Restriction map for
pFN21AE2014
(Linear) (Six-base) MAPSORT of: KIEE2014.seq Check: 278 from: 1 to: 1776
KIEE2014.orf
With 182 enzymes: SgfI *
July 15, 2010 18:53 ..
AatII G_ACGT'C
Cuts at: 0 1607 1776
Size: 1607 169
AccI GT'mk_AC
Cuts at: 0 873 1026 1776
Size: 873 153 750
Fragments arranged by size:
873 750 153
AclI AA'CG_TT
Cuts at: 0 841 1776
Size: 841 935
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 54 263 369 746 761 1564 1776
Size: 54 209 106 377 15 803 212
Fragments arranged by size:
803 377 212 209 106 54 15
AfeI AGC'GCT
Cuts at: 0 880 1776
Size: 880 896
AflIII A'CryG_T
Cuts at: 0 895 1061 1776
Size: 895 166 715
Fragments arranged by size:
895 715 166
AhdI GACnn_n'nnGTC
Cuts at: 0 1018 1776
Size: 1018 758
AleI CACnn'nnGTG
Cuts at: 0 1435 1776
Size: 1435 341
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 499 1082 1776
Size: 499 583 694
Fragments arranged by size:
694 583 499
AlwNI CAG_nnn'CTG
Cuts at: 0 505 1776
Size: 505 1271
ApaLI G'TGCA_C
Cuts at: 0 626 789 1416 1776
Size: 626 163 627 360
Fragments arranged by size:
627 626 360 163
AvaI C'yCGr_G
Cuts at: 0 925 1707 1776
Size: 925 782 69
Fragments arranged by size:
925 782 69
BanI G'GyrC_C
Cuts at: 0 748 1776
Size: 748 1028
BanII G_rGCy'C
Cuts at: 0 35 1460 1714 1776
Size: 35 1425 254 62
Fragments arranged by size:
1425 254 62 35
BbeI G_GCGC'C
Cuts at: 0 752 1776
Size: 752 1024
BbsI GAAGACnn'nnnn_
Cuts at: 0 269 1330 1776
Size: 269 1061 446
Fragments arranged by size:
1061 446 269
BbvCI CC'TCA_GC
Cuts at: 0 126 1776
Size: 126 1650
BciVI GTATCCnnnnn_n'
Cuts at: 0 1173 1776
Size: 1173 603
BclI T'GATC_A
Cuts at: 0 215 1776
Size: 215 1561
BfrBI ATG'CAT
Cuts at: 0 329 1776
Size: 329 1447
BglII A'GATC_T
Cuts at: 0 1566 1776
Size: 1566 210
Bme1580I G_kGCm'C
Cuts at: 0 630 793 1420 1776
Size: 630 163 627 356
Fragments arranged by size:
630 627 356 163
BmrI ACTGGGnnnn_n'
Cuts at: 0 754 1275 1776
Size: 754 521 501
Fragments arranged by size:
754 521 501
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 823 1127 1639 1731 1776
Size: 823 304 512 92 45
Fragments arranged by size:
823 512 304 92 45
Bpu10I CC'TnA_GC
Cuts at: 0 126 1776
Size: 126 1650
BsaI GGTCTCn'nnnn_
Cuts at: 0 217 1543 1648 1776
Size: 217 1326 105 128
Fragments arranged by size:
1326 217 128 105
BsaAI yAC'GTr
Cuts at: 0 896 1064 1776
Size: 896 168 712
Fragments arranged by size:
896 712 168
BsaBI GATnn'nnATC
Cuts at: 0 1687 1776
Size: 1687 89
BsaHI Gr'CG_yC
Cuts at: 0 749 1604 1776
Size: 749 855 172
Fragments arranged by size:
855 749 172
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 152 182 1776
Size: 152 30 1594
Fragments arranged by size:
1594 152 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 115 1480 1543 1546 1776
Size: 115 1365 63 3 230
Fragments arranged by size:
1365 230 115 63 3
BseYI C'CCAG_C
Cuts at: 0 881 1776
Size: 881 895
BsiEI CG_ry'CG
Cuts at: 0 83 195 1776
Size: 83 112 1581
Fragments arranged by size:
1581 112 83
BsiHKAI G_wGCw'C
Cuts at: 0 35 630 734 793 1370 1420 1460 1776
Size: 35 595 104 59 577 50 40 316
Fragments arranged by size:
595 577 316 104 59 50 40 35
BsmI GAATG_Cn'
Cuts at: 0 331 1776
Size: 331 1445
Bsp1286I G_dGCh'C
Cuts at: 0 35 630 734 793 1370 1420 1460 1714
Size: 35 595 104 59 577 50 40 254
Cuts at: 1714 1776
Size: 62
Fragments arranged by size:
595 577 254 104 62 59 50 40
35
BspHI T'CATG_A
Cuts at: 0 522 1776
Size: 522 1254
BsrDI GCAATG_nn'
Cuts at: 0 518 669 1776
Size: 518 151 1107
Fragments arranged by size:
1107 518 151
BsrFI r'CCGG_y
Cuts at: 0 58 908 1776
Size: 58 850 868
Fragments arranged by size:
868 850 58
BstYI r'GATC_y
Cuts at: 0 1566 1583 1776
Size: 1566 17 193
Fragments arranged by size:
1566 193 17
BtgI C'CryG_G
Cuts at: 0 77 1694 1776
Size: 77 1617 82
Fragments arranged by size:
1617 82 77
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1674 1776
Size: 1674 102
BtsI GCAGTG_nn'
Cuts at: 0 293 483 1737 1776
Size: 293 190 1254 39
Fragments arranged by size:
1254 293 190 39
DraI TTT'AAA
Cuts at: 0 1335 1776
Size: 1335 441
DraIII CAC_nnn'GTG
Cuts at: 0 797 1776
Size: 797 979
DrdI GACnn_nn'nnGTC
Cuts at: 0 231 1776
Size: 231 1545
EaeI y'GGCC_r
Cuts at: 0 80 192 666 996 1439 1776
Size: 80 112 474 330 443 337
Fragments arranged by size:
474 443 337 330 112 80
EagI C'GGCC_G
Cuts at: 0 80 192 1776
Size: 80 112 1584
Fragments arranged by size:
1584 112 80
EarI CTCTTCn'nnn_
Cuts at: 0 1187 1725 1776
Size: 1187 538 51
Fragments arranged by size:
1187 538 51
EcoICRI GAG'CTC
Cuts at: 0 33 1458 1776
Size: 33 1425 318
Fragments arranged by size:
1425 318 33
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 54 263 369 746 761 823 1127 1564
Size: 54 209 106 377 15 62 304 437
Cuts at: 1564 1639 1731 1776
Size: 75 92 45
Fragments arranged by size:
437 377 304 209 106 92 75 62
54 45 15
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 293 1776
Size: 293 1483
FspI TGC'GCA
Cuts at: 0 299 1776
Size: 299 1477
HaeII r_GCGC'y
Cuts at: 0 77 752 882 1776
Size: 77 675 130 894
Fragments arranged by size:
894 675 130 77
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 17 49 165 197 337 369 1004 1036
Size: 17 32 116 32 140 32 635 32
Cuts at: 1036 1626 1658 1776
Size: 590 32 118
Fragments arranged by size:
635 590 140 118 116 32 32 32
32 32 17
HincII GTy'rAC
Cuts at: 0 1027 1776
Size: 1027 749
KasI G'GCGC_C
Cuts at: 0 748 1776
Size: 748 1028
MfeI C'AATT_G
Cuts at: 0 1343 1776
Size: 1343 433
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 260 1776
Size: 260 1516
MscI TGG'CCA
Cuts at: 0 1441 1776
Size: 1441 335
MslI CAynn'nnrTG
Cuts at: 0 1064 1435 1693 1776
Size: 1064 371 258 83
Fragments arranged by size:
1064 371 258 83
MspA1I CmG'CkG
Cuts at: 0 79 130 1245 1596 1776
Size: 79 51 1115 351 180
Fragments arranged by size:
1115 351 180 79 51
NaeI GCC'GGC
Cuts at: 0 60 1776
Size: 60 1716
NarI GG'CG_CC
Cuts at: 0 749 1776
Size: 749 1027
NcoI C'CATG_G
Cuts at: 0 1694 1776
Size: 1694 82
NgoMIV G'CCGG_C
Cuts at: 0 58 1776
Size: 58 1718
NsiI A_TGCA'T
Cuts at: 0 331 1776
Size: 331 1445
NspI r_CATG'y
Cuts at: 0 662 1033 1776
Size: 662 371 743
Fragments arranged by size:
743 662 371
PflMI CCAn_nnn'nTGG
Cuts at: 0 606 1776
Size: 606 1170
PfoI T'CCnGG_A
Cuts at: 0 1586 1670 1747 1776
Size: 1586 84 77 29
Fragments arranged by size:
1586 84 77 29
PmlI CAC'GTG
Cuts at: 0 896 1776
Size: 896 880
PsiI TTA'TAA
Cuts at: 0 1037 1776
Size: 1037 739
PspXI vC'TCGA_Gb
Cuts at: 0 1707 1776
Size: 1707 69
PstI C_TGCA'G
Cuts at: 0 1514 1574 1776
Size: 1514 60 202
Fragments arranged by size:
1514 202 60
PvuII CAG'CTG
Cuts at: 0 130 1245 1596 1776
Size: 130 1115 351 180
Fragments arranged by size:
1115 351 180 130
SacI G_AGCT'C
Cuts at: 0 35 1460 1776
Size: 35 1425 316
Fragments arranged by size:
1425 316 35
SacII CC_GC'GG
Cuts at: 0 80 1776
Size: 80 1696
SalI G'TCGA_C
Cuts at: 0 1025 1776
Size: 1025 751
SfcI C'TryA_G
Cuts at: 0 131 257 874 1510 1570 1776
Size: 131 126 617 636 60 206
Fragments arranged by size:
636 617 206 131 126 60
SfoI GGC'GCC
Cuts at: 0 750 1776
Size: 750 1026
SmlI C'TyrA_G
Cuts at: 0 1707 1776
Size: 1707 69
StyI C'CwwG_G
Cuts at: 0 1694 1776
Size: 1694 82
TatI w'GTAC_w
Cuts at: 0 1158 1776
Size: 1158 618
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1399 1431 1776
Size: 1399 32 345
Fragments arranged by size:
1399 345 32
XbaI T'CTAG_A
Cuts at: 0 1362 1776
Size: 1362 414
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 603 1006 1776
Size: 603 403 770
Fragments arranged by size:
770 603 403
XhoI C'TCGA_G
Cuts at: 0 1707 1776
Size: 1707 69
XmnI GAAnn'nnTTC
Cuts at: 0 410 1776
Size: 410 1366
ZraI GAC'GTC
Cuts at: 0 1605 1776
Size: 1605 171
Enzymes that do cut:
AatII AccI AclI AcuI AfeI AflIII AhdI AleI
AlfI AlwNI ApaLI AvaI BanI BanII BbeI BbsI
BbvCI BciVI BclI BfrBI BglII Bme1580I BmrI BpmI
Bpu10I BsaI BsaAI BsaBI BsaHI BsaXI BseRI BseYI
BsiEI BsiHKAI BsmI Bsp1286I BspHI BsrDI BsrFI BstYI
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EcoICRI Eco57MI FalI FspI HaeII Hin4I HincII
KasI MfeI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NsiI NspI PflMI PfoI PmlI PsiI
PspXI PstI PvuII SacI SacII SalI SfcI SfoI
SmlI StyI TatI TstI XbaI XcmI XhoI XmnI
ZraI
Enzymes that do not cut:
AarI Acc65I AflII AgeI AloI ApaI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BcgI BglI BlpI
BmgBI BmtI BplI BpuEI BsaWI BsgI BsiWI BsmBI
BspEI BspMI BsrBI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstZ17I Bsu36I ClaI CspCI EciI EcoNI
EcoO109I EcoRI EcoRV FseI FspAI HindIII HpaI KpnI
MluI NdeI NheI NotI NruI PacI PasI PciI
PmeI PpiI PpuMI PshAI PspOMI PsrI PvuI RsrII
SanDI SapI SbfI ScaI SexAI SfiI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI
TaqII Tth111I XmaI