Restriction map for
pFN21AE1987
(Linear) (Six-base) MAPSORT of: KIEE1987.seq Check: 4861 from: 1 to: 1686
KIEE1987.orf
With 182 enzymes: SgfI *
July 15, 2010 18:52 ..
AatII G_ACGT'C
Cuts at: 0 1205 1686
Size: 1205 481
AccI GT'mk_AC
Cuts at: 0 705 1686
Size: 705 981
AclI AA'CG_TT
Cuts at: 0 1562 1686
Size: 1562 124
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 793 1686
Size: 793 893
AflIII A'CryG_T
Cuts at: 0 166 1686
Size: 166 1520
AlwNI CAG_nnn'CTG
Cuts at: 0 1501 1542 1686
Size: 1501 41 144
Fragments arranged by size:
1501 144 41
ApaI G_GGCC'C
Cuts at: 0 1276 1339 1686
Size: 1276 63 347
Fragments arranged by size:
1276 347 63
ApoI r'AATT_y
Cuts at: 0 820 1043 1661 1686
Size: 820 223 618 25
Fragments arranged by size:
820 618 223 25
AvaI C'yCGr_G
Cuts at: 0 1187 1344 1686
Size: 1187 157 342
Fragments arranged by size:
1187 342 157
BanI G'GyrC_C
Cuts at: 0 765 1686
Size: 765 921
BanII G_rGCy'C
Cuts at: 0 162 1194 1276 1339 1461 1686
Size: 162 1032 82 63 122 225
Fragments arranged by size:
1032 225 162 122 82 63
BbeI G_GCGC'C
Cuts at: 0 769 1686
Size: 769 917
BbsI GAAGACnn'nnnn_
Cuts at: 0 493 615 1686
Size: 493 122 1071
Fragments arranged by size:
1071 493 122
BfrBI ATG'CAT
Cuts at: 0 21 1686
Size: 21 1665
BglII A'GATC_T
Cuts at: 0 270 1686
Size: 270 1416
Bme1580I G_kGCm'C
Cuts at: 0 1276 1339 1345 1686
Size: 1276 63 6 341
Fragments arranged by size:
1276 341 63 6
BmgBI CAC'GTC
Cuts at: 0 167 1686
Size: 167 1519
BmrI ACTGGGnnnn_n'
Cuts at: 0 270 1489 1611 1686
Size: 270 1219 122 75
Fragments arranged by size:
1219 270 122 75
Bpu10I CC'TnA_GC
Cuts at: 0 852 1686
Size: 852 834
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 261 1686
Size: 261 1425
BsaAI yAC'GTr
Cuts at: 0 1654 1686
Size: 1654 32
BsaHI Gr'CG_yC
Cuts at: 0 766 952 1202 1686
Size: 766 186 250 484
Fragments arranged by size:
766 484 250 186
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1324 1345 1354 1375 1686
Size: 1324 21 9 21 311
Fragments arranged by size:
1324 311 21 21 9
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 793 1686
Size: 793 893
BseYI C'CCAG_C
Cuts at: 0 744 1686
Size: 744 942
BsiHKAI G_wGCw'C
Cuts at: 0 162 1194 1686
Size: 162 1032 492
Fragments arranged by size:
1032 492 162
BsmBI CGTCTCn'nnnn_
Cuts at: 0 994 1522 1686
Size: 994 528 164
Fragments arranged by size:
994 528 164
Bsp1286I G_dGCh'C
Cuts at: 0 162 1194 1276 1339 1345 1461 1686
Size: 162 1032 82 63 6 116 225
Fragments arranged by size:
1032 225 162 116 82 63 6
BstEII G'GTnAC_C
Cuts at: 0 280 1686
Size: 280 1406
BstXI CCAn_nnnn'nTGG
Cuts at: 0 573 1686
Size: 573 1113
BstYI r'GATC_y
Cuts at: 0 270 1623 1686
Size: 270 1353 63
Fragments arranged by size:
1353 270 63
Bsu36I CC'TnA_GG
Cuts at: 0 178 1196 1686
Size: 178 1018 490
Fragments arranged by size:
1018 490 178
BtgI C'CryG_G
Cuts at: 0 1385 1686
Size: 1385 301
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 380 415 1686
Size: 380 35 1271
Fragments arranged by size:
1271 380 35
DraI TTT'AAA
Cuts at: 0 138 1686
Size: 138 1548
EaeI y'GGCC_r
Cuts at: 0 1115 1265 1529 1686
Size: 1115 150 264 157
Fragments arranged by size:
1115 264 157 150
EarI CTCTTCn'nnn_
Cuts at: 0 114 306 1214 1686
Size: 114 192 908 472
Fragments arranged by size:
908 472 192 114
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 605 1021 1686
Size: 605 416 665
Fragments arranged by size:
665 605 416
EcoICRI GAG'CTC
Cuts at: 0 160 1192 1686
Size: 160 1032 494
Fragments arranged by size:
1032 494 160
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 793 1686
Size: 793 893
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1363 1686
Size: 1363 323
EcoO109I rG'GnC_Cy
Cuts at: 0 1272 1273 1286 1335 1686
Size: 1272 1 13 49 351
Fragments arranged by size:
1272 351 49 13 1
EcoRI G'AATT_C
Cuts at: 0 820 1686
Size: 820 866
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1120 1686
Size: 1120 566
HaeII r_GCGC'y
Cuts at: 0 769 1686
Size: 769 917
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 156 188 489 521 1345 1377 1686
Size: 156 32 301 32 824 32 309
Fragments arranged by size:
824 309 301 156 32 32 32
HincII GTy'rAC
Cuts at: 0 1683 1686
Size: 1683 3
HpaI GTT'AAC
Cuts at: 0 1683 1686
Size: 1683 3
KasI G'GCGC_C
Cuts at: 0 765 1686
Size: 765 921
MfeI C'AATT_G
Cuts at: 0 571 1686
Size: 571 1115
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 814 1686
Size: 814 872
MscI TGG'CCA
Cuts at: 0 1267 1686
Size: 1267 419
MslI CAynn'nnrTG
Cuts at: 0 466 1686
Size: 466 1220
MspA1I CmG'CkG
Cuts at: 0 1173 1327 1686
Size: 1173 154 359
Fragments arranged by size:
1173 359 154
NarI GG'CG_CC
Cuts at: 0 766 1686
Size: 766 920
NsiI A_TGCA'T
Cuts at: 0 23 1686
Size: 23 1663
NspI r_CATG'y
Cuts at: 0 25 100 546 744 1686
Size: 25 75 446 198 942
Fragments arranged by size:
942 446 198 75 25
PflMI CCAn_nnn'nTGG
Cuts at: 0 1263 1686
Size: 1263 423
PfoI T'CCnGG_A
Cuts at: 0 1129 1686
Size: 1129 557
PpuMI rG'GwC_Cy
Cuts at: 0 1286 1686
Size: 1286 400
PspOMI G'GGCC_C
Cuts at: 0 1272 1335 1686
Size: 1272 63 351
Fragments arranged by size:
1272 351 63
PvuII CAG'CTG
Cuts at: 0 1173 1686
Size: 1173 513
SacI G_AGCT'C
Cuts at: 0 162 1194 1686
Size: 162 1032 492
Fragments arranged by size:
1032 492 162
ScaI AGT'ACT
Cuts at: 0 303 1686
Size: 303 1383
SfoI GGC'GCC
Cuts at: 0 767 1686
Size: 767 919
SmlI C'TyrA_G
Cuts at: 0 240 1686
Size: 240 1446
SnaBI TAC'GTA
Cuts at: 0 1654 1686
Size: 1654 32
SphI G_CATG'C
Cuts at: 0 25 744 1686
Size: 25 719 942
Fragments arranged by size:
942 719 25
StuI AGG'CCT
Cuts at: 0 1213 1686
Size: 1213 473
StyI C'CwwG_G
Cuts at: 0 581 1214 1686
Size: 581 633 472
Fragments arranged by size:
633 581 472
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 268 1686
Size: 268 1418
TatI w'GTAC_w
Cuts at: 0 169 301 1686
Size: 169 132 1385
Fragments arranged by size:
1385 169 132
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 394 426 1278 1310 1324 1356 1686
Size: 394 32 852 32 14 32 330
Fragments arranged by size:
852 394 330 32 32 32 14
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 939 1686
Size: 939 747
XmnI GAAnn'nnTTC
Cuts at: 0 1043 1686
Size: 1043 643
ZraI GAC'GTC
Cuts at: 0 1203 1686
Size: 1203 483
Enzymes that do cut:
AatII AccI AclI AcuI AflIII AlwNI ApaI ApoI
AvaI BanI BanII BbeI BbsI BfrBI BglII Bme1580I
BmgBI BmrI Bpu10I BpuEI BsaAI BsaHI BsaXI BseRI
BseYI BsiHKAI BsmBI Bsp1286I BstEII BstXI BstYI Bsu36I
BtgI CspCI DraI EaeI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI HaeII Hin4I HincII HpaI
KasI MfeI MmeI MscI MslI MspA1I NarI NsiI
NspI PflMI PfoI PpuMI PspOMI PvuII SacI ScaI
SfoI SmlI SnaBI SphI StuI StyI TaqII TatI
TstI XcmI XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AfeI AflII AgeI AhdI AleI AlfI
AloI ApaLI AscI AseI AsiSI AvrII BaeI BamHI
BbvCI BcgI BciVI BclI BglI BlpI BmtI BplI
BpmI BsaI BsaBI BsaWI BsgI BsiEI BsiWI BsmI
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstZ17I BtgZI BtsI ClaI DraIII
DrdI EagI EcoRV FseI FspI FspAI HindIII KpnI
MluI NaeI NcoI NdeI NgoMIV NheI NotI NruI
PacI PasI PciI PmeI PmlI PpiI PshAI PsiI
PspXI PsrI PstI PvuI RsrII SacII SalI SanDI
SapI SbfI SexAI SfcI SfiI SgfI SgrAI SmaI
SpeI SrfI SspI SwaI Tth111I XbaI XhoI XmaI