Restriction map for
pFN21AE2007
(Linear) (Six-base) MAPSORT of: KIEE2007.seq Check: 8414 from: 1 to: 1749
KIEE2007.orf
With 182 enzymes: SgfI *
July 15, 2010 18:52 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1050 1749
Size: 1050 699
AccI GT'mk_AC
Cuts at: 0 543 1190 1749
Size: 543 647 559
Fragments arranged by size:
647 559 543
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1435 1749
Size: 1435 314
AflII C'TTAA_G
Cuts at: 0 42 1749
Size: 42 1707
AflIII A'CryG_T
Cuts at: 0 546 1749
Size: 546 1203
AleI CACnn'nnGTG
Cuts at: 0 217 1452 1681 1749
Size: 217 1235 229 68
Fragments arranged by size:
1235 229 217 68
ApoI r'AATT_y
Cuts at: 0 528 914 1100 1749
Size: 528 386 186 649
Fragments arranged by size:
649 528 386 186
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 175 208 1749
Size: 175 33 1541
Fragments arranged by size:
1541 175 33
BanI G'GyrC_C
Cuts at: 0 169 1749
Size: 169 1580
BbsI GAAGACnn'nnnn_
Cuts at: 0 129 271 951 1749
Size: 129 142 680 798
Fragments arranged by size:
798 680 142 129
BciVI GTATCCnnnnn_n'
Cuts at: 0 1190 1749
Size: 1190 559
BfrBI ATG'CAT
Cuts at: 0 161 1400 1749
Size: 161 1239 349
Fragments arranged by size:
1239 349 161
BglI GCCn_nnn'nGGC
Cuts at: 0 454 1749
Size: 454 1295
BlpI GC'TnA_GC
Cuts at: 0 99 1749
Size: 99 1650
Bme1580I G_kGCm'C
Cuts at: 0 172 202 1749
Size: 172 30 1547
Fragments arranged by size:
1547 172 30
BmrI ACTGGGnnnn_n'
Cuts at: 0 1051 1749
Size: 1051 698
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 453 1595 1749
Size: 453 1142 154
Fragments arranged by size:
1142 453 154
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 56 884 1749
Size: 56 828 865
Fragments arranged by size:
865 828 56
BsaI GGTCTCn'nnnn_
Cuts at: 0 562 737 1040 1373 1749
Size: 562 175 303 333 376
Fragments arranged by size:
562 376 333 303 175
BsaAI yAC'GTr
Cuts at: 0 1233 1749
Size: 1233 516
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 79 109 1749
Size: 79 30 1640
Fragments arranged by size:
1640 79 30
BseYI C'CCAG_C
Cuts at: 0 1412 1749
Size: 1412 337
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 899 1118 1550 1749
Size: 899 219 432 199
Fragments arranged by size:
899 432 219 199
BsiHKAI G_wGCw'C
Cuts at: 0 1038 1749
Size: 1038 711
BsmI GAATG_Cn'
Cuts at: 0 812 1001 1749
Size: 812 189 748
Fragments arranged by size:
812 748 189
Bsp1286I G_dGCh'C
Cuts at: 0 172 202 1038 1749
Size: 172 30 836 711
Fragments arranged by size:
836 711 172 30
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1050 1522 1749
Size: 1050 472 227
Fragments arranged by size:
1050 472 227
BsrDI GCAATG_nn'
Cuts at: 0 552 792 840 1749
Size: 552 240 48 909
Fragments arranged by size:
909 552 240 48
BstAPI GCAn_nnn'nTGC
Cuts at: 0 223 886 1749
Size: 223 663 863
Fragments arranged by size:
863 663 223
BstBI TT'CG_AA
Cuts at: 0 1005 1749
Size: 1005 744
BstEII G'GTnAC_C
Cuts at: 0 613 1749
Size: 613 1136
BstYI r'GATC_y
Cuts at: 0 78 1749
Size: 78 1671
BtgI C'CryG_G
Cuts at: 0 148 1054 1749
Size: 148 906 695
Fragments arranged by size:
906 695 148
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1272 1749
Size: 1272 477
BtsI GCAGTG_nn'
Cuts at: 0 212 224 1749
Size: 212 12 1525
Fragments arranged by size:
1525 212 12
ClaI AT'CG_AT
Cuts at: 0 1553 1749
Size: 1553 196
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 720 755 1749
Size: 720 35 994
Fragments arranged by size:
994 720 35
DraI TTT'AAA
Cuts at: 0 302 1749
Size: 302 1447
EaeI y'GGCC_r
Cuts at: 0 1685 1749
Size: 1685 64
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 453 1435 1595 1749
Size: 453 982 160 154
Fragments arranged by size:
982 453 160 154
EcoO109I rG'GnC_Cy
Cuts at: 0 1579 1749
Size: 1579 170
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 406 1749
Size: 406 1343
HincII GTy'rAC
Cuts at: 0 259 1749
Size: 259 1490
HindIII A'AGCT_T
Cuts at: 0 4 240 1749
Size: 4 236 1509
Fragments arranged by size:
1509 236 4
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1099 1407 1749
Size: 1099 308 342
Fragments arranged by size:
1099 342 308
MscI TGG'CCA
Cuts at: 0 1687 1749
Size: 1687 62
MslI CAynn'nnrTG
Cuts at: 0 217 1452 1681 1749
Size: 217 1235 229 68
Fragments arranged by size:
1235 229 217 68
MspA1I CmG'CkG
Cuts at: 0 431 1749
Size: 431 1318
NcoI C'CATG_G
Cuts at: 0 148 1054 1749
Size: 148 906 695
Fragments arranged by size:
906 695 148
NdeI CA'TA_TG
Cuts at: 0 418 1539 1749
Size: 418 1121 210
Fragments arranged by size:
1121 418 210
NsiI A_TGCA'T
Cuts at: 0 163 1402 1749
Size: 163 1239 347
Fragments arranged by size:
1239 347 163
NspI r_CATG'y
Cuts at: 0 317 550 776 1400 1749
Size: 317 233 226 624 349
Fragments arranged by size:
624 349 317 233 226
PciI A'CATG_T
Cuts at: 0 546 1749
Size: 546 1203
PflMI CCAn_nnn'nTGG
Cuts at: 0 208 1749
Size: 208 1541
PshAI GACnn'nnGTC
Cuts at: 0 1615 1749
Size: 1615 134
PstI C_TGCA'G
Cuts at: 0 219 1749
Size: 219 1530
PvuII CAG'CTG
Cuts at: 0 431 1749
Size: 431 1318
SfcI C'TryA_G
Cuts at: 0 215 1749
Size: 215 1534
SmlI C'TyrA_G
Cuts at: 0 42 71 899 1749
Size: 42 29 828 850
Fragments arranged by size:
850 828 42 29
SphI G_CATG'C
Cuts at: 0 776 1749
Size: 776 973
StuI AGG'CCT
Cuts at: 0 1725 1749
Size: 1725 24
StyI C'CwwG_G
Cuts at: 0 148 468 777 1054 1749
Size: 148 320 309 277 695
Fragments arranged by size:
695 320 309 277 148
TatI w'GTAC_w
Cuts at: 0 586 1739 1749
Size: 586 1153 10
Fragments arranged by size:
1153 586 10
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 79 111 1749
Size: 79 32 1638
Fragments arranged by size:
1638 79 32
XmnI GAAnn'nnTTC
Cuts at: 0 278 1749
Size: 278 1471
Enzymes that do cut:
AarI AccI AcuI AflII AflIII AleI ApoI BaeI
BanI BbsI BciVI BfrBI BglI BlpI Bme1580I BmrI
BpmI BpuEI BsaI BsaAI BsaXI BseYI BsgI BsiHKAI
BsmI Bsp1286I BspMI BsrDI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI EaeI Eco57MI
EcoO109I FalI HincII HindIII MmeI MscI MslI MspA1I
NcoI NdeI NsiI NspI PciI PflMI PshAI PstI
PvuII SfcI SmlI SphI StuI StyI TatI TstI
XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AgeI AhdI AlfI AloI
AlwNI ApaI ApaLI AscI AseI AsiSI AvaI AvrII
BamHI BanII BbeI BbvCI BcgI BclI BglII BmgBI
BmtI BplI Bpu10I BsaBI BsaHI BsaWI BseRI BsiEI
BsiWI BsmBI BspEI BspHI BsrBI BsrFI BsrGI BssHII
BssSI BstXI BstZ17I Bsu36I DraIII DrdI EagI EarI
EciI EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI
HaeII Hin4I HpaI KasI KpnI MfeI MluI NaeI
NarI NgoMIV NheI NotI NruI PacI PasI PfoI
PmeI PmlI PpiI PpuMI PsiI PspOMI PspXI PsrI
PvuI RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SrfI SspI SwaI TaqII Tth111I XbaI XcmI
XhoI XmaI ZraI