Restriction map for
pFN21AE1973
(Linear) (Six-base) MAPSORT of: KIEE1973.seq Check: 5901 from: 1 to: 1623
KIEE1973.orf
With 182 enzymes: SgfI *
July 15, 2010 18:52 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1513 1623
Size: 1513 110
AflIII A'CryG_T
Cuts at: 0 192 1623
Size: 192 1431
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1041 1199 1623
Size: 1041 158 424
Fragments arranged by size:
1041 424 158
ApoI r'AATT_y
Cuts at: 0 400 1423 1623
Size: 400 1023 200
Fragments arranged by size:
1023 400 200
AvaI C'yCGr_G
Cuts at: 0 219 1623
Size: 219 1404
BamHI G'GATC_C
Cuts at: 0 771 1623
Size: 771 852
BbsI GAAGACnn'nnnn_
Cuts at: 0 969 1317 1623
Size: 969 348 306
Fragments arranged by size:
969 348 306
BclI T'GATC_A
Cuts at: 0 992 1623
Size: 992 631
BfrBI ATG'CAT
Cuts at: 0 1238 1623
Size: 1238 385
BmgBI CAC'GTC
Cuts at: 0 73 975 1623
Size: 73 902 648
Fragments arranged by size:
902 648 73
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 508 797 1283 1623
Size: 508 289 486 340
Fragments arranged by size:
508 486 340 289
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1512 1623
Size: 1512 111
BsaWI w'CCGG_w
Cuts at: 0 642 1593 1623
Size: 642 951 30
Fragments arranged by size:
951 642 30
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1256 1259 1286 1289 1623
Size: 1256 3 27 3 334
Fragments arranged by size:
1256 334 27 3 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1186 1280 1623
Size: 1186 94 343
Fragments arranged by size:
1186 343 94
BsiHKAI G_wGCw'C
Cuts at: 0 1178 1623
Size: 1178 445
BsmI GAATG_Cn'
Cuts at: 0 1156 1623
Size: 1156 467
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1580 1623
Size: 1580 43
Bsp1286I G_dGCh'C
Cuts at: 0 1178 1623
Size: 1178 445
BsrDI GCAATG_nn'
Cuts at: 0 117 1623
Size: 117 1506
BssSI C'ACGA_G
Cuts at: 0 123 1623
Size: 123 1500
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1209 1623
Size: 1209 414
BstYI r'GATC_y
Cuts at: 0 280 771 1623
Size: 280 491 852
Fragments arranged by size:
852 491 280
BtsI GCAGTG_nn'
Cuts at: 0 50 183 683 1623
Size: 50 133 500 940
Fragments arranged by size:
940 500 133 50
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 159 194 1623
Size: 159 35 1429
Fragments arranged by size:
1429 159 35
EaeI y'GGCC_r
Cuts at: 0 256 899 1623
Size: 256 643 724
Fragments arranged by size:
724 643 256
EarI CTCTTCn'nnn_
Cuts at: 0 722 782 1086 1623
Size: 722 60 304 537
Fragments arranged by size:
722 537 304 60
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 508 797 1283 1513 1623
Size: 508 289 486 230 110
Fragments arranged by size:
508 486 289 230 110
EcoRV GAT'ATC
Cuts at: 0 963 1623
Size: 963 660
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 265 297 977 1009 1623
Size: 265 32 680 32 614
Fragments arranged by size:
680 614 265 32 32
HincII GTy'rAC
Cuts at: 0 447 1059 1623
Size: 447 612 564
Fragments arranged by size:
612 564 447
HindIII A'AGCT_T
Cuts at: 0 661 927 1468 1623
Size: 661 266 541 155
Fragments arranged by size:
661 541 266 155
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1190 1623
Size: 1190 433
MscI TGG'CCA
Cuts at: 0 258 901 1623
Size: 258 643 722
Fragments arranged by size:
722 643 258
MslI CAynn'nnrTG
Cuts at: 0 85 239 572 937 1623
Size: 85 154 333 365 686
Fragments arranged by size:
686 365 333 154 85
MspA1I CmG'CkG
Cuts at: 0 353 1623
Size: 353 1270
NdeI CA'TA_TG
Cuts at: 0 1122 1623
Size: 1122 501
NsiI A_TGCA'T
Cuts at: 0 1240 1623
Size: 1240 383
NspI r_CATG'y
Cuts at: 0 196 832 1623
Size: 196 636 791
Fragments arranged by size:
791 636 196
PasI CC'CwG_GG
Cuts at: 0 1202 1623
Size: 1202 421
PciI A'CATG_T
Cuts at: 0 192 1623
Size: 192 1431
PflMI CCAn_nnn'nTGG
Cuts at: 0 164 1623
Size: 164 1459
PsiI TTA'TAA
Cuts at: 0 1523 1623
Size: 1523 100
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1534 1566 1623
Size: 1534 32 57
Fragments arranged by size:
1534 57 32
PstI C_TGCA'G
Cuts at: 0 57 1623
Size: 57 1566
PvuII CAG'CTG
Cuts at: 0 353 1623
Size: 353 1270
SfcI C'TryA_G
Cuts at: 0 53 1623
Size: 53 1570
SmlI C'TyrA_G
Cuts at: 0 219 1527 1623
Size: 219 1308 96
Fragments arranged by size:
1308 219 96
SpeI A'CTAG_T
Cuts at: 0 376 1623
Size: 376 1247
SphI G_CATG'C
Cuts at: 0 832 1623
Size: 832 791
SspI AAT'ATT
Cuts at: 0 858 1623
Size: 858 765
StyI C'CwwG_G
Cuts at: 0 208 1623
Size: 208 1415
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 21 1623
Size: 21 1602
TatI w'GTAC_w
Cuts at: 0 64 1623
Size: 64 1559
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 821 1623
Size: 821 802
XhoI C'TCGA_G
Cuts at: 0 219 1623
Size: 219 1404
Enzymes that do cut:
AcuI AflIII AlfI ApoI AvaI BamHI BbsI BclI
BfrBI BmgBI BpmI BpuEI BsaWI BsaXI BseRI BsiHKAI
BsmI BsmBI Bsp1286I BsrDI BssSI BstXI BstYI BtsI
CspCI EaeI EarI Eco57MI EcoRV Hin4I HincII HindIII
MmeI MscI MslI MspA1I NdeI NsiI NspI PasI
PciI PflMI PsiI PsrI PstI PvuII SfcI SmlI
SpeI SphI SspI StyI TaqII TatI XcmI XhoI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AloI AlwNI ApaI ApaLI AscI AseI
AsiSI AvrII BaeI BanI BanII BbeI BbvCI BcgI
BciVI BglI BglII BlpI Bme1580I BmrI BmtI BplI
Bpu10I BsaI BsaAI BsaBI BsaHI BseYI BsgI BsiEI
BsiWI BspEI BspHI BspMI BsrBI BsrFI BsrGI BssHII
BstAPI BstBI BstEII BstZ17I Bsu36I BtgI BtgZI ClaI
DraI DraIII DrdI EagI EciI EcoICRI EcoNI EcoO109I
EcoRI FalI FseI FspI FspAI HaeII HpaI KasI
KpnI MfeI MluI NaeI NarI NcoI NgoMIV NheI
NotI NruI PacI PfoI PmeI PmlI PpiI PpuMI
PshAI PspOMI PspXI PvuI RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SrfI StuI SwaI TstI Tth111I
XbaI XmaI XmnI ZraI