Restriction map for
pFN21AE1995
(Linear) (Six-base) MAPSORT of: KIEE1995.seq Check: 7841 from: 1 to: 1725
KIEE1995.orf
With 182 enzymes: SgfI *
July 3, 2012 17:06 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1247 1700 1725
Size: 1247 453 25
Fragments arranged by size:
1247 453 25
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 607 850 1725
Size: 607 243 875
Fragments arranged by size:
875 607 243
AfeI AGC'GCT
Cuts at: 0 217 1327 1725
Size: 217 1110 398
Fragments arranged by size:
1110 398 217
AleI CACnn'nnGTG
Cuts at: 0 823 1344 1725
Size: 823 521 381
Fragments arranged by size:
823 521 381
AlwNI CAG_nnn'CTG
Cuts at: 0 882 1623 1725
Size: 882 741 102
Fragments arranged by size:
882 741 102
ApaI G_GGCC'C
Cuts at: 0 287 395 681 688 1147 1725
Size: 287 108 286 7 459 578
Fragments arranged by size:
578 459 287 286 108 7
ApaLI G'TGCA_C
Cuts at: 0 786 912 1725
Size: 786 126 813
Fragments arranged by size:
813 786 126
ApoI r'AATT_y
Cuts at: 0 885 1725
Size: 885 840
AvaI C'yCGr_G
Cuts at: 0 113 146 388 487 563 577 681 1342
Size: 113 33 242 99 76 14 104 661
Cuts at: 1342 1520 1564 1649 1725
Size: 178 44 85 76
Fragments arranged by size:
661 242 178 113 104 99 85 76
76 44 33 14
BanI G'GyrC_C
Cuts at: 0 249 1351 1584 1725
Size: 249 1102 233 141
Fragments arranged by size:
1102 249 233 141
BanII G_rGCy'C
Cuts at: 0 287 395 496 681 688 716 905 1147
Size: 287 108 101 185 7 28 189 242
Cuts at: 1147 1529 1650 1725
Size: 382 121 75
Fragments arranged by size:
382 287 242 189 185 121 108 101
75 28 7
BbeI G_GCGC'C
Cuts at: 0 253 1355 1725
Size: 253 1102 370
Fragments arranged by size:
1102 370 253
BbsI GAAGACnn'nnnn_
Cuts at: 0 1066 1725
Size: 1066 659
BbvCI CC'TCA_GC
Cuts at: 0 45 960 1725
Size: 45 915 765
Fragments arranged by size:
915 765 45
BfrBI ATG'CAT
Cuts at: 0 951 1725
Size: 951 774
BglI GCCn_nnn'nGGC
Cuts at: 0 55 106 390 622 692 1725
Size: 55 51 284 232 70 1033
Fragments arranged by size:
1033 284 232 70 55 51
BglII A'GATC_T
Cuts at: 0 1571 1725
Size: 1571 154
BlpI GC'TnA_GC
Cuts at: 0 899 1124 1528 1725
Size: 899 225 404 197
Fragments arranged by size:
899 404 225 197
Bme1580I G_kGCm'C
Cuts at: 0 287 395 645 681 688 790 916 1147
Size: 287 108 250 36 7 102 126 231
Cuts at: 1147 1725
Size: 578
Fragments arranged by size:
578 287 250 231 126 108 102 36
7
BmgBI CAC'GTC
Cuts at: 0 980 1725
Size: 980 745
BmrI ACTGGGnnnn_n'
Cuts at: 0 1458 1482 1725
Size: 1458 24 243
Fragments arranged by size:
1458 243 24
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 96 573 730 747 949 1092 1359 1645
Size: 96 477 157 17 202 143 267 286
Cuts at: 1645 1725
Size: 80
Fragments arranged by size:
477 286 267 202 157 143 96 80
17
Bpu10I CC'TnA_GC
Cuts at: 0 45 960 1725
Size: 45 915 765
Fragments arranged by size:
915 765 45
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 817 1725
Size: 817 908
BsaI GGTCTCn'nnnn_
Cuts at: 0 505 1546 1725
Size: 505 1041 179
Fragments arranged by size:
1041 505 179
BsaAI yAC'GTr
Cuts at: 0 1203 1383 1725
Size: 1203 180 342
Fragments arranged by size:
1203 342 180
BsaHI Gr'CG_yC
Cuts at: 0 250 304 1352 1725
Size: 250 54 1048 373
Fragments arranged by size:
1048 373 250 54
BsaWI w'CCGG_w
Cuts at: 0 842 1725
Size: 842 883
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 610 640 1663 1693 1725
Size: 610 30 1023 30 32
Fragments arranged by size:
1023 610 32 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 15 379 1725
Size: 15 364 1346
Fragments arranged by size:
1346 364 15
BseYI C'CCAG_C
Cuts at: 0 288 1725
Size: 288 1437
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1297 1725
Size: 1297 428
BsiEI CG_ry'CG
Cuts at: 0 385 452 702 1301 1725
Size: 385 67 250 599 424
Fragments arranged by size:
599 424 385 250 67
BsiHKAI G_wGCw'C
Cuts at: 0 716 790 905 916 937 1208 1725
Size: 716 74 115 11 21 271 517
Fragments arranged by size:
716 517 271 115 74 21 11
BsmBI CGTCTCn'nnnn_
Cuts at: 0 863 1177 1398 1725
Size: 863 314 221 327
Fragments arranged by size:
863 327 314 221
Bsp1286I G_dGCh'C
Cuts at: 0 287 395 496 645 681 688 716 790
Size: 287 108 101 149 36 7 28 74
Cuts at: 790 905 916 937 1147 1208 1529 1650 1725
Size: 115 11 21 210 61 321 121 75
Fragments arranged by size:
321 287 210 149 121 115 108 101
75 74 61 36 28 21 11 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1247 1700 1725
Size: 1247 453 25
Fragments arranged by size:
1247 453 25
BsrBI CCG'CTC
Cuts at: 0 446 1260 1725
Size: 446 814 465
Fragments arranged by size:
814 465 446
BssHII G'CGCG_C
Cuts at: 0 174 1725
Size: 174 1551
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1383 1725
Size: 1383 342
BstYI r'GATC_y
Cuts at: 0 1571 1725
Size: 1571 154
BtgI C'CryG_G
Cuts at: 0 67 279 379 629 1095 1725
Size: 67 212 100 250 466 630
Fragments arranged by size:
630 466 250 212 100 67
DraIII CAC_nnn'GTG
Cuts at: 0 1584 1725
Size: 1584 141
EaeI y'GGCC_r
Cuts at: 0 382 449 533 614 1604 1725
Size: 382 67 84 81 990 121
Fragments arranged by size:
990 382 121 84 81 67
EagI C'GGCC_G
Cuts at: 0 382 449 1725
Size: 382 67 1276
Fragments arranged by size:
1276 382 67
EarI CTCTTCn'nnn_
Cuts at: 0 39 214 721 1432 1725
Size: 39 175 507 711 293
Fragments arranged by size:
711 507 293 175 39
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 415 427 1725
Size: 415 12 1298
Fragments arranged by size:
1298 415 12
EcoICRI GAG'CTC
Cuts at: 0 714 903 1725
Size: 714 189 822
Fragments arranged by size:
822 714 189
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 96 573 607 730 747 850 949 1092
Size: 96 477 34 123 17 103 99 143
Cuts at: 1092 1359 1645 1725
Size: 267 286 80
Fragments arranged by size:
477 286 267 143 123 103 99 96
80 34 17
EcoNI CCTnn'n_nnAGG
Cuts at: 0 346 1725
Size: 346 1379
EcoO109I rG'GnC_Cy
Cuts at: 0 284 319 392 678 685 1143 1144 1725
Size: 284 35 73 286 7 458 1 581
Fragments arranged by size:
581 458 286 284 73 35 7 1
EcoRI G'AATT_C
Cuts at: 0 885 1725
Size: 885 840
HaeII r_GCGC'y
Cuts at: 0 219 253 586 670 1329 1355 1725
Size: 219 34 333 84 659 26 370
Fragments arranged by size:
659 370 333 219 84 34 26
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 172 204 610 642 1444 1476 1663 1695
Size: 172 32 406 32 802 32 187 32
Cuts at: 1695 1725
Size: 30
Fragments arranged by size:
802 406 187 172 32 32 32 32
30
KasI G'GCGC_C
Cuts at: 0 249 1351 1725
Size: 249 1102 374
Fragments arranged by size:
1102 374 249
MscI TGG'CCA
Cuts at: 0 535 1606 1725
Size: 535 1071 119
Fragments arranged by size:
1071 535 119
MslI CAynn'nnrTG
Cuts at: 0 823 1344 1725
Size: 823 521 381
Fragments arranged by size:
823 521 381
MspA1I CmG'CkG
Cuts at: 0 69 171 381 466 478 638 882 1128
Size: 69 102 210 85 12 160 244 246
Cuts at: 1128 1137 1725
Size: 9 588
Fragments arranged by size:
588 246 244 210 160 102 85 69
12 9
NarI GG'CG_CC
Cuts at: 0 250 1352 1725
Size: 250 1102 373
Fragments arranged by size:
1102 373 250
NcoI C'CATG_G
Cuts at: 0 629 1725
Size: 629 1096
NotI GC'GGCC_GC
Cuts at: 0 382 1725
Size: 382 1343
NsiI A_TGCA'T
Cuts at: 0 953 1725
Size: 953 772
NspI r_CATG'y
Cuts at: 0 1311 1725
Size: 1311 414
PasI CC'CwG_GG
Cuts at: 0 1546 1725
Size: 1546 179
PfoI T'CCnGG_A
Cuts at: 0 770 1681 1725
Size: 770 911 44
Fragments arranged by size:
911 770 44
PspOMI G'GGCC_C
Cuts at: 0 283 391 677 684 1143 1725
Size: 283 108 286 7 459 582
Fragments arranged by size:
582 459 286 283 108 7
PspXI vC'TCGA_Gb
Cuts at: 0 1342 1725
Size: 1342 383
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 124 156 1725
Size: 124 32 1569
Fragments arranged by size:
1569 124 32
PstI C_TGCA'G
Cuts at: 0 20 197 1142 1619 1725
Size: 20 177 945 477 106
Fragments arranged by size:
945 477 177 106 20
PvuII CAG'CTG
Cuts at: 0 882 1128 1137 1725
Size: 882 246 9 588
Fragments arranged by size:
882 588 246 9
SacI G_AGCT'C
Cuts at: 0 716 905 1725
Size: 716 189 820
Fragments arranged by size:
820 716 189
SacII CC_GC'GG
Cuts at: 0 70 382 1725
Size: 70 312 1343
Fragments arranged by size:
1343 312 70
SapI GCTCTTCn'nnn_
Cuts at: 0 721 1725
Size: 721 1004
ScaI AGT'ACT
Cuts at: 0 133 1725
Size: 133 1592
SexAI A'CCwGG_T
Cuts at: 0 1404 1580 1725
Size: 1404 176 145
Fragments arranged by size:
1404 176 145
SfcI C'TryA_G
Cuts at: 0 16 193 606 1138 1615 1725
Size: 16 177 413 532 477 110
Fragments arranged by size:
532 477 413 177 110 16
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 390 622 692 1725
Size: 390 232 70 1033
Fragments arranged by size:
1033 390 232 70
SfoI GGC'GCC
Cuts at: 0 251 1353 1725
Size: 251 1102 372
Fragments arranged by size:
1102 372 251
SmaI CCC'GGG
Cuts at: 0 115 148 390 489 565 683 1522 1651
Size: 115 33 242 99 76 118 839 129
Cuts at: 1651 1725
Size: 74
Fragments arranged by size:
839 242 129 118 115 99 76 74
33
SmlI C'TyrA_G
Cuts at: 0 796 1342 1725
Size: 796 546 383
Fragments arranged by size:
796 546 383
SrfI GCCC'GGGC
Cuts at: 0 390 1725
Size: 390 1335
StuI AGG'CCT
Cuts at: 0 625 1725
Size: 625 1100
StyI C'CwwG_G
Cuts at: 0 293 347 629 991 1017 1725
Size: 293 54 282 362 26 708
Fragments arranged by size:
708 362 293 282 54 26
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 741 1287 1725
Size: 741 546 438
Fragments arranged by size:
741 546 438
TatI w'GTAC_w
Cuts at: 0 131 1712 1725
Size: 131 1581 13
Fragments arranged by size:
1581 131 13
XhoI C'TCGA_G
Cuts at: 0 1342 1725
Size: 1342 383
XmaI C'CCGG_G
Cuts at: 0 113 146 388 487 563 681 1520 1649
Size: 113 33 242 99 76 118 839 129
Cuts at: 1649 1725
Size: 76
Fragments arranged by size:
839 242 129 118 113 99 76 76
33
Enzymes that do cut:
AarI AcuI AfeI AleI AlwNI ApaI ApaLI ApoI
AvaI BanI BanII BbeI BbsI BbvCI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI
BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BssHII BstXI
BstYI BtgI DraIII EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI HaeII Hin4I KasI MscI
MslI MspA1I NarI NcoI NotI NsiI NspI PasI
PfoI PspOMI PspXI PsrI PstI PvuII SacI SacII
SapI ScaI SexAI SfcI SfiI SfoI SmaI SmlI
SrfI StuI StyI TaqII TatI XhoI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AflIII AgeI AhdI
AlfI AloI AscI AseI AsiSI AvrII BaeI BamHI
BcgI BciVI BclI BmtI BplI BsaBI BsiWI BsmI
BspEI BspHI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstEII BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI
DrdI EcoRV FalI FseI FspI FspAI HincII HindIII
HpaI KpnI MfeI MluI MmeI NaeI NdeI NgoMIV
NheI NruI PacI PciI PflMI PmeI PmlI PpiI
PpuMI PshAI PsiI PvuI RsrII SalI SanDI SbfI
SgfI SgrAI SnaBI SpeI SphI SspI SwaI TstI
Tth111I XbaI XcmI XmnI ZraI