Restriction map for
pFN21AE2033
(Linear) (Six-base) MAPSORT of: KIEE2033.seq Check: 1566 from: 1 to: 1872
KIEE2033.orf
With 182 enzymes: SgfI *
July 15, 2010 18:53 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 677 1872
Size: 677 1195
AccI GT'mk_AC
Cuts at: 0 379 1872
Size: 379 1493
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1421 1872
Size: 1421 451
AfeI AGC'GCT
Cuts at: 0 1620 1872
Size: 1620 252
AlwNI CAG_nnn'CTG
Cuts at: 0 1113 1194 1657 1698 1872
Size: 1113 81 463 41 174
Fragments arranged by size:
1113 463 174 81 41
ApaI G_GGCC'C
Cuts at: 0 1544 1872
Size: 1544 328
AvaI C'yCGr_G
Cuts at: 0 145 1872
Size: 145 1727
BanI G'GyrC_C
Cuts at: 0 141 960 1164 1872
Size: 141 819 204 708
Fragments arranged by size:
819 708 204 141
BanII G_rGCy'C
Cuts at: 0 481 500 1544 1861 1872
Size: 481 19 1044 317 11
Fragments arranged by size:
1044 481 317 19 11
BbeI G_GCGC'C
Cuts at: 0 1168 1872
Size: 1168 704
BbsI GAAGACnn'nnnn_
Cuts at: 0 118 1872
Size: 118 1754
BbvCI CC'TCA_GC
Cuts at: 0 1259 1872
Size: 1259 613
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1816 1850 1872
Size: 1816 34 22
Fragments arranged by size:
1816 34 22
BglI GCCn_nnn'nGGC
Cuts at: 0 1475 1502 1872
Size: 1475 27 370
Fragments arranged by size:
1475 370 27
BlpI GC'TnA_GC
Cuts at: 0 83 1195 1872
Size: 83 1112 677
Fragments arranged by size:
1112 677 83
Bme1580I G_kGCm'C
Cuts at: 0 146 963 1544 1872
Size: 146 817 581 328
Fragments arranged by size:
817 581 328 146
BmrI ACTGGGnnnn_n'
Cuts at: 0 1058 1474 1872
Size: 1058 416 398
Fragments arranged by size:
1058 416 398
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 171 1445 1872
Size: 171 1274 427
Fragments arranged by size:
1274 427 171
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 484 1415 1724 1872
Size: 484 931 309 148
Fragments arranged by size:
931 484 309 148
Bpu10I CC'TnA_GC
Cuts at: 0 460 1259 1872
Size: 460 799 613
Fragments arranged by size:
799 613 460
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 495 1872
Size: 495 1377
BsaBI GATnn'nnATC
Cuts at: 0 544 1872
Size: 544 1328
BsaHI Gr'CG_yC
Cuts at: 0 376 1165 1872
Size: 376 789 707
Fragments arranged by size:
789 707 376
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 145 888 1872
Size: 145 743 984
Fragments arranged by size:
984 743 145
BseYI C'CCAG_C
Cuts at: 0 1139 1290 1452 1854 1862 1872
Size: 1139 151 162 402 8 10
Fragments arranged by size:
1139 402 162 151 10 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1202 1607 1711 1872
Size: 1202 405 104 161
Fragments arranged by size:
1202 405 161 104
BsiHKAI G_wGCw'C
Cuts at: 0 481 500 1872
Size: 481 19 1372
Fragments arranged by size:
1372 481 19
BsmI GAATG_Cn'
Cuts at: 0 846 1872
Size: 846 1026
Bsp1286I G_dGCh'C
Cuts at: 0 146 481 500 963 1544 1861 1872
Size: 146 335 19 463 581 317 11
Fragments arranged by size:
581 463 335 317 146 19 11
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 677 1872
Size: 677 1195
BsrDI GCAATG_nn'
Cuts at: 0 354 1047 1358 1778 1872
Size: 354 693 311 420 94
Fragments arranged by size:
693 420 354 311 94
BsrFI r'CCGG_y
Cuts at: 0 1582 1872
Size: 1582 290
BsrGI T'GTAC_A
Cuts at: 0 812 1872
Size: 812 1060
BssSI C'ACGA_G
Cuts at: 0 1638 1872
Size: 1638 234
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1113 1308 1689 1872
Size: 1113 195 381 183
Fragments arranged by size:
1113 381 195 183
BstBI TT'CG_AA
Cuts at: 0 184 586 1872
Size: 184 402 1286
Fragments arranged by size:
1286 402 184
BstYI r'GATC_y
Cuts at: 0 1370 1872
Size: 1370 502
Bsu36I CC'TnA_GG
Cuts at: 0 323 1872
Size: 323 1549
BtgI C'CryG_G
Cuts at: 0 42 353 881 955 1598 1872
Size: 42 311 528 74 643 274
Fragments arranged by size:
643 528 311 274 74 42
DraIII CAC_nnn'GTG
Cuts at: 0 1630 1872
Size: 1630 242
EaeI y'GGCC_r
Cuts at: 0 527 1279 1872
Size: 527 752 593
Fragments arranged by size:
752 593 527
EcoICRI GAG'CTC
Cuts at: 0 479 498 1872
Size: 479 19 1374
Fragments arranged by size:
1374 479 19
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 484 1415 1421 1724 1872
Size: 484 931 6 303 148
Fragments arranged by size:
931 484 303 148 6
EcoO109I rG'GnC_Cy
Cuts at: 0 1468 1495 1504 1531 1549 1603 1695 1806
Size: 1468 27 9 27 18 54 92 111
Cuts at: 1806 1872
Size: 66
Fragments arranged by size:
1468 111 92 66 54 27 27 18
9
HaeII r_GCGC'y
Cuts at: 0 1168 1622 1872
Size: 1168 454 250
Fragments arranged by size:
1168 454 250
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 732 764 1188 1220 1299 1312 1331 1344
Size: 732 32 424 32 79 13 19 13
Cuts at: 1344 1814 1846 1872
Size: 470 32 26
Fragments arranged by size:
732 470 424 79 32 32 32 26
19 13 13
HincII GTy'rAC
Cuts at: 0 380 1872
Size: 380 1492
KasI G'GCGC_C
Cuts at: 0 1164 1872
Size: 1164 708
MfeI C'AATT_G
Cuts at: 0 637 1872
Size: 637 1235
MscI TGG'CCA
Cuts at: 0 529 1872
Size: 529 1343
MspA1I CmG'CkG
Cuts at: 0 23 44 355 1143 1242 1263 1722 1872
Size: 23 21 311 788 99 21 459 150
Fragments arranged by size:
788 459 311 150 99 23 21 21
NarI GG'CG_CC
Cuts at: 0 1165 1872
Size: 1165 707
NcoI C'CATG_G
Cuts at: 0 955 1872
Size: 955 917
NruI TCG'CGA
Cuts at: 0 938 1872
Size: 938 934
NspI r_CATG'y
Cuts at: 0 582 813 1107 1872
Size: 582 231 294 765
Fragments arranged by size:
765 582 294 231
PflMI CCAn_nnn'nTGG
Cuts at: 0 555 956 1872
Size: 555 401 916
Fragments arranged by size:
916 555 401
PpuMI rG'GwC_Cy
Cuts at: 0 1549 1872
Size: 1549 323
PspOMI G'GGCC_C
Cuts at: 0 1540 1872
Size: 1540 332
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 351 383 1872
Size: 351 32 1489
Fragments arranged by size:
1489 351 32
PstI C_TGCA'G
Cuts at: 0 1268 1304 1566 1872
Size: 1268 36 262 306
Fragments arranged by size:
1268 306 262 36
PvuII CAG'CTG
Cuts at: 0 1143 1263 1722 1872
Size: 1143 120 459 150
Fragments arranged by size:
1143 459 150 120
SacI G_AGCT'C
Cuts at: 0 481 500 1872
Size: 481 19 1372
Fragments arranged by size:
1372 481 19
SacII CC_GC'GG
Cuts at: 0 45 356 1872
Size: 45 311 1516
Fragments arranged by size:
1516 311 45
SalI G'TCGA_C
Cuts at: 0 378 1872
Size: 378 1494
SfcI C'TryA_G
Cuts at: 0 1264 1300 1384 1463 1472 1508 1562 1872
Size: 1264 36 84 79 9 36 54 310
Fragments arranged by size:
1264 310 84 79 54 36 36 9
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1475 1502 1872
Size: 1475 27 370
Fragments arranged by size:
1475 370 27
SfoI GGC'GCC
Cuts at: 0 1166 1872
Size: 1166 706
SmlI C'TyrA_G
Cuts at: 0 474 1872
Size: 474 1398
SphI G_CATG'C
Cuts at: 0 582 1107 1872
Size: 582 525 765
Fragments arranged by size:
765 582 525
StuI AGG'CCT
Cuts at: 0 458 1872
Size: 458 1414
StyI C'CwwG_G
Cuts at: 0 60 955 1872
Size: 60 895 917
Fragments arranged by size:
917 895 60
TatI w'GTAC_w
Cuts at: 0 812 1872
Size: 812 1060
XbaI T'CTAG_A
Cuts at: 0 771 1872
Size: 771 1101
XmnI GAAnn'nnTTC
Cuts at: 0 190 1872
Size: 190 1682
Enzymes that do cut:
AarI AccI AcuI AfeI AlwNI ApaI AvaI BanI
BanII BbeI BbsI BbvCI BcgI BglI BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaBI BsaHI BseRI
BseYI BsgI BsiHKAI BsmI Bsp1286I BspMI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstYI Bsu36I BtgI DraIII
EaeI EcoICRI Eco57MI EcoO109I HaeII Hin4I HincII KasI
MfeI MscI MspA1I NarI NcoI NruI NspI PflMI
PpuMI PspOMI PsrI PstI PvuII SacI SacII SalI
SfcI SfiI SfoI SmlI SphI StuI StyI TatI
XbaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AflII AflIII AgeI AhdI AleI
AlfI AloI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BciVI BclI BfrBI BglII BmgBI BmtI
BsaI BsaAI BsaWI BsaXI BsiEI BsiWI BsmBI BspEI
BspHI BsrBI BssHII BstEII BstXI BstZ17I BtgZI BtsI
ClaI CspCI DraI DrdI EagI EarI EciI EcoNI
EcoRI EcoRV FalI FseI FspI FspAI HindIII HpaI
KpnI MluI MmeI MslI NaeI NdeI NgoMIV NheI
NotI NsiI PacI PasI PciI PfoI PmeI PmlI
PpiI PshAI PsiI PspXI PvuI RsrII SanDI SapI
SbfI ScaI SexAI SgfI SgrAI SmaI SnaBI SpeI
SrfI SspI SwaI TaqII TstI Tth111I XcmI XhoI
XmaI ZraI