Restriction map for
pFN21AE2026
(Linear) (Six-base) MAPSORT of: KIEE2026.seq Check: 4005 from: 1 to: 1827
KIEE2026.orf
With 182 enzymes: SgfI *
July 15, 2010 18:53 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 204 384 1827
Size: 204 180 1443
Fragments arranged by size:
1443 204 180
AatII G_ACGT'C
Cuts at: 0 1319 1827
Size: 1319 508
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 328 1707 1827
Size: 328 1379 120
Fragments arranged by size:
1379 328 120
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1534 1827
Size: 1534 293
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1285 1317 1827
Size: 1285 32 510
Fragments arranged by size:
1285 510 32
AlwNI CAG_nnn'CTG
Cuts at: 0 242 1827
Size: 242 1585
ApaI G_GGCC'C
Cuts at: 0 663 1656 1827
Size: 663 993 171
Fragments arranged by size:
993 663 171
ApaLI G'TGCA_C
Cuts at: 0 916 1827
Size: 916 911
ApoI r'AATT_y
Cuts at: 0 1348 1827
Size: 1348 479
BanI G'GyrC_C
Cuts at: 0 7 1827
Size: 7 1820
BanII G_rGCy'C
Cuts at: 0 464 663 1656 1827
Size: 464 199 993 171
Fragments arranged by size:
993 464 199 171
BbvCI CC'TCA_GC
Cuts at: 0 634 1381 1827
Size: 634 747 446
Fragments arranged by size:
747 634 446
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 336 370 1470 1504 1827
Size: 336 34 1100 34 323
Fragments arranged by size:
1100 336 323 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 960 1827
Size: 960 867
BlpI GC'TnA_GC
Cuts at: 0 290 1081 1827
Size: 290 791 746
Fragments arranged by size:
791 746 290
Bme1580I G_kGCm'C
Cuts at: 0 663 920 1656 1827
Size: 663 257 736 171
Fragments arranged by size:
736 663 257 171
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 519 1176 1248 1530 1827
Size: 519 657 72 282 297
Fragments arranged by size:
657 519 297 282 72
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 478 598 1042 1240 1443 1690 1743 1751
Size: 478 120 444 198 203 247 53 8
Cuts at: 1751 1827
Size: 76
Fragments arranged by size:
478 444 247 203 198 120 76 53
8
Bpu10I CC'TnA_GC
Cuts at: 0 634 748 1381 1827
Size: 634 114 633 446
Fragments arranged by size:
634 633 446 114
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 757 907 1827
Size: 757 150 920
Fragments arranged by size:
920 757 150
BsaI GGTCTCn'nnnn_
Cuts at: 0 772 796 829 1827
Size: 772 24 33 998
Fragments arranged by size:
998 772 33 24
BsaAI yAC'GTr
Cuts at: 0 687 1827
Size: 687 1140
BsaBI GATnn'nnATC
Cuts at: 0 1109 1827
Size: 1109 718
BsaHI Gr'CG_yC
Cuts at: 0 870 1316 1827
Size: 870 446 511
Fragments arranged by size:
870 511 446
BsaWI w'CCGG_w
Cuts at: 0 1304 1827
Size: 1304 523
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 127 157 232 262 1285 1315 1827
Size: 127 30 75 30 1023 30 512
Fragments arranged by size:
1023 512 127 75 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 55 135 1039 1237 1447 1795 1798 1827
Size: 55 80 904 198 210 348 3 29
Fragments arranged by size:
904 348 210 198 80 55 29 3
BseYI C'CCAG_C
Cuts at: 0 258 969 1827
Size: 258 711 858
Fragments arranged by size:
858 711 258
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 15 540 640 1664 1827
Size: 15 525 100 1024 163
Fragments arranged by size:
1024 525 163 100 15
BsiHKAI G_wGCw'C
Cuts at: 0 920 1277 1827
Size: 920 357 550
Fragments arranged by size:
920 550 357
BsmBI CGTCTCn'nnnn_
Cuts at: 0 612 1827
Size: 612 1215
Bsp1286I G_dGCh'C
Cuts at: 0 464 663 920 1277 1656 1827
Size: 464 199 257 357 379 171
Fragments arranged by size:
464 379 357 257 199 171
BspEI T'CCGG_A
Cuts at: 0 1304 1827
Size: 1304 523
BspHI T'CATG_A
Cuts at: 0 119 626 1827
Size: 119 507 1201
Fragments arranged by size:
1201 507 119
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 204 384 1085 1827
Size: 204 180 701 742
Fragments arranged by size:
742 701 204 180
BsrBI CCG'CTC
Cuts at: 0 582 1827
Size: 582 1245
BsrFI r'CCGG_y
Cuts at: 0 866 1827
Size: 866 961
BssSI C'ACGA_G
Cuts at: 0 1185 1264 1827
Size: 1185 79 563
Fragments arranged by size:
1185 563 79
BstAPI GCAn_nnn'nTGC
Cuts at: 0 105 1827
Size: 105 1722
BstEII G'GTnAC_C
Cuts at: 0 1038 1827
Size: 1038 789
BstXI CCAn_nnnn'nTGG
Cuts at: 0 471 1827
Size: 471 1356
BstYI r'GATC_y
Cuts at: 0 245 515 1772 1827
Size: 245 270 1257 55
Fragments arranged by size:
1257 270 245 55
BtgI C'CryG_G
Cuts at: 0 51 464 1034 1362 1827
Size: 51 413 570 328 465
Fragments arranged by size:
570 465 413 328 51
BtsI GCAGTG_nn'
Cuts at: 0 740 949 1539 1621 1827
Size: 740 209 590 82 206
Fragments arranged by size:
740 590 209 206 82
DrdI GACnn_nn'nnGTC
Cuts at: 0 571 1827
Size: 571 1256
EaeI y'GGCC_r
Cuts at: 0 158 1031 1051 1329 1827
Size: 158 873 20 278 498
Fragments arranged by size:
873 498 278 158 20
EarI CTCTTCn'nnn_
Cuts at: 0 601 1827
Size: 601 1226
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 240 490 706 1827
Size: 240 250 216 1121
Fragments arranged by size:
1121 250 240 216
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 328 478 598 1042 1240 1443 1690 1707
Size: 328 150 120 444 198 203 247 17
Cuts at: 1707 1743 1751 1827
Size: 36 8 76
Fragments arranged by size:
444 328 247 203 198 150 120 76
36 17 8
EcoNI CCTnn'n_nnAGG
Cuts at: 0 22 523 1827
Size: 22 501 1304
Fragments arranged by size:
1304 501 22
EcoO109I rG'GnC_Cy
Cuts at: 0 93 660 744 927 1260 1395 1575 1652
Size: 93 567 84 183 333 135 180 77
Cuts at: 1652 1827
Size: 175
Fragments arranged by size:
567 333 183 180 175 135 93 84
77
EcoRI G'AATT_C
Cuts at: 0 1348 1827
Size: 1348 479
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1026 1827
Size: 1026 801
FspI TGC'GCA
Cuts at: 0 109 1827
Size: 109 1718
FspAI rTGC'GCAy
Cuts at: 0 109 1827
Size: 109 1718
HaeII r_GCGC'y
Cuts at: 0 1388 1827
Size: 1388 439
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1615 1647 1827
Size: 1615 32 180
Fragments arranged by size:
1615 180 32
MscI TGG'CCA
Cuts at: 0 1033 1331 1827
Size: 1033 298 496
Fragments arranged by size:
1033 496 298
MslI CAynn'nnrTG
Cuts at: 0 727 1827
Size: 727 1100
MspA1I CmG'CkG
Cuts at: 0 53 102 153 262 405 657 708 945
Size: 53 49 51 109 143 252 51 237
Cuts at: 945 1137 1149 1827
Size: 192 12 678
Fragments arranged by size:
678 252 237 192 143 109 53 51
51 49 12
NcoI C'CATG_G
Cuts at: 0 464 1034 1827
Size: 464 570 793
Fragments arranged by size:
793 570 464
NspI r_CATG'y
Cuts at: 0 114 1827
Size: 114 1713
PfoI T'CCnGG_A
Cuts at: 0 79 187 575 1019 1502 1827
Size: 79 108 388 444 483 325
Fragments arranged by size:
483 444 388 325 108 79
PmlI CAC'GTG
Cuts at: 0 687 1827
Size: 687 1140
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1285 1317 1827
Size: 1285 32 510
Fragments arranged by size:
1285 510 32
PpuMI rG'GwC_Cy
Cuts at: 0 93 927 1395 1827
Size: 93 834 468 432
Fragments arranged by size:
834 468 432 93
PspOMI G'GGCC_C
Cuts at: 0 659 1652 1827
Size: 659 993 175
Fragments arranged by size:
993 659 175
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1360 1392 1827
Size: 1360 32 435
Fragments arranged by size:
1360 435 32
PstI C_TGCA'G
Cuts at: 0 215 713 944 1148 1827
Size: 215 498 231 204 679
Fragments arranged by size:
679 498 231 215 204
PvuII CAG'CTG
Cuts at: 0 102 153 708 945 1137 1827
Size: 102 51 555 237 192 690
Fragments arranged by size:
690 555 237 192 102 51
SacII CC_GC'GG
Cuts at: 0 54 1827
Size: 54 1773
SanDI GG'GwC_CC
Cuts at: 0 93 1827
Size: 93 1734
SbfI CC_TGCA'GG
Cuts at: 0 215 1827
Size: 215 1612
SexAI A'CCwGG_T
Cuts at: 0 230 929 1827
Size: 230 699 898
Fragments arranged by size:
898 699 230
SfcI C'TryA_G
Cuts at: 0 211 703 709 940 1144 1827
Size: 211 492 6 231 204 683
Fragments arranged by size:
683 492 231 211 204 6
SmlI C'TyrA_G
Cuts at: 0 772 922 1827
Size: 772 150 905
Fragments arranged by size:
905 772 150
SpeI A'CTAG_T
Cuts at: 0 834 1827
Size: 834 993
StuI AGG'CCT
Cuts at: 0 1764 1827
Size: 1764 63
StyI C'CwwG_G
Cuts at: 0 464 1034 1827
Size: 464 570 793
Fragments arranged by size:
793 570 464
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 606 901 1710 1827
Size: 606 295 809 117
Fragments arranged by size:
809 606 295 117
TatI w'GTAC_w
Cuts at: 0 1059 1827
Size: 1059 768
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 665 697 1827
Size: 665 32 1130
Fragments arranged by size:
1130 665 32
Tth111I GACn'n_nGTC
Cuts at: 0 367 1827
Size: 367 1460
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1248 1360 1411 1467 1827
Size: 1248 112 51 56 360
Fragments arranged by size:
1248 360 112 56 51
ZraI GAC'GTC
Cuts at: 0 1317 1827
Size: 1317 510
Enzymes that do cut:
AarI AatII AcuI AlfI AloI AlwNI ApaI ApaLI
ApoI BanI BanII BbvCI BcgI BglI BlpI Bme1580I
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrFI BssSI BstAPI BstEII
BstXI BstYI BtgI BtsI DrdI EaeI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII
Hin4I MscI MslI MspA1I NcoI NspI PfoI PmlI
PpiI PpuMI PspOMI PsrI PstI PvuII SacII SanDI
SbfI SexAI SfcI SmlI SpeI StuI StyI TaqII
TatI TstI Tth111I XcmI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AscI AseI AsiSI AvaI AvrII BaeI BamHI
BbeI BbsI BciVI BclI BfrBI BglII BmgBI BmrI
BmtI BsiEI BsiWI BsmI BsrDI BsrGI BssHII BstBI
BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII EagI
EcoICRI EcoRV FseI HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI PacI PasI PciI PflMI PmeI
PshAI PsiI PspXI PvuI RsrII SacI SalI SapI
ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI SphI
SrfI SspI SwaI XbaI XhoI XmaI XmnI