Restriction map for
pFN21AE1996
(Linear) (Six-base) MAPSORT of: KIEE1996.seq Check: 8070 from: 1 to: 1725
KIEE1996.orf
With 182 enzymes: SgfI *
July 15, 2010 18:52 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 853 1725
Size: 853 872
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 52 772 1367 1393 1725
Size: 52 720 595 26 332
Fragments arranged by size:
720 595 332 52 26
AfeI AGC'GCT
Cuts at: 0 1378 1725
Size: 1378 347
AflIII A'CryG_T
Cuts at: 0 1694 1725
Size: 1694 31
AgeI A'CCGG_T
Cuts at: 0 821 1725
Size: 821 904
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 34 1111 1725
Size: 34 1077 614
Fragments arranged by size:
1077 614 34
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 37 69 1561 1593 1725
Size: 37 32 1492 32 132
Fragments arranged by size:
1492 132 37 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1323 1371 1611 1725
Size: 1323 48 240 114
Fragments arranged by size:
1323 240 114 48
ApaI G_GGCC'C
Cuts at: 0 241 1725
Size: 241 1484
ApoI r'AATT_y
Cuts at: 0 925 1725
Size: 925 800
AvaI C'yCGr_G
Cuts at: 0 124 144 163 219 229 735 1237 1489
Size: 124 20 19 56 10 506 502 252
Cuts at: 1489 1725
Size: 236
Fragments arranged by size:
506 502 252 236 124 56 20 19
10
BanI G'GyrC_C
Cuts at: 0 120 256 295 553 1216 1656 1672 1725
Size: 120 136 39 258 663 440 16 53
Fragments arranged by size:
663 440 258 136 120 53 39 16
BanII G_rGCy'C
Cuts at: 0 212 241 577 670 868 1238 1496 1565
Size: 212 29 336 93 198 370 258 69
Cuts at: 1565 1592 1725
Size: 27 133
Fragments arranged by size:
370 336 258 212 198 133 93 69
29 27
BbeI G_GCGC'C
Cuts at: 0 124 260 557 1220 1660 1676 1725
Size: 124 136 297 663 440 16 49
Fragments arranged by size:
663 440 297 136 124 49 16
BbsI GAAGACnn'nnnn_
Cuts at: 0 1160 1389 1725
Size: 1160 229 336
Fragments arranged by size:
1160 336 229
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1266 1300 1725
Size: 1266 34 425
Fragments arranged by size:
1266 425 34
BglI GCCn_nnn'nGGC
Cuts at: 0 207 1288 1725
Size: 207 1081 437
Fragments arranged by size:
1081 437 207
BglII A'GATC_T
Cuts at: 0 1454 1725
Size: 1454 271
BlpI GC'TnA_GC
Cuts at: 0 67 1591 1725
Size: 67 1524 134
Fragments arranged by size:
1524 134 67
Bme1580I G_kGCm'C
Cuts at: 0 241 1725
Size: 241 1484
BmgBI CAC'GTC
Cuts at: 0 720 1725
Size: 720 1005
BmrI ACTGGGnnnn_n'
Cuts at: 0 1512 1725
Size: 1512 213
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 789 1473 1677 1725
Size: 789 684 204 48
Fragments arranged by size:
789 684 204 48
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 249 604 913 1725
Size: 249 355 309 812
Fragments arranged by size:
812 355 309 249
Bpu10I CC'TnA_GC
Cuts at: 0 1465 1725
Size: 1465 260
BsaI GGTCTCn'nnnn_
Cuts at: 0 1543 1725
Size: 1543 182
BsaHI Gr'CG_yC
Cuts at: 0 121 257 399 554 760 1217 1657 1673
Size: 121 136 142 155 206 457 440 16
Cuts at: 1673 1725
Size: 52
Fragments arranged by size:
457 440 206 155 142 136 121 52
16
BsaWI w'CCGG_w
Cuts at: 0 821 1725
Size: 821 904
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1561 1591 1725
Size: 1561 30 134
Fragments arranged by size:
1561 134 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 876 910 913 1267 1537 1561 1725
Size: 876 34 3 354 270 24 164
Fragments arranged by size:
876 354 270 164 34 24 3
BseYI C'CCAG_C
Cuts at: 0 202 1668 1725
Size: 202 1466 57
Fragments arranged by size:
1466 202 57
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 709 1117 1725
Size: 709 408 608
Fragments arranged by size:
709 608 408
BsiEI CG_ry'CG
Cuts at: 0 1520 1725
Size: 1520 205
BsiHKAI G_wGCw'C
Cuts at: 0 451 868 1238 1496 1592 1725
Size: 451 417 370 258 96 133
Fragments arranged by size:
451 417 370 258 133 96
BsiWI C'GTAC_G
Cuts at: 0 935 1725
Size: 935 790
BsmI GAATG_Cn'
Cuts at: 0 371 1725
Size: 371 1354
Bsp1286I G_dGCh'C
Cuts at: 0 212 241 451 577 670 868 1238 1496
Size: 212 29 210 126 93 198 370 258
Cuts at: 1496 1565 1592 1725
Size: 69 27 133
Fragments arranged by size:
370 258 212 210 198 133 126 93
69 29 27
BspHI T'CATG_A
Cuts at: 0 662 1725
Size: 662 1063
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 853 1001 1725
Size: 853 148 724
Fragments arranged by size:
853 724 148
BsrBI CCG'CTC
Cuts at: 0 186 543 1725
Size: 186 357 1182
Fragments arranged by size:
1182 357 186
BsrFI r'CCGG_y
Cuts at: 0 24 556 821 1725
Size: 24 532 265 904
Fragments arranged by size:
904 532 265 24
BssSI C'ACGA_G
Cuts at: 0 360 469 1082 1438 1725
Size: 360 109 613 356 287
Fragments arranged by size:
613 360 356 287 109
BstAPI GCAn_nnn'nTGC
Cuts at: 0 90 1725
Size: 90 1635
BstYI r'GATC_y
Cuts at: 0 304 803 1061 1454 1725
Size: 304 499 258 393 271
Fragments arranged by size:
499 393 304 271 258
BtgI C'CryG_G
Cuts at: 0 482 788 1553 1598 1676 1725
Size: 482 306 765 45 78 49
Fragments arranged by size:
765 482 306 78 49 45
BtsI GCAGTG_nn'
Cuts at: 0 1264 1725
Size: 1264 461
EaeI y'GGCC_r
Cuts at: 0 356 503 641 1280 1388 1517 1541 1725
Size: 356 147 138 639 108 129 24 184
Fragments arranged by size:
639 356 184 147 138 129 108 24
EagI C'GGCC_G
Cuts at: 0 1517 1725
Size: 1517 208
EarI CTCTTCn'nnn_
Cuts at: 0 335 550 1725
Size: 335 215 1175
Fragments arranged by size:
1175 335 215
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 919 1725
Size: 919 806
EcoICRI GAG'CTC
Cuts at: 0 866 1236 1494 1590 1725
Size: 866 370 258 96 135
Fragments arranged by size:
866 370 258 135 96
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 52 249 604 772 913 1367 1393 1725
Size: 52 197 355 168 141 454 26 332
Fragments arranged by size:
454 355 332 197 168 141 52 26
EcoNI CCTnn'n_nnAGG
Cuts at: 0 688 754 1725
Size: 688 66 971
Fragments arranged by size:
971 688 66
EcoO109I rG'GnC_Cy
Cuts at: 0 167 238 1290 1368 1643 1710 1725
Size: 167 71 1052 78 275 67 15
Fragments arranged by size:
1052 275 167 78 71 67 15
EcoRI G'AATT_C
Cuts at: 0 925 1725
Size: 925 800
HaeII r_GCGC'y
Cuts at: 0 124 260 557 889 1220 1380 1660 1676
Size: 124 136 297 332 331 160 280 16
Cuts at: 1676 1725
Size: 49
Fragments arranged by size:
332 331 297 280 160 136 124 49
16
HincII GTy'rAC
Cuts at: 0 417 1725
Size: 417 1308
KasI G'GCGC_C
Cuts at: 0 120 256 553 1216 1656 1672 1725
Size: 120 136 297 663 440 16 53
Fragments arranged by size:
663 440 297 136 120 53 16
MluI A'CGCG_T
Cuts at: 0 1694 1725
Size: 1694 31
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 400 1725
Size: 400 1325
MscI TGG'CCA
Cuts at: 0 505 1282 1390 1725
Size: 505 777 108 335
Fragments arranged by size:
777 505 335 108
MslI CAynn'nnrTG
Cuts at: 0 1537 1725
Size: 1537 188
MspA1I CmG'CkG
Cuts at: 0 87 206 801 918 1193 1481 1632 1668
Size: 87 119 595 117 275 288 151 36
Cuts at: 1668 1722 1725
Size: 54 3
Fragments arranged by size:
595 288 275 151 119 117 87 54
36 3
NaeI GCC'GGC
Cuts at: 0 558 1725
Size: 558 1167
NarI GG'CG_CC
Cuts at: 0 121 257 554 1217 1657 1673 1725
Size: 121 136 297 663 440 16 52
Fragments arranged by size:
663 440 297 136 121 52 16
NcoI C'CATG_G
Cuts at: 0 482 1725
Size: 482 1243
NgoMIV G'CCGG_C
Cuts at: 0 556 1725
Size: 556 1169
PasI CC'CwG_GG
Cuts at: 0 252 1565 1725
Size: 252 1313 160
Fragments arranged by size:
1313 252 160
PflMI CCAn_nnn'nTGG
Cuts at: 0 1323 1725
Size: 1323 402
PfoI T'CCnGG_A
Cuts at: 0 1301 1725
Size: 1301 424
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1171 1203 1561 1593 1725
Size: 1171 32 358 32 132
Fragments arranged by size:
1171 358 132 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 1368 1710 1725
Size: 1368 342 15
Fragments arranged by size:
1368 342 15
PspOMI G'GGCC_C
Cuts at: 0 237 1725
Size: 237 1488
PspXI vC'TCGA_Gb
Cuts at: 0 1237 1725
Size: 1237 488
PstI C_TGCA'G
Cuts at: 0 23 98 878 1367 1725
Size: 23 75 780 489 358
Fragments arranged by size:
780 489 358 75 23
PvuII CAG'CTG
Cuts at: 0 918 1725
Size: 918 807
SacI G_AGCT'C
Cuts at: 0 868 1238 1496 1592 1725
Size: 868 370 258 96 133
Fragments arranged by size:
868 370 258 133 96
SapI GCTCTTCn'nnn_
Cuts at: 0 335 550 1725
Size: 335 215 1175
Fragments arranged by size:
1175 335 215
ScaI AGT'ACT
Cuts at: 0 1183 1243 1725
Size: 1183 60 482
Fragments arranged by size:
1183 482 60
SexAI A'CCwGG_T
Cuts at: 0 683 1328 1725
Size: 683 645 397
Fragments arranged by size:
683 645 397
SfcI C'TryA_G
Cuts at: 0 19 94 874 1363 1725
Size: 19 75 780 489 362
Fragments arranged by size:
780 489 362 75 19
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1288 1725
Size: 1288 437
SfoI GGC'GCC
Cuts at: 0 122 258 555 1218 1658 1674 1725
Size: 122 136 297 663 440 16 51
Fragments arranged by size:
663 440 297 136 122 51 16
SgrAI Cr'CCGG_yG
Cuts at: 0 556 1725
Size: 556 1169
SmaI CCC'GGG
Cuts at: 0 126 146 165 221 231 737 1725
Size: 126 20 19 56 10 506 988
Fragments arranged by size:
988 506 126 56 20 19 10
SmlI C'TyrA_G
Cuts at: 0 1237 1725
Size: 1237 488
SrfI GCCC'GGGC
Cuts at: 0 126 1725
Size: 126 1599
StuI AGG'CCT
Cuts at: 0 511 1725
Size: 511 1214
StyI C'CwwG_G
Cuts at: 0 482 506 1725
Size: 482 24 1219
Fragments arranged by size:
1219 482 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 937 1725
Size: 937 788
TatI w'GTAC_w
Cuts at: 0 59 1181 1241 1725
Size: 59 1122 60 484
Fragments arranged by size:
1122 484 60 59
Tth111I GACn'n_nGTC
Cuts at: 0 412 700 1129 1330 1725
Size: 412 288 429 201 395
Fragments arranged by size:
429 412 395 288 201
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 489 1725
Size: 489 1236
XhoI C'TCGA_G
Cuts at: 0 1237 1725
Size: 1237 488
XmaI C'CCGG_G
Cuts at: 0 124 144 163 219 229 735 1725
Size: 124 20 19 56 10 506 990
Fragments arranged by size:
990 506 124 56 20 19 10
Enzymes that do cut:
AarI AcuI AfeI AflIII AgeI AlfI AloI AlwNI
ApaI ApoI AvaI BanI BanII BbeI BbsI BcgI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspHI BspMI BsrBI
BsrFI BssSI BstAPI BstYI BtgI BtsI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI HaeII
HincII KasI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV PasI PflMI PfoI PpiI PpuMI
PspOMI PspXI PstI PvuII SacI SapI ScaI SexAI
SfcI SfiI SfoI SgrAI SmaI SmlI SrfI StuI
StyI TaqII TatI Tth111I XcmI XhoI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AhdI AleI ApaLI
AscI AseI AsiSI AvrII BaeI BamHI BbvCI BciVI
BclI BfrBI BmtI BpuEI BsaAI BsaBI BsmBI BspEI
BsrDI BsrGI BssHII BstBI BstEII BstXI BstZ17I Bsu36I
BtgZI ClaI CspCI DraI DraIII DrdI EcoRV FalI
FseI FspI FspAI Hin4I HindIII HpaI KpnI MfeI
NdeI NheI NotI NruI NsiI NspI PacI PciI
PmeI PmlI PshAI PsiI PsrI PvuI RsrII SacII
SalI SanDI SbfI SgfI SnaBI SpeI SphI SspI
SwaI TstI XbaI XmnI ZraI