Restriction map for
pFN21AE2031
(Linear) (Six-base) MAPSORT of: KIEE2031.seq Check: 7905 from: 1 to: 1863
KIEE2031.orf
With 182 enzymes: SgfI *
July 15, 2010 18:53 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1419 1510 1797 1863
Size: 1419 91 287 66
Fragments arranged by size:
1419 287 91 66
AccI GT'mk_AC
Cuts at: 0 1088 1863
Size: 1088 775
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 940 1760 1863
Size: 940 820 103
Fragments arranged by size:
940 820 103
AfeI AGC'GCT
Cuts at: 0 1551 1863
Size: 1551 312
AleI CACnn'nnGTG
Cuts at: 0 268 1852 1863
Size: 268 1584 11
Fragments arranged by size:
1584 268 11
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1222 1254 1863
Size: 1222 32 609
Fragments arranged by size:
1222 609 32
AlwNI CAG_nnn'CTG
Cuts at: 0 540 814 1719 1863
Size: 540 274 905 144
Fragments arranged by size:
905 540 274 144
ApaI G_GGCC'C
Cuts at: 0 240 1863
Size: 240 1623
ApaLI G'TGCA_C
Cuts at: 0 1388 1813 1863
Size: 1388 425 50
Fragments arranged by size:
1388 425 50
ApoI r'AATT_y
Cuts at: 0 427 1863
Size: 427 1436
AvaI C'yCGr_G
Cuts at: 0 830 1397 1863
Size: 830 567 466
Fragments arranged by size:
830 567 466
BamHI G'GATC_C
Cuts at: 0 530 1574 1863
Size: 530 1044 289
Fragments arranged by size:
1044 530 289
BanII G_rGCy'C
Cuts at: 0 240 506 743 1040 1264 1863
Size: 240 266 237 297 224 599
Fragments arranged by size:
599 297 266 240 237 224
BbsI GAAGACnn'nnnn_
Cuts at: 0 713 1054 1863
Size: 713 341 809
Fragments arranged by size:
809 713 341
BbvCI CC'TCA_GC
Cuts at: 0 82 1276 1863
Size: 82 1194 587
Fragments arranged by size:
1194 587 82
BglI GCCn_nnn'nGGC
Cuts at: 0 1843 1863
Size: 1843 20
BglII A'GATC_T
Cuts at: 0 1233 1863
Size: 1233 630
BlpI GC'TnA_GC
Cuts at: 0 1553 1863
Size: 1553 310
Bme1580I G_kGCm'C
Cuts at: 0 13 240 1392 1817 1863
Size: 13 227 1152 425 46
Fragments arranged by size:
1152 425 227 46 13
BmgBI CAC'GTC
Cuts at: 0 246 720 1371 1863
Size: 246 474 651 492
Fragments arranged by size:
651 492 474 246
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 220 313 375 886 1063 1192 1801 1863
Size: 220 93 62 511 177 129 609 62
Fragments arranged by size:
609 511 220 177 129 93 62 62
Bpu10I CC'TnA_GC
Cuts at: 0 82 1276 1863
Size: 82 1194 587
Fragments arranged by size:
1194 587 82
BsaI GGTCTCn'nnnn_
Cuts at: 0 100 172 426 829 1863
Size: 100 72 254 403 1034
Fragments arranged by size:
1034 403 254 100 72
BsaHI Gr'CG_yC
Cuts at: 0 317 986 1863
Size: 317 669 877
Fragments arranged by size:
877 669 317
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 130 160 828 858 1863
Size: 130 30 668 30 1005
Fragments arranged by size:
1005 668 130 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 484 1347 1696 1863
Size: 484 863 349 167
Fragments arranged by size:
863 484 349 167
BseYI C'CCAG_C
Cuts at: 0 1546 1863
Size: 1546 317
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 16 1102 1365 1522 1753 1771 1825 1863
Size: 16 1086 263 157 231 18 54 38
Fragments arranged by size:
1086 263 231 157 54 38 18 16
BsiEI CG_ry'CG
Cuts at: 0 269 380 692 1863
Size: 269 111 312 1171
Fragments arranged by size:
1171 312 269 111
BsiHKAI G_wGCw'C
Cuts at: 0 1392 1817 1863
Size: 1392 425 46
Fragments arranged by size:
1392 425 46
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1261 1660 1863
Size: 1261 399 203
Fragments arranged by size:
1261 399 203
Bsp1286I G_dGCh'C
Cuts at: 0 13 240 506 743 1040 1264 1392 1817
Size: 13 227 266 237 297 224 128 425
Cuts at: 1817 1863
Size: 46
Fragments arranged by size:
425 297 266 237 227 224 128 46
13
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 239 665 1419 1510 1797 1863
Size: 239 426 754 91 287 66
Fragments arranged by size:
754 426 287 239 91 66
BsrBI CCG'CTC
Cuts at: 0 259 1752 1863
Size: 259 1493 111
Fragments arranged by size:
1493 259 111
BsrFI r'CCGG_y
Cuts at: 0 212 300 1648 1863
Size: 212 88 1348 215
Fragments arranged by size:
1348 215 212 88
BssHII G'CGCG_C
Cuts at: 0 1701 1863
Size: 1701 162
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1500 1812 1863
Size: 1500 312 51
Fragments arranged by size:
1500 312 51
BstBI TT'CG_AA
Cuts at: 0 704 1863
Size: 704 1159
BstXI CCAn_nnnn'nTGG
Cuts at: 0 981 1854 1863
Size: 981 873 9
Fragments arranged by size:
981 873 9
BstYI r'GATC_y
Cuts at: 0 77 530 1233 1505 1574 1863
Size: 77 453 703 272 69 289
Fragments arranged by size:
703 453 289 272 77 69
BtgI C'CryG_G
Cuts at: 0 173 506 692 803 1031 1331 1863
Size: 173 333 186 111 228 300 532
Fragments arranged by size:
532 333 300 228 186 173 111
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 782 1863
Size: 782 1081
BtsI GCAGTG_nn'
Cuts at: 0 384 1505 1863
Size: 384 1121 358
Fragments arranged by size:
1121 384 358
DraI TTT'AAA
Cuts at: 0 126 1863
Size: 126 1737
DraIII CAC_nnn'GTG
Cuts at: 0 360 1803 1863
Size: 360 1443 60
Fragments arranged by size:
1443 360 60
DrdI GACnn_nn'nnGTC
Cuts at: 0 418 1600 1863
Size: 418 1182 263
Fragments arranged by size:
1182 418 263
EaeI y'GGCC_r
Cuts at: 0 50 206 689 1328 1466 1588 1844 1863
Size: 50 156 483 639 138 122 256 19
Fragments arranged by size:
639 483 256 156 138 122 50 19
EagI C'GGCC_G
Cuts at: 0 689 1863
Size: 689 1174
EarI CTCTTCn'nnn_
Cuts at: 0 1681 1863
Size: 1681 182
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 678 1243 1350 1863
Size: 678 565 107 513
Fragments arranged by size:
678 565 513 107
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 220 313 375 886 940 1063 1192 1760
Size: 220 93 62 511 54 123 129 568
Cuts at: 1760 1801 1863
Size: 41 62
Fragments arranged by size:
568 511 220 129 123 93 62 62
54 41
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1678 1863
Size: 1678 185
EcoO109I rG'GnC_Cy
Cuts at: 0 195 237 537 654 897 1536 1863
Size: 195 42 300 117 243 639 327
Fragments arranged by size:
639 327 300 243 195 117 42
EcoRI G'AATT_C
Cuts at: 0 427 1863
Size: 427 1436
FseI GG_CCGG'CC
Cuts at: 0 1652 1863
Size: 1652 211
HaeII r_GCGC'y
Cuts at: 0 59 1553 1863
Size: 59 1494 310
Fragments arranged by size:
1494 310 59
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 130 162 828 860 1863
Size: 130 32 666 32 1003
Fragments arranged by size:
1003 666 130 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1503 1863
Size: 1503 360
MscI TGG'CCA
Cuts at: 0 52 208 1330 1590 1846 1863
Size: 52 156 1122 260 256 17
Fragments arranged by size:
1122 260 256 156 52 17
MslI CAynn'nnrTG
Cuts at: 0 268 553 1139 1729 1852 1863
Size: 268 285 586 590 123 11
Fragments arranged by size:
590 586 285 268 123 11
MspA1I CmG'CkG
Cuts at: 0 408 497 600 621 694 1863
Size: 408 89 103 21 73 1169
Fragments arranged by size:
1169 408 103 89 73 21
NaeI GCC'GGC
Cuts at: 0 1650 1863
Size: 1650 213
NcoI C'CATG_G
Cuts at: 0 173 506 1031 1863
Size: 173 333 525 832
Fragments arranged by size:
832 525 333 173
NgoMIV G'CCGG_C
Cuts at: 0 1648 1863
Size: 1648 215
NruI TCG'CGA
Cuts at: 0 376 1863
Size: 376 1487
NspI r_CATG'y
Cuts at: 0 552 1728 1863
Size: 552 1176 135
Fragments arranged by size:
1176 552 135
PasI CC'CwG_GG
Cuts at: 0 540 1863
Size: 540 1323
PflMI CCAn_nnn'nTGG
Cuts at: 0 408 540 1863
Size: 408 132 1323
Fragments arranged by size:
1323 408 132
PfoI T'CCnGG_A
Cuts at: 0 863 1040 1241 1673 1829 1863
Size: 863 177 201 432 156 34
Fragments arranged by size:
863 432 201 177 156 34
PpuMI rG'GwC_Cy
Cuts at: 0 654 1536 1863
Size: 654 882 327
Fragments arranged by size:
882 654 327
PspOMI G'GGCC_C
Cuts at: 0 236 1863
Size: 236 1627
PvuI CG_AT'CG
Cuts at: 0 269 380 1863
Size: 269 111 1483
Fragments arranged by size:
1483 269 111
PvuII CAG'CTG
Cuts at: 0 408 621 1863
Size: 408 213 1242
Fragments arranged by size:
1242 408 213
RsrII CG'GwC_CG
Cuts at: 0 1489 1863
Size: 1489 374
SacII CC_GC'GG
Cuts at: 0 695 1863
Size: 695 1168
SanDI GG'GwC_CC
Cuts at: 0 1536 1863
Size: 1536 327
SexAI A'CCwGG_T
Cuts at: 0 1799 1863
Size: 1799 64
SfcI C'TryA_G
Cuts at: 0 321 1863
Size: 321 1542
SphI G_CATG'C
Cuts at: 0 552 1863
Size: 552 1311
StyI C'CwwG_G
Cuts at: 0 173 506 893 1031 1863
Size: 173 333 387 138 832
Fragments arranged by size:
832 387 333 173 138
Tth111I GACn'n_nGTC
Cuts at: 0 247 1600 1863
Size: 247 1353 263
Fragments arranged by size:
1353 263 247
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1038 1863
Size: 1038 825
XmnI GAAnn'nnTTC
Cuts at: 0 607 925 1863
Size: 607 318 938
Fragments arranged by size:
938 607 318
Enzymes that do cut:
AarI AccI AcuI AfeI AleI AloI AlwNI ApaI
ApaLI ApoI AvaI BamHI BanII BbsI BbvCI BglI
BglII BlpI Bme1580I BmgBI BpmI Bpu10I BsaI BsaHI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspMI BsrBI BsrFI BssHII BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FseI HaeII
Hin4I MmeI MscI MslI MspA1I NaeI NcoI NgoMIV
NruI NspI PasI PflMI PfoI PpuMI PspOMI PvuI
PvuII RsrII SacII SanDI SexAI SfcI SphI StyI
Tth111I XcmI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AflII AflIII AgeI AhdI AlfI
AscI AseI AsiSI AvrII BaeI BanI BbeI BcgI
BciVI BclI BfrBI BmrI BmtI BplI BpuEI BsaAI
BsaBI BsaWI BsiWI BsmI BspEI BspHI BsrDI BsrGI
BssSI BstEII BstZ17I Bsu36I ClaI CspCI EcoICRI EcoRV
FalI FspI FspAI HincII HindIII HpaI KasI KpnI
MfeI MluI NarI NdeI NheI NotI NsiI PacI
PciI PmeI PmlI PpiI PshAI PsiI PspXI PsrI
PstI SacI SalI SapI SbfI ScaI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SrfI SspI
StuI SwaI TaqII TatI TstI XbaI XhoI XmaI
ZraI