Restriction map for
pFN21AE2005
(Linear) (Six-base) MAPSORT of: KIEE2005.seq Check: 9270 from: 1 to: 1743
KIEE2005.orf
With 182 enzymes: SgfI *
March 22, 2012 15:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 27 1587 1743
Size: 27 1560 156
Fragments arranged by size:
1560 156 27
AatII G_ACGT'C
Cuts at: 0 1172 1743
Size: 1172 571
AccI GT'mk_AC
Cuts at: 0 815 1743
Size: 815 928
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 565 1743
Size: 565 1178
AfeI AGC'GCT
Cuts at: 0 673 1743
Size: 673 1070
AflIII A'CryG_T
Cuts at: 0 647 1743
Size: 647 1096
AleI CACnn'nnGTG
Cuts at: 0 1369 1738 1743
Size: 1369 369 5
Fragments arranged by size:
1369 369 5
ApaI G_GGCC'C
Cuts at: 0 16 559 1743
Size: 16 543 1184
Fragments arranged by size:
1184 543 16
ApaLI G'TGCA_C
Cuts at: 0 282 1743
Size: 282 1461
AvaI C'yCGr_G
Cuts at: 0 9 84 476 483 559 582 775 1242
Size: 9 75 392 7 76 23 193 467
Cuts at: 1242 1743
Size: 501
Fragments arranged by size:
501 467 392 193 76 75 23 9
7
BanI G'GyrC_C
Cuts at: 0 447 850 1743
Size: 447 403 893
Fragments arranged by size:
893 447 403
BanII G_rGCy'C
Cuts at: 0 16 49 473 484 559 1139 1337 1743
Size: 16 33 424 11 75 580 198 406
Fragments arranged by size:
580 424 406 198 75 33 16 11
BbeI G_GCGC'C
Cuts at: 0 451 854 1743
Size: 451 403 889
Fragments arranged by size:
889 451 403
BbsI GAAGACnn'nnnn_
Cuts at: 0 927 1099 1288 1743
Size: 927 172 189 455
Fragments arranged by size:
927 455 189 172
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 114 148 1743
Size: 114 34 1595
Fragments arranged by size:
1595 114 34
BclI T'GATC_A
Cuts at: 0 1649 1743
Size: 1649 94
BglI GCCn_nnn'nGGC
Cuts at: 0 516 700 1084 1743
Size: 516 184 384 659
Fragments arranged by size:
659 516 384 184
BglII A'GATC_T
Cuts at: 0 1505 1520 1743
Size: 1505 15 223
Fragments arranged by size:
1505 223 15
Bme1580I G_kGCm'C
Cuts at: 0 16 286 559 1743
Size: 16 270 273 1184
Fragments arranged by size:
1184 273 270 16
BmgBI CAC'GTC
Cuts at: 0 648 1229 1743
Size: 648 581 514
Fragments arranged by size:
648 581 514
BmrI ACTGGGnnnn_n'
Cuts at: 0 1719 1743
Size: 1719 24
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 312 1743
Size: 312 1431
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 675 705 931 1330 1743
Size: 675 30 226 399 413
Fragments arranged by size:
675 413 399 226 30
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 328 1743
Size: 328 1415
BsaI GGTCTCn'nnnn_
Cuts at: 0 300 1743
Size: 300 1443
BsaAI yAC'GTr
Cuts at: 0 600 1155 1743
Size: 600 555 588
Fragments arranged by size:
600 588 555
BsaHI Gr'CG_yC
Cuts at: 0 448 851 1169 1743
Size: 448 403 318 574
Fragments arranged by size:
574 448 403 318
BsaWI w'CCGG_w
Cuts at: 0 1268 1743
Size: 1268 475
BseYI C'CCAG_C
Cuts at: 0 1679 1743
Size: 1679 64
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 615 909 1623 1743
Size: 615 294 714 120
Fragments arranged by size:
714 615 294 120
BsiEI CG_ry'CG
Cuts at: 0 511 873 1743
Size: 511 362 870
Fragments arranged by size:
870 511 362
BsiHKAI G_wGCw'C
Cuts at: 0 265 286 352 539 740 919 1139 1196
Size: 265 21 66 187 201 179 220 57
Cuts at: 1196 1743
Size: 547
Fragments arranged by size:
547 265 220 201 187 179 66 57
21
Bsp1286I G_dGCh'C
Cuts at: 0 16 49 265 286 352 473 484 539
Size: 16 33 216 21 66 121 11 55
Cuts at: 539 559 740 919 1139 1196 1337 1743
Size: 20 181 179 220 57 141 406
Fragments arranged by size:
406 220 216 181 179 141 121 66
57 55 33 21 20 16 11
BspEI T'CCGG_A
Cuts at: 0 1268 1743
Size: 1268 475
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 27 282 1587 1743
Size: 27 255 1305 156
Fragments arranged by size:
1305 255 156 27
BsrBI CCG'CTC
Cuts at: 0 627 1743
Size: 627 1116
BsrFI r'CCGG_y
Cuts at: 0 847 919 1012 1183 1359 1638 1743
Size: 847 72 93 171 176 279 105
Fragments arranged by size:
847 279 176 171 105 93 72
BsrGI T'GTAC_A
Cuts at: 0 1349 1743
Size: 1349 394
BssHII G'CGCG_C
Cuts at: 0 527 620 1743
Size: 527 93 1123
Fragments arranged by size:
1123 527 93
BssSI C'ACGA_G
Cuts at: 0 48 172 205 1030 1743
Size: 48 124 33 825 713
Fragments arranged by size:
825 713 124 48 33
BstAPI GCAn_nnn'nTGC
Cuts at: 0 866 923 1743
Size: 866 57 820
Fragments arranged by size:
866 820 57
BstXI CCAn_nnnn'nTGG
Cuts at: 0 75 165 1743
Size: 75 90 1578
Fragments arranged by size:
1578 90 75
BstYI r'GATC_y
Cuts at: 0 113 1418 1505 1520 1715 1743
Size: 113 1305 87 15 195 28
Fragments arranged by size:
1305 195 113 87 28 15
BtgI C'CryG_G
Cuts at: 0 68 237 821 1499 1701 1743
Size: 68 169 584 678 202 42
Fragments arranged by size:
678 584 202 169 68 42
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 846 1743
Size: 846 897
BtsI GCAGTG_nn'
Cuts at: 0 686 1743
Size: 686 1057
DraIII CAC_nnn'GTG
Cuts at: 0 600 1743
Size: 600 1143
DrdI GACnn_nn'nnGTC
Cuts at: 0 1584 1743
Size: 1584 159
EaeI y'GGCC_r
Cuts at: 0 240 508 692 882 962 1007 1361 1481
Size: 240 268 184 190 80 45 354 120
Cuts at: 1481 1640 1743
Size: 159 103
Fragments arranged by size:
354 268 240 190 184 159 120 103
80 45
EagI C'GGCC_G
Cuts at: 0 508 1743
Size: 508 1235
EarI CTCTTCn'nnn_
Cuts at: 0 601 847 1342 1618 1743
Size: 601 246 495 276 125
Fragments arranged by size:
601 495 276 246 125
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 826 1743
Size: 826 917
EcoICRI GAG'CTC
Cuts at: 0 1137 1743
Size: 1137 606
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 565 675 705 931 1330 1743
Size: 565 110 30 226 399 413
Fragments arranged by size:
565 413 399 226 110 30
EcoO109I rG'GnC_Cy
Cuts at: 0 13 555 556 1743
Size: 13 542 1 1187
Fragments arranged by size:
1187 542 13 1
FspI TGC'GCA
Cuts at: 0 1117 1743
Size: 1117 626
HaeII r_GCGC'y
Cuts at: 0 9 84 451 675 728 783 854 1743
Size: 9 75 367 224 53 55 71 889
Fragments arranged by size:
889 367 224 75 71 55 53 9
HincII GTy'rAC
Cuts at: 0 1173 1743
Size: 1173 570
KasI G'GCGC_C
Cuts at: 0 447 850 1743
Size: 447 403 893
Fragments arranged by size:
893 447 403
MscI TGG'CCA
Cuts at: 0 694 964 1743
Size: 694 270 779
Fragments arranged by size:
779 694 270
MslI CAynn'nnrTG
Cuts at: 0 73 1369 1738 1743
Size: 73 1296 369 5
Fragments arranged by size:
1296 369 73 5
MspA1I CmG'CkG
Cuts at: 0 219 501 516 543 881 1553 1658 1743
Size: 219 282 15 27 338 672 105 85
Fragments arranged by size:
672 338 282 219 105 85 27 15
NaeI GCC'GGC
Cuts at: 0 849 1014 1361 1743
Size: 849 165 347 382
Fragments arranged by size:
849 382 347 165
NarI GG'CG_CC
Cuts at: 0 448 851 1743
Size: 448 403 892
Fragments arranged by size:
892 448 403
NcoI C'CATG_G
Cuts at: 0 68 1743
Size: 68 1675
NgoMIV G'CCGG_C
Cuts at: 0 847 1012 1359 1743
Size: 847 165 347 384
Fragments arranged by size:
847 384 347 165
PflMI CCAn_nnn'nTGG
Cuts at: 0 600 1743
Size: 600 1143
PfoI T'CCnGG_A
Cuts at: 0 899 1743
Size: 899 844
PmlI CAC'GTG
Cuts at: 0 600 1743
Size: 600 1143
PspOMI G'GGCC_C
Cuts at: 0 12 555 1743
Size: 12 543 1188
Fragments arranged by size:
1188 543 12
PstI C_TGCA'G
Cuts at: 0 38 1586 1743
Size: 38 1548 157
Fragments arranged by size:
1548 157 38
SacI G_AGCT'C
Cuts at: 0 1139 1743
Size: 1139 604
SapI GCTCTTCn'nnn_
Cuts at: 0 601 1342 1618 1743
Size: 601 741 276 125
Fragments arranged by size:
741 601 276 125
SbfI CC_TGCA'GG
Cuts at: 0 38 1743
Size: 38 1705
SexAI A'CCwGG_T
Cuts at: 0 997 1214 1743
Size: 997 217 529
Fragments arranged by size:
997 529 217
SfcI C'TryA_G
Cuts at: 0 34 1582 1743
Size: 34 1548 161
Fragments arranged by size:
1548 161 34
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 516 1743
Size: 516 1227
SfoI GGC'GCC
Cuts at: 0 449 852 1743
Size: 449 403 891
Fragments arranged by size:
891 449 403
SgrAI Cr'CCGG_yG
Cuts at: 0 847 1743
Size: 847 896
SmaI CCC'GGG
Cuts at: 0 11 86 478 561 584 1743
Size: 11 75 392 83 23 1159
Fragments arranged by size:
1159 392 83 75 23 11
SmlI C'TyrA_G
Cuts at: 0 343 1743
Size: 343 1400
SrfI GCCC'GGGC
Cuts at: 0 11 86 1743
Size: 11 75 1657
Fragments arranged by size:
1657 75 11
StyI C'CwwG_G
Cuts at: 0 68 1743
Size: 68 1675
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 969 1743
Size: 969 774
TatI w'GTAC_w
Cuts at: 0 650 1349 1743
Size: 650 699 394
Fragments arranged by size:
699 650 394
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 66 1104 1497 1743
Size: 66 1038 393 246
Fragments arranged by size:
1038 393 246 66
XmaI C'CCGG_G
Cuts at: 0 9 84 476 559 582 1743
Size: 9 75 392 83 23 1161
Fragments arranged by size:
1161 392 83 75 23 9
ZraI GAC'GTC
Cuts at: 0 1170 1743
Size: 1170 573
Enzymes that do cut:
AarI AatII AccI AcuI AfeI AflIII AleI ApaI
ApaLI AvaI BanI BanII BbeI BbsI BcgI BclI
BglI BglII Bme1580I BmgBI BmrI BplI BpmI BpuEI
BsaI BsaAI BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspMI BsrBI BsrFI BsrGI BssHII BssSI
BstAPI BstXI BstYI BtgI BtgZI BtsI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I FspI
HaeII HincII KasI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV PflMI PfoI PmlI PspOMI PstI SacI
SapI SbfI SexAI SfcI SfiI SfoI SgrAI SmaI
SmlI SrfI StyI TaqII TatI XcmI XmaI ZraI
Enzymes that do not cut:
Acc65I AclI AflII AgeI AhdI AlfI AloI AlwNI
ApoI AscI AseI AsiSI AvrII BaeI BamHI BbvCI
BciVI BfrBI BlpI BmtI Bpu10I BsaBI BsaXI BseRI
BsiWI BsmI BsmBI BspHI BsrDI BstBI BstEII BstZ17I
Bsu36I ClaI CspCI DraI EcoNI EcoRI EcoRV FalI
FseI FspAI Hin4I HindIII HpaI KpnI MfeI MluI
MmeI NdeI NheI NotI NruI NsiI NspI PacI
PasI PciI PmeI PpiI PpuMI PshAI PsiI PspXI
PsrI PvuI PvuII RsrII SacII SalI SanDI ScaI
SgfI SnaBI SpeI SphI SspI StuI SwaI TstI
Tth111I XbaI XhoI XmnI