Restriction map for
pFN21AE2023
(Linear) (Six-base) MAPSORT of: KIEE2023.seq Check: 2924 from: 1 to: 1797
KIEE2023.orf
With 182 enzymes: SgfI *
July 15, 2010 18:53 ..
AccI GT'mk_AC
Cuts at: 0 1669 1797
Size: 1669 128
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1470 1567 1797
Size: 1470 97 230
Fragments arranged by size:
1470 230 97
AfeI AGC'GCT
Cuts at: 0 772 991 1797
Size: 772 219 806
Fragments arranged by size:
806 772 219
AflIII A'CryG_T
Cuts at: 0 642 1797
Size: 642 1155
AhdI GACnn_n'nnGTC
Cuts at: 0 412 1797
Size: 412 1385
AleI CACnn'nnGTG
Cuts at: 0 1487 1790 1797
Size: 1487 303 7
Fragments arranged by size:
1487 303 7
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1772 1797
Size: 1772 25
AlwNI CAG_nnn'CTG
Cuts at: 0 213 1272 1612 1737 1752 1797
Size: 213 1059 340 125 15 45
Fragments arranged by size:
1059 340 213 125 45 15
ApaI G_GGCC'C
Cuts at: 0 857 1149 1250 1387 1797
Size: 857 292 101 137 410
Fragments arranged by size:
857 410 292 137 101
ApoI r'AATT_y
Cuts at: 0 61 1797
Size: 61 1736
AvaI C'yCGr_G
Cuts at: 0 1311 1391 1532 1797
Size: 1311 80 141 265
Fragments arranged by size:
1311 265 141 80
BanI G'GyrC_C
Cuts at: 0 88 340 1797
Size: 88 252 1457
Fragments arranged by size:
1457 252 88
BanII G_rGCy'C
Cuts at: 0 857 1010 1044 1149 1250 1387 1398 1433
Size: 857 153 34 105 101 137 11 35
Cuts at: 1433 1592 1606 1624 1797
Size: 159 14 18 173
Fragments arranged by size:
857 173 159 153 137 105 101 35
34 18 14 11
BbsI GAAGACnn'nnnn_
Cuts at: 0 453 1797
Size: 453 1344
BbvCI CC'TCA_GC
Cuts at: 0 119 311 1797
Size: 119 192 1486
Fragments arranged by size:
1486 192 119
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1194 1228 1797
Size: 1194 34 569
Fragments arranged by size:
1194 569 34
BglI GCCn_nnn'nGGC
Cuts at: 0 86 1628 1797
Size: 86 1542 169
Fragments arranged by size:
1542 169 86
Bme1580I G_kGCm'C
Cuts at: 0 91 575 857 1149 1250 1387 1797
Size: 91 484 282 292 101 137 410
Fragments arranged by size:
484 410 292 282 137 101 91
BmgBI CAC'GTC
Cuts at: 0 570 1797
Size: 570 1227
BmrI ACTGGGnnnn_n'
Cuts at: 0 850 1080 1588 1784 1797
Size: 850 230 508 196 13
Fragments arranged by size:
850 508 230 196 13
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 144 480 1785 1797
Size: 144 336 1305 12
Fragments arranged by size:
1305 336 144 12
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 382 523 564 625 1348 1797
Size: 382 141 41 61 723 449
Fragments arranged by size:
723 449 382 141 61 41
Bpu10I CC'TnA_GC
Cuts at: 0 119 311 1797
Size: 119 192 1486
Fragments arranged by size:
1486 192 119
BsaI GGTCTCn'nnnn_
Cuts at: 0 399 1797
Size: 399 1398
BsaHI Gr'CG_yC
Cuts at: 0 218 1797
Size: 218 1579
BsaWI w'CCGG_w
Cuts at: 0 164 297 1797
Size: 164 133 1500
Fragments arranged by size:
1500 164 133
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 136 796 1015 1376 1379 1797
Size: 136 660 219 361 3 418
Fragments arranged by size:
660 418 361 219 136 3
BseYI C'CCAG_C
Cuts at: 0 171 213 243 856 986 1140 1434 1745
Size: 171 42 30 613 130 154 294 311
Cuts at: 1745 1781 1797
Size: 36 16
Fragments arranged by size:
613 311 294 171 154 130 42 36
30 16
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1140 1618 1797
Size: 1140 478 179
Fragments arranged by size:
1140 478 179
BsiHKAI G_wGCw'C
Cuts at: 0 902 1010 1433 1797
Size: 902 108 423 364
Fragments arranged by size:
902 423 364 108
BsiWI C'GTAC_G
Cuts at: 0 1286 1797
Size: 1286 511
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1296 1797
Size: 1296 501
Bsp1286I G_dGCh'C
Cuts at: 0 91 575 857 902 1010 1044 1149 1250
Size: 91 484 282 45 108 34 105 101
Cuts at: 1250 1387 1398 1433 1592 1606 1624 1797
Size: 137 11 35 159 14 18 173
Fragments arranged by size:
484 282 173 159 137 108 105 101
91 45 35 34 18 14 11
BspEI T'CCGG_A
Cuts at: 0 164 1797
Size: 164 1633
BsrBI CCG'CTC
Cuts at: 0 279 507 756 786 972 1797
Size: 279 228 249 30 186 825
Fragments arranged by size:
825 279 249 228 186 30
BsrFI r'CCGG_y
Cuts at: 0 845 1064 1797
Size: 845 219 733
Fragments arranged by size:
845 733 219
BstAPI GCAn_nnn'nTGC
Cuts at: 0 594 1797
Size: 594 1203
BstYI r'GATC_y
Cuts at: 0 191 617 1423 1763 1797
Size: 191 426 806 340 34
Fragments arranged by size:
806 426 340 191 34
BstZ17I GTA'TAC
Cuts at: 0 1670 1797
Size: 1670 127
Bsu36I CC'TnA_GG
Cuts at: 0 347 1797
Size: 347 1450
BtgI C'CryG_G
Cuts at: 0 185 328 444 1469 1624 1797
Size: 185 143 116 1025 155 173
Fragments arranged by size:
1025 185 173 155 143 116
BtsI GCAGTG_nn'
Cuts at: 0 494 1123 1236 1558 1797
Size: 494 629 113 322 239
Fragments arranged by size:
629 494 322 239 113
ClaI AT'CG_AT
Cuts at: 0 419 1797
Size: 419 1378
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 875 910 1797
Size: 875 35 887
Fragments arranged by size:
887 875 35
DraIII CAC_nnn'GTG
Cuts at: 0 1704 1797
Size: 1704 93
EaeI y'GGCC_r
Cuts at: 0 113 434 973 1062 1461 1479 1646 1797
Size: 113 321 539 89 399 18 167 151
Fragments arranged by size:
539 399 321 167 151 113 89 18
EarI CTCTTCn'nnn_
Cuts at: 0 374 1797
Size: 374 1423
EcoICRI GAG'CTC
Cuts at: 0 1008 1431 1797
Size: 1008 423 366
Fragments arranged by size:
1008 423 366
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 382 523 564 625 1348 1470 1567 1797
Size: 382 141 41 61 723 122 97 230
Fragments arranged by size:
723 382 230 141 122 97 61 41
EcoNI CCTnn'n_nnAGG
Cuts at: 0 924 1648 1705 1797
Size: 924 724 57 92
Fragments arranged by size:
924 724 92 57
EcoO109I rG'GnC_Cy
Cuts at: 0 1145 1163 1369 1797
Size: 1145 18 206 428
Fragments arranged by size:
1145 428 206 18
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 174 477 1797
Size: 174 303 1320
Fragments arranged by size:
1320 303 174
HaeII r_GCGC'y
Cuts at: 0 288 774 993 1725 1797
Size: 288 486 219 732 72
Fragments arranged by size:
732 486 288 219 72
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 398 430 1415 1447 1528 1560 1797
Size: 398 32 985 32 81 32 237
Fragments arranged by size:
985 398 237 81 32 32 32
MluI A'CGCG_T
Cuts at: 0 642 1797
Size: 642 1155
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 749 1322 1797
Size: 749 573 475
Fragments arranged by size:
749 573 475
MscI TGG'CCA
Cuts at: 0 436 1481 1648 1797
Size: 436 1045 167 149
Fragments arranged by size:
1045 436 167 149
MslI CAynn'nnrTG
Cuts at: 0 526 1487 1790 1797
Size: 526 961 303 7
Fragments arranged by size:
961 526 303 7
MspA1I CmG'CkG
Cuts at: 0 118 330 1752 1797
Size: 118 212 1422 45
Fragments arranged by size:
1422 212 118 45
NaeI GCC'GGC
Cuts at: 0 847 1066 1797
Size: 847 219 731
Fragments arranged by size:
847 731 219
NcoI C'CATG_G
Cuts at: 0 444 1469 1797
Size: 444 1025 328
Fragments arranged by size:
1025 444 328
NgoMIV G'CCGG_C
Cuts at: 0 845 1064 1797
Size: 845 219 733
Fragments arranged by size:
845 733 219
PasI CC'CwG_GG
Cuts at: 0 1159 1606 1797
Size: 1159 447 191
Fragments arranged by size:
1159 447 191
PflMI CCAn_nnn'nTGG
Cuts at: 0 1256 1612 1752 1797
Size: 1256 356 140 45
Fragments arranged by size:
1256 356 140 45
PfoI T'CCnGG_A
Cuts at: 0 29 541 1736 1797
Size: 29 512 1195 61
Fragments arranged by size:
1195 512 61 29
PpuMI rG'GwC_Cy
Cuts at: 0 1163 1797
Size: 1163 634
PspOMI G'GGCC_C
Cuts at: 0 853 1145 1246 1383 1797
Size: 853 292 101 137 414
Fragments arranged by size:
853 414 292 137 101
PstI C_TGCA'G
Cuts at: 0 146 209 1797
Size: 146 63 1588
Fragments arranged by size:
1588 146 63
PvuII CAG'CTG
Cuts at: 0 1752 1797
Size: 1752 45
SacI G_AGCT'C
Cuts at: 0 1010 1433 1797
Size: 1010 423 364
Fragments arranged by size:
1010 423 364
SacII CC_GC'GG
Cuts at: 0 331 1797
Size: 331 1466
SanDI GG'GwC_CC
Cuts at: 0 1163 1797
Size: 1163 634
ScaI AGT'ACT
Cuts at: 0 1540 1797
Size: 1540 257
SexAI A'CCwGG_T
Cuts at: 0 1700 1797
Size: 1700 97
SfcI C'TryA_G
Cuts at: 0 142 205 1410 1797
Size: 142 63 1205 387
Fragments arranged by size:
1205 387 142 63
SmlI C'TyrA_G
Cuts at: 0 1532 1797
Size: 1532 265
StuI AGG'CCT
Cuts at: 0 127 1797
Size: 127 1670
StyI C'CwwG_G
Cuts at: 0 94 444 747 1469 1649 1797
Size: 94 350 303 722 180 148
Fragments arranged by size:
722 350 303 180 148 94
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 235 637 980 1462 1584 1797
Size: 235 402 343 482 122 213
Fragments arranged by size:
482 402 343 235 213 122
TatI w'GTAC_w
Cuts at: 0 797 1538 1797
Size: 797 741 259
Fragments arranged by size:
797 741 259
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 240 1797
Size: 240 1557
XhoI C'TCGA_G
Cuts at: 0 1532 1797
Size: 1532 265
XmnI GAAnn'nnTTC
Cuts at: 0 772 1797
Size: 772 1025
Enzymes that do cut:
AccI AcuI AfeI AflIII AhdI AleI AlfI AlwNI
ApaI ApoI AvaI BanI BanII BbsI BbvCI BcgI
BglI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BsaI
BsaHI BsaWI BseRI BseYI BsgI BsiHKAI BsiWI BsmBI
Bsp1286I BspEI BsrBI BsrFI BstAPI BstYI BstZ17I Bsu36I
BtgI BtsI ClaI CspCI DraIII EaeI EarI EcoICRI
Eco57MI EcoNI EcoO109I FalI HaeII Hin4I MluI MmeI
MscI MslI MspA1I NaeI NcoI NgoMIV PasI PflMI
PfoI PpuMI PspOMI PstI PvuII SacI SacII SanDI
ScaI SexAI SfcI SmlI StuI StyI TaqII TatI
XcmI XhoI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AgeI AloI ApaLI
AscI AseI AsiSI AvrII BaeI BamHI BbeI BciVI
BclI BfrBI BglII BlpI BmtI BpuEI BsaAI BsaBI
BsaXI BsiEI BsmI BspHI BspMI BsrDI BsrGI BssHII
BssSI BstBI BstEII BstXI BtgZI DraI DrdI EagI
EciI EcoRI EcoRV FseI FspI FspAI HincII HindIII
HpaI KasI KpnI MfeI NarI NdeI NheI NotI
NruI NsiI NspI PacI PciI PmeI PmlI PpiI
PshAI PsiI PspXI PsrI PvuI RsrII SalI SapI
SbfI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI SwaI TstI Tth111I XbaI XmaI
ZraI