Restriction map for
pFN21AE1990
(Linear) (Six-base) MAPSORT of: KIEE1990.seq Check: 2994 from: 1 to: 1701
KIEE1990.orf
With 182 enzymes: SgfI *
July 15, 2010 18:52 ..
AccI GT'mk_AC
Cuts at: 0 1031 1701
Size: 1031 670
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 274 463 658 1701
Size: 274 189 195 1043
Fragments arranged by size:
1043 274 195 189
AfeI AGC'GCT
Cuts at: 0 304 1701
Size: 304 1397
AflIII A'CryG_T
Cuts at: 0 458 1701
Size: 458 1243
AlwNI CAG_nnn'CTG
Cuts at: 0 390 1275 1317 1701
Size: 390 885 42 384
Fragments arranged by size:
885 390 384 42
ApaI G_GGCC'C
Cuts at: 0 89 195 1542 1616 1701
Size: 89 106 1347 74 85
Fragments arranged by size:
1347 106 89 85 74
ApoI r'AATT_y
Cuts at: 0 1588 1701
Size: 1588 113
AvaI C'yCGr_G
Cuts at: 0 643 1701
Size: 643 1058
BamHI G'GATC_C
Cuts at: 0 1669 1701
Size: 1669 32
BanI G'GyrC_C
Cuts at: 0 1455 1701
Size: 1455 246
BanII G_rGCy'C
Cuts at: 0 89 195 397 449 787 1234 1300 1542
Size: 89 106 202 52 338 447 66 242
Cuts at: 1542 1616 1701
Size: 74 85
Fragments arranged by size:
447 338 242 202 106 89 85 74
66 52
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 493 527 1701
Size: 493 34 1174
Fragments arranged by size:
1174 493 34
BglI GCCn_nnn'nGGC
Cuts at: 0 190 943 1701
Size: 190 753 758
Fragments arranged by size:
758 753 190
BglII A'GATC_T
Cuts at: 0 482 1346 1701
Size: 482 864 355
Fragments arranged by size:
864 482 355
BlpI GC'TnA_GC
Cuts at: 0 70 391 1701
Size: 70 321 1310
Fragments arranged by size:
1310 321 70
Bme1580I G_kGCm'C
Cuts at: 0 89 195 471 1460 1542 1616 1701
Size: 89 106 276 989 82 74 85
Fragments arranged by size:
989 276 106 89 85 82 74
BmrI ACTGGGnnnn_n'
Cuts at: 0 134 391 1433 1454 1701
Size: 134 257 1042 21 247
Fragments arranged by size:
1042 257 247 134 21
BmtI G_CTAG'C
Cuts at: 0 343 556 1701
Size: 343 213 1145
Fragments arranged by size:
1145 343 213
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1153 1168 1234 1412 1701
Size: 1153 15 66 178 289
Fragments arranged by size:
1153 289 178 66 15
Bpu10I CC'TnA_GC
Cuts at: 0 344 1701
Size: 344 1357
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1248 1701
Size: 1248 453
BsaI GGTCTCn'nnnn_
Cuts at: 0 1621 1701
Size: 1621 80
BsaHI Gr'CG_yC
Cuts at: 0 26 570 614 1701
Size: 26 544 44 1087
Fragments arranged by size:
1087 544 44 26
BsaWI w'CCGG_w
Cuts at: 0 854 1701
Size: 854 847
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 361 391 1701
Size: 361 30 1310
Fragments arranged by size:
1310 361 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 184 445 582 685 786 1639 1701
Size: 184 261 137 103 101 853 62
Fragments arranged by size:
853 261 184 137 103 101 62
BseYI C'CCAG_C
Cuts at: 0 148 188 225 332 660 741 1615 1655
Size: 148 40 37 107 328 81 874 40
Cuts at: 1655 1701
Size: 46
Fragments arranged by size:
874 328 148 107 81 46 40 40
37
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1062 1234 1546 1701
Size: 1062 172 312 155
Fragments arranged by size:
1062 312 172 155
BsiEI CG_ry'CG
Cuts at: 0 411 454 1701
Size: 411 43 1247
Fragments arranged by size:
1247 411 43
BsiHKAI G_wGCw'C
Cuts at: 0 874 1300 1701
Size: 874 426 401
Fragments arranged by size:
874 426 401
Bsp1286I G_dGCh'C
Cuts at: 0 89 195 397 449 471 787 874 1234
Size: 89 106 202 52 22 316 87 360
Cuts at: 1234 1300 1460 1542 1616 1701
Size: 66 160 82 74 85
Fragments arranged by size:
360 316 202 160 106 89 87 85
82 74 66 52 22
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 929 1701
Size: 929 772
BsrBI CCG'CTC
Cuts at: 0 207 1701
Size: 207 1494
BsrDI GCAATG_nn'
Cuts at: 0 1050 1701
Size: 1050 651
BsrFI r'CCGG_y
Cuts at: 0 893 1253 1701
Size: 893 360 448
Fragments arranged by size:
893 448 360
BssHII G'CGCG_C
Cuts at: 0 514 1701
Size: 514 1187
BssSI C'ACGA_G
Cuts at: 0 618 1701
Size: 618 1083
BstXI CCAn_nnnn'nTGG
Cuts at: 0 21 1446 1701
Size: 21 1425 255
Fragments arranged by size:
1425 255 21
BstYI r'GATC_y
Cuts at: 0 314 482 989 1278 1346 1669 1701
Size: 314 168 507 289 68 323 32
Fragments arranged by size:
507 323 314 289 168 68 32
BtgI C'CryG_G
Cuts at: 0 108 1701
Size: 108 1593
DrdI GACnn_nn'nnGTC
Cuts at: 0 1027 1701
Size: 1027 674
EaeI y'GGCC_r
Cuts at: 0 182 320 440 451 895 950 1255 1701
Size: 182 138 120 11 444 55 305 446
Fragments arranged by size:
446 444 305 182 138 120 55 11
EagI C'GGCC_G
Cuts at: 0 451 1701
Size: 451 1250
EarI CTCTTCn'nnn_
Cuts at: 0 853 1337 1701
Size: 853 484 364
Fragments arranged by size:
853 484 364
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 721 789 1105 1701
Size: 721 68 316 596
Fragments arranged by size:
721 596 316 68
EcoICRI GAG'CTC
Cuts at: 0 1298 1701
Size: 1298 403
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 274 463 658 1153 1168 1234 1412 1701
Size: 274 189 195 495 15 66 178 289
Fragments arranged by size:
495 289 274 195 189 178 66 15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 256 1069 1701
Size: 256 813 632
Fragments arranged by size:
813 632 256
EcoO109I rG'GnC_Cy
Cuts at: 0 85 86 192 219 700 1056 1538 1554
Size: 85 1 106 27 481 356 482 16
Cuts at: 1554 1572 1612 1701
Size: 18 40 89
Fragments arranged by size:
482 481 356 106 89 85 40 27
18 16 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1242 1701
Size: 1242 459
HaeII r_GCGC'y
Cuts at: 0 306 391 1147 1201 1701
Size: 306 85 756 54 500
Fragments arranged by size:
756 500 306 85 54
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 361 393 947 979 1701
Size: 361 32 554 32 722
Fragments arranged by size:
722 554 361 32 32
MscI TGG'CCA
Cuts at: 0 184 952 1701
Size: 184 768 749
Fragments arranged by size:
768 749 184
MspA1I CmG'CkG
Cuts at: 0 110 188 225 534 660 684 1535 1599
Size: 110 78 37 309 126 24 851 64
Cuts at: 1599 1701
Size: 102
Fragments arranged by size:
851 309 126 110 102 78 64 37
24
NaeI GCC'GGC
Cuts at: 0 895 1701
Size: 895 806
NgoMIV G'CCGG_C
Cuts at: 0 893 1701
Size: 893 808
NheI G'CTAG_C
Cuts at: 0 339 552 1701
Size: 339 213 1149
Fragments arranged by size:
1149 339 213
NspI r_CATG'y
Cuts at: 0 462 981 1701
Size: 462 519 720
Fragments arranged by size:
720 519 462
PasI CC'CwG_GG
Cuts at: 0 90 1701
Size: 90 1611
PciI A'CATG_T
Cuts at: 0 458 1701
Size: 458 1243
PflMI CCAn_nnn'nTGG
Cuts at: 0 1317 1701
Size: 1317 384
PfoI T'CCnGG_A
Cuts at: 0 1064 1701
Size: 1064 637
PpuMI rG'GwC_Cy
Cuts at: 0 219 700 1572 1701
Size: 219 481 872 129
Fragments arranged by size:
872 481 219 129
PspOMI G'GGCC_C
Cuts at: 0 85 191 1538 1612 1701
Size: 85 106 1347 74 89
Fragments arranged by size:
1347 106 89 85 74
PstI C_TGCA'G
Cuts at: 0 1193 1205 1598 1701
Size: 1193 12 393 103
Fragments arranged by size:
1193 393 103 12
PvuII CAG'CTG
Cuts at: 0 188 684 1599 1701
Size: 188 496 915 102
Fragments arranged by size:
915 496 188 102
SacI G_AGCT'C
Cuts at: 0 1300 1701
Size: 1300 401
SacII CC_GC'GG
Cuts at: 0 111 1701
Size: 111 1590
SapI GCTCTTCn'nnn_
Cuts at: 0 853 1701
Size: 853 848
ScaI AGT'ACT
Cuts at: 0 907 1701
Size: 907 794
SexAI A'CCwGG_T
Cuts at: 0 1034 1574 1701
Size: 1034 540 127
Fragments arranged by size:
1034 540 127
SfcI C'TryA_G
Cuts at: 0 1189 1201 1594 1701
Size: 1189 12 393 107
Fragments arranged by size:
1189 393 107 12
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 190 1701
Size: 190 1511
SmaI CCC'GGG
Cuts at: 0 645 1701
Size: 645 1056
SmlI C'TyrA_G
Cuts at: 0 1227 1701
Size: 1227 474
StuI AGG'CCT
Cuts at: 0 295 418 1007 1502 1701
Size: 295 123 589 495 199
Fragments arranged by size:
589 495 295 199 123
StyI C'CwwG_G
Cuts at: 0 813 864 941 1608 1701
Size: 813 51 77 667 93
Fragments arranged by size:
813 667 93 77 51
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 652 1432 1701
Size: 652 780 269
Fragments arranged by size:
780 652 269
TatI w'GTAC_w
Cuts at: 0 905 1701
Size: 905 796
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 39 1701
Size: 39 1662
XmaI C'CCGG_G
Cuts at: 0 643 1701
Size: 643 1058
XmnI GAAnn'nnTTC
Cuts at: 0 262 1701
Size: 262 1439
Enzymes that do cut:
AccI AcuI AfeI AflIII AlwNI ApaI ApoI AvaI
BamHI BanI BanII BcgI BglI BglII BlpI Bme1580I
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstXI BstYI BtgI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I FalI HaeII Hin4I MscI MspA1I NaeI NgoMIV
NheI NspI PasI PciI PflMI PfoI PpuMI PspOMI
PstI PvuII SacI SacII SapI ScaI SexAI SfcI
SfiI SmaI SmlI StuI StyI TaqII TatI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AgeI AhdI AleI
AlfI AloI ApaLI AscI AseI AsiSI AvrII BaeI
BbeI BbsI BbvCI BciVI BclI BfrBI BmgBI BplI
BsaAI BsaBI BsiWI BsmI BsmBI BspEI BspHI BsrGI
BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI BtsI ClaI
CspCI DraI DraIII EcoRI EcoRV FseI FspI FspAI
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MslI NarI NcoI NdeI NotI NruI NsiI PacI
PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI
RsrII SalI SanDI SbfI SfoI SgfI SgrAI SnaBI
SpeI SphI SrfI SspI SwaI TstI Tth111I XbaI
XhoI ZraI