Restriction map for
pFN21AE1972
(Linear) (Six-base) MAPSORT of: KIEE1972.seq Check: 7543 from: 1 to: 1620
KIEE1972.orf
With 182 enzymes: SgfI *
July 15, 2010 18:52 ..
AccI GT'mk_AC
Cuts at: 0 1436 1620
Size: 1436 184
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 692 1620
Size: 692 928
AflII C'TTAA_G
Cuts at: 0 403 1620
Size: 403 1217
AlwNI CAG_nnn'CTG
Cuts at: 0 939 1620
Size: 939 681
ApoI r'AATT_y
Cuts at: 0 233 352 1620
Size: 233 119 1268
Fragments arranged by size:
1268 233 119
BamHI G'GATC_C
Cuts at: 0 486 1620
Size: 486 1134
BanI G'GyrC_C
Cuts at: 0 1234 1428 1521 1620
Size: 1234 194 93 99
Fragments arranged by size:
1234 194 99 93
BbeI G_GCGC'C
Cuts at: 0 1432 1620
Size: 1432 188
BbsI GAAGACnn'nnnn_
Cuts at: 0 130 680 844 1279 1298 1620
Size: 130 550 164 435 19 322
Fragments arranged by size:
550 435 322 164 130 19
BbvCI CC'TCA_GC
Cuts at: 0 68 1556 1583 1620
Size: 68 1488 27 37
Fragments arranged by size:
1488 68 37 27
BciVI GTATCCnnnnn_n'
Cuts at: 0 602 1620
Size: 602 1018
BclI T'GATC_A
Cuts at: 0 963 1620
Size: 963 657
BglII A'GATC_T
Cuts at: 0 798 1620
Size: 798 822
BmrI ACTGGGnnnn_n'
Cuts at: 0 84 950 1620
Size: 84 866 670
Fragments arranged by size:
866 670 84
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 719 1258 1620
Size: 719 539 362
Fragments arranged by size:
719 539 362
Bpu10I CC'TnA_GC
Cuts at: 0 68 1556 1583 1620
Size: 68 1488 27 37
Fragments arranged by size:
1488 68 37 27
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 535 1620
Size: 535 1085
BsaHI Gr'CG_yC
Cuts at: 0 1429 1620
Size: 1429 191
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 200 230 1288 1318 1620
Size: 200 30 1058 30 302
Fragments arranged by size:
1058 302 200 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1214 1436 1620
Size: 1214 222 184
Fragments arranged by size:
1214 222 184
BseYI C'CCAG_C
Cuts at: 0 426 468 1344 1420 1620
Size: 426 42 876 76 200
Fragments arranged by size:
876 426 200 76 42
BsmI GAATG_Cn'
Cuts at: 0 1503 1620
Size: 1503 117
BsrDI GCAATG_nn'
Cuts at: 0 395 1007 1620
Size: 395 612 613
Fragments arranged by size:
613 612 395
BstYI r'GATC_y
Cuts at: 0 171 486 798 1131 1620
Size: 171 315 312 333 489
Fragments arranged by size:
489 333 315 312 171
BstZ17I GTA'TAC
Cuts at: 0 1437 1620
Size: 1437 183
Bsu36I CC'TnA_GG
Cuts at: 0 128 1620
Size: 128 1492
BtsI GCAGTG_nn'
Cuts at: 0 803 1328 1620
Size: 803 525 292
Fragments arranged by size:
803 525 292
DraI TTT'AAA
Cuts at: 0 693 1620
Size: 693 927
EarI CTCTTCn'nnn_
Cuts at: 0 150 206 1620
Size: 150 56 1414
Fragments arranged by size:
1414 150 56
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 692 719 1258 1620
Size: 692 27 539 362
Fragments arranged by size:
692 539 362 27
EcoO109I rG'GnC_Cy
Cuts at: 0 78 1018 1098 1219 1504 1620
Size: 78 940 80 121 285 116
Fragments arranged by size:
940 285 121 116 80 78
EcoRV GAT'ATC
Cuts at: 0 93 1620
Size: 93 1527
HaeII r_GCGC'y
Cuts at: 0 1432 1620
Size: 1432 188
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 783 815 1005 1037 1269 1288 1301 1320
Size: 783 32 190 32 232 19 13 19
Cuts at: 1320 1620
Size: 300
Fragments arranged by size:
783 300 232 190 32 32 19 19
13
HincII GTy'rAC
Cuts at: 0 572 1620
Size: 572 1048
KasI G'GCGC_C
Cuts at: 0 1428 1620
Size: 1428 192
MslI CAynn'nnrTG
Cuts at: 0 626 1620
Size: 626 994
MspA1I CmG'CkG
Cuts at: 0 1302 1620
Size: 1302 318
NarI GG'CG_CC
Cuts at: 0 1429 1620
Size: 1429 191
NspI r_CATG'y
Cuts at: 0 215 1620
Size: 215 1405
PflMI CCAn_nnn'nTGG
Cuts at: 0 433 1323 1620
Size: 433 890 297
Fragments arranged by size:
890 433 297
PpuMI rG'GwC_Cy
Cuts at: 0 1018 1504 1620
Size: 1018 486 116
Fragments arranged by size:
1018 486 116
PstI C_TGCA'G
Cuts at: 0 450 798 1620
Size: 450 348 822
Fragments arranged by size:
822 450 348
PvuII CAG'CTG
Cuts at: 0 1302 1620
Size: 1302 318
SexAI A'CCwGG_T
Cuts at: 0 1241 1620
Size: 1241 379
SfcI C'TryA_G
Cuts at: 0 446 794 931 1281 1620
Size: 446 348 137 350 339
Fragments arranged by size:
446 350 348 339 137
SfoI GGC'GCC
Cuts at: 0 1430 1620
Size: 1430 190
SmlI C'TyrA_G
Cuts at: 0 403 550 1620
Size: 403 147 1070
Fragments arranged by size:
1070 403 147
StyI C'CwwG_G
Cuts at: 0 482 1620
Size: 482 1138
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 131 1620
Size: 131 1489
TatI w'GTAC_w
Cuts at: 0 946 1620
Size: 946 674
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 576 608 1620
Size: 576 32 1012
Fragments arranged by size:
1012 576 32
XmnI GAAnn'nnTTC
Cuts at: 0 1059 1620
Size: 1059 561
Enzymes that do cut:
AccI AcuI AflII AlwNI ApoI BamHI BanI BbeI
BbsI BbvCI BciVI BclI BglII BmrI BpmI Bpu10I
BpuEI BsaHI BsaXI BseRI BseYI BsmI BsrDI BstYI
BstZ17I Bsu36I BtsI DraI EarI Eco57MI EcoO109I EcoRV
HaeII Hin4I HincII KasI MslI MspA1I NarI NspI
PflMI PpuMI PstI PvuII SexAI SfcI SfoI SmlI
StyI TaqII TatI TstI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflIII AgeI AhdI
AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BanII BcgI BfrBI BglI BlpI
Bme1580I BmgBI BmtI BplI BsaI BsaAI BsaBI BsaWI
BsgI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BtgI BtgZI ClaI CspCI DraIII DrdI
EaeI EagI EciI EcoICRI EcoNI EcoRI FalI FseI
FspI FspAI HindIII HpaI KpnI MfeI MluI MmeI
MscI NaeI NcoI NdeI NgoMIV NheI NotI NruI
NsiI PacI PasI PciI PfoI PmeI PmlI PpiI
PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SfiI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI
SwaI Tth111I XbaI XcmI XhoI XmaI ZraI