 1356 1631 1784 1881
Size: 25 136 480 444 271 275 153 97
Fragments arranged by size:
480 444 275 271 153 136 97 25
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 192 1881
Size: 192 1689
BtsI GCAGTG_nn'
Cuts at: 0 454 1881
Size: 454 1427
DraIII CAC_nnn'GTG
Cuts at: 0 830 1881
Size: 830 1051
DrdI GACnn_nn'nnGTC
Cuts at: 0 400 1881
Size: 400 1481
EaeI y'GGCC_r
Cuts at: 0 164 358 461 1078 1133 1379 1509 1781
Size: 164 194 103 617 55 246 130 272
Cuts at: 1781 1881
Size: 100
Fragments arranged by size:
617 272 246 194 164 130 103 100
55
EagI C'GGCC_G
Cuts at: 0 358 1078 1881
Size: 358 720 803
Fragments arranged by size:
803 720 358
EarI CTCTTCn'nnn_
Cuts at: 0 136 736 1617 1855 1881
Size: 136 600 881 238 26
Fragments arranged by size:
881 600 238 136 26
EcoICRI GAG'CTC
Cuts at: 0 348 476 1557 1881
Size: 348 128 1081 324
Fragments arranged by size:
1081 348 324 128
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 142 1753 1881
Size: 142 1611 128
Fragments arranged by size:
1611 142 128
EcoNI CCTnn'n_nnAGG
Cuts at: 0 415 1881
Size: 415 1466
EcoO109I rG'GnC_Cy
Cuts at: 0 12 13 300 1391 1881
Size: 12 1 287 1091 490
Fragments arranged by size:
1091 490 287 12 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 147 1881
Size: 147 1734
HaeII r_GCGC'y
Cuts at: 0 25 1526 1881
Size: 25 1501 355
Fragments arranged by size:
1501 355 25
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1561 1593 1881
Size: 1561 32 288
Fragments arranged by size:
1561 288 32
KasI G'GCGC_C
Cuts at: 0 1522 1881
Size: 1522 359
MfeI C'AATT_G
Cuts at: 0 1098 1881
Size: 1098 783
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1301 1881
Size: 1301 580
MscI TGG'CCA
Cuts at: 0 166 1135 1381 1511 1783 1881
Size: 166 969 246 130 272 98
Fragments arranged by size:
969 272 246 166 130 98
MslI CAynn'nnrTG
Cuts at: 0 223 838 1881
Size: 223 615 1043
Fragments arranged by size:
1043 615 223
MspA1I CmG'CkG
Cuts at: 0 27 42 307 600 1881
Size: 27 15 265 293 1281
Fragments arranged by size:
1281 293 265 27 15
NaeI GCC'GGC
Cuts at: 0 1105 1244 1521 1685 1881
Size: 1105 139 277 164 196
Fragments arranged by size:
1105 277 196 164 139
NarI GG'CG_CC
Cuts at: 0 1523 1881
Size: 1523 358
NcoI C'CATG_G
Cuts at: 0 1784 1881
Size: 1784 97
NgoMIV G'CCGG_C
Cuts at: 0 1103 1242 1519 1683 1881
Size: 1103 139 277 164 198
Fragments arranged by size:
1103 277 198 164 139
NsiI A_TGCA'T
Cuts at: 0 1127 1881
Size: 1127 754
NspI r_CATG'y
Cuts at: 0 582 1413 1598 1881
Size: 582 831 185 283
Fragments arranged by size:
831 582 283 185
PciI A'CATG_T
Cuts at: 0 1409 1881
Size: 1409 472
PfoI T'CCnGG_A
Cuts at: 0 541 1043 1881
Size: 541 502 838
Fragments arranged by size:
838 541 502
PpuMI rG'GwC_Cy
Cuts at: 0 300 1391 1881
Size: 300 1091 490
Fragments arranged by size:
1091 490 300
PspOMI G'GGCC_C
Cuts at: 0 12 98 1881
Size: 12 86 1783
Fragments arranged by size:
1783 86 12
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 916 948 1881
Size: 916 32 933
Fragments arranged by size:
933 916 32
PstI C_TGCA'G
Cuts at: 0 484 1881
Size: 484 1397
PvuII CAG'CTG
Cuts at: 0 307 600 1881
Size: 307 293 1281
Fragments arranged by size:
1281 307 293
SacI G_AGCT'C
Cuts at: 0 350 478 1559 1881
Size: 350 128 1081 322
Fragments arranged by size:
1081 350 322 128
SacII CC_GC'GG
Cuts at: 0 28 1881
Size: 28 1853
SanDI GG'GwC_CC
Cuts at: 0 300 1881
Size: 300 1581
SapI GCTCTTCn'nnn_
Cuts at: 0 736 1881
Size: 736 1145
ScaI AGT'ACT
Cuts at: 0 1471 1881
Size: 1471 410
SexAI A'CCwGG_T
Cuts at: 0 569 1881
Size: 569 1312
SfcI C'TryA_G
Cuts at: 0 480 1881
Size: 480 1401
SfoI GGC'GCC
Cuts at: 0 1524 1881
Size: 1524 357
SmaI CCC'GGG
Cuts at: 0 97 1696 1881
Size: 97 1599 185
Fragments arranged by size:
1599 185 97
StyI C'CwwG_G
Cuts at: 0 416 1784 1881
Size: 416 1368 97
Fragments arranged by size:
1368 416 97
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 252 421 1207 1378 1558 1881
Size: 252 169 786 171 180 323
Fragments arranged by size:
786 323 252 180 171 169
TatI w'GTAC_w
Cuts at: 0 1469 1715 1881
Size: 1469 246 166
Fragments arranged by size:
1469 246 166
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 890 922 1049 1081 1881
Size: 890 32 127 32 800
Fragments arranged by size:
890 800 127 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 181 367 1881
Size: 181 186 1514
Fragments arranged by size:
1514 186 181
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 84 567 1128 1333 1881
Size: 84 483 561 205 548
Fragments arranged by size:
561 548 483 205 84
XmaI C'CCGG_G
Cuts at: 0 95 1694 1881
Size: 95 1599 187
Fragments arranged by size:
1599 187 95
XmnI GAAnn'nnTTC
Cuts at: 0 130 1881
Size: 130 1751
ZraI GAC'GTC
Cuts at: 0 492 1881
Size: 492 1389
Enzymes that do cut:
AarI AatII AcuI AflIII AlfI AlwNI ApaI ApaLI
ApoI AvaI BanI BanII BbeI BbsI BbvCI BfrBI
BglI BglII BlpI Bme1580I BmgBI Bpu10I BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstEII BstXI BstYI BtgI BtgZI
BtsI DraIII DrdI EaeI EagI EarI EcoICRI Eco57MI
EcoNI EcoO109I FalI HaeII Hin4I KasI MfeI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI
NspI PciI PfoI PpuMI PspOMI PsrI PstI PvuII
SacI SacII SanDI SapI ScaI SexAI SfcI SfoI
SmaI StyI TaqII TatI TstI Tth111I XcmI XmaI
XmnI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AfeI AflII AgeI AhdI AleI
AloI AscI AseI AsiSI AvrII BaeI BamHI BcgI
BciVI BclI BmrI BmtI BplI BpmI BpuEI BsiWI
BsmI BspEI BsrGI BstBI BstZ17I Bsu36I ClaI CspCI
DraI EciI EcoRI EcoRV FseI FspI FspAI HincII
HindIII HpaI KpnI MluI NdeI NheI NotI NruI
PacI PasI PflMI PmeI PmlI PpiI PshAI PsiI
PspXI PvuI RsrII SalI SbfI SfiI SgfI SgrAI
SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI
XbaI XhoI