Restriction map for
pFN21AE2004
(Linear) (Six-base) MAPSORT of: KIEE2004.seq Check: 8424 from: 1 to: 1737
KIEE2004.orf
With 182 enzymes: SgfI *
July 15, 2010 18:52 ..
AccI GT'mk_AC
Cuts at: 0 1578 1737
Size: 1578 159
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 336 805 932 1669 1737
Size: 336 469 127 737 68
Fragments arranged by size:
737 469 336 127 68
AfeI AGC'GCT
Cuts at: 0 502 1737
Size: 502 1235
AflIII A'CryG_T
Cuts at: 0 239 1737
Size: 239 1498
AleI CACnn'nnGTG
Cuts at: 0 67 1567 1737
Size: 67 1500 170
Fragments arranged by size:
1500 170 67
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 85 1737
Size: 85 1652
AlwNI CAG_nnn'CTG
Cuts at: 0 1065 1482 1602 1737
Size: 1065 417 120 135
Fragments arranged by size:
1065 417 135 120
ApaI G_GGCC'C
Cuts at: 0 227 976 1438 1737
Size: 227 749 462 299
Fragments arranged by size:
749 462 299 227
AvaI C'yCGr_G
Cuts at: 0 219 276 1737
Size: 219 57 1461
Fragments arranged by size:
1461 219 57
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 833 866 1737
Size: 833 33 871
Fragments arranged by size:
871 833 33
BanI G'GyrC_C
Cuts at: 0 272 1069 1737
Size: 272 797 668
Fragments arranged by size:
797 668 272
BanII G_rGCy'C
Cuts at: 0 38 227 599 976 1259 1414 1438 1737
Size: 38 189 372 377 283 155 24 299
Fragments arranged by size:
377 372 299 283 189 155 38 24
BbsI GAAGACnn'nnnn_
Cuts at: 0 1026 1737
Size: 1026 711
BciVI GTATCCnnnnn_n'
Cuts at: 0 834 1737
Size: 834 903
BglI GCCn_nnn'nGGC
Cuts at: 0 13 413 1737
Size: 13 400 1324
Fragments arranged by size:
1324 400 13
BlpI GC'TnA_GC
Cuts at: 0 1258 1606 1737
Size: 1258 348 131
Fragments arranged by size:
1258 348 131
Bme1580I G_kGCm'C
Cuts at: 0 227 275 976 1074 1438 1737
Size: 227 48 701 98 364 299
Fragments arranged by size:
701 364 299 227 98 48
BmgBI CAC'GTC
Cuts at: 0 1140 1737
Size: 1140 597
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1005 1737
Size: 1005 732
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 583 1737
Size: 583 1154
Bpu10I CC'TnA_GC
Cuts at: 0 385 1669 1737
Size: 385 1284 68
Fragments arranged by size:
1284 385 68
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 74 598 1504 1737
Size: 74 524 906 233
Fragments arranged by size:
906 524 233 74
BsaI GGTCTCn'nnnn_
Cuts at: 0 295 1737
Size: 295 1442
BsaAI yAC'GTr
Cuts at: 0 1269 1287 1737
Size: 1269 18 450
Fragments arranged by size:
1269 450 18
BsaWI w'CCGG_w
Cuts at: 0 377 533 1737
Size: 377 156 1204
Fragments arranged by size:
1204 377 156
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1276 1306 1737
Size: 1276 30 431
Fragments arranged by size:
1276 431 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 931 934 978 1243 1284 1540 1642 1737
Size: 931 3 44 265 41 256 102 95
Fragments arranged by size:
931 265 256 102 95 44 41 3
BseYI C'CCAG_C
Cuts at: 0 334 344 567 893 1737
Size: 334 10 223 326 844
Fragments arranged by size:
844 334 326 223 10
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 45 58 745 1291 1737
Size: 45 13 687 546 446
Fragments arranged by size:
687 546 446 45 13
BsiEI CG_ry'CG
Cuts at: 0 836 1737
Size: 836 901
BsiHKAI G_wGCw'C
Cuts at: 0 38 599 657 831 1292 1737
Size: 38 561 58 174 461 445
Fragments arranged by size:
561 461 445 174 58 38
BsmI GAATG_Cn'
Cuts at: 0 9 152 1737
Size: 9 143 1585
Fragments arranged by size:
1585 143 9
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1130 1737
Size: 1130 607
Bsp1286I G_dGCh'C
Cuts at: 0 38 227 275 599 657 831 976 1074
Size: 38 189 48 324 58 174 145 98
Cuts at: 1074 1259 1292 1414 1438 1737
Size: 185 33 122 24 299
Fragments arranged by size:
324 299 189 185 174 145 122 98
58 48 38 33 24
BspEI T'CCGG_A
Cuts at: 0 377 1737
Size: 377 1360
BspHI T'CATG_A
Cuts at: 0 524 1737
Size: 524 1213
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1050 1737
Size: 1050 687
BsrBI CCG'CTC
Cuts at: 0 716 1737
Size: 716 1021
BsrFI r'CCGG_y
Cuts at: 0 254 800 1737
Size: 254 546 937
Fragments arranged by size:
937 546 254
BstEII G'GTnAC_C
Cuts at: 0 1398 1737
Size: 1398 339
BstXI CCAn_nnnn'nTGG
Cuts at: 0 528 1737
Size: 528 1209
BstYI r'GATC_y
Cuts at: 0 536 1274 1737
Size: 536 738 463
Fragments arranged by size:
738 536 463
BtgI C'CryG_G
Cuts at: 0 39 1187 1700 1737
Size: 39 1148 513 37
Fragments arranged by size:
1148 513 39 37
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 710 1737
Size: 710 1027
ClaI AT'CG_AT
Cuts at: 0 590 1737
Size: 590 1147
DraIII CAC_nnn'GTG
Cuts at: 0 652 1353 1737
Size: 652 701 384
Fragments arranged by size:
701 652 384
DrdI GACnn_nn'nnGTC
Cuts at: 0 1683 1737
Size: 1683 54
EaeI y'GGCC_r
Cuts at: 0 117 405 485 1737
Size: 117 288 80 1252
Fragments arranged by size:
1252 288 117 80
EarI CTCTTCn'nnn_
Cuts at: 0 61 134 355 872 908 1737
Size: 61 73 221 517 36 829
Fragments arranged by size:
829 517 221 73 61 36
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 148 352 1737
Size: 148 204 1385
Fragments arranged by size:
1385 204 148
EcoICRI GAG'CTC
Cuts at: 0 36 597 1737
Size: 36 561 1140
Fragments arranged by size:
1140 561 36
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 336 583 805 932 1669 1737
Size: 336 247 222 127 737 68
Fragments arranged by size:
737 336 247 222 127 68
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1522 1737
Size: 1522 215
EcoO109I rG'GnC_Cy
Cuts at: 0 223 415 972 1062 1435 1737
Size: 223 192 557 90 373 302
Fragments arranged by size:
557 373 302 223 192 90
FspI TGC'GCA
Cuts at: 0 179 1737
Size: 179 1558
FspAI rTGC'GCAy
Cuts at: 0 179 1737
Size: 179 1558
HaeII r_GCGC'y
Cuts at: 0 504 613 1737
Size: 504 109 1124
Fragments arranged by size:
1124 504 109
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 150 182 1357 1389 1737
Size: 150 32 1175 32 348
Fragments arranged by size:
1175 348 150 32 32
HincII GTy'rAC
Cuts at: 0 1579 1737
Size: 1579 158
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 148 215 856 1737
Size: 148 67 641 881
Fragments arranged by size:
881 641 148 67
MscI TGG'CCA
Cuts at: 0 119 487 1737
Size: 119 368 1250
Fragments arranged by size:
1250 368 119
MslI CAynn'nnrTG
Cuts at: 0 67 157 523 526 1567 1737
Size: 67 90 366 3 1041 170
Fragments arranged by size:
1041 366 170 90 67 3
NaeI GCC'GGC
Cuts at: 0 802 1737
Size: 802 935
NgoMIV G'CCGG_C
Cuts at: 0 800 1737
Size: 800 937
NspI r_CATG'y
Cuts at: 0 243 1089 1737
Size: 243 846 648
Fragments arranged by size:
846 648 243
PasI CC'CwG_GG
Cuts at: 0 969 1737
Size: 969 768
PciI A'CATG_T
Cuts at: 0 239 1737
Size: 239 1498
PflMI CCAn_nnn'nTGG
Cuts at: 0 1353 1737
Size: 1353 384
PfoI T'CCnGG_A
Cuts at: 0 818 1737
Size: 818 919
PmlI CAC'GTG
Cuts at: 0 1269 1287 1737
Size: 1269 18 450
Fragments arranged by size:
1269 450 18
PpuMI rG'GwC_Cy
Cuts at: 0 1062 1737
Size: 1062 675
PspOMI G'GGCC_C
Cuts at: 0 223 972 1434 1737
Size: 223 749 462 303
Fragments arranged by size:
749 462 303 223
PstI C_TGCA'G
Cuts at: 0 270 1425 1737
Size: 270 1155 312
Fragments arranged by size:
1155 312 270
RsrII CG'GwC_CG
Cuts at: 0 1704 1737
Size: 1704 33
SacI G_AGCT'C
Cuts at: 0 38 599 1737
Size: 38 561 1138
Fragments arranged by size:
1138 561 38
SalI G'TCGA_C
Cuts at: 0 1577 1737
Size: 1577 160
SapI GCTCTTCn'nnn_
Cuts at: 0 355 1737
Size: 355 1382
ScaI AGT'ACT
Cuts at: 0 1169 1737
Size: 1169 568
SexAI A'CCwGG_T
Cuts at: 0 1115 1737
Size: 1115 622
SfcI C'TryA_G
Cuts at: 0 266 1415 1421 1737
Size: 266 1149 6 316
Fragments arranged by size:
1149 316 266 6
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 413 1737
Size: 413 1324
SmaI CCC'GGG
Cuts at: 0 221 278 1737
Size: 221 57 1459
Fragments arranged by size:
1459 221 57
SmlI C'TyrA_G
Cuts at: 0 89 613 1519 1737
Size: 89 524 906 218
Fragments arranged by size:
906 524 218 89
SphI G_CATG'C
Cuts at: 0 1089 1737
Size: 1089 648
StuI AGG'CCT
Cuts at: 0 370 1234 1737
Size: 370 864 503
Fragments arranged by size:
864 503 370
StyI C'CwwG_G
Cuts at: 0 845 1565 1737
Size: 845 720 172
Fragments arranged by size:
845 720 172
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 823 850 1057 1442 1606 1737
Size: 823 27 207 385 164 131
Fragments arranged by size:
823 385 207 164 131 27
TatI w'GTAC_w
Cuts at: 0 946 1167 1737
Size: 946 221 570
Fragments arranged by size:
946 570 221
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1276 1308 1681 1713 1737
Size: 1276 32 373 32 24
Fragments arranged by size:
1276 373 32 32 24
Tth111I GACn'n_nGTC
Cuts at: 0 1147 1636 1737
Size: 1147 489 101
Fragments arranged by size:
1147 489 101
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1185 1737
Size: 1185 552
XmaI C'CCGG_G
Cuts at: 0 219 276 1737
Size: 219 57 1461
Fragments arranged by size:
1461 219 57
XmnI GAAnn'nnTTC
Cuts at: 0 1169 1737
Size: 1169 568
Enzymes that do cut:
AccI AcuI AfeI AflIII AleI AlfI AlwNI ApaI
AvaI BaeI BanI BanII BbsI BciVI BglI BlpI
Bme1580I BmgBI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrFI BstEII
BstXI BstYI BtgI BtgZI ClaI DraIII DrdI EaeI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FspI FspAI
HaeII Hin4I HincII MmeI MscI MslI NaeI NgoMIV
NspI PasI PciI PflMI PfoI PmlI PpuMI PspOMI
PstI RsrII SacI SalI SapI ScaI SexAI SfcI
SfiI SmaI SmlI SphI StuI StyI TaqII TatI
TstI Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AgeI AhdI AloI
ApaLI ApoI AscI AseI AsiSI AvrII BamHI BbeI
BbvCI BcgI BclI BfrBI BglII BmrI BmtI BsaBI
BsaHI BsiWI BsrDI BsrGI BssHII BssSI BstAPI BstBI
BstZ17I Bsu36I BtsI CspCI DraI EagI EcoRI EcoRV
FalI FseI HindIII HpaI KasI KpnI MfeI MluI
MspA1I NarI NcoI NdeI NheI NotI NruI NsiI
PacI PmeI PpiI PshAI PsiI PspXI PsrI PvuI
PvuII SacII SanDI SbfI SfoI SgfI SgrAI SnaBI
SpeI SrfI SspI SwaI XbaI XhoI ZraI