Restriction map for
pFN21AE1983
(Linear) (Six-base) MAPSORT of: KIEE1983.seq Check: 1513 from: 1 to: 1677
KIEE1983.orf
With 182 enzymes: SgfI *
July 15, 2010 18:52 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 283 428 1677
Size: 283 145 1249
Fragments arranged by size:
1249 283 145
AfeI AGC'GCT
Cuts at: 0 281 1677
Size: 281 1396
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 607 1677
Size: 607 1070
AlwNI CAG_nnn'CTG
Cuts at: 0 580 1677
Size: 580 1097
ApoI r'AATT_y
Cuts at: 0 1104 1487 1677
Size: 1104 383 190
Fragments arranged by size:
1104 383 190
BanII G_rGCy'C
Cuts at: 0 323 611 1205 1677
Size: 323 288 594 472
Fragments arranged by size:
594 472 323 288
BbsI GAAGACnn'nnnn_
Cuts at: 0 1256 1677
Size: 1256 421
BbvCI CC'TCA_GC
Cuts at: 0 516 1677
Size: 516 1161
BclI T'GATC_A
Cuts at: 0 1097 1392 1677
Size: 1097 295 285
Fragments arranged by size:
1097 295 285
BfrBI ATG'CAT
Cuts at: 0 1642 1677
Size: 1642 35
BlpI GC'TnA_GC
Cuts at: 0 1333 1677
Size: 1333 344
BmrI ACTGGGnnnn_n'
Cuts at: 0 488 1677
Size: 488 1189
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 337 1677
Size: 337 1340
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 304 361 682 759 1381 1677
Size: 304 57 321 77 622 296
Fragments arranged by size:
622 321 304 296 77 57
Bpu10I CC'TnA_GC
Cuts at: 0 516 621 1067 1219 1677
Size: 516 105 446 152 458
Fragments arranged by size:
516 458 446 152 105
BsaI GGTCTCn'nnnn_
Cuts at: 0 165 498 836 956 1449 1677
Size: 165 333 338 120 493 228
Fragments arranged by size:
493 338 333 228 165 120
BsaAI yAC'GTr
Cuts at: 0 1512 1677
Size: 1512 165
BsaHI Gr'CG_yC
Cuts at: 0 101 1677
Size: 101 1576
BsaWI w'CCGG_w
Cuts at: 0 1253 1677
Size: 1253 424
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 478 508 816 846 1677
Size: 478 30 308 30 831
Fragments arranged by size:
831 478 308 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 69 81 201 331 898 1033 1093 1159
Size: 69 12 120 130 567 135 60 66
Cuts at: 1159 1677
Size: 518
Fragments arranged by size:
567 518 135 130 120 69 66 60
12
BseYI C'CCAG_C
Cuts at: 0 61 455 625 685 1677
Size: 61 394 170 60 992
Fragments arranged by size:
992 394 170 61 60
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 874 1677
Size: 874 803
BsiHKAI G_wGCw'C
Cuts at: 0 323 611 1205 1677
Size: 323 288 594 472
Fragments arranged by size:
594 472 323 288
BsmBI CGTCTCn'nnnn_
Cuts at: 0 427 1677
Size: 427 1250
Bsp1286I G_dGCh'C
Cuts at: 0 323 611 1205 1677
Size: 323 288 594 472
Fragments arranged by size:
594 472 323 288
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1353 1677
Size: 1353 324
BsrBI CCG'CTC
Cuts at: 0 23 47 1002 1677
Size: 23 24 955 675
Fragments arranged by size:
955 675 24 23
BsrFI r'CCGG_y
Cuts at: 0 298 854 1226 1677
Size: 298 556 372 451
Fragments arranged by size:
556 451 372 298
BsrGI T'GTAC_A
Cuts at: 0 402 1677
Size: 402 1275
BssSI C'ACGA_G
Cuts at: 0 31 1677
Size: 31 1646
BstAPI GCAn_nnn'nTGC
Cuts at: 0 374 1677
Size: 374 1303
BstBI TT'CG_AA
Cuts at: 0 171 1677
Size: 171 1506
BstXI CCAn_nnnn'nTGG
Cuts at: 0 660 1677
Size: 660 1017
BstYI r'GATC_y
Cuts at: 0 218 667 1295 1677
Size: 218 449 628 382
Fragments arranged by size:
628 449 382 218
BtgI C'CryG_G
Cuts at: 0 95 671 824 845 1183 1349 1677
Size: 95 576 153 21 338 166 328
Fragments arranged by size:
576 338 328 166 153 95 21
BtsI GCAGTG_nn'
Cuts at: 0 694 1177 1677
Size: 694 483 500
Fragments arranged by size:
694 500 483
DrdI GACnn_nn'nnGTC
Cuts at: 0 64 1569 1677
Size: 64 1505 108
Fragments arranged by size:
1505 108 64
EaeI y'GGCC_r
Cuts at: 0 2 83 203 296 473 902 1208 1228
Size: 2 81 120 93 177 429 306 20
Cuts at: 1228 1677
Size: 449
Fragments arranged by size:
449 429 306 177 120 93 81 20
2
EarI CTCTTCn'nnn_
Cuts at: 0 769 778 866 930 1349 1410 1677
Size: 769 9 88 64 419 61 267
Fragments arranged by size:
769 419 267 88 64 61 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 698 1677
Size: 698 979
EcoICRI GAG'CTC
Cuts at: 0 321 609 1203 1677
Size: 321 288 594 474
Fragments arranged by size:
594 474 321 288
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 283 304 361 428 682 759 1381 1677
Size: 283 21 57 67 254 77 622 296
Fragments arranged by size:
622 296 283 254 77 67 57 21
EcoO109I rG'GnC_Cy
Cuts at: 0 391 1677
Size: 391 1286
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 389 1677
Size: 389 1288
HaeII r_GCGC'y
Cuts at: 0 283 901 1153 1677
Size: 283 618 252 524
Fragments arranged by size:
618 524 283 252
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 159 191 478 510 1280 1312 1677
Size: 159 32 287 32 770 32 365
Fragments arranged by size:
770 365 287 159 32 32 32
HindIII A'AGCT_T
Cuts at: 0 1318 1444 1677
Size: 1318 126 233
Fragments arranged by size:
1318 233 126
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 410 1677
Size: 410 1267
MscI TGG'CCA
Cuts at: 0 205 475 904 1677
Size: 205 270 429 773
Fragments arranged by size:
773 429 270 205
MslI CAynn'nnrTG
Cuts at: 0 1188 1677
Size: 1188 489
MspA1I CmG'CkG
Cuts at: 0 97 520 1677
Size: 97 423 1157
Fragments arranged by size:
1157 423 97
NaeI GCC'GGC
Cuts at: 0 300 1677
Size: 300 1377
NgoMIV G'CCGG_C
Cuts at: 0 298 1677
Size: 298 1379
NsiI A_TGCA'T
Cuts at: 0 1644 1677
Size: 1644 33
NspI r_CATG'y
Cuts at: 0 1642 1677
Size: 1642 35
PasI CC'CwG_GG
Cuts at: 0 210 1677
Size: 210 1467
PpuMI rG'GwC_Cy
Cuts at: 0 391 1677
Size: 391 1286
PvuII CAG'CTG
Cuts at: 0 520 1677
Size: 520 1157
SacI G_AGCT'C
Cuts at: 0 323 611 1205 1677
Size: 323 288 594 472
Fragments arranged by size:
594 472 323 288
SacII CC_GC'GG
Cuts at: 0 98 1677
Size: 98 1579
SapI GCTCTTCn'nnn_
Cuts at: 0 1349 1677
Size: 1349 328
SexAI A'CCwGG_T
Cuts at: 0 488 1677
Size: 488 1189
StyI C'CwwG_G
Cuts at: 0 470 716 1677
Size: 470 246 961
Fragments arranged by size:
961 470 246
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 432 729 837 1677
Size: 432 297 108 840
Fragments arranged by size:
840 432 297 108
TatI w'GTAC_w
Cuts at: 0 402 1613 1677
Size: 402 1211 64
Fragments arranged by size:
1211 402 64
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 816 848 1677
Size: 816 32 829
Fragments arranged by size:
829 816 32
Tth111I GACn'n_nGTC
Cuts at: 0 273 490 1677
Size: 273 217 1187
Fragments arranged by size:
1187 273 217
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 622 1677
Size: 622 1055
XmnI GAAnn'nnTTC
Cuts at: 0 1261 1677
Size: 1261 416
Enzymes that do cut:
AcuI AfeI AlfI AlwNI ApoI BanII BbsI BbvCI
BclI BfrBI BlpI BmrI BplI BpmI Bpu10I BsaI
BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
BsmBI Bsp1286I BspMI BsrBI BsrFI BsrGI BssSI BstAPI
BstBI BstXI BstYI BtgI BtsI DrdI EaeI EarI
EciI EcoICRI Eco57MI EcoO109I FalI HaeII Hin4I HindIII
MmeI MscI MslI MspA1I NaeI NgoMIV NsiI NspI
PasI PpuMI PvuII SacI SacII SapI SexAI StyI
TaqII TatI TstI Tth111I XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AhdI AleI AloI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BbeI BcgI BciVI
BglI BglII Bme1580I BmgBI BmtI BpuEI BsaBI BsiEI
BsiWI BsmI BspEI BspHI BsrDI BssHII BstEII BstZ17I
Bsu36I BtgZI ClaI CspCI DraI DraIII EagI EcoNI
EcoRI EcoRV FseI FspI FspAI HincII HpaI KasI
KpnI MfeI MluI NarI NcoI NdeI NheI NotI
NruI PacI PciI PflMI PfoI PmeI PmlI PpiI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII
SalI SanDI SbfI ScaI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI SwaI XbaI XhoI XmaI ZraI