Restriction map for
pFN21AE1976
(Linear) (Six-base) MAPSORT of: KIEE1976.seq Check: 9583 from: 1 to: 1608
KIEE1976.orf
With 182 enzymes: SgfI *
July 15, 2010 18:52 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 254 1189 1608
Size: 254 935 419
Fragments arranged by size:
935 419 254
AgeI A'CCGG_T
Cuts at: 0 655 1608
Size: 655 953
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 131 1234 1608
Size: 131 1103 374
Fragments arranged by size:
1103 374 131
AlwNI CAG_nnn'CTG
Cuts at: 0 444 594 765 1017 1608
Size: 444 150 171 252 591
Fragments arranged by size:
591 444 252 171 150
ApaI G_GGCC'C
Cuts at: 0 1107 1116 1250 1608
Size: 1107 9 134 358
Fragments arranged by size:
1107 358 134 9
ApoI r'AATT_y
Cuts at: 0 1118 1399 1608
Size: 1118 281 209
Fragments arranged by size:
1118 281 209
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1349 1382 1608
Size: 1349 33 226
Fragments arranged by size:
1349 226 33
BanI G'GyrC_C
Cuts at: 0 1271 1608
Size: 1271 337
BanII G_rGCy'C
Cuts at: 0 531 1004 1075 1107 1116 1193 1250 1608
Size: 531 473 71 32 9 77 57 358
Fragments arranged by size:
531 473 358 77 71 57 32 9
BbsI GAAGACnn'nnnn_
Cuts at: 0 309 1608
Size: 309 1299
BclI T'GATC_A
Cuts at: 0 395 1608
Size: 395 1213
BglI GCCn_nnn'nGGC
Cuts at: 0 932 1111 1608
Size: 932 179 497
Fragments arranged by size:
932 497 179
BlpI GC'TnA_GC
Cuts at: 0 262 1608
Size: 262 1346
Bme1580I G_kGCm'C
Cuts at: 0 862 885 1107 1116 1250 1608
Size: 862 23 222 9 134 358
Fragments arranged by size:
862 358 222 134 23 9
BmgBI CAC'GTC
Cuts at: 0 945 1608
Size: 945 663
BmrI ACTGGGnnnn_n'
Cuts at: 0 1348 1608
Size: 1348 260
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1089 1608
Size: 1089 519
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 481 1608
Size: 481 1127
BsaI GGTCTCn'nnnn_
Cuts at: 0 360 571 1568 1608
Size: 360 211 997 40
Fragments arranged by size:
997 360 211 40
BsaAI yAC'GTr
Cuts at: 0 568 1608
Size: 568 1040
BsaWI w'CCGG_w
Cuts at: 0 409 655 1608
Size: 409 246 953
Fragments arranged by size:
953 409 246
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 859 889 1159 1189 1608
Size: 859 30 270 30 419
Fragments arranged by size:
859 419 270 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 195 478 559 964 1081 1608
Size: 195 283 81 405 117 527
Fragments arranged by size:
527 405 283 195 117 81
BseYI C'CCAG_C
Cuts at: 0 619 718 737 758 774 1243 1254 1608
Size: 619 99 19 21 16 469 11 354
Fragments arranged by size:
619 469 354 99 21 19 16 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 870 1150 1530 1608
Size: 870 280 380 78
Fragments arranged by size:
870 380 280 78
BsiHKAI G_wGCw'C
Cuts at: 0 531 960 1075 1380 1608
Size: 531 429 115 305 228
Fragments arranged by size:
531 429 305 228 115
BsmBI CGTCTCn'nnnn_
Cuts at: 0 18 218 1608
Size: 18 200 1390
Fragments arranged by size:
1390 200 18
Bsp1286I G_dGCh'C
Cuts at: 0 531 862 885 960 1004 1075 1107 1116
Size: 531 331 23 75 44 71 32 9
Cuts at: 1116 1193 1250 1380 1608
Size: 77 57 130 228
Fragments arranged by size:
531 331 228 130 77 75 71 57
44 32 23 9
BspHI T'CATG_A
Cuts at: 0 41 1608
Size: 41 1567
BsrBI CCG'CTC
Cuts at: 0 9 1608
Size: 9 1599
BsrDI GCAATG_nn'
Cuts at: 0 626 1608
Size: 626 982
BsrFI r'CCGG_y
Cuts at: 0 509 655 1608
Size: 509 146 953
Fragments arranged by size:
953 509 146
BssSI C'ACGA_G
Cuts at: 0 53 503 1608
Size: 53 450 1105
Fragments arranged by size:
1105 450 53
BstAPI GCAn_nnn'nTGC
Cuts at: 0 594 857 1608
Size: 594 263 751
Fragments arranged by size:
751 594 263
Bsu36I CC'TnA_GG
Cuts at: 0 918 1608
Size: 918 690
BtgI C'CryG_G
Cuts at: 0 62 295 806 1608
Size: 62 233 511 802
Fragments arranged by size:
802 511 233 62
BtsI GCAGTG_nn'
Cuts at: 0 433 1608
Size: 433 1175
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 641 676 1608
Size: 641 35 932
Fragments arranged by size:
932 641 35
DraIII CAC_nnn'GTG
Cuts at: 0 827 1375 1608
Size: 827 548 233
Fragments arranged by size:
827 548 233
EaeI y'GGCC_r
Cuts at: 0 164 446 507 645 781 1608
Size: 164 282 61 138 136 827
Fragments arranged by size:
827 282 164 138 136 61
EarI CTCTTCn'nnn_
Cuts at: 0 173 1608
Size: 173 1435
EcoICRI GAG'CTC
Cuts at: 0 529 1073 1608
Size: 529 544 535
Fragments arranged by size:
544 535 529
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 254 481 1189 1608
Size: 254 227 708 419
Fragments arranged by size:
708 419 254 227
EcoNI CCTnn'n_nnAGG
Cuts at: 0 463 1222 1279 1608
Size: 463 759 57 329
Fragments arranged by size:
759 463 329 57
EcoO109I rG'GnC_Cy
Cuts at: 0 288 441 1103 1104 1112 1215 1247 1469
Size: 288 153 662 1 8 103 32 222
Cuts at: 1469 1490 1608
Size: 21 118
Fragments arranged by size:
662 288 222 153 118 103 32 21
8 1
HaeII r_GCGC'y
Cuts at: 0 612 843 1608
Size: 612 231 765
Fragments arranged by size:
765 612 231
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1159 1191 1608
Size: 1159 32 417
Fragments arranged by size:
1159 417 32
HincII GTy'rAC
Cuts at: 0 1029 1608
Size: 1029 579
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1495 1608
Size: 1495 113
MscI TGG'CCA
Cuts at: 0 166 448 647 1608
Size: 166 282 199 961
Fragments arranged by size:
961 282 199 166
MslI CAynn'nnrTG
Cuts at: 0 1426 1608
Size: 1426 182
MspA1I CmG'CkG
Cuts at: 0 619 765 1414 1608
Size: 619 146 649 194
Fragments arranged by size:
649 619 194 146
NaeI GCC'GGC
Cuts at: 0 511 1608
Size: 511 1097
NcoI C'CATG_G
Cuts at: 0 295 806 1608
Size: 295 511 802
Fragments arranged by size:
802 511 295
NdeI CA'TA_TG
Cuts at: 0 342 1608
Size: 342 1266
NgoMIV G'CCGG_C
Cuts at: 0 509 1608
Size: 509 1099
NspI r_CATG'y
Cuts at: 0 118 564 1608
Size: 118 446 1044
Fragments arranged by size:
1044 446 118
PasI CC'CwG_GG
Cuts at: 0 1004 1017 1061 1608
Size: 1004 13 44 547
Fragments arranged by size:
1004 547 44 13
PflMI CCAn_nnn'nTGG
Cuts at: 0 765 894 1017 1608
Size: 765 129 123 591
Fragments arranged by size:
765 591 129 123
PfoI T'CCnGG_A
Cuts at: 0 1544 1608
Size: 1544 64
PpuMI rG'GwC_Cy
Cuts at: 0 288 441 1469 1490 1608
Size: 288 153 1028 21 118
Fragments arranged by size:
1028 288 153 118 21
PspOMI G'GGCC_C
Cuts at: 0 1103 1112 1246 1608
Size: 1103 9 134 362
Fragments arranged by size:
1103 362 134 9
PstI C_TGCA'G
Cuts at: 0 374 440 521 587 1226 1608
Size: 374 66 81 66 639 382
Fragments arranged by size:
639 382 374 81 66 66
PvuII CAG'CTG
Cuts at: 0 619 765 1414 1608
Size: 619 146 649 194
Fragments arranged by size:
649 619 194 146
SacI G_AGCT'C
Cuts at: 0 531 1075 1608
Size: 531 544 533
Fragments arranged by size:
544 533 531
SexAI A'CCwGG_T
Cuts at: 0 1371 1608
Size: 1371 237
SfcI C'TryA_G
Cuts at: 0 370 436 517 583 1222 1231 1608
Size: 370 66 81 66 639 9 377
Fragments arranged by size:
639 377 370 81 66 66 9
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1111 1608
Size: 1111 497
SphI G_CATG'C
Cuts at: 0 118 1608
Size: 118 1490
StuI AGG'CCT
Cuts at: 0 161 196 1608
Size: 161 35 1412
Fragments arranged by size:
1412 161 35
StyI C'CwwG_G
Cuts at: 0 295 806 1107 1275 1337 1608
Size: 295 511 301 168 62 271
Fragments arranged by size:
511 301 295 271 168 62
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1550 1608
Size: 1550 58
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 859 891 1608
Size: 859 32 717
Fragments arranged by size:
859 717 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 456 1086 1379 1608
Size: 456 630 293 229
Fragments arranged by size:
630 456 293 229
Enzymes that do cut:
AcuI AgeI AlfI AlwNI ApaI ApoI BaeI BanI
BanII BbsI BclI BglI BlpI Bme1580I BmgBI BmrI
BplI BpmI BsaI BsaAI BsaWI BsaXI BseRI BseYI
BsgI BsiHKAI BsmBI Bsp1286I BspHI BsrBI BsrDI BsrFI
BssSI BstAPI Bsu36I BtgI BtsI CspCI DraIII EaeI
EarI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I HincII
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NspI PasI PflMI PfoI PpuMI PspOMI PstI PvuII
SacI SexAI SfcI SfiI SphI StuI StyI TaqII
TstI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AhdI AleI AloI ApaLI AscI AseI AsiSI AvaI
AvrII BamHI BbeI BbvCI BcgI BciVI BfrBI BglII
BmtI Bpu10I BpuEI BsaBI BsaHI BsiEI BsiWI BsmI
BspEI BspMI BsrGI BssHII BstBI BstEII BstXI BstYI
BstZ17I BtgZI ClaI DraI DrdI EagI EciI EcoRI
EcoRV FalI FseI FspI FspAI HindIII HpaI KasI
KpnI MfeI MluI NarI NheI NotI NruI NsiI
PacI PciI PmeI PmlI PpiI PshAI PsiI PspXI
PsrI PvuI RsrII SacII SalI SanDI SapI SbfI
ScaI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SrfI SspI SwaI TatI Tth111I XbaI XhoI XmaI
XmnI ZraI