Restriction map for
pFN21AE2028
(Linear) (Six-base) MAPSORT of: KIEE2028.seq Check: 6931 from: 1 to: 1833
KIEE2028.orf
With 182 enzymes: SgfI *
July 15, 2010 18:53 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 71 77 669 1833
Size: 71 6 592 1164
Fragments arranged by size:
1164 592 71 6
AccI GT'mk_AC
Cuts at: 0 83 1833
Size: 83 1750
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1124 1495 1833
Size: 1124 371 338
Fragments arranged by size:
1124 371 338
AflII C'TTAA_G
Cuts at: 0 1413 1833
Size: 1413 420
AhdI GACnn_n'nnGTC
Cuts at: 0 211 1833
Size: 211 1622
ApoI r'AATT_y
Cuts at: 0 311 571 1053 1452 1833
Size: 311 260 482 399 381
Fragments arranged by size:
482 399 381 311 260
AvaI C'yCGr_G
Cuts at: 0 4 1833
Size: 4 1829
AvrII C'CTAG_G
Cuts at: 0 1212 1833
Size: 1212 621
BanI G'GyrC_C
Cuts at: 0 1530 1833
Size: 1530 303
BbsI GAAGACnn'nnnn_
Cuts at: 0 554 1833
Size: 554 1279
BciVI GTATCCnnnnn_n'
Cuts at: 0 9 159 1833
Size: 9 150 1674
Fragments arranged by size:
1674 150 9
BclI T'GATC_A
Cuts at: 0 197 1746 1833
Size: 197 1549 87
Fragments arranged by size:
1549 197 87
BfrBI ATG'CAT
Cuts at: 0 1788 1833
Size: 1788 45
BglI GCCn_nnn'nGGC
Cuts at: 0 37 1833
Size: 37 1796
BglII A'GATC_T
Cuts at: 0 738 1833
Size: 738 1095
Bme1580I G_kGCm'C
Cuts at: 0 1533 1550 1833
Size: 1533 17 283
Fragments arranged by size:
1533 283 17
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1550 1833
Size: 1550 283
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 331 1507 1833
Size: 331 1176 326
Fragments arranged by size:
1176 331 326
BsaI GGTCTCn'nnnn_
Cuts at: 0 316 1833
Size: 316 1517
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1505 1535 1833
Size: 1505 30 298
Fragments arranged by size:
1505 298 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 1833
Size: 49 1784
BsiHKAI G_wGCw'C
Cuts at: 0 1775 1833
Size: 1775 58
BsmI GAATG_Cn'
Cuts at: 0 1341 1565 1628 1833
Size: 1341 224 63 205
Fragments arranged by size:
1341 224 205 63
Bsp1286I G_dGCh'C
Cuts at: 0 1533 1550 1775 1833
Size: 1533 17 225 58
Fragments arranged by size:
1533 225 58 17
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 71 77 669 1833
Size: 71 6 592 1164
Fragments arranged by size:
1164 592 71 6
BsrDI GCAATG_nn'
Cuts at: 0 878 1808 1833
Size: 878 930 25
Fragments arranged by size:
930 878 25
BsrGI T'GTAC_A
Cuts at: 0 1571 1833
Size: 1571 262
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1098 1833
Size: 1098 735
BstYI r'GATC_y
Cuts at: 0 738 1479 1719 1833
Size: 738 741 240 114
Fragments arranged by size:
741 738 240 114
BtsI GCAGTG_nn'
Cuts at: 0 417 1833
Size: 417 1416
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1621 1656 1790 1825 1833
Size: 1621 35 134 35 8
Fragments arranged by size:
1621 134 35 35 8
DraI TTT'AAA
Cuts at: 0 1299 1451 1833
Size: 1299 152 382
Fragments arranged by size:
1299 382 152
DrdI GACnn_nn'nnGTC
Cuts at: 0 778 1833
Size: 778 1055
EarI CTCTTCn'nnn_
Cuts at: 0 275 1491 1541 1833
Size: 275 1216 50 292
Fragments arranged by size:
1216 292 275 50
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 331 1124 1495 1507 1833
Size: 331 793 371 12 326
Fragments arranged by size:
793 371 331 326 12
EcoO109I rG'GnC_Cy
Cuts at: 0 174 1833
Size: 174 1659
EcoRI G'AATT_C
Cuts at: 0 1053 1833
Size: 1053 780
FspI TGC'GCA
Cuts at: 0 151 1833
Size: 151 1682
HaeII r_GCGC'y
Cuts at: 0 1666 1833
Size: 1666 167
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 102 134 197 229 276 308 700 732
Size: 102 32 63 32 47 32 392 32
Cuts at: 732 897 929 1833
Size: 165 32 904
Fragments arranged by size:
904 392 165 102 63 47 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 84 1833
Size: 84 1749
HindIII A'AGCT_T
Cuts at: 0 1226 1833
Size: 1226 607
MfeI C'AATT_G
Cuts at: 0 1495 1833
Size: 1495 338
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 600 1833
Size: 600 1233
MslI CAynn'nnrTG
Cuts at: 0 1022 1833
Size: 1022 811
NsiI A_TGCA'T
Cuts at: 0 1790 1833
Size: 1790 43
NspI r_CATG'y
Cuts at: 0 1788 1833
Size: 1788 45
PfoI T'CCnGG_A
Cuts at: 0 1353 1833
Size: 1353 480
PpuMI rG'GwC_Cy
Cuts at: 0 174 1833
Size: 174 1659
PstI C_TGCA'G
Cuts at: 0 79 424 477 1833
Size: 79 345 53 1356
Fragments arranged by size:
1356 345 79 53
SalI G'TCGA_C
Cuts at: 0 82 1833
Size: 82 1751
SfcI C'TryA_G
Cuts at: 0 75 420 473 1048 1833
Size: 75 345 53 575 785
Fragments arranged by size:
785 575 345 75 53
SmlI C'TyrA_G
Cuts at: 0 1413 1833
Size: 1413 420
SphI G_CATG'C
Cuts at: 0 1788 1833
Size: 1788 45
StyI C'CwwG_G
Cuts at: 0 1212 1833
Size: 1212 621
SwaI ATTT'AAAT
Cuts at: 0 1451 1833
Size: 1451 382
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 401 1833
Size: 401 1432
TatI w'GTAC_w
Cuts at: 0 968 1571 1833
Size: 968 603 262
Fragments arranged by size:
968 603 262
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 779 811 950 982 1505 1537 1833
Size: 779 32 139 32 523 32 296
Fragments arranged by size:
779 523 296 139 32 32 32
XmnI GAAnn'nnTTC
Cuts at: 0 1279 1833
Size: 1279 554
Enzymes that do cut:
AarI AccI AcuI AflII AhdI ApoI AvaI AvrII
BanI BbsI BciVI BclI BfrBI BglI BglII Bme1580I
BplI BpmI BsaI BsaXI BsgI BsiHKAI BsmI Bsp1286I
BspMI BsrDI BsrGI BstXI BstYI BtsI CspCI DraI
DrdI EarI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII MfeI MmeI MslI NsiI NspI PfoI
PpuMI PstI SalI SfcI SmlI SphI StyI SwaI
TaqII TatI TstI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflIII AgeI AleI AlfI
AloI AlwNI ApaI ApaLI AscI AseI AsiSI BaeI
BamHI BanII BbeI BbvCI BcgI BlpI BmgBI BmrI
BmtI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsiEI BsiWI BsmBI BspEI BspHI BsrBI BsrFI
BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgI
BtgZI ClaI DraIII EaeI EagI EciI EcoICRI EcoNI
EcoRV FalI FseI FspAI HpaI KasI KpnI MluI
MscI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI PacI PasI PciI PflMI PmeI PmlI
PpiI PshAI PsiI PspOMI PspXI PsrI PvuI PvuII
RsrII SacI SacII SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI
SspI StuI Tth111I XbaI XcmI XhoI XmaI ZraI