Restriction map for
pFN21AE2003
(Linear) (Six-base) MAPSORT of: KIEE2003.seq Check: 8439 from: 1 to: 1734
KIEE2003.orf
With 182 enzymes: SgfI *
July 3, 2012 17:06 ..
AatII G_ACGT'C
Cuts at: 0 195 1433 1734
Size: 195 1238 301
Fragments arranged by size:
1238 301 195
AccI GT'mk_AC
Cuts at: 0 926 1087 1734
Size: 926 161 647
Fragments arranged by size:
926 647 161
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 183 418 1085 1344 1656 1734
Size: 183 235 667 259 312 78
Fragments arranged by size:
667 312 259 235 183 78
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1010 1042 1734
Size: 1010 32 692
Fragments arranged by size:
1010 692 32
ApoI r'AATT_y
Cuts at: 0 244 710 1734
Size: 244 466 1024
Fragments arranged by size:
1024 466 244
AvaI C'yCGr_G
Cuts at: 0 7 1734
Size: 7 1727
BanI G'GyrC_C
Cuts at: 0 1059 1734
Size: 1059 675
BanII G_rGCy'C
Cuts at: 0 8 1391 1734
Size: 8 1383 343
Fragments arranged by size:
1383 343 8
BbsI GAAGACnn'nnnn_
Cuts at: 0 195 1496 1734
Size: 195 1301 238
Fragments arranged by size:
1301 238 195
BglII A'GATC_T
Cuts at: 0 1608 1734
Size: 1608 126
BmrI ACTGGGnnnn_n'
Cuts at: 0 1697 1734
Size: 1697 37
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1467 1734
Size: 1467 267
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 350 736 1390 1405 1734
Size: 350 386 654 15 329
Fragments arranged by size:
654 386 350 329 15
BsaHI Gr'CG_yC
Cuts at: 0 192 1430 1734
Size: 192 1238 304
Fragments arranged by size:
1238 304 192
BsaWI w'CCGG_w
Cuts at: 0 977 1734
Size: 977 757
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 93 123 1734
Size: 93 30 1611
Fragments arranged by size:
1611 93 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 418 1201 1234 1734
Size: 418 783 33 500
Fragments arranged by size:
783 500 418 33
BseYI C'CCAG_C
Cuts at: 0 1296 1321 1734
Size: 1296 25 413
Fragments arranged by size:
1296 413 25
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 144 1734
Size: 144 1590
BsiHKAI G_wGCw'C
Cuts at: 0 8 791 1734
Size: 8 783 943
Fragments arranged by size:
943 783 8
BsmBI CGTCTCn'nnnn_
Cuts at: 0 199 1734
Size: 199 1535
Bsp1286I G_dGCh'C
Cuts at: 0 8 791 1391 1734
Size: 8 783 600 343
Fragments arranged by size:
783 600 343 8
BspEI T'CCGG_A
Cuts at: 0 977 1734
Size: 977 757
BsrBI CCG'CTC
Cuts at: 0 1318 1734
Size: 1318 416
BstYI r'GATC_y
Cuts at: 0 1007 1466 1608 1734
Size: 1007 459 142 126
Fragments arranged by size:
1007 459 142 126
BstZ17I GTA'TAC
Cuts at: 0 927 1088 1734
Size: 927 161 646
Fragments arranged by size:
927 646 161
BtgI C'CryG_G
Cuts at: 0 1694 1734
Size: 1694 40
BtsI GCAGTG_nn'
Cuts at: 0 103 132 263 1734
Size: 103 29 131 1471
Fragments arranged by size:
1471 131 103 29
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 51 86 1734
Size: 51 35 1648
Fragments arranged by size:
1648 51 35
DraIII CAC_nnn'GTG
Cuts at: 0 1341 1734
Size: 1341 393
DrdI GACnn_nn'nnGTC
Cuts at: 0 1118 1734
Size: 1118 616
EaeI y'GGCC_r
Cuts at: 0 20 998 1734
Size: 20 978 736
Fragments arranged by size:
978 736 20
EarI CTCTTCn'nnn_
Cuts at: 0 202 667 1029 1671 1675 1734
Size: 202 465 362 642 4 59
Fragments arranged by size:
642 465 362 202 59 4
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 602 1734
Size: 602 1132
EcoICRI GAG'CTC
Cuts at: 0 6 1734
Size: 6 1728
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 183 350 418 736 1085 1344 1390 1405
Size: 183 167 68 318 349 259 46 15
Cuts at: 1405 1656 1734
Size: 251 78
Fragments arranged by size:
349 318 259 251 183 167 78 68
46 15
EcoRI G'AATT_C
Cuts at: 0 244 710 1734
Size: 244 466 1024
Fragments arranged by size:
1024 466 244
EcoRV GAT'ATC
Cuts at: 0 1440 1734
Size: 1440 294
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 93 125 296 328 1237 1269 1734
Size: 93 32 171 32 909 32 465
Fragments arranged by size:
909 465 171 93 32 32 32
HindIII A'AGCT_T
Cuts at: 0 1595 1734
Size: 1595 139
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1020 1734
Size: 1020 714
MscI TGG'CCA
Cuts at: 0 22 1000 1734
Size: 22 978 734
Fragments arranged by size:
978 734 22
MslI CAynn'nnrTG
Cuts at: 0 796 1734
Size: 796 938
NcoI C'CATG_G
Cuts at: 0 1694 1734
Size: 1694 40
NspI r_CATG'y
Cuts at: 0 1383 1734
Size: 1383 351
PflMI CCAn_nnn'nTGG
Cuts at: 0 1532 1734
Size: 1532 202
PfoI T'CCnGG_A
Cuts at: 0 1433 1734
Size: 1433 301
PshAI GACnn'nnGTC
Cuts at: 0 1696 1734
Size: 1696 38
PstI C_TGCA'G
Cuts at: 0 882 1264 1306 1734
Size: 882 382 42 428
Fragments arranged by size:
882 428 382 42
SacI G_AGCT'C
Cuts at: 0 8 1734
Size: 8 1726
SfcI C'TryA_G
Cuts at: 0 878 1260 1302 1662 1734
Size: 878 382 42 360 72
Fragments arranged by size:
878 382 360 72 42
SmlI C'TyrA_G
Cuts at: 0 7 1734
Size: 7 1727
SspI AAT'ATT
Cuts at: 0 951 1734
Size: 951 783
StyI C'CwwG_G
Cuts at: 0 1694 1734
Size: 1694 40
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 305 1547 1734
Size: 305 1242 187
Fragments arranged by size:
1242 305 187
TatI w'GTAC_w
Cuts at: 0 436 1734
Size: 436 1298
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1266 1298 1734
Size: 1266 32 436
Fragments arranged by size:
1266 436 32
XbaI T'CTAG_A
Cuts at: 0 1611 1734
Size: 1611 123
XhoI C'TCGA_G
Cuts at: 0 7 1734
Size: 7 1727
XmnI GAAnn'nnTTC
Cuts at: 0 1136 1734
Size: 1136 598
ZraI GAC'GTC
Cuts at: 0 193 1431 1734
Size: 193 1238 303
Fragments arranged by size:
1238 303 193
Enzymes that do cut:
AatII AccI AcuI AloI ApoI AvaI BanI BanII
BbsI BglII BmrI BplI BpmI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspEI BsrBI
BstYI BstZ17I BtgI BtsI CspCI DraIII DrdI EaeI
EarI EciI EcoICRI Eco57MI EcoRI EcoRV Hin4I HindIII
MmeI MscI MslI NcoI NspI PflMI PfoI PshAI
PstI SacI SfcI SmlI SspI StyI TaqII TatI
TstI XbaI XhoI XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AlwNI ApaI ApaLI AscI AseI AsiSI
AvrII BaeI BamHI BbeI BbvCI BcgI BciVI BclI
BfrBI BglI BlpI Bme1580I BmgBI BmtI Bpu10I BpuEI
BsaI BsaAI BsaBI BsiEI BsiWI BsmI BspHI BspMI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI Bsu36I BtgZI ClaI DraI EagI EcoNI EcoO109I
FalI FseI FspI FspAI HaeII HincII HpaI KasI
KpnI MfeI MluI MspA1I NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PciI PmeI
PmlI PpiI PpuMI PsiI PspOMI PspXI PsrI PvuI
PvuII RsrII SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI StuI SwaI Tth111I XcmI XmaI