Restriction map for
pFN21AE1988
(Linear) (Six-base) MAPSORT of: KIEE1988.seq Check: 609 from: 1 to: 1692
KIEE1988.orf
With 182 enzymes: SgfI *
July 15, 2010 18:52 ..
AccI GT'mk_AC
Cuts at: 0 1133 1692
Size: 1133 559
AleI CACnn'nnGTG
Cuts at: 0 61 1692
Size: 61 1631
AlwNI CAG_nnn'CTG
Cuts at: 0 621 1692
Size: 621 1071
ApoI r'AATT_y
Cuts at: 0 214 665 1276 1692
Size: 214 451 611 416
Fragments arranged by size:
611 451 416 214
AvaI C'yCGr_G
Cuts at: 0 260 503 1692
Size: 260 243 1189
Fragments arranged by size:
1189 260 243
BanI G'GyrC_C
Cuts at: 0 1653 1692
Size: 1653 39
BanII G_rGCy'C
Cuts at: 0 24 1207 1692
Size: 24 1183 485
Fragments arranged by size:
1183 485 24
BbsI GAAGACnn'nnnn_
Cuts at: 0 296 1692
Size: 296 1396
BfrBI ATG'CAT
Cuts at: 0 360 1692
Size: 360 1332
BglII A'GATC_T
Cuts at: 0 465 507 1692
Size: 465 42 1185
Fragments arranged by size:
1185 465 42
Bme1580I G_kGCm'C
Cuts at: 0 1656 1692
Size: 1656 36
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 310 868 903 1692
Size: 310 558 35 789
Fragments arranged by size:
789 558 310 35
Bpu10I CC'TnA_GC
Cuts at: 0 1283 1692
Size: 1283 409
BsaI GGTCTCn'nnnn_
Cuts at: 0 506 1692
Size: 506 1186
BsaHI Gr'CG_yC
Cuts at: 0 1136 1692
Size: 1136 556
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1492 1522 1692
Size: 1492 30 170
Fragments arranged by size:
1492 170 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 359 1003 1093 1500 1616 1649 1664 1692
Size: 359 644 90 407 116 33 15 28
Fragments arranged by size:
644 407 359 116 90 33 28 15
BseYI C'CCAG_C
Cuts at: 0 1576 1692
Size: 1576 116
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 716 863 1023 1692
Size: 151 565 147 160 669
Fragments arranged by size:
669 565 160 151 147
BsiHKAI G_wGCw'C
Cuts at: 0 68 1692
Size: 68 1624
BsmI GAATG_Cn'
Cuts at: 0 1248 1692
Size: 1248 444
Bsp1286I G_dGCh'C
Cuts at: 0 24 68 1207 1656 1692
Size: 24 44 1139 449 36
Fragments arranged by size:
1139 449 44 36 24
BsrBI CCG'CTC
Cuts at: 0 15 1692
Size: 15 1677
BsrDI GCAATG_nn'
Cuts at: 0 434 1692
Size: 434 1258
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1243 1692
Size: 1243 449
BstYI r'GATC_y
Cuts at: 0 465 507 1692
Size: 465 42 1185
Fragments arranged by size:
1185 465 42
BtgI C'CryG_G
Cuts at: 0 5 1692
Size: 5 1687
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 957 1692
Size: 957 735
BtsI GCAGTG_nn'
Cuts at: 0 1557 1692
Size: 1557 135
EaeI y'GGCC_r
Cuts at: 0 2 53 968 1123 1692
Size: 2 51 915 155 569
Fragments arranged by size:
915 569 155 51 2
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 25 137 1692
Size: 25 112 1555
Fragments arranged by size:
1555 112 25
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 310 868 903 1692
Size: 310 558 35 789
Fragments arranged by size:
789 558 310 35
EcoO109I rG'GnC_Cy
Cuts at: 0 762 1059 1374 1692
Size: 762 297 315 318
Fragments arranged by size:
762 318 315 297
EcoRI G'AATT_C
Cuts at: 0 1276 1692
Size: 1276 416
HaeII r_GCGC'y
Cuts at: 0 850 1440 1583 1692
Size: 850 590 143 109
Fragments arranged by size:
850 590 143 109
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 287 319 892 924 1387 1419 1692
Size: 287 32 573 32 463 32 273
Fragments arranged by size:
573 463 287 273 32 32 32
MfeI C'AATT_G
Cuts at: 0 518 1692
Size: 518 1174
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 762 1143 1208 1692
Size: 762 381 65 484
Fragments arranged by size:
762 484 381 65
MscI TGG'CCA
Cuts at: 0 4 55 970 1125 1692
Size: 4 51 915 155 567
Fragments arranged by size:
915 567 155 51 4
MslI CAynn'nnrTG
Cuts at: 0 61 176 355 1692
Size: 61 115 179 1337
Fragments arranged by size:
1337 179 115 61
MspA1I CmG'CkG
Cuts at: 0 561 676 1692
Size: 561 115 1016
Fragments arranged by size:
1016 561 115
NdeI CA'TA_TG
Cuts at: 0 691 1692
Size: 691 1001
NsiI A_TGCA'T
Cuts at: 0 362 1692
Size: 362 1330
NspI r_CATG'y
Cuts at: 0 360 745 1692
Size: 360 385 947
Fragments arranged by size:
947 385 360
PpuMI rG'GwC_Cy
Cuts at: 0 762 1059 1374 1692
Size: 762 297 315 318
Fragments arranged by size:
762 318 315 297
PstI C_TGCA'G
Cuts at: 0 772 1692
Size: 772 920
PvuII CAG'CTG
Cuts at: 0 561 676 1692
Size: 561 115 1016
Fragments arranged by size:
1016 561 115
SfcI C'TryA_G
Cuts at: 0 768 1692
Size: 768 924
SmlI C'TyrA_G
Cuts at: 0 503 1692
Size: 503 1189
StuI AGG'CCT
Cuts at: 0 314 1683 1692
Size: 314 1369 9
Fragments arranged by size:
1369 314 9
StyI C'CwwG_G
Cuts at: 0 1678 1692
Size: 1678 14
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 780 1692
Size: 780 912
TatI w'GTAC_w
Cuts at: 0 134 1692
Size: 134 1558
Tth111I GACn'n_nGTC
Cuts at: 0 760 790 1692
Size: 760 30 902
Fragments arranged by size:
902 760 30
XhoI C'TCGA_G
Cuts at: 0 503 1692
Size: 503 1189
XmnI GAAnn'nnTTC
Cuts at: 0 544 1692
Size: 544 1148
Enzymes that do cut:
AccI AleI AlwNI ApoI AvaI BanI BanII BbsI
BfrBI BglII Bme1580I BpmI Bpu10I BsaI BsaHI BsaXI
BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BsrBI BsrDI
BstAPI BstYI BtgI BtgZI BtsI EaeI EciI Eco57MI
EcoO109I EcoRI HaeII Hin4I MfeI MmeI MscI MslI
MspA1I NdeI NsiI NspI PpuMI PstI PvuII SfcI
SmlI StuI StyI TaqII TatI Tth111I XhoI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AlfI AloI ApaI ApaLI AscI AseI
AsiSI AvrII BaeI BamHI BbeI BbvCI BcgI BciVI
BclI BglI BlpI BmgBI BmrI BmtI BplI BpuEI
BsaAI BsaBI BsaWI BsiEI BsiWI BsmBI BspEI BspHI
BspMI BsrFI BsrGI BssHII BssSI BstBI BstEII BstXI
BstZ17I Bsu36I ClaI CspCI DraI DraIII DrdI EagI
EarI EcoICRI EcoNI EcoRV FalI FseI FspI FspAI
HincII HindIII HpaI KasI KpnI MluI NaeI NarI
NcoI NgoMIV NheI NotI NruI PacI PasI PciI
PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI SwaI TstI
XbaI XcmI XmaI ZraI