Restriction map for
pFN21AE1994
(Linear) (Six-base) MAPSORT of: KIEE1994.seq Check: 6953 from: 1 to: 1722
KIEE1994.orf
With 182 enzymes: SgfI *
July 15, 2010 18:52 ..
AccI GT'mk_AC
Cuts at: 0 155 1722
Size: 155 1567
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1276 1722
Size: 1276 446
AflIII A'CryG_T
Cuts at: 0 644 1722
Size: 644 1078
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 753 1722
Size: 753 969
AlwNI CAG_nnn'CTG
Cuts at: 0 822 1401 1642 1722
Size: 822 579 241 80
Fragments arranged by size:
822 579 241 80
ApaI G_GGCC'C
Cuts at: 0 1082 1722
Size: 1082 640
ApoI r'AATT_y
Cuts at: 0 85 1722
Size: 85 1637
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1453 1486 1722
Size: 1453 33 236
Fragments arranged by size:
1453 236 33
BamHI G'GATC_C
Cuts at: 0 31 1722
Size: 31 1691
BanI G'GyrC_C
Cuts at: 0 139 595 1722
Size: 139 456 1127
Fragments arranged by size:
1127 456 139
BanII G_rGCy'C
Cuts at: 0 272 506 945 1082 1636 1722
Size: 272 234 439 137 554 86
Fragments arranged by size:
554 439 272 234 137 86
BbsI GAAGACnn'nnnn_
Cuts at: 0 476 664 1722
Size: 476 188 1058
Fragments arranged by size:
1058 476 188
BbvCI CC'TCA_GC
Cuts at: 0 865 1722
Size: 865 857
BfrBI ATG'CAT
Cuts at: 0 1217 1722
Size: 1217 505
BglI GCCn_nnn'nGGC
Cuts at: 0 1138 1722
Size: 1138 584
Bme1580I G_kGCm'C
Cuts at: 0 142 639 1082 1722
Size: 142 497 443 640
Fragments arranged by size:
640 497 443 142
BmgBI CAC'GTC
Cuts at: 0 119 843 1722
Size: 119 724 879
Fragments arranged by size:
879 724 119
BmrI ACTGGGnnnn_n'
Cuts at: 0 201 1256 1722
Size: 201 1055 466
Fragments arranged by size:
1055 466 201
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 78 1368 1722
Size: 78 1290 354
Fragments arranged by size:
1290 354 78
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 58 490 988 1618 1678 1722
Size: 58 432 498 630 60 44
Fragments arranged by size:
630 498 432 60 58 44
Bpu10I CC'TnA_GC
Cuts at: 0 745 865 1722
Size: 745 120 857
Fragments arranged by size:
857 745 120
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 346 1160 1722
Size: 346 814 562
Fragments arranged by size:
814 562 346
BsaI GGTCTCn'nnnn_
Cuts at: 0 1639 1722
Size: 1639 83
BsaAI yAC'GTr
Cuts at: 0 81 1722
Size: 81 1641
BsaWI w'CCGG_w
Cuts at: 0 671 694 1185 1722
Size: 671 23 491 537
Fragments arranged by size:
671 537 491 23
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 676 706 1722
Size: 676 30 1016
Fragments arranged by size:
1016 676 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1483 1488 1722
Size: 1483 5 234
Fragments arranged by size:
1483 234 5
BseYI C'CCAG_C
Cuts at: 0 874 887 1635 1722
Size: 874 13 748 87
Fragments arranged by size:
874 748 87 13
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 461 758 1722
Size: 461 297 964
Fragments arranged by size:
964 461 297
BsiHKAI G_wGCw'C
Cuts at: 0 272 506 842 945 1722
Size: 272 234 336 103 777
Fragments arranged by size:
777 336 272 234 103
BsiWI C'GTAC_G
Cuts at: 0 176 1722
Size: 176 1546
BsmBI CGTCTCn'nnnn_
Cuts at: 0 244 1722
Size: 244 1478
Bsp1286I G_dGCh'C
Cuts at: 0 142 272 506 639 842 945 1082 1636
Size: 142 130 234 133 203 103 137 554
Cuts at: 1636 1722
Size: 86
Fragments arranged by size:
554 234 203 142 137 133 130 103
86
BspEI T'CCGG_A
Cuts at: 0 694 1185 1722
Size: 694 491 537
Fragments arranged by size:
694 537 491
BspHI T'CATG_A
Cuts at: 0 602 1722
Size: 602 1120
BsrDI GCAATG_nn'
Cuts at: 0 1387 1722
Size: 1387 335
BsrFI r'CCGG_y
Cuts at: 0 308 455 1722
Size: 308 147 1267
Fragments arranged by size:
1267 308 147
BstEII G'GTnAC_C
Cuts at: 0 285 1101 1347 1722
Size: 285 816 246 375
Fragments arranged by size:
816 375 285 246
BstXI CCAn_nnnn'nTGG
Cuts at: 0 882 1586 1674 1722
Size: 882 704 88 48
Fragments arranged by size:
882 704 88 48
BstYI r'GATC_y
Cuts at: 0 31 66 1031 1722
Size: 31 35 965 691
Fragments arranged by size:
965 691 35 31
Bsu36I CC'TnA_GG
Cuts at: 0 53 1070 1097 1273 1722
Size: 53 1017 27 176 449
Fragments arranged by size:
1017 449 176 53 27
BtgI C'CryG_G
Cuts at: 0 713 1722
Size: 713 1009
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 461 1722
Size: 461 1261
BtsI GCAGTG_nn'
Cuts at: 0 661 1717 1722
Size: 661 1056 5
Fragments arranged by size:
1056 661 5
ClaI AT'CG_AT
Cuts at: 0 400 1722
Size: 400 1322
EaeI y'GGCC_r
Cuts at: 0 450 1722
Size: 450 1272
EcoICRI GAG'CTC
Cuts at: 0 270 504 943 1722
Size: 270 234 439 779
Fragments arranged by size:
779 439 270 234
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 58 490 988 1276 1618 1678 1722
Size: 58 432 498 288 342 60 44
Fragments arranged by size:
498 432 342 288 60 58 44
EcoO109I rG'GnC_Cy
Cuts at: 0 57 1079 1590 1722
Size: 57 1022 511 132
Fragments arranged by size:
1022 511 132 57
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1306 1722
Size: 1306 416
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 402 434 1408 1440 1722
Size: 402 32 974 32 282
Fragments arranged by size:
974 402 282 32 32
HincII GTy'rAC
Cuts at: 0 513 1722
Size: 513 1209
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1037 1722
Size: 1037 685
MscI TGG'CCA
Cuts at: 0 452 1722
Size: 452 1270
MslI CAynn'nnrTG
Cuts at: 0 322 601 1722
Size: 322 279 1121
Fragments arranged by size:
1121 322 279
MspA1I CmG'CkG
Cuts at: 0 128 216 292 822 869 951 1193 1309
Size: 128 88 76 530 47 82 242 116
Cuts at: 1309 1722
Size: 413
Fragments arranged by size:
530 413 242 128 116 88 82 76
47
NcoI C'CATG_G
Cuts at: 0 713 1722
Size: 713 1009
NsiI A_TGCA'T
Cuts at: 0 1219 1722
Size: 1219 503
NspI r_CATG'y
Cuts at: 0 648 1323 1722
Size: 648 675 399
Fragments arranged by size:
675 648 399
PasI CC'CwG_GG
Cuts at: 0 926 1401 1586 1722
Size: 926 475 185 136
Fragments arranged by size:
926 475 185 136
PciI A'CATG_T
Cuts at: 0 644 1722
Size: 644 1078
PflMI CCAn_nnn'nTGG
Cuts at: 0 1309 1401 1722
Size: 1309 92 321
Fragments arranged by size:
1309 321 92
PfoI T'CCnGG_A
Cuts at: 0 833 1722
Size: 833 889
PmlI CAC'GTG
Cuts at: 0 81 1722
Size: 81 1641
PpuMI rG'GwC_Cy
Cuts at: 0 57 1722
Size: 57 1665
PshAI GACnn'nnGTC
Cuts at: 0 1574 1722
Size: 1574 148
PspOMI G'GGCC_C
Cuts at: 0 1078 1722
Size: 1078 644
PstI C_TGCA'G
Cuts at: 0 350 939 1343 1722
Size: 350 589 404 379
Fragments arranged by size:
589 404 379 350
PvuII CAG'CTG
Cuts at: 0 1193 1309 1722
Size: 1193 116 413
Fragments arranged by size:
1193 413 116
SacI G_AGCT'C
Cuts at: 0 272 506 945 1722
Size: 272 234 439 777
Fragments arranged by size:
777 439 272 234
SbfI CC_TGCA'GG
Cuts at: 0 939 1722
Size: 939 783
ScaI AGT'ACT
Cuts at: 0 734 901 1722
Size: 734 167 821
Fragments arranged by size:
821 734 167
SfcI C'TryA_G
Cuts at: 0 156 346 750 935 1339 1722
Size: 156 190 404 185 404 383
Fragments arranged by size:
404 404 383 190 185 156
SmlI C'TyrA_G
Cuts at: 0 361 1175 1722
Size: 361 814 547
Fragments arranged by size:
814 547 361
StuI AGG'CCT
Cuts at: 0 1278 1694 1722
Size: 1278 416 28
Fragments arranged by size:
1278 416 28
StyI C'CwwG_G
Cuts at: 0 534 591 626 713 1355 1572 1616 1722
Size: 534 57 35 87 642 217 44 106
Fragments arranged by size:
642 534 217 106 87 57 44 35
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 183 1722
Size: 183 1539
TatI w'GTAC_w
Cuts at: 0 732 899 1722
Size: 732 167 823
Fragments arranged by size:
823 732 167
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1510 1542 1722
Size: 1510 32 180
Fragments arranged by size:
1510 180 32
Tth111I GACn'n_nGTC
Cuts at: 0 151 1722
Size: 151 1571
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 24 532 674 1722
Size: 24 508 142 1048
Fragments arranged by size:
1048 508 142 24
Enzymes that do cut:
AccI AcuI AflIII AlfI AlwNI ApaI ApoI BaeI
BamHI BanI BanII BbsI BbvCI BfrBI BglI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsiWI BsmBI
Bsp1286I BspEI BspHI BsrDI BsrFI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI EaeI EcoICRI Eco57MI
EcoO109I FalI Hin4I HincII MmeI MscI MslI MspA1I
NcoI NsiI NspI PasI PciI PflMI PfoI PmlI
PpuMI PshAI PspOMI PstI PvuII SacI SbfI ScaI
SfcI SmlI StuI StyI TaqII TatI TstI Tth111I
XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AloI ApaLI AscI AseI AsiSI AvaI AvrII
BbeI BcgI BciVI BclI BglII BlpI BmtI BsaBI
BsaHI BsiEI BsmI BspMI BsrBI BsrGI BssHII BssSI
BstAPI BstBI BstZ17I CspCI DraI DraIII DrdI EagI
EarI EciI EcoNI EcoRI EcoRV FseI FspI FspAI
HaeII HindIII HpaI KasI KpnI MfeI MluI NaeI
NarI NdeI NgoMIV NheI NotI NruI PacI PmeI
PpiI PsiI PspXI PsrI PvuI RsrII SacII SalI
SanDI SapI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI SwaI XbaI XhoI
XmaI XmnI ZraI