Restriction map for
pFN21AE1970
(Linear) (Six-base) MAPSORT of: KIEE1970.seq Check: 5720 from: 1 to: 1611
KIEE1970.orf
With 182 enzymes: SgfI *
December 27, 2011 16:31 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 415 1454 1611
Size: 415 1039 157
Fragments arranged by size:
1039 415 157
AflII C'TTAA_G
Cuts at: 0 889 1611
Size: 889 722
AflIII A'CryG_T
Cuts at: 0 467 1345 1505 1611
Size: 467 878 160 106
Fragments arranged by size:
878 467 160 106
AhdI GACnn_n'nnGTC
Cuts at: 0 1300 1611
Size: 1300 311
AlwNI CAG_nnn'CTG
Cuts at: 0 81 868 1339 1611
Size: 81 787 471 272
Fragments arranged by size:
787 471 272 81
ApaLI G'TGCA_C
Cuts at: 0 315 1611
Size: 315 1296
ApoI r'AATT_y
Cuts at: 0 400 439 1244 1439 1536 1611
Size: 400 39 805 195 97 75
Fragments arranged by size:
805 400 195 97 75 39
AvaI C'yCGr_G
Cuts at: 0 30 40 1611
Size: 30 10 1571
Fragments arranged by size:
1571 30 10
BanI G'GyrC_C
Cuts at: 0 36 498 1611
Size: 36 462 1113
Fragments arranged by size:
1113 462 36
BbeI G_GCGC'C
Cuts at: 0 40 1611
Size: 40 1571
BclI T'GATC_A
Cuts at: 0 564 1611
Size: 564 1047
BglI GCCn_nnn'nGGC
Cuts at: 0 350 1611
Size: 350 1261
BlpI GC'TnA_GC
Cuts at: 0 639 1611
Size: 639 972
Bme1580I G_kGCm'C
Cuts at: 0 206 319 503 1389 1611
Size: 206 113 184 886 222
Fragments arranged by size:
886 222 206 184 113
BmrI ACTGGGnnnn_n'
Cuts at: 0 1392 1611
Size: 1392 219
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 561 1611
Size: 561 1050
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 255 1611
Size: 255 1356
Bpu10I CC'TnA_GC
Cuts at: 0 752 1611
Size: 752 859
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 589 1611
Size: 589 1022
BsaI GGTCTCn'nnnn_
Cuts at: 0 58 1611
Size: 58 1553
BsaAI yAC'GTr
Cuts at: 0 1508 1611
Size: 1508 103
BsaBI GATnn'nnATC
Cuts at: 0 331 1611
Size: 331 1280
BsaHI Gr'CG_yC
Cuts at: 0 37 47 221 1611
Size: 37 10 174 1390
Fragments arranged by size:
1390 174 37 10
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 253 283 1611
Size: 253 30 1328
Fragments arranged by size:
1328 253 30
BsiHKAI G_wGCw'C
Cuts at: 0 319 605 1611
Size: 319 286 1006
Fragments arranged by size:
1006 319 286
BsmBI CGTCTCn'nnnn_
Cuts at: 0 369 1611
Size: 369 1242
Bsp1286I G_dGCh'C
Cuts at: 0 206 319 503 605 1389 1611
Size: 206 113 184 102 784 222
Fragments arranged by size:
784 222 206 184 113 102
BspHI T'CATG_A
Cuts at: 0 1008 1611
Size: 1008 603
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1213 1611
Size: 1213 398
BsrBI CCG'CTC
Cuts at: 0 126 252 638 1611
Size: 126 126 386 973
Fragments arranged by size:
973 386 126 126
BsrDI GCAATG_nn'
Cuts at: 0 797 1043 1214 1611
Size: 797 246 171 397
Fragments arranged by size:
797 397 246 171
BsrFI r'CCGG_y
Cuts at: 0 96 1611
Size: 96 1515
BsrGI T'GTAC_A
Cuts at: 0 1328 1611
Size: 1328 283
BssSI C'ACGA_G
Cuts at: 0 205 1611
Size: 205 1406
BstAPI GCAn_nnn'nTGC
Cuts at: 0 81 210 1611
Size: 81 129 1401
Fragments arranged by size:
1401 129 81
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1530 1611
Size: 1530 81
BtsI GCAGTG_nn'
Cuts at: 0 698 1073 1602 1611
Size: 698 375 529 9
Fragments arranged by size:
698 529 375 9
ClaI AT'CG_AT
Cuts at: 0 728 1611
Size: 728 883
DraIII CAC_nnn'GTG
Cuts at: 0 549 1611
Size: 549 1062
EaeI y'GGCC_r
Cuts at: 0 415 941 1611
Size: 415 526 670
Fragments arranged by size:
670 526 415
EarI CTCTTCn'nnn_
Cuts at: 0 116 488 530 848 1505 1555 1611
Size: 116 372 42 318 657 50 56
Fragments arranged by size:
657 372 318 116 56 50 42
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 255 415 1454 1611
Size: 255 160 1039 157
Fragments arranged by size:
1039 255 160 157
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1589 1611
Size: 1589 22
EcoRI G'AATT_C
Cuts at: 0 1536 1611
Size: 1536 75
EcoRV GAT'ATC
Cuts at: 0 660 839 1611
Size: 660 179 772
Fragments arranged by size:
772 660 179
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 904 1611
Size: 904 707
HaeII r_GCGC'y
Cuts at: 0 40 132 759 1611
Size: 40 92 627 852
Fragments arranged by size:
852 627 92 40
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1286 1318 1611
Size: 1286 32 293
Fragments arranged by size:
1286 293 32
KasI G'GCGC_C
Cuts at: 0 36 1611
Size: 36 1575
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1116 1403 1439 1547 1611
Size: 1116 287 36 108 64
Fragments arranged by size:
1116 287 108 64 36
MscI TGG'CCA
Cuts at: 0 417 943 1611
Size: 417 526 668
Fragments arranged by size:
668 526 417
MslI CAynn'nnrTG
Cuts at: 0 151 472 577 1021 1611
Size: 151 321 105 444 590
Fragments arranged by size:
590 444 321 151 105
MspA1I CmG'CkG
Cuts at: 0 234 279 1611
Size: 234 45 1332
Fragments arranged by size:
1332 234 45
NaeI GCC'GGC
Cuts at: 0 98 1611
Size: 98 1513
NarI GG'CG_CC
Cuts at: 0 37 1611
Size: 37 1574
NgoMIV G'CCGG_C
Cuts at: 0 96 1611
Size: 96 1515
NspI r_CATG'y
Cuts at: 0 360 471 1479 1611
Size: 360 111 1008 132
Fragments arranged by size:
1008 360 132 111
PciI A'CATG_T
Cuts at: 0 467 1611
Size: 467 1144
PflMI CCAn_nnn'nTGG
Cuts at: 0 956 1611
Size: 956 655
PmlI CAC'GTG
Cuts at: 0 1508 1611
Size: 1508 103
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 857 889 1611
Size: 857 32 722
Fragments arranged by size:
857 722 32
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 154 186 1611
Size: 154 32 1425
Fragments arranged by size:
1425 154 32
PstI C_TGCA'G
Cuts at: 0 77 215 233 284 436 1224 1407 1611
Size: 77 138 18 51 152 788 183 204
Fragments arranged by size:
788 204 183 152 138 77 51 18
PvuII CAG'CTG
Cuts at: 0 234 279 1611
Size: 234 45 1332
Fragments arranged by size:
1332 234 45
SapI GCTCTTCn'nnn_
Cuts at: 0 116 1611
Size: 116 1495
ScaI AGT'ACT
Cuts at: 0 713 1325 1611
Size: 713 612 286
Fragments arranged by size:
713 612 286
SexAI A'CCwGG_T
Cuts at: 0 194 1611
Size: 194 1417
SfcI C'TryA_G
Cuts at: 0 73 211 229 280 432 821 1220 1403
Size: 73 138 18 51 152 389 399 183
Cuts at: 1403 1611
Size: 208
Fragments arranged by size:
399 389 208 183 152 138 73 51
18
SfoI GGC'GCC
Cuts at: 0 38 1611
Size: 38 1573
SmaI CCC'GGG
Cuts at: 0 32 42 1611
Size: 32 10 1569
Fragments arranged by size:
1569 32 10
SmlI C'TyrA_G
Cuts at: 0 604 889 1611
Size: 604 285 722
Fragments arranged by size:
722 604 285
SspI AAT'ATT
Cuts at: 0 789 1611
Size: 789 822
StyI C'CwwG_G
Cuts at: 0 644 1611
Size: 644 967
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 40 567 1238 1566 1611
Size: 40 527 671 328 45
Fragments arranged by size:
671 527 328 45 40
TatI w'GTAC_w
Cuts at: 0 569 711 926 1323 1328 1611
Size: 569 142 215 397 5 283
Fragments arranged by size:
569 397 283 215 142 5
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 239 271 828 860 1611
Size: 239 32 557 32 751
Fragments arranged by size:
751 557 239 32 32
XmaI C'CCGG_G
Cuts at: 0 30 40 1611
Size: 30 10 1571
Fragments arranged by size:
1571 30 10
XmnI GAAnn'nnTTC
Cuts at: 0 558 869 1611
Size: 558 311 742
Fragments arranged by size:
742 558 311
Enzymes that do cut:
AcuI AflII AflIII AhdI AlwNI ApaLI ApoI AvaI
BanI BbeI BclI BglI BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaXI
BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstXI BtsI ClaI DraIII EaeI
EarI Eco57MI EcoNI EcoRI EcoRV FalI HaeII Hin4I
KasI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PciI PflMI PmlI PpiI PsrI PstI PvuII
SapI ScaI SexAI SfcI SfoI SmaI SmlI SspI
StyI TaqII TatI TstI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AgeI AleI
AlfI AloI ApaI AscI AseI AsiSI AvrII BaeI
BamHI BanII BbsI BbvCI BcgI BciVI BfrBI BglII
BmgBI BmtI BsaWI BseRI BseYI BsgI BsiEI BsiWI
BsmI BspEI BssHII BstBI BstEII BstYI BstZ17I Bsu36I
BtgI BtgZI CspCI DraI DrdI EagI EciI EcoICRI
EcoO109I FseI FspI FspAI HincII HindIII HpaI KpnI
MfeI MluI NcoI NdeI NheI NotI NruI NsiI
PacI PasI PfoI PmeI PpuMI PshAI PsiI PspOMI
PspXI PvuI RsrII SacI SacII SalI SanDI SbfI
SfiI SgfI SgrAI SnaBI SpeI SphI SrfI StuI
SwaI Tth111I XbaI XcmI XhoI ZraI