Restriction map for
pFN21AE1979
(Linear) (Six-base) MAPSORT of: KIEE1979.seq Check: 7751 from: 1 to: 1671
KIEE1979.orf
With 182 enzymes: SgfI *
July 15, 2010 18:52 ..
Acc65I G'GTAC_C
Cuts at: 0 371 1671
Size: 371 1300
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 370 668 1269 1671
Size: 370 298 601 402
Fragments arranged by size:
601 402 370 298
AfeI AGC'GCT
Cuts at: 0 124 1671
Size: 124 1547
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 488 1671
Size: 488 1183
AlwNI CAG_nnn'CTG
Cuts at: 0 1310 1671
Size: 1310 361
ApoI r'AATT_y
Cuts at: 0 51 1015 1671
Size: 51 964 656
Fragments arranged by size:
964 656 51
AseI AT'TA_AT
Cuts at: 0 1139 1671
Size: 1139 532
BanI G'GyrC_C
Cuts at: 0 182 371 1671
Size: 182 189 1300
Fragments arranged by size:
1300 189 182
BanII G_rGCy'C
Cuts at: 0 86 1671
Size: 86 1585
BclI T'GATC_A
Cuts at: 0 1086 1671
Size: 1086 585
BglI GCCn_nnn'nGGC
Cuts at: 0 1313 1434 1671
Size: 1313 121 237
Fragments arranged by size:
1313 237 121
BlpI GC'TnA_GC
Cuts at: 0 653 1671
Size: 653 1018
Bme1580I G_kGCm'C
Cuts at: 0 185 1271 1671
Size: 185 1086 400
Fragments arranged by size:
1086 400 185
BmrI ACTGGGnnnn_n'
Cuts at: 0 80 568 1671
Size: 80 488 1103
Fragments arranged by size:
1103 488 80
BmtI G_CTAG'C
Cuts at: 0 844 1671
Size: 844 827
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 325 1428 1671
Size: 325 1103 243
Fragments arranged by size:
1103 325 243
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1210 1671
Size: 1210 461
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 956 1671
Size: 956 715
BsaI GGTCTCn'nnnn_
Cuts at: 0 1020 1483 1671
Size: 1020 463 188
Fragments arranged by size:
1020 463 188
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1415 1671
Size: 1415 256
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 430 1671
Size: 430 1241
BsiHKAI G_wGCw'C
Cuts at: 0 746 1671
Size: 746 925
BsmI GAATG_Cn'
Cuts at: 0 726 1599 1671
Size: 726 873 72
Fragments arranged by size:
873 726 72
Bsp1286I G_dGCh'C
Cuts at: 0 86 185 746 1271 1671
Size: 86 99 561 525 400
Fragments arranged by size:
561 525 400 99 86
BspHI T'CATG_A
Cuts at: 0 546 1671
Size: 546 1125
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 719 1671
Size: 719 952
BsrBI CCG'CTC
Cuts at: 0 111 1671
Size: 111 1560
BsrDI GCAATG_nn'
Cuts at: 0 339 1547 1671
Size: 339 1208 124
Fragments arranged by size:
1208 339 124
BsrFI r'CCGG_y
Cuts at: 0 368 1319 1671
Size: 368 951 352
Fragments arranged by size:
951 368 352
BstBI TT'CG_AA
Cuts at: 0 489 1671
Size: 489 1182
BstEII G'GTnAC_C
Cuts at: 0 1443 1671
Size: 1443 228
BstXI CCAn_nnnn'nTGG
Cuts at: 0 72 1671
Size: 72 1599
BstYI r'GATC_y
Cuts at: 0 1665 1671
Size: 1665 6
BtgI C'CryG_G
Cuts at: 0 1643 1671
Size: 1643 28
BtsI GCAGTG_nn'
Cuts at: 0 198 397 418 1429 1671
Size: 198 199 21 1011 242
Fragments arranged by size:
1011 242 199 198 21
DraI TTT'AAA
Cuts at: 0 282 1182 1524 1671
Size: 282 900 342 147
Fragments arranged by size:
900 342 282 147
DraIII CAC_nnn'GTG
Cuts at: 0 150 1275 1671
Size: 150 1125 396
Fragments arranged by size:
1125 396 150
EaeI y'GGCC_r
Cuts at: 0 1426 1640 1671
Size: 1426 214 31
Fragments arranged by size:
1426 214 31
EarI CTCTTCn'nnn_
Cuts at: 0 8 113 1103 1166 1243 1671
Size: 8 105 990 63 77 428
Fragments arranged by size:
990 428 105 77 63 8
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 34 1671
Size: 34 1637
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 370 668 1210 1269 1671
Size: 370 298 542 59 402
Fragments arranged by size:
542 402 370 298 59
EcoRI G'AATT_C
Cuts at: 0 51 1671
Size: 51 1620
HaeII r_GCGC'y
Cuts at: 0 126 1671
Size: 126 1545
HincII GTy'rAC
Cuts at: 0 999 1671
Size: 999 672
HindIII A'AGCT_T
Cuts at: 0 909 1671
Size: 909 762
HpaI GTT'AAC
Cuts at: 0 999 1671
Size: 999 672
KpnI G_GTAC'C
Cuts at: 0 375 1671
Size: 375 1296
MfeI C'AATT_G
Cuts at: 0 288 591 1671
Size: 288 303 1080
Fragments arranged by size:
1080 303 288
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 95 1671
Size: 95 1576
MscI TGG'CCA
Cuts at: 0 1428 1671
Size: 1428 243
MslI CAynn'nnrTG
Cuts at: 0 470 1671
Size: 470 1201
MspA1I CmG'CkG
Cuts at: 0 1158 1310 1671
Size: 1158 152 361
Fragments arranged by size:
1158 361 152
NheI G'CTAG_C
Cuts at: 0 840 1671
Size: 840 831
NspI r_CATG'y
Cuts at: 0 750 1671
Size: 750 921
PflMI CCAn_nnn'nTGG
Cuts at: 0 105 1671
Size: 105 1566
PfoI T'CCnGG_A
Cuts at: 0 339 1671
Size: 339 1332
PstI C_TGCA'G
Cuts at: 0 716 1671
Size: 716 955
PvuII CAG'CTG
Cuts at: 0 1158 1671
Size: 1158 513
SapI GCTCTTCn'nnn_
Cuts at: 0 8 113 1166 1671
Size: 8 105 1053 505
Fragments arranged by size:
1053 505 105 8
SfcI C'TryA_G
Cuts at: 0 245 538 712 1671
Size: 245 293 174 959
Fragments arranged by size:
959 293 245 174
SmlI C'TyrA_G
Cuts at: 0 971 1671
Size: 971 700
SspI AAT'ATT
Cuts at: 0 1143 1671
Size: 1143 528
StyI C'CwwG_G
Cuts at: 0 71 353 1671
Size: 71 282 1318
Fragments arranged by size:
1318 282 71
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 544 576 1261 1293 1671
Size: 544 32 685 32 378
Fragments arranged by size:
685 544 378 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 175 360 1671
Size: 175 185 1311
Fragments arranged by size:
1311 185 175
Enzymes that do cut:
Acc65I AcuI AfeI AlfI AlwNI ApoI AseI BanI
BanII BclI BglI BlpI Bme1580I BmrI BmtI BplI
BpmI BpuEI BsaI BseRI BsgI BsiHKAI BsmI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BstBI BstEII BstXI
BstYI BtgI BtsI DraI DraIII EaeI EarI EciI
Eco57MI EcoRI HaeII HincII HindIII HpaI KpnI MfeI
MmeI MscI MslI MspA1I NheI NspI PflMI PfoI
PstI PvuII SapI SfcI SmlI SspI StyI TstI
XcmI
Enzymes that do not cut:
AarI AatII AccI AclI AflII AflIII AgeI AhdI
AleI AloI ApaI ApaLI AscI AsiSI AvaI AvrII
BaeI BamHI BbeI BbsI BbvCI BcgI BciVI BfrBI
BglII BmgBI Bpu10I BsaAI BsaBI BsaHI BsaWI BsaXI
BseYI BsiEI BsiWI BsmBI BspEI BsrGI BssHII BssSI
BstAPI BstZ17I Bsu36I BtgZI ClaI CspCI DrdI EagI
EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspI FspAI
Hin4I KasI MluI NaeI NarI NcoI NdeI NgoMIV
NotI NruI NsiI PacI PasI PciI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI
RsrII SacI SacII SalI SanDI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI StuI SwaI TaqII TatI Tth111I XbaI XhoI
XmaI XmnI ZraI