Restriction map for
pFN21AE1991
(Linear) (Six-base) MAPSORT of: KIEE1991.seq Check: 9229 from: 1 to: 1707
KIEE1991.orf
With 182 enzymes: SgfI *
July 15, 2010 18:52 ..
AccI GT'mk_AC
Cuts at: 0 1136 1333 1707
Size: 1136 197 374
Fragments arranged by size:
1136 374 197
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 281 1071 1151 1550 1707
Size: 281 790 80 399 157
Fragments arranged by size:
790 399 281 157 80
AflII C'TTAA_G
Cuts at: 0 982 1707
Size: 982 725
AflIII A'CryG_T
Cuts at: 0 506 802 1707
Size: 506 296 905
Fragments arranged by size:
905 506 296
AleI CACnn'nnGTG
Cuts at: 0 243 1707
Size: 243 1464
ApaI G_GGCC'C
Cuts at: 0 1352 1707
Size: 1352 355
ApoI r'AATT_y
Cuts at: 0 514 1042 1612 1624 1684 1707
Size: 514 528 570 12 60 23
Fragments arranged by size:
570 528 514 60 23 12
AseI AT'TA_AT
Cuts at: 0 935 1707
Size: 935 772
AvaI C'yCGr_G
Cuts at: 0 823 1707
Size: 823 884
BanII G_rGCy'C
Cuts at: 0 1352 1459 1707
Size: 1352 107 248
Fragments arranged by size:
1352 248 107
BciVI GTATCCnnnnn_n'
Cuts at: 0 48 343 802 1707
Size: 48 295 459 905
Fragments arranged by size:
905 459 295 48
BlpI GC'TnA_GC
Cuts at: 0 473 1707
Size: 473 1234
Bme1580I G_kGCm'C
Cuts at: 0 1352 1707
Size: 1352 355
BmgBI CAC'GTC
Cuts at: 0 507 1707
Size: 507 1200
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1391 1435 1707
Size: 1391 44 272
Fragments arranged by size:
1391 272 44
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1009 1707
Size: 1009 698
BsaBI GATnn'nnATC
Cuts at: 0 710 1308 1707
Size: 710 598 399
Fragments arranged by size:
710 598 399
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 860 890 1220 1250 1707
Size: 860 30 330 30 457
Fragments arranged by size:
860 457 330 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 398 623 1707
Size: 398 225 1084
Fragments arranged by size:
1084 398 225
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1125 1707
Size: 1125 582
BsmBI CGTCTCn'nnnn_
Cuts at: 0 497 1707
Size: 497 1210
Bsp1286I G_dGCh'C
Cuts at: 0 1352 1459 1707
Size: 1352 107 248
Fragments arranged by size:
1352 248 107
BsrDI GCAATG_nn'
Cuts at: 0 911 1707
Size: 911 796
BsrGI T'GTAC_A
Cuts at: 0 327 1707
Size: 327 1380
BstAPI GCAn_nnn'nTGC
Cuts at: 0 212 1707
Size: 212 1495
BstEII G'GTnAC_C
Cuts at: 0 230 581 1707
Size: 230 351 1126
Fragments arranged by size:
1126 351 230
BstYI r'GATC_y
Cuts at: 0 1512 1707
Size: 1512 195
BtgI C'CryG_G
Cuts at: 0 87 1707
Size: 87 1620
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1654 1707
Size: 1654 53
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 559 594 768 803 1707
Size: 559 35 174 35 904
Fragments arranged by size:
904 559 174 35 35
DraIII CAC_nnn'GTG
Cuts at: 0 67 1324 1707
Size: 67 1257 383
Fragments arranged by size:
1257 383 67
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 281 1071 1151 1391 1435 1550 1707
Size: 281 790 80 240 44 115 157
Fragments arranged by size:
790 281 240 157 115 80 44
EcoO109I rG'GnC_Cy
Cuts at: 0 1349 1707
Size: 1349 358
EcoRI G'AATT_C
Cuts at: 0 1612 1707
Size: 1612 95
EcoRV GAT'ATC
Cuts at: 0 1646 1707
Size: 1646 61
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 79 1707
Size: 79 1628
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 860 892 1366 1398 1707
Size: 860 32 474 32 309
Fragments arranged by size:
860 474 309 32 32
HincII GTy'rAC
Cuts at: 0 1415 1707
Size: 1415 292
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 480 525 619 1707
Size: 480 45 94 1088
Fragments arranged by size:
1088 480 94 45
MslI CAynn'nnrTG
Cuts at: 0 243 1503 1707
Size: 243 1260 204
Fragments arranged by size:
1260 243 204
NcoI C'CATG_G
Cuts at: 0 87 1707
Size: 87 1620
NdeI CA'TA_TG
Cuts at: 0 415 1707
Size: 415 1292
NspI r_CATG'y
Cuts at: 0 215 602 806 1475 1707
Size: 215 387 204 669 232
Fragments arranged by size:
669 387 232 215 204
PciI A'CATG_T
Cuts at: 0 802 1707
Size: 802 905
PsiI TTA'TAA
Cuts at: 0 116 538 724 1707
Size: 116 422 186 983
Fragments arranged by size:
983 422 186 116
PspOMI G'GGCC_C
Cuts at: 0 1348 1707
Size: 1348 359
PspXI vC'TCGA_Gb
Cuts at: 0 823 1707
Size: 823 884
PstI C_TGCA'G
Cuts at: 0 526 1707
Size: 526 1181
SfcI C'TryA_G
Cuts at: 0 137 522 1707
Size: 137 385 1185
Fragments arranged by size:
1185 385 137
SmlI C'TyrA_G
Cuts at: 0 823 982 1024 1707
Size: 823 159 42 683
Fragments arranged by size:
823 683 159 42
StyI C'CwwG_G
Cuts at: 0 87 1707
Size: 87 1620
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 566 1707
Size: 566 1141
TatI w'GTAC_w
Cuts at: 0 327 1707
Size: 327 1380
XbaI T'CTAG_A
Cuts at: 0 1057 1707
Size: 1057 650
XhoI C'TCGA_G
Cuts at: 0 823 1707
Size: 823 884
XmnI GAAnn'nnTTC
Cuts at: 0 258 1707
Size: 258 1449
Enzymes that do cut:
AccI AcuI AflII AflIII AleI ApaI ApoI AseI
AvaI BanII BciVI BlpI Bme1580I BmgBI BpmI BpuEI
BsaBI BsaXI BseRI BsgI BsmBI Bsp1286I BsrDI BsrGI
BstAPI BstEII BstYI BtgI BtgZI CspCI DraIII Eco57MI
EcoO109I EcoRI EcoRV FalI Hin4I HincII MmeI MslI
NcoI NdeI NspI PciI PsiI PspOMI PspXI PstI
SfcI SmlI StyI TaqII TatI XbaI XhoI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AgeI AhdI AlfI
AloI AlwNI ApaLI AscI AsiSI AvrII BaeI BamHI
BanI BbeI BbsI BbvCI BcgI BclI BfrBI BglI
BglII BmrI BmtI BplI Bpu10I BsaI BsaAI BsaHI
BsaWI BseYI BsiEI BsiHKAI BsiWI BsmI BspEI BspHI
BspMI BsrBI BsrFI BssHII BssSI BstBI BstXI BstZ17I
Bsu36I BtsI ClaI DraI DrdI EaeI EagI EarI
EciI EcoICRI EcoNI FseI FspI FspAI HaeII HindIII
HpaI KasI KpnI MfeI MluI MscI MspA1I NaeI
NarI NgoMIV NheI NotI NruI NsiI PacI PasI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsrI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI StuI SwaI TstI
Tth111I XcmI XmaI ZraI